METHODS FOR THE DETERMINATION OF THE DISTRIBUTION OF RADIOACTIVE PHOSPHORUS AMONG THE PHOSPHORUS-CONTAINING CONSTITUENTS OF TISSUES

1948 ◽  
Vol 26e (6) ◽  
pp. 333-346 ◽  
Author(s):  
J. A. McCarter ◽  
Ethel L. Steljes
Keyword(s):  
Rat Liver ◽  
Inorganic Phosphate ◽  
Trichloroacetic Acid ◽  
Organic Phosphorus ◽  
Prostate Gland ◽  
Phosphorus Fraction ◽  
Radioactive Phosphorus ◽  
Phosphorus Containing ◽  
Quantitative Account

The phosphorus-containing constituents of various tissues of the rat were separated into five fractions. These were: inorganic and organic phosphorus soluble in aqueous 10% trichloroacetic acid; phosphorus soluble in 3:1 alcohol–ether; phosphorus derived from ribonucleic acid; and phosphorus derived from thymonucleic acid. In order to test the methods, inorganic phosphate, disodium phenyl phosphate, and purified ribonucleic and thymonucleic acids were added to a homogenate of rat liver in 10% trichloroacetic acid. It was possible by separation and analysis of the fractions to obtain a quantitative recovery of the added phosphorus in the appropriate fractions. The extent to which radioactive inorganic phosphate entered the other fractions was measured following its addition to a homogenate of rat liver in 10% trichloroacetic acid. When the fractionation was performed at room temperature it was found that all of the fractions except the organic acid-soluble phosphorus fraction contained negligible amounts of the added radioactive phosphorus. The amount of radioactive phosphorus taken up by this fraction could be reduced to a negligible quantity by working quickly and with cold solutions.Data are given showing the radioactive and nonradioactive phosphorus contents of the various fractions of liver, spleen, kidney, muscle, thymus gland, lymph node, prostate gland, and testis of the rat 24 hr. after the intraperitoneal injection of a solution of radioactive inorganic phosphate. By analysis of the urine, feces, and remainder of the rat after removal of the above tissues, a quantitative account was made of the injected radioactive phosphate.

THE FRACTIONATION OF PHOSPHORUS CONTAINING CONSTITUENTS IN THE ALLANTOIC MEMBRANE OF THE EMBRYONATED EGG

1950 ◽  
Vol 28e (6) ◽  
pp. 271-280 ◽  
Author(s):  
A. F. Graham
Keyword(s):  
Influenza Virus ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Total Phosphorus ◽  
Trichloroacetic Acid ◽  
Inorganic Phosphorus ◽  
Radioactive Phosphorus ◽  
Significant Difference ◽  
Phosphorus Containing ◽  
Soluble Phosphorus

A procedure is described which permitted the phosphorus containing constituents in allantoic membranes of embryonated eggs to be separated into fractions as follows: alcohol soluble phosphorus from which the phospholipids were separated, phosphorus soluble in 5% trichloroacetic acid in which inorganic phosphorus was determined, and nucleic acid phosphorus which was further separated into pentose and desoxypentose nucleic acids. This procedure was applied to membranes between 9 and 13 days of age. It was found that the total phosphorus amounted to 9 to 10 mgm. per gm. of dried tissue. Alcohol soluble phosphorus accounted for approximately 28%, acid soluble phosphorus for 37%, and nucleic acid phosphorus for 35% of the total phosphorus. About half the acid soluble phosphorus was inorganic and about 85% of the alcohol soluble phosphorus was associated with phospholipids. These proportions remained essentially constant over the period studied. The ratio of pentose to desoxypentose nucleic acid phosphorus also remained fairly constant over the interval at about 2.2. There was no significant difference in the amounts of P32 taken up over a period of 72 hr. by normal allantoic membranes and those infected with influenza virus, when inorganic radioactive phosphorus was placed in the allantoic sacs of 11-day embryonated eggs.

scholarly journals Determination of Inorganic Phosphate in Rat Liver.

1972 ◽  
Vol 26 ◽  
pp. 1166-1170 ◽  
Author(s):  
S. E. Hansen ◽  
H. I. D. Thieden ◽  
J. Dich ◽  
Karin Nilsson ◽  
Wolfgang Nimmich
Keyword(s):  
Rat Liver ◽  
Inorganic Phosphate

DETERMINATION OF THYROTROPHIN BY MEANS OF RADIOACTIVE PHOSPHORUS

1949 ◽  
Vol 3 (4) ◽  
pp. 331-341 ◽  
Author(s):  
ULF BORELL ◽  
HJALMAR HOLMGREN
Keyword(s):  
Radioactive Phosphorus

Determination of circulating red blood cell volume with radioactive phosphorus

1949 ◽  
Vol 7 (2) ◽  
pp. 259
Author(s):  
R.T. Nieset ◽  
Blanche Porter ◽  
W.S. Trautman ◽  
Ralph M. Bell ◽  
William Parson ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Radioactive Phosphorus

Glucocorticoid effects on respiration and metabolism by rat liver homogenates

1962 ◽  
Vol 202 (2) ◽  
pp. 343-346 ◽  
Author(s):  
Dennis D. Goetsch ◽  
L. E. McDonald
Keyword(s):  
Lactic Acid ◽  
Oxygen Uptake ◽  
Rat Liver ◽  
Inorganic Phosphate ◽  
Chronic Administration ◽  
Glucocorticoid Treatment ◽  
Carbohydrate Utilization ◽  
Protein Levels ◽  
Liver Homogenates ◽  
And Control

The effects of glucocorticoid administration on oxygen uptake, glucose and glycogen disappearance, lactic acid formation, and inorganic phosphate and protein levels in rat liver homogenates have been studied. A single injection of hydrocortisone, prednisolone, or 9 α-fluoroprednisolone 5 hr before sacrifice resulted in a highly significant increase in oxygen uptake by rat liver homogenates, whereas chronic administration of prednisolone daily for 7 days caused a marked inhibition in homogenate respiration. Glycolytic rate did not appear to be affected by single injections since endogenous carbohydrate utilization was similar in liver homogenates prepared from control and treated animals. Incubation of liver homogenates under aerobic conditions disclosed that inorganic phosphate levels were decreased in homogenates from corticoid-treated rats, whereas these levels were similar in treated and control liver homogenates incubated under nitrogen. Under anaerobic conditions, liver homogenates from treated rats accumulated lactic acid more rapidly than untreated liver homogenates. Glucocorticoid treatment did not appear to affect protein disappearance since no differences between protein levels in treated and untreated rat liver homogenates were detected following incubation.

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