Effective Disinfection of Trout Eggs to Prevent Egg Transmission of Aeromonas liquefaciens

1969 ◽  
Vol 26 (9) ◽  
pp. 2311-2318 ◽  
Author(s):  
T. W. McFadden
Keyword(s):  
Outer Surface ◽  
Povidone Iodine ◽  
No Reduction ◽  
Aeromonas Salmonicida ◽  
Bacterial Cells ◽  
Surface Disinfection ◽  
Rate Of Development ◽  
Iodine Compound ◽  
Organic Iodine ◽  
Viable Bacteria

Treatment of trout eggs with classical disinfectants such as sulfo-merthiolate, merthiolate, and acriflavine proved unreliable for destroying Aeromonas liquefaciens on the eggshell. These bacteristatic agents washed free leaving viable bacteria on the shell.An organic iodine compound, povidone–iodine, was found to be bactericidal at dosages readily tolerated by trout eggs. No reduction in the rate of development was observed after treatment.Transmission of A. liquefaciens from parents to offspring follows the route reported for Aeromonas salmonicida. Viable bacterial cells are carried on the outer surface of the shell only, enabling the use of surface disinfection.

scholarly journals Binding of Aflatoxin B1 to Lactobacillus paracasei SNP-2 and Stability of Bacteria-AFB1 Complex

2017 ◽  
Vol 14 (1) ◽  
pp. 1 ◽  
Author(s):  
Rohula Utami ◽  
Tyas Utami ◽  
Suparmo Suparmo ◽  
Endang Sutriswati Rahayu
Keyword(s):  
Aflatoxin B1 ◽  
Phosphate Buffer ◽  
Cell Concentration ◽  
Lactobacillus Paracasei ◽  
Complex Stability ◽  
Bacterial Cells ◽  
Binding Ability ◽  
Viable Cells ◽  
Viable Bacteria ◽  
Mrs Broth

The aim of this research was to study the binding ability of viable and non-viable of Lactobacillus paracasei SNP-2 to aflatoxin B1 (AFB1) in phosphate buffer saline (PBS) at pH 7.3. Bacterial cells were grown in MRS broth at 37 °C for 24 h, and then centrifuged at 1,800g[a1]  for 20 min at 10 °C to get the pellet. Pellet was suspended in PBS pH 7.3 until the cell concentration was about 1010 CFU/ml. Viable cells, the heated, and acid-killed cells were evaluated their ability to bind AFB1 in PBS pH 7.3. Stability of the L. paracasei SNP-2/AFB1 complexes was evaluated by determining the amount of the released AFB1 to the PBS following five times washing. The results showed that AFB1 binding ability to heat-and acid-kill bacteria were higher than that of by viable cells. More than 70% of bound AFB1 was released from viable bacteria after five times washing. However, the heated and acid-killed cell treatments significantly increased the complex stability of bacteria-AFB1

scholarly journals Effects of the povidone-iodine (PVPI) in treatment of bacterial peritonitis induced in rats

2010 ◽  
Vol 25 (4) ◽  
pp. 322-327 ◽  
Author(s):  
Ivana Duval de Araujo ◽  
Giovanni Cezar Xavier Grossi ◽  
Simone Odília Fernandes Diniz ◽  
Tarcizo Afonso Nunes ◽  
Eduardo Ângelo Braga ◽  
...  
Keyword(s):  
Peritoneal Cavity ◽  
Saline Solution ◽  
Povidone Iodine ◽  
Absorption Index ◽  
Control Group ◽  
Phagocytic Cells ◽  
Bacterial Peritonitis ◽  
Viable Bacteria ◽  
Group A ◽  
Group B

