Presence of a togavirus-like agent in cell cultures from specific pathogen free chick embryos

1986 ◽  
Vol 119 (10) ◽  
pp. 243-244 ◽  
Author(s):  
H. Farmer ◽  
J. Frazier
Keyword(s):  
Cell Cultures ◽  
Chick Embryos ◽  
Specific Pathogen Free ◽  
Specific Pathogen

Infectious Pancreatic Necrosis: Selection of Virus-Free Stock from a Population of Carrier Trout

1968 ◽  
Vol 25 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Ken Wolf ◽  
M. C. Quimby ◽  
C. P. Carlson ◽  
G. L. Bullock
Keyword(s):  
Cell Cultures ◽  
Pancreatic Necrosis ◽  
Control Measure ◽  
Effective Control ◽  
Fish Cell ◽  
Specific Pathogen Free ◽  
Trout Population ◽  
Specific Pathogen ◽  
Infectious Pancreatic Necrosis ◽  
Selection Of

Infectious pancreatic necrosis (IPN) is a virulent disease of young trouts and is easily transmitted from infected animals through water and with eggs. At present, the most effective control measure consists of propagation of specific pathogen-free stock. Methods are described for using fish cell cultures to detect IPN virus in peritoneal washes, feces, and gonadal fluids and thereby to identifiy carrier and non-carrier fish. The procedures were used to derive virus-free progeny from a hatchery trout population containing about one-third carrier fish. The progeny were reared to maturity, spawned for 3 successive years, and at each spawning they proved free of virus. Recommendations and precautions are given for those who may wish to apply the procedures.

Effects of bisphenol A on the development of central immune organs of specific-pathogen-free chick embryos

2012 ◽  
Vol 30 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Jijing Tian ◽  
Dongmei Luo ◽  
Ruiping She ◽  
Tianlong Liu ◽  
Ye Ding ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Chick Embryos ◽  
Specific Pathogen Free ◽  
Immune Organs ◽  
Specific Pathogen

scholarly journals Responses of isolator-derived Japanese quail and quail cell cultures to selected animal viruses

1975 ◽  
Vol 2 (5) ◽  
pp. 419-424
Author(s):  
W M Farrow ◽  
M W Schmitt ◽  
V Groupé
Keyword(s):  
Cell Cultures ◽  
Japanese Quail ◽  
Neutralizing Antibody ◽  
Avian Leukosis Virus ◽  
Embryo Cell ◽  
Marek's Disease ◽  
Marek’S Disease ◽  
Specific Pathogen Free ◽  
Specific Pathogen ◽  
Marek's Disease Herpesvirus

Thirteen oncogenic and necrotizing animal viruses were assayed in LIFE Sciences, Inc. (LSI)-specific pathogen-free Japanese quail and LSI-specific pathogen-free chicken embryo cell cultures. Nine viruses produced similar titers in the quail and chicken cell systems, whereas four viruses showed significantly higher titers in chickens. Young Japanese quail and chickens were inoculated with five selected avain viruses and maintained in stainless-steel isolators. Comparable responses were noted in quail and chickens injected with Newcastle disease virus and avain leukosis virus, but quail were significantly more resistant than chickens to fowl pox virus, laryngotracheitis virus, and Marek's disease herpesvirus. Although no overt symptoms of disease were observed in Japanese quail inoculated with most avain viruses, neutralizing antibody or virus was detected, indicating presence of an inapparent infection. In one experiment, neutralizing antibody was detected in a comparable number of quail and chickens after inoculation with avian leukosis virus. Avian leukosis virus viremia was observed at 12 and 70 days postinoculation, with the COFAL (complement fixation for avian leukosis) titers similar for quail and chickens. Most quail infected with Marek's disease herpesvirus produced neutralizing antibody within 70 days but showed no classical symptoms of Marek's disease even when held for 5 months. In contrast, all chickens inoculated with Marek's disease herpesvirus died within 20 days. The utility of quail embryo cell cultured in the preparation of vaccines and biological reagents is discussed.

