Androgen and estrogen treatments alter steady state messengers RNA (mRNA) levels of testicular steroidogenic enzymes in the rainbow trout, Oncorhynchus mykiss

2005 ◽  
Vol 71 (4) ◽  
pp. 471-479 ◽  
Author(s):  
D. Baron ◽  
A. Fostier ◽  
B. Breton ◽  
Y. Guiguen
Keyword(s):  
Steady State ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Mrna Levels ◽  
Steroidogenic Enzymes ◽  
Rainbow Trout Oncorhynchus Mykiss

The effects of cell ageing on protein synthesis in rainbow trout (Oncorhynchus mykiss) red blood cells

2000 ◽  
Vol 203 (14) ◽  
pp. 2219-2228
Author(s):  
S.G. Lund ◽  
M.C. Phillips ◽  
C.D. Moyes ◽  
B.L. Tufts
Keyword(s):  
Protein Synthesis ◽  
Steady State ◽  
Rainbow Trout ◽  
Heat Shock ◽  
Oncorhynchus Mykiss ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Mrna Levels ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Cell Age

The effects of cell age on protein synthesis were examined in the nucleated red blood cells of rainbow trout (Oncorhynchus mykiss). Total DNA content was unaffected by cell age, whereas total RNA content in young red blood cells was roughly ten times as high as that in old red blood cells. The mRNA levels for haemoglobin, carbonic anhydrase and the chloride/bicarbonate (Cl(−)/HCO(3)(−)) exchanger were also approximately tenfold higher in young red blood cells. Although young red blood cells synthesized roughly five times more protein under steady-state conditions, total protein concentration was not affected by cell age. Despite large reductions in mRNA levels with red blood cell ageing, the concentrations and/or activities of the respiratory proteins were largely preserved. In contrast, the ability to mount a heat shock response was greatly reduced in older red blood cells. Young red blood cells produced 13 times more heat shock protein 70 mRNA following heat shock and four times more 70 kDa protein after recovery. They also transcribed much more heat shock cognate 71 and heat shock factor mRNA than did older red blood cells under steady-state conditions.

Differential expression of corticosteroid receptor genes in rainbow trout (Oncorhynchus mykiss) immune system in response to acute stress

2007 ◽  
Vol 64 (10) ◽  
pp. 1382-1389 ◽  
Author(s):  
Takashi Yada ◽  
Teruo Azuma ◽  
Susumu Hyodo ◽  
Tetsuya Hirano ◽  
E Gordon Grau ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Plasma Cortisol ◽  
Glucocorticoid Receptors ◽  
Acute Stress ◽  
Mrna Levels ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Corticosteroid Receptor ◽  
Polymerase Chain ◽  
Peripheral Blood Leucocytes

Expression of distinct corticosteroid receptor genes, glucocorticoid receptors 1 and 2 (GR-1 and GR-2, respectively) and mineralcorticoid receptor (MR), was quantified by real-time polymerase chain reaction (PCR) in peripheral blood leucocytes (PBL), spleen, and gill of rainbow trout (Oncorhynchus mykiss) after an acute netting stress. Plasma cortisol levels were significantly increased 2 h after stress and returned to prestress levels within 24 h. Consistent with changes in plasma cortisol, GR-2 mRNA levels in PBL increased significantly at 2 h after stress, returning to initial levels by 8 h. In contrast, GR-1 and MR levels in PBL decreased significantly at 24 h after stress, and these reduced levels were maintained for 7 days. Splenic mRNA levels of GR-1 and GR-2 also decreased at 8 h and 24 h after stress, returning to control levels by 7 days, whereas no significant change was observed in MR. In gill, there was no obvious change in corticosteroid receptor mRNA levels after stress, except for a transient decrease at 8 h in MR. These results suggest a variety of roles for the three corticosteroid receptors during immunosuppression in response to acute stress in trout.

scholarly journals Molecular cloning and expression analysis of rainbow trout (Oncorhynchus mykiss) CCAAT/enhancer binding protein genes and their responses to induction by GH in vitro and in vivo

2007 ◽  
Vol 194 (2) ◽  
pp. 393-406 ◽  
Author(s):  
Jay H Lo ◽  
Pinwen Peter Chiou ◽  
C M Lin ◽  
Thomas T Chen
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Cloning And Expression ◽  
Mrna Levels ◽  
Specific Expression ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Enhancer Binding Protein ◽  
Previous Hypothesis

CCAAT/enhancer-binding proteins (C/EBPs) are transcription factors consisting of six isoforms and play diverse physiological roles in vertebrates. In rainbow trout (Oncorhynchus mykiss), in addition to the reported C/EBPβ1, we have isolated cDNA of four other isoforms, C/EBPα, C/EBPβ2, C/EBPδ1, C/EBPδ2, from the liver. Comparison of the deduced amino acid sequence of rainbow trout C/EBPs with those of other vertebrates revealed that C/EBP isoforms are highly conserved. The profiles of tissue-specific expression of individual C/EBP isoform mRNA, determined by quantitative real-time (RT)-PCR showed distinct patterns. Furthermore, injection of bovine GH into yearling rainbow trout resulted in a significant increase of mRNA levels of C/EBPβ1, C/EBPβ2, and C/EBPδ2 but not C/EBPα and C/EBPδ1 in the liver. GH-dependent increase of mRNA levels of C/EBPβ1, C/EBPβ2, C/EBPδ2, and IGF-II were also confirmed by treating rainbow trout hepatoma cells expressing a goldfish GH receptor with bGH. Together with our previous findings, the results presented in this paper strengthen our previous hypothesis that GH may regulate the expression of the IGF-II gene via mediating the expression of C/EBPβ1, C/EBPβ2, and C/EBPδ2 mRNA.

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