PRODUCTION AND PROPERTIES OF 2,3-BUTANEDIOL: XVIII. DISSIMILATION OF GLUCOSE BY SERRATIA MARCESCENS

1947 ◽  
Vol 25b (1) ◽  
pp. 65-69 ◽  
Author(s):  
A. C. Neish ◽  
F. M. Robertson ◽  
A. C. Blackwood ◽  
G. A. Ledingham
Keyword(s):  
Lactic Acid ◽  
Formic Acid ◽  
Serratia Marcescens ◽  
Anaerobic Conditions ◽  
Aerobic Conditions

Four strains of Serratia marcescens were found to dissimilate glucose under anaerobic conditions, producing 2,3-butanediol according to the equation: C6H12O6 → CH3∙CHOH∙CHOH∙CH3 + HCOOH + CO2. Under aerobic conditions little formic acid was produced and the reaction became [Formula: see text]. These reactions accounted for 40 to 60% of the glucose. The remainder was fermented to lactic acid (5 to 25%), ethanol (10 to 20%) and glycerol (1 to 4%). The lactic acid produced was 95 to 97% of the levo-isomer; the 2,3-butanediol was a meso-dextro mixture containing about 2% of the dextro-isomer.

DISSIMILATION OF GLUCOSE BY BACILLUS SUBTILIS (FORD'S STRAIN)

1945 ◽  
Vol 23b (6) ◽  
pp. 290-296 ◽  
Author(s):  
A. C. Neish ◽  
A. C. Blackwood ◽  
G. A. Ledingham
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Formic Acid ◽  
Alkaline Media ◽  
Anaerobic Conditions ◽  
Aerobic Conditions

Ford's strain of Bacillus subtilis (N.C.T.C. 2586) dissimilated glucose mainly to 2,3-butanediol and glycerol under anaerobic conditions at pH 6.2 to 6.8. For each 100 moles of glucose fermented, 57 moles of 2,3-butanediol, 40 moles of glycerol, 20 moles of lactic acid, 13 moles of ethanol, and 5 moles of formic acid were produced. Aerobic conditions favoured formation of 2,3-butanediol and acetoin, oxidation of the substrate, and formation of acetic and butyric acids, but greatly depressed the amount of glycerol and lactic acid formed. In alkaline media (pH 7.5), acids were formed at the expense of the diol and glycerol.

PRODUCTION AND PROPERTIES OF 2,3-BUTANEDIOL: XXV. DISSIMILATION OF GLUCOSE BY BACTERIA OF THE GENUS SERRATIA

1948 ◽  
Vol 26b (3) ◽  
pp. 335-342 ◽  
Author(s):  
A. C. Neish ◽  
A. C. Blackwood ◽  
Florence M. Robertson ◽  
G. A. Ledingham
Keyword(s):  
Carbon Dioxide ◽  
Lactic Acid ◽  
Formic Acid ◽  
Succinic Acid ◽  
Anaerobic Conditions ◽  
Hydrogen Formation ◽  
Aerobic Conditions ◽  
The Third ◽  
Typical Strain ◽  
Different Strains

The genus Serratia may be divided into three groups on the basis of three characteristic fermentations found under anaerobic conditions. The first group, comprised of all strains of S. marcescens, S. anolium, and S. indica tested and one strain named S. kielensis, dissimilates glucose as follows: C6H12O6 → CH3CHOHCHOHCH3 + HCOOH + CO2. The second group, containing S. plymouthensis and some unnamed strains, dissimilates glucose according to the equation: C6H12O6 → CH3CHOHCHOHCH3 + 2CO2 + H2. The third group containing only the most typical strain of S. kielensis carries out the reaction: C6H12O6 + 2H2O → 2CH3COOH + 2CO2 + 4H2. These reactions account for approximately one-half of the glucose utilized, the remainder being accounted for chiefly by the ethanol and lactic acid fermentations which are found in varying proportions with different strains. All strains form some succinic acid, probably by carbon dioxide fixation. Under aerobic conditions carbon dioxide formation is stimulated, chiefly at the expense of formic acid with organisms of the first group, while hydrogen formation by organisms of the second and third groups is depressed.

