Polyphosphate Treatment of Frozen Cod. 2. Effect on Drip, Yield, Lipid Hydrolysis and Protein Extractability in Twice-Frozen Newfoundland Summer Trap and Fall Cod

1964 ◽  
Vol 21 (3) ◽  
pp. 539-548 ◽  
Author(s):  
W. A. MacCallum ◽  
H. S. Shieh ◽  
Dorothy A. Chalker ◽  
W. J. Dyer ◽  
D. R. Idler

Thaw-drip from once-frozen fall-caught Newfoundland cod (Gadus morhua L.), thawed after storage at −23 °C for up to [Formula: see text] weeks, was equal to total drip from twice-frozen fish treated with sodium tripolyphosphate between freezings. Treatment of twice-frozen cod before the first freezing only had no effect on reducing total thaw-drip below that from twice-frozen untreated samples and the yield, although higher in the former than in the latter, was no better than that from once-frozen untreated fillets. Yields from twice-frozen fish were improved greatly by dipping prior to the second freezing and by dipping before each freezing. Treatment between freezings gave yields close to 100% of initial fillet weight whereas losses with the untreated once- and twice-frozen product appear close to 7 and 15%, respectively.With summer-caught trap fish, characterized by higher thaw-drip values which were not affected by treatment, yields of twice-frozen cod were improved; however, losses were still about 12% in fish treated between freezings.Treatment of twice-frozen cod from the two sources did not appreciably affect lipid hydrolysis or protein denaturation. However, lipid content and free fatty acid production were significantly higher in the trap fish samples as compared to the fall-caught cod.

1968 ◽  
Vol 25 (10) ◽  
pp. 2059-2069 ◽  
Author(s):  
Margaret L. Anderson ◽  
Elinor M. Ravesi

Protein extractability decreased as free fatty acid (FFA) was produced in cod muscle aged in ice. The decrease was small compared with that occurring in frozen-stored muscle of similar FFA content. Prolonged extraction in neutral salt solution in the presence and absence of bovine serum albumin (BSA) showed that loss in protein extractability in muscle aged in ice was reversible through dissociation of inextractable material and that the presence of BSA, a FFA acceptor, favored greater dissociation. Ultracentrifugal patterns of protein extracted from ageing muscle showed increasing polydispersity. Phase contrast microscopy showed that the inextractable material contained muscle fragments consisting of bundles of myofibrils, some of full fiber width. These results indicate that in ageing muscle, interaction of contractile protein with FFA results in the formation of a cross-linking network within the muscle fiber causing resistance to fragmentation and to protein extractability, and that the observed smaller loss during ageing in ice is in part due to dissociation occurring during extraction. They suggest that in muscle aged in the frozen state, the reaction between contractile protein and FFA increases and the complexes formed are stabilized.


1968 ◽  
Vol 25 (4) ◽  
pp. 733-755 ◽  
Author(s):  
W. A. MacCallum ◽  
June I. Jaffray ◽  
D. N. Churchill ◽  
D. R. Idler

Quality assessments, based on organoleptic evaluations and chemical tests, were conducted before and after cold storage, on trap-caught cod (Gadus morhua) which was unfrozen, once-frozen, or twice-frozen. The cod was processed both before and during rigor, after icing or after handling without ice, at various times during the catching season.Lactate determinations and pH measurements on whole fish at killing showed a seasonal variation. There was a correlation between lactate determinations and pH measurements in freshly killed and freshly frozen fillets that had been frozen both before and during rigor. Icing immediately after killing slowed the rate of lactate accumulation.Differences in the state of pre- and in-rigor chilled fish at freezing, as assessed by muscle glycogen, lactate, and reserves of ATP, resulted in differences in texture, thaw-drip, and pH. Patterns in texture may be predicted by the amounts of thaw-drip and the pH measured in the cold-stored pack.Intensity of mealiness and short-grained features in freshly frozen (once) fish was correlated with the level of ATP immediately before cooking. Fillets frozen before rigor toughened very slowly in storage at −23 C and the mealy condition eventually disappeared. Hence, fish downgraded for mealiness at freezing improved or maintained initial overall texture scores during 6 months in storage. Although the panel showed a clear preference for fish frozen pre-rigor, it would be impossible for industry to freeze more than a portion of the landings pre-rigor. To obtain the next best once-frozen product, chilled in-rigor fish should be processed.Double freezing gave the next best product from chilled, prerigor fish. Fish frozen pre-rigor in the "dressed" condition and stored at −23 C, then thawed, processed, and refrozen at 16 weeks, was equal in quality to once-frozen controls. The texture in the twice-frozen material prepared from iced, prerigor frozen fish dropped after a further 10 weeks storage, making it little better than the twice-frozen product of commercial-like handling. A very satisfactory twice-frozen product was obtained also by holding unfilleted blocks of non-iced, gutted fish at −46 C before thawing and refreezing, followed by remaining storage at −23 C. However, such low temperature storage is not within the scope of present commercial operations.With pre- and in-rigor frozen material a correlation was found between thaw-drip and pH, thaw-drip and overall texture (in-rigor freezing only), pH and overall texture values, and between pH and toughness. There was also a correlation between extractable protein nitrogen (EPN) values and overall texture scores of fish frozen in various stages of rigor (encountered during unfrozen storage of upwards of 24 hr before processing) but not between EPN and overall acceptability. With twice-frozen fillets prepared from material also handled unfrozen as above, there was significant correlation between EPN values at tasting and overall texture scores and between EPN and overall acceptability scores.Free fatty acid (FFA) values of the trap fish were high and showed no increase as the result of thawing and refreezing or further storage at −23 C or with pre- and in-rigor freezing. Storage at −46 C resulted in less FFA development. There was significant correlation between EPN and FFA values for June-caught once-frozen fish and between FFA values and strictly taste scores for June and July fish but no correlation between FFA and overall texture scores, or in the case of twice-frozen fish of any particular pre-freezing treatment, between FFA and taste, overall texture, or overall acceptability.


