VEGETATIVE PROPAGATION OF CONIFERS: XIII. ROOTING OF NORWAY SPRUCE CUTTINGS IN THE GREENHOUSE

1945 ◽  
Vol 23c (5) ◽  
pp. 150-165
Author(s):  
N. H. Grace ◽  
J. L. Farrar

Greenhouse propagation of Norway spruce cuttings in various media containing sand and peats resulted in 80% rooting in a peat-humus–sand medium. Canadian peat moss and Swedish peat media were inferior but the weekly addition of potassium acid phosphate and magnesium sulphate solutions to a Swedish peat medium resulted in the rooting of 96% of the cuttings. Beneficial effects of peat humus were related to available nutrient materials. Peat humus extracted with methyl alcohol and acetic acid did not affect rooting, but sodium hydroxide extraction, hot water extraction, and air drying at 110 °C. were harmful. Propagation under suboptimum conditions disclosed markedly beneficial effects from mixtures and mulches of peats, soil, and active silica, the active silica being particularly favourable in the mulched condition.Medium cuttings, 3 to 6 in. long, rooted 96%, and long cuttings, 6 to 10 in., 71%. Uncovered cuttings and those covered with celloglass were superior in their responses to those under either cheesecloth or factory cotton screens.Plant hormone chemicals, when applied in a talc carrier dust, tended to reduce rooting of Norway spruce cuttings; the injurious effects increased progressively with concentration over the range from 500 to 4000 p.p.m. Indolylbutyric acid showed neither damaging nor beneficial effects. Naphthylbutyric acid and potassium naphthylhexoate, while similar in effect, tended to be less injurious than naphthylacetic acid when used over a range of equivalent molecular concentrations. Application of naphthylbutyric acid at the 2000 p.p.m. level in a charcoal carrier resulted in rooting of 98% of the cuttings. Mean root length was increased by the application of 50 p.p.m. each of thiamin and nicotinic acid. While nutrient salts and naphthylbutyric acid, each separately, tended to reduce mean root length, the combination had no injurious effect.

Holzforschung ◽  
2011 ◽  
Vol 65 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Tao Song ◽  
Andrey Pranovich ◽  
Bjarne Holmbom

Abstract Ground spruce sapwood was extracted with pressurised hot water with addition of different amounts of sodium bicarbonate (NaHCO3) to find optimal pH conditions for extraction of hemicelluloses, especially of galactoglucomannans (GGMs). The extractions were carried out in an accelerated solvent extractor (ASE) at 170°C. When extraction was carried out with 2.5 mM NaHCO3 during 60–100 min extraction time (end-pH 3.9), more than 60% of the total extracted substances were hemicellulose-derived carbohydrates. Under these conditions, approximately 80% of the extracted carbohydrates were from GGMs (13% b.o. wood). At higher concentrations of NaHCO3, the yield of carbohydrates, especially those derived from GGMs, decreased. Hydrolytic depolymerisation of hemicelluloses occurring during extraction with plain water was largely inhibited in the presence of 2.5–5 mM NaHCO3. The hydrolytic deacetylation of GGM was diminished at low NaHCO3 concentrations but increased dramatically at higher NaHCO3 concentrations. It can be concluded that 2.5–5 mM NaHCO3 giving an end-pH value of 4 is better for GGM isolation than plain water.


1941 ◽  
Vol 19c (7) ◽  
pp. 257-266
Author(s):  
N. H. Grace ◽  
J. L. Farrar

Norway spruce cuttings were collected at intervals throughout the year, subjected to treatment with talc dusts containing from 5 up to 10,000 p.p.m. of indolylacetic acid, and propagated in outdoor frames. In some experiments, indolylacetic acid treatments were included in a series of dusts involving cane sugar, potassium acid phosphate, and organic mercurial disinfectants. Effects of wax coating of cuttings of Norway and white spruce and eastern white cedar were also considered.Indolylacetic acid treatment failed to have any general beneficial effects on rooting; concentrations of 8000 and 10,000 p.p.m. were usually injurious. However, treatment of apparently dormant cuttings, taken shortly before emergence of new growth, with 1000 p.p.m. gave 25% rooting, as compared to 8% for the controls, and tended to increase the length of root. Treatments with organic mercury, cane sugar, and potassium acid phosphate increased survival of new growth, and, in combination with indolylacetic acid, increased survival and root lengths.Talc treatment increased rooting to as much as 70% for cuttings planted in sand as compared to 25% for the controls, but had no effect on plantings in a sand–peat mixture. Talc effects were the most marked on cuttings taken before emergence of new growth and when planting was delayed for 24 hr. after treatment.Wax application had no effect on dormant spruce cuttings but was markedly injurious to summer collections. Injurious effects were reduced when wax was used in conjunction with indolylacetic acid treatment.