The metabolism of glucose and pyruvate in rat retina

1962 ◽  
Vol 156 (963) ◽  
pp. 139-143 ◽  
Keyword(s):  
Carbon Dioxide ◽  
Lactic Acid ◽  
Quantitative Data ◽  
Lactate Production ◽  
Anaerobic Conditions ◽  
Aerobic Conditions ◽  
Rat Retina ◽  
Adult Rat ◽  
Total Glucose

The metabolism of [U- 14 C]glucose and [3- 14 C]pyruvate in the adult rat retina is described. In vitro under aerobic conditions, in either phosphate or bicarbonate medium, glucose was converted into lactate, carbon dioxide, glutamate, γ -aminobutyrate, aspartate, glutamine and alanine. Under anaerobic conditions, total glucose metabolized was reduced to 60 to 70% of that under aerobic conditions, lactic acid being the only metabolic product detected. Under aerobic conditions [3- 14 C]pyruvate was converted by the retina into the same metabolites as was glucose. The quantitative data for oxygen uptake and 14 CO 2 formation were similar to those obtained with glucose as substrate; lactate production was lower and amino acid formation higher.

THE FERMENTATION OF D-ALLOSE AND D-GLUCOSE BY AEROBACTER AEROGENES

1955 ◽  
Vol 1 (7) ◽  
pp. 473-478 ◽  
Author(s):  
H. A. Altermatt ◽  
F. J. Simpson ◽  
A. C. Neish
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Methyl Groups ◽  
Carboxyl Group ◽  
Hexose Monophosphate ◽  
Anaerobic Conditions ◽  
Aerobacter Aerogenes ◽  
Aerobic Conditions ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Aerobacter aerogenes rapidly ferments D-allose-1-C14 and D-alIose-2-C14 under aerobic and anaerobic conditions to give products labelled in the same manner as those obtained from similar fermentations of D-glucose-1-C14 and D-glucose-2-C14. The lactic acid, acetic acid, ethanol, and 2,3-butanediol obtained from the sugars labelled in carbon-1 contained C14 in the methyl groups. From the sugars labelled in carbon-2, A. aerogenes produced lactic acid, ethanol, and 2,3-butanediol labelled in the carbinol groups and acetic acid labelled in the carboxyl group. The results agree with the hypothesis that both sugars are fermented under anaerobic conditions by the Embden–Meyerhof–Parnas route. This route is also of major importance under aerobic conditions where little sugar appears to be dissimilated via the hexose monophosphate shunt.

PRODUCTION AND PROPERTIES OF 2,3-BUTANEDIOL: XVII. FERMENTATION OF GLUCOSE BY STRAINS OF BACILLUS SUBTILIS

1947 ◽  
Vol 25b (1) ◽  
pp. 56-64 ◽  
Author(s):  
A. C. Blackwood ◽  
A. C. Neish ◽  
W. E. Brown ◽  
G. A. Ledingham
Keyword(s):  
Carbon Dioxide ◽  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Anaerobic Conditions ◽  
Separate Species ◽  
Aerobic Conditions ◽  
Ferment Glucose

Under aerobic conditions both the Marburg and Ford types of Bacillus subtilis dissimilate glucose, giving carbon dioxide, acetoin, and 2,3-butanediol as the main products, and small amounts of glycerol and acetic, formic, lactic, and n-butyric acids. The Ford type cultures dissimilate the sugar more rapidly and also give a small amount of ethanol.Under anaerobic conditions Marburg type cultures will not ferment glucose while the Ford type cultures dissimilate it as rapidly as they do under aerobic conditions. Some strains give 2,3-butanediol, glycerol, and carbon dioxide as the major products while others give chiefly lactic acid. As much as 86% and as little as 8% of the glucose was converted to 2,3-butanediol plus glycerol. The 2,3-butanediol was a mixture of 65% meso- and 35% levo-isomers, while the lactic acid was approximately 90% dextro-isomer in most cases.It is suggested that the Ford type deserves separate species rank.

