Differential expression of corticosteroid receptor genes in rainbow trout (Oncorhynchus mykiss) immune system in response to acute stress

2007 ◽  
Vol 64 (10) ◽  
pp. 1382-1389 ◽  
Author(s):  
Takashi Yada ◽  
Teruo Azuma ◽  
Susumu Hyodo ◽  
Tetsuya Hirano ◽  
E Gordon Grau ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Plasma Cortisol ◽  
Glucocorticoid Receptors ◽  
Acute Stress ◽  
Mrna Levels ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Corticosteroid Receptor ◽  
Polymerase Chain ◽  
Peripheral Blood Leucocytes

Expression of distinct corticosteroid receptor genes, glucocorticoid receptors 1 and 2 (GR-1 and GR-2, respectively) and mineralcorticoid receptor (MR), was quantified by real-time polymerase chain reaction (PCR) in peripheral blood leucocytes (PBL), spleen, and gill of rainbow trout (Oncorhynchus mykiss) after an acute netting stress. Plasma cortisol levels were significantly increased 2 h after stress and returned to prestress levels within 24 h. Consistent with changes in plasma cortisol, GR-2 mRNA levels in PBL increased significantly at 2 h after stress, returning to initial levels by 8 h. In contrast, GR-1 and MR levels in PBL decreased significantly at 24 h after stress, and these reduced levels were maintained for 7 days. Splenic mRNA levels of GR-1 and GR-2 also decreased at 8 h and 24 h after stress, returning to control levels by 7 days, whereas no significant change was observed in MR. In gill, there was no obvious change in corticosteroid receptor mRNA levels after stress, except for a transient decrease at 8 h in MR. These results suggest a variety of roles for the three corticosteroid receptors during immunosuppression in response to acute stress in trout.

Acute stress alters intestinal function of rainbow trout, Oncorhynchus mykiss (Walbaum)

Aquaculture ◽  
2005 ◽  
Vol 250 (1-2) ◽  
pp. 480-495 ◽  
Author(s):  
Rolf Erik Olsen ◽  
Kristina Sundell ◽  
Terry M. Mayhew ◽  
Reidar Myklebust ◽  
Einar Ringø
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Acute Stress ◽  
Intestinal Function ◽  
Rainbow Trout Oncorhynchus Mykiss

Effects of dietary nucleotides supplementation on rainbow trout (Oncorhynchus mykiss) performance and acute stress response

2011 ◽  
Vol 38 (2) ◽  
pp. 431-440 ◽  
Author(s):  
Ahmad Tahmasebi-Kohyani ◽  
Saeed Keyvanshokooh ◽  
Amin Nematollahi ◽  
Nemat Mahmoudi ◽  
Hossein Pasha-Zanoosi
Keyword(s):  
Rainbow Trout ◽  
Stress Response ◽  
Oncorhynchus Mykiss ◽  
Acute Stress ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dietary Nucleotides ◽  
Acute Stress Response

Individual variation and interrelationships between swimming performance, growth rate, and feeding in juvenile rainbow trout (Oncorhynchus mykiss)

1998 ◽  
Vol 55 (7) ◽  
pp. 1583-1590 ◽  
Author(s):  
T Ryan Gregory ◽  
Chris M Wood
Keyword(s):  
Growth Rate ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Plasma Cortisol ◽  
Positive Relationship ◽  
Swimming Performance ◽  
Individual Performance ◽  
Significant Positive Relationship ◽  
Juvenile Rainbow Trout ◽  
Rainbow Trout Oncorhynchus Mykiss

Variation among individuals in specific growth rate (SGR), feeding, and two measures of swimming performance and their possible interrelationships were investigated in juvenile rainbow trout (Oncorhynchus mykiss) kept in groups on either satiation or half-satiation rations. Maximum sustainable velocity (Ucrit) was measured as an index of aerobic swimming performance and stamina (fatigue time in a fixed-velocity test at 6 body lengths ·s-1) as an index of anaerobic performance. Individual performance in both of these tests was found to be significantly repeatable. Trout fed on half-satiation ration exhibited significantly lower mean values of SGR and body size and higher levels of aggression-related fin damage, but no significant differences in stamina, relative or absolute Ucrit, glycogen content, or plasma cortisol. However, in these fish, there was a significant negative relationship between SGR and relative Ucrit, a significant positive relationship between SGR and stamina, and a significant positive relationship between SGR and total daily meal. None of these relationships were seen in fish fed to satiation. Plasma cortisol and tissue glycogen stores were not related to SGR. These results indicate that under the intensified competition of restricted ration, there are trade-offs between growth, feeding, and different types of swimming performance.

Stream habitat and rainbow trout (Oncorhynchus mykiss) physiological stress responses to streamside clear-cut logging in British Columbia

2005 ◽  
Vol 35 (3) ◽  
pp. 541-556 ◽  
Author(s):  
Eric Mellina ◽  
Scott G Hinch ◽  
Edward M Donaldson ◽  
Greg Pearson
Keyword(s):  
British Columbia ◽  
Rainbow Trout ◽  
Chronic Stress ◽  
Stress Responses ◽  
Plasma Cortisol ◽  
Acute Stress ◽  
Physiological Stress ◽  
Stream Habitat ◽  
Clear Cut ◽  
Rainbow Trout Oncorhynchus Mykiss

The impacts associated with streamside clear-cut logging (e.g., increased temperatures and sedimentation, loss of habitat complexity) are potentially stressful to stream-dwelling fish. We examined stream habitat and rainbow trout physiological stress responses to clear-cut logging in north-central British Columbia using 15 streams divided into three categories: old growth (reference), recently logged (clear-cut to both banks 1–9 years prior to the study), and second growth (clear-cut 25–28 years prior to the study). We used plasma cortisol and chloride concentrations as indicators of acute stress, and interrenal nuclear diameters, impairment of the plasma cortisol response, and trout condition and length-at-age estimates as indicators of chronic stress. No statistically significant acute or chronic stress responses to streamside logging were found, despite increases in summertime stream temperatures (daily maxima and diurnal fluctuations) and a reduction in the average overall availability of pool habitat. Our observed stress responses were approximately an order of magnitude lower than what has previously been reported in the literature for a variety of different stressors, and trout interrenal nuclear diameters responses to the onset of winter were approximately five times greater than those to logging. The overall consistency of our results suggests that the impacts of streamside clear-cut logging are not acutely or chronically stressful to rainbow trout in our study area.

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