A CYTOCHEMICAL STUDY OF THE ABSORPTION AND DISTRIBUTION OF IRON IN THE FROG, RANA PIPIENS

1950 ◽  
Vol 28d (3) ◽  
pp. 119-125 ◽  
Author(s):  
David J. McCallion ◽  
Joseph L. Scott
Keyword(s):  
Intestinal Mucosa ◽  
Large Dose ◽  
Metallic Iron ◽  
Rana Pipiens ◽  
Stellate Cells ◽  
Ferric Sulphate ◽  
Maximum Condition ◽  
Liver Iron ◽  
Cytochemical Study ◽  
Free Iron

Cytochemical studies for iron in starved frogs fed a single large dose (50 to 200 mgm.) of ferric sulphate or metallic iron showed that free iron was most readily absorbed by the intestinal mucosa of the duodenum. Liver iron increased slightly two hours after feeding, reached a maximum condition after six hours, and decreased considerably by 12 hr. The increased liver iron was found in the Kuppfer cells, the lining cells of the sinusoids, and the stellate cells of the pigment patches. Iron in the liver of control frogs was usually confined to the pigment patches. After ferric sulphate solution was fed there was an increase in the amount of free iron in the spleen and in the liver but not in the kidney. There was a marked increase in masked iron in the nuclei of the cells of the liver and spleen, and even in those of the kidney of these same animals.

Heparin from Intestine and Lung Source Administered in Large Dose to Dogs with Acute Myocardial Ischemia

1978 ◽  
Vol 40 (02) ◽  
pp. 407-417
Author(s):  
Michael J Saliba ◽  
Richard J Pavalec
Keyword(s):  
Coronary Artery ◽  
Myocardial Ischemia ◽  
Adenosine Triphosphate ◽  
Intestinal Mucosa ◽  
Large Dose ◽  
Acute Myocardial Ischemia ◽  
Ischemic Myocardium ◽  
Atp Levels ◽  
Initial Control ◽  
The Difference

SummaryIntestinal mucosa heparin (IMH) and beef lung heparin (BLH) were infused into dogs subjected to myocardial ischemia by intermittent coronary artery occlusions. The IMH was from a mixture of beef, sheep, and pig intestinal mucosa. Initial control occlusion and recovery was followed by a second occlusion with 60,000 units of IMH or BLH added. Electrocardiographic S-T segment elevations (ST) were measured acutely. There were no significant differences in ST in non-ischemic myocardium before occlusions or with occlusions. In ischemic myocardium, IMH significantly lowered control ST 84% in amount (t = 6.1 p <0.00005), and 76% in number (t = 11.6 p <0.00001). BLH lowered control ST a significant, lesser, 36% in amount (t = 3.6 p <0.008), and 35% in number (t = 3.2 p <0.01). The difference between IMH and BLH in ischemic myocardium was a significant 48% in amount (t = 4.0 p <0.0007), and 41% in number (t = 2.0 p <0.06). Myocardial adenosine triphosphate (ATP) levels were assayed after 90 min. ATP levels were 31% higher in both ischemic and non-ischemic myocardium in IMH-treated dogs than in BLH- treated. It was concluded that IMH and BLH are functionally different, and IMH was significantly more effective.

Importance of Anemia and Transferrin Levels in the Regulation of Intestinal Iron Absorption in Hypotransferrinemic Mice

Blood ◽  
1999 ◽  
Vol 94 (9) ◽  
pp. 3185-3192 ◽  
Author(s):  
K.B. Raja ◽  
D.J. Pountney ◽  
R.J. Simpson ◽  
T.J. Peters
Keyword(s):  
Mutant Strain ◽  
Intestinal Mucosa ◽  
Iron Uptake ◽  
Iron Absorption ◽  
Total Iron ◽  
Hepatic Iron ◽  
Short Term ◽  
Liver Iron ◽  
Intestinal Iron Absorption ◽  
Per Se