The Reaction of Thiol Compounds with the Vitamin-K Dependent Clotting Factors

1969 ◽  
Vol 21 (03) ◽  
pp. 573-579 ◽  
Author(s):  
P Fantl
Keyword(s):  
Prothrombin Time ◽  
Vitamin K ◽  
Anaerobic Conditions ◽  
Clotting Factors ◽  
Thiol Compounds ◽  
Aerobic Conditions ◽  
One Stage ◽  
Factor Ii ◽  
Ph Dependent ◽  
The One

SummaryTreatment of human and dog oxalated plasma with 0.2 to 1.0 × 10−1 M 2.3-dithiopropanol (BAL) or dithiothreitol (DTT) at 2–4° C for 30 min results in the reduction of the vitamin-K dependent clotting factors II, VII, IX and X to the respective-SH derivatives. The reaction is pH dependent. Under aerobic conditions the delayed one stage prothrombin time can be partly reversed. Under anaerobic conditions a gradual prolongation of the one stage prothrombin time occurs without reversal.In very diluted plasma treated with the dithiols, prothrombin can be converted into thrombin if serum as source of active factors VII and X is added. In contrast SH factors VII, IX and X are inactive in the specific tests. Reoxidation to active factors II, VII, IX and X takes place during adsorption and elution of the SH derivatives. The experiments have indicated that not only factor II but also factors VII, IX and X have active-S-S-centres.

Impact of storage duration and micro-aerobic conditions on lactic acid production from food waste

2021 ◽  
Vol 323 ◽  
pp. 124618
Author(s):  
Zengshuai Zhang ◽  
Panagiotis Tsapekos ◽  
Merlin Alvarado-Morales ◽  
Irini Angelidaki
Keyword(s):  
Lactic Acid ◽  
Food Waste ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Storage Duration ◽  
Aerobic Conditions

scholarly journals Recovery heat in muscular contraction without lactic acid formation

1931 ◽  
Vol 108 (757) ◽  
pp. 279-301 ◽  
Keyword(s):  
Acetic Acid ◽  
Oxygen Consumption ◽  
Lactic Acid ◽  
Muscular Contraction ◽  
Acid Formation ◽  
Anaerobic Conditions ◽  
Spontaneous Breakdown ◽  
Rate Of Oxygen Consumption ◽  
Resting Muscle

In a comparison of muscles poisoned with mono-iodo-acetic acid (IAA) in the presence and in the absence of oxygen respectively, Lundsgaard (1930) found:- (1) That the spontaneous breakdown of phosphagen in poisoned resting muscle is much more rapid under anaerobic conditions. (2) That the onset of the characteristic contracture produced by IAA is accompanied always by an increase in the rate of oxygen consumption.

Performance of aerobic granular sludge at variable circulation rate in anaerobic–aerobic conditions

2014 ◽  
Vol 69 (11) ◽  
pp. 2252-2257 ◽  
Author(s):  
Hasnida Harun ◽  
Aznah Nor Anuar ◽  
Zaini Ujang ◽  
Noor Hasyimah Rosman ◽  
Inawati Othman
Keyword(s):  
Municipal Wastewater ◽  
Granular Sludge ◽  
Ammonia Removal ◽  
Soy Sauce ◽  
Volume Index ◽  
Aerobic Granular Sludge ◽  
Circulation Rate ◽  
Anaerobic Conditions ◽  
Aerobic Conditions ◽  
Mixed Liquor

Aerobic granular sludge (AGS) has been applied to treat a broad range of industrial and municipal wastewater. AGS can be developed in a sequencing batch reactor (SBR) with alternating anaerobic–aerobic conditions. To provide anaerobic conditions, the mixed liquor is allowed to circulate in the reactor without air supply. The circulation flow rate of mixed liquor in anaerobic condition is the most important parameter of operation in the anaerobic-AGS processes. Therefore, this study investigates the effect of circulation rate on the performance of the SBR with AGS. Two identical reactors namely R1 and R2 were operated using fermented soy sauce wastewater at circulation rate of 14.4 and 36.0 l/h, respectively. During the anaerobic conditions, the wastewater was pumped out from the upper part of the reactor and circulated back into the bottom of the reactor for 230 min. A compact and dense AGS was observed in both reactors with a similar diameter of 2.0 mm in average, although different circulation rates were adopted. The best reactor performance was achieved in R2 with chemical oxygen demand removal rate of 89%, 90% total phosphorus removal, 79% ammonia removal, 10.1 g/l of mixed liquor suspended solids and a sludge volume index of 25 ml/g.

Effect of Ensilage of Rye Treated with Formic Acid and Lactic Acid Bacteria Inoculant on Ruminal Fermentation Characteristics

2020 ◽  
Vol 40 (4) ◽  
pp. 244-250
Author(s):  
Jayeon Kim ◽  
◽  
Rajaraman Bharanidharan ◽  
Geumhwi Bang ◽  
Soonwoo Jeong ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Formic Acid ◽  
Ruminal Fermentation
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