2010 ◽  
Vol 45 (2) ◽  
pp. 223-236 ◽  
Author(s):  
Kaisu Leppänen ◽  
Peter Spetz ◽  
Andrey Pranovich ◽  
Kari Hartonen ◽  
Veikko Kitunen ◽  
...  

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4443
Author(s):  
Jiangyan Huo ◽  
Min Lei ◽  
Feifei Li ◽  
Jinjun Hou ◽  
Zijia Zhang ◽  
...  

A novel homogeneous polysaccharide named GEP-1 was isolated and purified from Gastrodia elata (G. elata) by hot-water extraction, ethanol precipitation, and membrane separator. GEP-1, which has a molecular weight of 20.1 kDa, contains a polysaccharide framework comprised of only glucose. Methylation and NMR analysis showed that GEP-1 contained 1,3,6-linked-α-Glcp, 1,4-linked-α-Glcp, 1,4-linked-β-Glcp and 1,4,6-linked-α-Glcp. Interestingly, GEP-1 contained citric acid and repeating p-hydroxybenzyl alcohol as one branch. Furthermore, a bioactivity test showed that GEP-1 could significantly promote the growth of Akkermansia muciniphila (A. muciniphila) and Lacticaseibacillus paracasei (L.paracasei) strains. These results implied that GEP-1 might be useful for human by modulating gut microbiota.


2011 ◽  
Vol 291-294 ◽  
pp. 1339-1343
Author(s):  
Wen Bo Zhang ◽  
Hong Rui Li ◽  
Jun Tao ◽  
Bing Bing Dong

The research in this paper optimized the extraction technique of lentinan with ultrasonic assistant method on the basis of hot water extraction technique, and investigated the promoting function of ultrasound to polysaccharides extraction. Extraction condition was selected by means of orthogonal experimental design, four factors and three levels L9(34), after key elements were respectively chosen through single factor experiments. Comparison between optimal extraction parameters of two method, hot water extraction technique and ultrasonic assistant extraction technique, showed decreased extraction temperature and significantly shortened extraction time, which existed in the second means, improved the extraction efficiency. Lentinus edodes polysaccharide extracted with ultrasonic assistant technique, the extraction rate and polysaccharide content percentage increased 6.22% and 8.66% respectively, comparative to which extracted with hot water extraction technique.


Holzforschung ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nacera Benouadah ◽  
Andrey Pranovich ◽  
Djamel Aliouche ◽  
Jalel Labidi ◽  
Stefan Willför

AbstractThe effectiveness of pressurized hot-water extraction conditions for obtaining galactoglucomannans (GGMs) from Pinus halepensis suitable for applications like coatings and films packaging was investigated. For this purpose, high molar masses with high yields are required, presenting a serious challenge for hot-water extraction processes. The extraction of GGMs was carried out in an accelerated solvent extractor (ASE) and the isolation was performed by precipitation in ethanol. Three temperatures in the range 160–180 °C and five extraction times 5–90 min were tested in order to optimize extraction parameters of GGMs, avoiding thermal and chemical degradation in hot-water. Total dissolved solids (TDS) were determined gravimetrically after freeze-drying and weight average molar masses (Mw) were determined by high-performance size exclusion chromatography (HPSEC). Total non-cellulosic carbohydrates were determined by gas chromatography (GC) after acid methanolysis. Free monomers were additionally analyzed by GC. Lignin in water extracts was measured by an ultraviolet (UV) method. Acetic acid was determined after alkaline hydrolysis of acetyl groups and analyzed by HPSEC. The main parameters influencing the extraction processes of the GGMs, namely, extraction time and temperature were studied. Optimal extraction parameters of GGMs were identified at 170 °C and 20 min extraction time, with average Mw of extracted fraction of 7 kDa leading to a GGM yield of approximately 56 ${\text{mgg}}_{\text{o}.\text{d}.\text{m}}^{-1}$, corresponding to 6% on dry wood basis.


Fitoterapia ◽  
2018 ◽  
Vol 126 ◽  
pp. 65-68 ◽  
Author(s):  
Bianca J. Deans ◽  
Wesley J. Olivier ◽  
David Girbino ◽  
Alex C. Bissember ◽  
Jason A. Smith

Sign in / Sign up

Export Citation Format

Share Document