The hypotransferrinemic mouse (trf hpx) is a mutant strain exhibiting transferrin deficiency, marked anemia, hyperabsorption of iron, and elevated hepatic iron stores. We set out to investigate the relative roles of anemia and of transferrin in the malregulation of intestinal iron absorption in these animals. Transfusion of erythrocytes obtained from littermate controls increased hemoglobin levels and reduced reticulocyte counts in recipient animals. Although mucosal to carcass 59Fe transfer was reduced, total duodenal iron uptake was not significantly affected. Iron absorption in homozygotes, in contrast to littermate controls, was not reduced by hyperoxia. Mouse transferrin injections, in the short term, increased delivery of iron to the marrow and raised hemoglobin levels. Although mucosal transfer and total iron uptake were reduced at the higher transferrin doses, total uptake was still higher than in controls. Daily injections of mouse/human transferrin for 3 weeks from weaning, normalized hemoglobin values, and markedly reduced liver iron and intestinal iron absorption values in trf hpxanimals. When such daily-injected mice were left for a week to allow transferrin clearance, iron absorption values were significantly enhanced; hemoglobin or hepatic iron levels were, however, not significantly altered. These data indicate that hyperabsorption of iron in trf hpx mice is not solely because of the anemia; transferrin levels per se do affect iron absorption, possibly via a direct effect on the intestinal mucosa.

Effect of free iron on collagen synthesis, cell proliferation and MMP-2 expression in rat hepatic stellate cells

2002 ◽  
Vol 64 (7) ◽  
pp. 1139-1145 ◽  
Author(s):  
Concetta Gardi ◽  
Beatrice Arezzini ◽  
Vittoria Fortino ◽  
Mario Comporti
Keyword(s):  
Cell Proliferation ◽  
Hepatic Stellate Cells ◽  
Collagen Synthesis ◽  
Stellate Cells ◽  
Free Iron

Importance of Anemia and Transferrin Levels in the Regulation of Intestinal Iron Absorption in Hypotransferrinemic Mice

Blood ◽  
1999 ◽  
Vol 94 (9) ◽  
pp. 3185-3192 ◽  
Author(s):  
K.B. Raja ◽  
D.J. Pountney ◽  
R.J. Simpson ◽  
T.J. Peters
Keyword(s):  
Mutant Strain ◽  
Intestinal Mucosa ◽  
Iron Uptake ◽  
Iron Absorption ◽  
Total Iron ◽  
Hepatic Iron ◽  
Short Term ◽  
Liver Iron ◽  
Intestinal Iron Absorption ◽  
Per Se

Abstract The hypotransferrinemic mouse (trf hpx) is a mutant strain exhibiting transferrin deficiency, marked anemia, hyperabsorption of iron, and elevated hepatic iron stores. We set out to investigate the relative roles of anemia and of transferrin in the malregulation of intestinal iron absorption in these animals. Transfusion of erythrocytes obtained from littermate controls increased hemoglobin levels and reduced reticulocyte counts in recipient animals. Although mucosal to carcass 59Fe transfer was reduced, total duodenal iron uptake was not significantly affected. Iron absorption in homozygotes, in contrast to littermate controls, was not reduced by hyperoxia. Mouse transferrin injections, in the short term, increased delivery of iron to the marrow and raised hemoglobin levels. Although mucosal transfer and total iron uptake were reduced at the higher transferrin doses, total uptake was still higher than in controls. Daily injections of mouse/human transferrin for 3 weeks from weaning, normalized hemoglobin values, and markedly reduced liver iron and intestinal iron absorption values in trf hpxanimals. When such daily-injected mice were left for a week to allow transferrin clearance, iron absorption values were significantly enhanced; hemoglobin or hepatic iron levels were, however, not significantly altered. These data indicate that hyperabsorption of iron in trf hpx mice is not solely because of the anemia; transferrin levels per se do affect iron absorption, possibly via a direct effect on the intestinal mucosa.

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