Laboratory Animal Research Centre (Larc) “A Specific Pathogen Free Rodent Facility” - Covid – 19 Pandemic Response Plan

2020 ◽  
Author(s):  
Muralitharan Shanmugakonar ◽  
Vijay Kanth Govindharajan ◽  
Kavitha Varadharajan ◽  
Hamda Al-Naemi
Keyword(s):  
Laboratory Animal ◽  
Animal Research ◽  
Research Centre ◽  
Functional Requirement ◽  
Specific Pathogen Free ◽  
Specific Pathogen ◽  
Response Plan ◽  
Pandemic Response ◽  
Operational Plan

Laboratory Animal Research Centre (LARC) has developed an early emergency operational plan for COVID-19 pandemic situation. Biosafety and biosecurity measures were planned and implemented ahead of time to check the functional requirement to prevent the infection. Identified necessary support for IT, transport, procurement, finance, admin and research to make the operations remotely and successfully.

scholarly journals Curcumin reduces enteric isoprostane 8-iso-PGF2α and prostaglandin GF2α in specific pathogen-free Leghorn chickens challenged with Eimeria maxima

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Victor M. Petrone-Garcia ◽  
Raquel Lopez-Arellano ◽  
Gabriela Rodríguez Patiño ◽  
Miriam Aide Castillo Rodríguez ◽  
Daniel Hernandez-Patlan ◽  
...  
Keyword(s):  
Pilot Study ◽  
Broiler Chickens ◽  
Solid Phase ◽  
Oxidation Products ◽  
Specific Pathogen Free ◽  
Post Challenge ◽  
Eimeria Maxima ◽  
Lipid Oxidation Products ◽  
Challenge Control ◽  
Specific Pathogen

AbstractThe purpose of this pilot study was to evaluate and determine the concentration of prostaglandin GF2α (PGF2α) and isoprostane 8‐iso‐PGF2α in plasma and intestine of specific pathogen-free (SPF) Leghorn chickens challenged with Eimeria maxima, with or without dietary supplementation of curcumin using solid‐phase microextraction and ultra‐performance liquid chromatography/tandem mass spectrometry. Eighty 1-day-old male SPF chickens were randomly allocated to one of four groups with four replicates (n = 5 chickens/replicate). Groups consisted of: (1) Control (no challenge), (2) Curcumin (no challenge), (3) Eimeria maxima (challenge), and (4) Eimeria maxima (challenge) + curcumin. At day 28 of age, all chickens in the challenge groups were orally gavaged with 40,000 sporulated E. maxima oocysts. No significant differences (P > 0.05) were observed in the groups regardless of the treatment or challenge with E. maxima. Enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days and 9 days post-challenge were significantly increased (P < 0.01) compared to the non-challenge control chickens. Interestingly, the enteric levels of both isoprostane 8‐iso‐PGF2α and PGF2α at 7 days post-challenge were significantly reduced in chickens fed curcumin, compared to control chickens challenge with E. maxima. At 9 days post-challenge, only levels of isoprostane 8‐iso‐PGF2α in the enteric samples were significantly reduced in chickens challenged with E. maxima supplemented with curcumin, compared with E. maxima challenge chickens. No differences of isoprostane 8‐iso‐PGF2α or PGF2α were observed in plasma at both days of evaluation. Similarly, no significant differences were observed between the challenge control or chickens challenge with E. maxima and supplemented with curcumin at both times of evaluation. The results of this pilot study suggests that the antioxidant anti-inflammatory properties of curcumin reduced the oxidative damage and subsequent intestinal mucosal over-production of lipid oxidation products. Further studies to confirm and extend these results in broiler chickens are required.

scholarly journals Attenuated fibrosis in specific pathogen‐free microbiota in experimental cholestasis‐ and toxin‐induced liver injury

2019 ◽  
Vol 33 (11) ◽  
pp. 12464-12476 ◽  
Author(s):  
Sheida Moghadamrad ◽  
Mohsin Hassan ◽  
Kathy D. McCoy ◽  
Jorum Kirundi ◽  
Philipp Kellmann ◽  
...  
Keyword(s):  
Liver Injury ◽  
Specific Pathogen Free ◽  
Specific Pathogen ◽  
Experimental Cholestasis

Effect of In Ovo Administered Reovirus Vaccines on Immune Responses of Specific-Pathogen-Free Chickens

10.1637/7087 ◽  
2004 ◽  
Vol 48 (1) ◽  
pp. 224-228 ◽  
Author(s):  
Z. Y. Guo ◽  
J. J. Giambrone ◽  
Z. Liu ◽  
T. V. Dormitorio ◽  
Hongzhuan Wu
Keyword(s):  
Immune Responses ◽  
Specific Pathogen Free ◽  
In Ovo ◽  
Specific Pathogen

scholarly journals Characteristic Intestinal Microflora of Specific Pathogen-Free Mice Bred in Two Different Colonies and their Influence on Postnatal Murine Immunocyte Profiles

2005 ◽  
Vol 54 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Taizo NAGURA ◽  
Satoshi HACHIMURA ◽  
Shuichi KAMINOGAWA ◽  
Tsutomu ARITSUKA ◽  
Kikuji ITOH
Keyword(s):  
Intestinal Microflora ◽  
Specific Pathogen Free ◽  
Specific Pathogen
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