A CONTRIBUTION TO THE STUDY OF DORMANCY IN SEEDS: LACTUCA SATIVA L.
The ease with which lettuce seeds may be made dormant by placing them under germinating conditions in blue light suggested that this kind of seed would provide satisfactory material for a study of certain aspects of dormancy in seeds. Studies have been made of the behavior of lettuce seeds under varied conditions of moisture, atmosphere, color of light, and integrity of the testa. Measurements of the absolute respiration have provided curves of the drift with time of carbon dioxide output, oxygen uptake, and of respiratory quotient. Experiments with substances that might induce dormancy and light-sensitiveness similar to that induced by blue light have been made. The bearing of the results obtained upon possible metabolic changes in the seed are discussed.The possibility that the observed effects of light upon seed germination are due to changes in the permeability of the seed coat induced by light is considered as untenable, but this does not exclude the fact that the seed coat is a restrictive agency to the free passage of oxygen, carbon dioxide, and other substances. Indeed, in any description of the probable metabolic state, this must certainly be considered. While it has not been possible to formulate a definite biochemical scheme to account for blue light effects, the experimental evidence suggests that 'carbon dioxide zymasis' may be promoted by blue light and that carbon dioxide may be one of the inhibiting factors. The blue light dormant condition is characterized by depressed respiration but without more disturbance of the equilibrium of the pre- and postglycolytic phases of respiratory metabolism than is found to be characteristic of uninhibited seeds as reported by other workers.The results of this research suggest that further elucidation of the phenomenon of dormancy should be sought in the direction of more extended studies of the respiration of dormant and non-dormant seeds.