PURPOSE: To evaluate the effectiveness of the use of the povidone-iodine (PVI) added to the liquid of wash of the peritoneal cavity in the reduction of bacterial absorption and in the remainder non-phagocyted bacteria in the circulating blood of rat. METHODS: Thirty four Wistar females rats were used, distributed in the following groups: A (n=10), non-treated; B (n=9), wash of the peritoneal cavity with solution of PVI to 1% in saline solution; C (n=15), wash of the cavity with saline solution. After anesthesia, it was made intraperitoneal infusion of solution of Escherichia coli labeled with 99mTc containing 10(8) CFU/ml. After 40 minutes, it was made the treatment, in the group A, manipulation of the viscera; in the group B, irrigation of the peritoneal cavity with warm solution of 1% PVPI to 37,5ºC, and in the group C irrigation with warm saline (37,5ºC). After 15 minutes of the treatment, blood samples and fragments of liver, spleen and lung was obtained for count of the radioactivity, and animals killed by abdominal aorta section. There were determined the bacterial absorption index and the remainder index in the bloodstream. RESULTS: Of the total of bacteria infused in the peritoneum, there was absorption of 0,92% (0,14% to 2,13%) in the animals of the group A (controls), 0,49% (0,18% to 0,71%) after use of topical PVPI (group B) and 0,80% (0,04% to 3,8%) after wash with saline solution (group C). There was significant reduction of the absorption when compared the treated animals with PVPI and the controls (p=0,003). Of the total of bacteria absorbed for the circulatory current, the percentile amount of bacteria non-phagocyted in the outlying blood was of 2,9% (1,1% to 17,7%) in the control group, 15,2% (8,3% to 21,4%) in those treated with PVPI (group B) and 6,9% (0,8% to 29,7%) after wash with saline solution (group C), with difference among controls and treated with PVPI (p=0,01). CONCLUSION: The wash of the cavity peritoneal of mice with solution containing PVPI showed to be capable to reduce the absorption of bacteria by peritoneum of rat; however it seems to interfere with the function of the phagocytic cells for the observation of the increase of viable bacteria in the outlying blood of those animals.

Effect of Cooking on Bacteriological Populations of “Soul Foods”

1983 ◽  
Vol 46 (1) ◽  
pp. 19-20 ◽  
Author(s):  
ADELLE W. STEWART
Keyword(s):  
Plate Count ◽  
Bacterial Cells ◽  
Viable Bacteria ◽  
Aerobic Plate Count

Effect of typical cooking procedures on viable bacteria present in “soul foods” was determined. Time and temperature used were those determined to give a satisfactory product. Except for an aerobic plate count of 60 CFU/g in puddings (liver) and 10 CFU/g in jaws and neckbones, survival of vegetative bacterial cells in the cooked foods was nil.

ACCELERATION OF DEVELOPMENT OF LARVAE OF THE ONION MAGGOT, HYLEMYA ANTIQUA (MEIG.), BY MICROORGANISMS

1959 ◽  
Vol 37 (5) ◽  
pp. 721-727 ◽  
Author(s):  
W. G. Friend ◽  
E. H. Salkeld ◽  
I. L. Stevenson
Keyword(s):  
Growth Rate ◽  
Bacterial Cells ◽  
Onion Maggot ◽  
Hylemya Antiqua ◽  
Rate Of Development ◽  
Sonic Treatment ◽  
Living Bacteria

On a chemical diet, larvae of H. antiqua developed more rapidly in the presence of various living bacteria, of certain nonviable bacterial cells, or of the dialyzate of these cells. The stimulation by the nonviable cells was not affected when the cells were disrupted by sonic treatment. Additions of ashed cells to the chemical diet had no stimulatory effect. Some stimulation in rate of development was noted on addition of casein to the diets but to a lesser degree than that caused by equivalent concentrations of nonviable cells. The growth rate and the rate of metamorphosis stayed in phase with one another when development was accelerated.

scholarly journals Clinical Evaluation of Povidone-Iodine Compound Dentifrice.