1977 ◽  
Vol 34 (12) ◽  
pp. 2369-2373 ◽  
Author(s):  
Doris Fraser Hiltz ◽  
D. H. North ◽  
Barbara Smith Lall ◽  
R. A. Keith

Refrozen silver hake (Merluccius bilinearis), processed as fillets and minced flesh after thawing of stored round fish that had been frozen within 14 h of capture, underwent rapid deterioration during storage at −18 °C compared with once-frozen control materials from the same lot of fish. The estimated maximum storage life of silver hake refrozen as fillets after 3 and 6 mo storage of the round fish at −25 °C was reduced to about 4.5 and 1 mo, respectively, from 10 mo for once-frozen control fillets. Quality of the refrozen materials immediately after thawing and refreezing was similar to that of the round-frozen fish, except after 6 mo, where some initial deterioration occurred, particularly in minced flesh. Minced flesh was more unstable in frozen storage than fillets. In all once- and twice-frozen materials, formation of dimethylamine occurred concomitantly with decrease in protein extractability. Round-frozen fish underwent no loss in protein extractability during 6 mo storage at −25 °C, but some lipid hydrolysis occurred. These results suggest that the freeze–thaw–refreeze process as applied to silver hake will yield a final product of acceptable quality provided that storage of the round fish does not exceed 3–4 mo and that the refrozen materials are marketed within a month after processing. Key words: silver hake, Merluccius bilinearis, refrozen storage, dimethylamine, minced flesh


2001 ◽  
Vol 3 ◽  
pp. 57 ◽  
Author(s):  
G McClelland ◽  
DJ Martell

Between November 1988 and October 1996, >10,000 fish from the Breton Shelf, Sable Island Bank and the northeastern Gulf of Maine were examined for larval anisakines. Larval sealworm, Pseudoterranova decipiens, occurred in 30 of 39 species surveyed, including 8 new host records, Enchelyopus cimbrius, Lycodes reticulatus, Eumesogrammus praecisus, Lumpenus lumpretaeformis, Lumpenus maculatus, Cryptacanthodes maculatus, Artediellus atlanticus and Triglops murrayi. The parasite was most prevalent and abundant in mature demersal piscivores and benthic consumers. Sealworm densities (nr kg-1 host wt.), however, were greatest in small benthophagous fish including mature E. cimbrius, A. atlanticus, T. murrayi and Aspidophoroides monopterygius, and juvenile Hippoglossoides platessoides. ANOVA revealed that geographical disparities in sealworm prevalence and abundance were highly significant in 14 of 20 species tested, although significant disparities between samples from each of the three areas were evident only in H. platessoides. Almost invariably, infection parameters were greatest in fish from Sable Island Bank. ANOVA also indicated that sealworm prevalence and/or abundance increased significantly in Sable Island Bank populations of Gadus morhua, H. platessoides, and seven other species between 1985-1986 and 1989-1990. Routine examinations, in which host flesh was sliced and candled, proved as efficacious as digestion in warm (35° C) pepsin-HCl for detection of larval sealworm in the flesh of large frozen fish. Procedures employing fresh (iced) samples, digestion at ambient temperature and microscopy are recommended, however, for surveys of small benthic consumers. Many of the sealworm infecting the latter hosts are tiny (2 to 10 mm in length) nematodes, which escape detection by routine inspection, and may not survive in warm pepsin-HCl solution.


Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 21-28
Author(s):  
P.T. Olagbemide ◽  
F.C. Akharaiyi

Fishes are world widely consumed by all categories of works of life because of their richness in protein, readily available and affordable by all. The basic nutrient of protein in fish that is so important in man’s diet also attracted microorganisms for their growth and multiplication. Meanwhile, the association of microorganisms in fishes depend on the environment of culture and their proliferation due to inadequate storage facilities. The aim of this study was focused on the isolation and identification of microorganisms from four frozen fish species sold in the Ado Ekiti metropolis. Frozen fish samples of Scomber scombrus (Atlantic Mackerel), Clupea harengus (Atlantic herring), Urophycis tenuis (White hake or mud hake) and Trachurus trachurus (Atlantic horse mackerel) from two markets in Ado Ekiti were microbiologically analyzed for possible microbial contamination. On the fish samples, the total heterotrophic count (THC) was 3.5×104 – 5.6×104 CFU/g, total coliform count (TCC) was 2.4×104 – 5.1×104 CFU/g, total Salmonella/Shigella count (TSSC) was 1.3×104 – 3.5×104 CFU/g, total Vibrio count (TVC) was 1.1×104 – 2.3×104 CFU/g and total fungal count (TFC) was 1.3×103 – 2.3×103 Spore/g were analyzed by cultural methods. There were variations in microbial loads among the fish species in the surveyed markets. The microorganisms identified with their percentage occurrence were Bacillus cereus (11.54%), Streptococcus faecium (13.46%), Alcaligenes faecalis (5.77%), Salmonella enterica serovar Typhi (5.77%), Micrococcus luteus (9.62%), Vibrio cholerae (7.69%), Aerococcus viridans (3.85%), Pseudomonas aeruginosa (7.69%), Xanthomonas fragariae (7.69%), Staphylococcus aureus (11.54%), Clostridium butyricum (7.68%), Escherichia coli (7.69%), Aspergillus fumigatus (11.11%), Aspergillus flavus (24.44%), Aspergillus clavatus (8.89%), Aspergillus fishcheri (6.69%), Aspergillus terreus (8.89%), Mucor mucedo (17.78%), Penicillium digitatum (13.33%) and Aspergillus parasiticus (8.89%). The results emphasized the microbial contamination of the fishes. The results obtained could serve as an awareness to consumers that microbial infection is possible from frozen fishes and as data for future reference in epidemiology or outbreak of disease from eating frozen fish.


1986 ◽  
Vol 69 (1) ◽  
pp. 75-79
Author(s):  
Johannes Bon ◽  
Kerst K Brünner ◽  
Angus Aitken

Abstract Four collaborative trials are described in which 3 different methods for the estimation of fish content of coated products were compared to determine their suitability. The methods are a modified version of AOAC method 18.003, an alternative “scraping” method, and a “soaking” method. Seven West European laboratories participated in this collaborative exercise, which was performed on 4 types of coated fish products: raw coated portions of cod (Gadus morhua), partly cooked cod sticks, raw fillets of plaice (Pleuronectes platessa), and partly cooked plaice fillets. The results indicate that precooking strongly affected fish recovery by all 3 methods. Regular portions (of cod) gave higher recoveries than irregular shaped fillets (of plaice). The results do not lead to the conclusion that any method was better than the others.


2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Vincenzo Ferrantelli ◽  
Antonella Costa ◽  
Stefania Graci ◽  
Maria Drusilla Buscemi ◽  
Giuseppe Giangrosso ◽  
...  

In this work a total of 949 fish samples were analysed for the identification of nematode larvae belonging to the Anisakidae family. Biomolecular application for the identification of Anisakidae larvae can be an optimal instrument for the traceability of fish products, described on the Reg. EC 178/2002. Results confirm a correlation between geographical distribution of fishes and presence of specific Anisakid larvae. FAO 37 zone (Mediterranean sea) showed a prevailing distribution of <em>Anisakis pegreffii</em> and a minimal presence of A. simplex s.s. in hybrid form with <em>Anisakis pegreffii</em>. FAO 27 zone showed a prevailing distribution of <em>A. simplex s.s</em>. in fish like Brosme (<em>Brosme brosme</em>) and infestation prevalence of Pseudoterranova krabbei and <em>P. decipiens s.s</em>. in <em>Gadus morhua</em>. Obtained results validate the hypothesis that molecular biology methods for identifying Anisakidae larvae are effective traceability markers of fish products.


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