1993 ◽  
Vol 35 (4) ◽  
pp. 711-718
Author(s):  
Hisashi WATANABE ◽  
Isao ISHIKAWA ◽  
Hiroaki KOKATSU ◽  
Hajime MIYASHITA ◽  
Joichiro SUZUKI ◽  
...  
Keyword(s):  
Clinical Evaluation ◽  
Povidone Iodine ◽  
Iodine Compound

scholarly journals Sensitivity of biofilm and planktonic forms of non-fermenting bacteria to the action of antiseptics

2018 ◽  
Vol 22 (2) ◽  
pp. 293-296
Author(s):  
Y.Y. Trofimenko ◽  
E.F. Makac ◽  
O.K. Stucan ◽  
V.M. Burkot
Keyword(s):  
Hydrogen Peroxide ◽  
Povidone Iodine ◽  
Bacterial Cells ◽  
Gram Negative ◽  
Endotracheal Tubes ◽  
Bacterial Films ◽  
Fermenting Bacteria ◽  
Planktonic Form ◽  
Bacterial Film ◽  
Peptone Broth

It is known that bacteria colonize endotracheal intubation tubes [EIT], form biofilms. Bacterial cells that are in the film matrix are protected from the effects of external factors and differ in high levels of resistance to the action of antibiotics, antiseptics, and disinfectants. The purpose of our study was to determine the sensitivity of the antiseptics of biofilm and planktont forms of non-fermentable gram-negative bacteria to the action of the antiseptics of decamethoxin, chlorohexidine bigluconate, povidone iodine, hydrogen peroxide. In order to create bacterial biofilms, sterile fragments of the endotracheal tubes were placed in test tubes with meat-peptone broth and the daily culture of the studied strain of microorganisms was blocked. To determine the influence of antiseptics on biofilms of bacteria, the method of two successive dilutions was used. The results of a comparative study of the activity of antiseptic agents (decamethoxin, bichluconate chlorhexidine, povidone iodine, hydrogen peroxide) in relation to planktonic and biofilm forms of pseudomonad and acinetobacter strains testify that in all investigated antiseptics and bacterial films pseudomonads forms are more resistant to drags preparations than in planktonic form. In bichluconate, chlorhexidine and decamethoxin MBcK for a bacterial film are 3,2 times greater than for a planktonic form. The smallest difference MBtsK for planktonic and biofilm forms of pseudomonad was in the drug povidone-iodine. MBcC bichluconate chlorhexidine for the biofilm form of acinetobacter, in comparison with planktonic form, was 3,3 times higher, for decamethoxin in 3.7 times, for povidone-iodine in 3,5 times. Biopharmaceutical forms of gram-negative nonfermenting bacteria have the ability to achieve high levels of resistance to antiseptics.

scholarly journals Cross-Contamination by Disinfectant Towelettes Varies by Product Chemistry and Strain

2020 ◽  
Author(s):  
Maxwell Voorn ◽  
Summer Goss ◽  
Carine Nkemngong ◽  
Xiaobao Li ◽  
Peter Teska ◽  
...  
Keyword(s):  
Medical Equipment ◽  
Bacterial Cells ◽  
Cross Contamination ◽  
Strain Atcc ◽  
Healthcare Facilities ◽  
Surface Areas ◽  
Environmental Surfaces ◽  
Viable Bacteria ◽  
High Level ◽  
Product Chemistry

Abstract Background: Disinfectant products are used frequently on environmental surfaces (e.g. medical equipment, countertops, patient beds) and patient care equipment within healthcare facilities. The purpose of this study was to assess the risk of cross-contamination of Staphylococcus aureus and Pseudomonas aeruginosa during and after disinfection of predetermined surface areas with ready-to-use (RTU) pre-wetted disinfectant towelettes.Methods: This study tested six disinfectant towelette products against S. aureus ATCC CRM-6538 and P. aeruginosa strain ATCC-15442 on Formica surfaces. Each disinfectant was evaluated on a hard nonporous surface and efficacy was measured every 0.5 m 2 using a modified version of EPA MLB SOP-MB-33 to study the risk of cross-contamination.Results: We found that all of the wipes used in this study transferred P. aeruginosa and S. aureus from an inoculated surface to previously uncontaminated surfaces. Disinfectant towelettes with certain chemistries also retained a high level of viable bacteria after disinfection of the surface area. The cross-contamination risk also varied by product chemistry and bacterial strain.Conclusion: Disinfectant wipes can cross-contaminate hard nonporous surfaces and retain viable bacterial cells post-disinfection, especially over larger surface areas. This highlights a need to further investigate the risk disinfectant wipes pose during and post-disinfection and guidance on maximum surface areas treated with a single towelette.

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