THE PROBLEM OF PLASTEIN FORMATION: III. A NOTE ON THE COMPLEXITY OF PEPTIC PLASTEIN IN UREA SOLUTION

1940 ◽  
Vol 18b (10) ◽  
pp. 305-308 ◽  
Author(s):  
H. B. Collier
Keyword(s):  
Anaphylactic Reaction ◽  
Guinea Pigs ◽  
Enzymatic Synthesis ◽  
Urea Solution ◽  
Protein Properties

A solution of plastein in strong urea was injected into guinea-pigs and was found to give a slight anaphylactic reaction. The urea solution was also analysed in the velocity ultracentrifuge, and was shown to contain particles of protein dimensions, but sedimented in a completely inhomogeneous manner. The proteose substrate showed no sedimentation.It is concluded that, although the plasteins are formed as a result of a true enzymatic synthesis, and have protein properties, they are not definite entities comparable to typical proteins.

The passive anaphylactic reaction in guinea pigs elicited by whole and cathepsin D-degraded antigen

1974 ◽  
Vol 19 (1) ◽  
pp. 71-78
Author(s):  
J. Štefanovič ◽  
D. Kotulová ◽  
L’. Bergendi ◽  
I. Huzuláková
Keyword(s):  
Anaphylactic Reaction ◽  
Guinea Pigs ◽  
Cathepsin D

scholarly journals ALLERGIC IRRITABILITY

1928 ◽  
Vol 47 (3) ◽  
pp. 437-448 ◽  
Author(s):  
Paul A. Lewis ◽  
Dorothy Loomis
Keyword(s):  
Anaphylactic Reaction ◽  
Antibody Production ◽  
Guinea Pigs ◽  
Natural Resistance ◽  
Experimental Result ◽  
Second Phase ◽  
Reaction Complex

The allergic irritability of closely inbred guinea pigs as represented by their capacity to produce hemolytic antibodies for beef and sheep corpuscles, and agglutinins for Bacillus typhosus and Bacillus abortus (Bang) differs by families and therefore is at least partly dependent on inherited characteristics. These differences show an imperfect but suggestive correlation with the differences in resistance of the same families to inoculation tuberculosis as previously determined by Wright and Lewis. The differences in antibody production also show an imperfect correlation with the differences in response in the anaphylactic reaction complex as previously determined by Lewis and Loomis. These studies suggest very strongly that the allergic irritability is one of the several inheritable characters which form a partial basis for the natural resistance to tuberculosis. The antibody-producing capacity is only satisfactorily defined when minimal or moderate amounts of antigen are used and this in single treatments. The irregularities in experimental result when repeated treatments or very large single treatments are used suggest that antibody production in the second or "acquired capacity" phase may rest on a somewhat different fundamental basis than the latent or potential natural capacity. There is some very slight evidence that production in the second phase may also be influenced by inherited qualities.

scholarly journals THE RELATION BETWEEN ANTIANAPHYLAXIS AND ANTIBODY BALANCE

1936 ◽  
Vol 64 (4) ◽  
pp. 641-655 ◽  
Author(s):  
Marion C. Morris
Keyword(s):  
Guinea Pig ◽  
Intravenous Injection ◽  
Anaphylactic Reaction ◽  
Guinea Pigs ◽  
Normal Serum ◽  
Intravenous Inoculation ◽  
Lethal Doses ◽  
Circulating Antibody ◽  
Pig Serum ◽  
Increased Susceptibility

1. Sensitized guinea pigs injected with normal rabbit or guinea pig serum previous to intravenous inoculation of antigen may be protected against a few lethal doses of antigen. The protection is greater with foreign than with homologous serum and appears to be related roughly to the amount of serum introduced. 2. Sensitized guinea pigs injected with antibody-containing serum preliminary to intravenous injection of antigen, show no greater refractoriness to anaphylaxis than do those injected with normal serum. 3. Moreover, in many instances, the injection of an excess of antibody into the circulation of sensitized guinea pigs, leads to an increased susceptibility of these animals to anaphylaxis. 4. These results indicate that an excess of circulating antibody is not responsible for a state of antianaphylaxis, but on the contrary, may contribute toward the anaphylactic reaction itself.

scholarly journals CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS

1929 ◽  
Vol 50 (5) ◽  
pp. 551-567 ◽  
Author(s):  
William S. Tillett ◽  
Oswald T. Avery ◽  
Walther F. Goebel
Keyword(s):  
Anaphylactic Reaction ◽  
Protein Complex ◽  
Guinea Pigs ◽  
Protein Fraction ◽  
Anaphylactic Shock ◽  
Protective Action ◽  
Species Specificity ◽  
Carbohydrate Component ◽  
Serum Globulin ◽  
The Common

1. Guinea pigs passively sensitized with the serum of rabbits immunized with an artificially prepared sugar-protein (gluco-globulin) exhibit typical anaphylactic shock when subsequently inoculated with gluco-albumin; the serum of rabbits immunized with a second synthetic sugar-protein (galacto-globulin) similarly sensitizes guinea pigs to galacto-albumin. The reactions, in each instance, are specific and depend for their specificity on the carbohydrate component, and not on the protein fraction of the synthesized sugar-protein. 2. Guinea pigs actively sensitized with gluco-globulin or galacto-globulin are similarly subject to anaphylactic shock, when injected, after 21 days, with sugar-proteins containing carbohydrate identical with that present in the sensitizing antigen, regardless of the kind of protein with which it is combined. 3. The unconjugated glucosides, although themselves not capable of inducing shock, inhibit the anaphylactic reaction when injected immediately prior to the introduction of the toxigenic sugar-protein. The protective action of the glucosides disappears within two hours after injection. In order to elicit the phenomenon, the carbohydrate must be the same as that combined in the sugar-protein complex. 4. Anaphylactic shock may be induced by uncombined globulin in guinea pigs passively sensitized with either antigluco-globulin serum or antigalacto-globulin serum; globulin is similarly effective in animals actively sensitized with gluco-globulin or galacto-globulin. The reactions elicited by globulin alone are dependent upon the common protein present in the antigens, and exhibit only species specificity.

Airway Epithelial Cells in Various Models of Bronchial Allergic Inflammation in Guinea Pigs

1992 ◽  
Vol 5 (3) ◽  
pp. 165-172
Author(s):  
J.P. Tarayre ◽  
M. Aliaga ◽  
M. Barbara ◽  
N. Malfetes ◽  
S. Vieu
Keyword(s):  
Epithelial Cells ◽  
Anaphylactic Reaction ◽  
Guinea Pigs ◽  
Allergic Inflammation ◽  
Lavage Fluid ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Similar Intensity

The number of airway epithelial cells (AEC) in bronchus lumen after various types of anaphylactic shock of similar intensity was determined in guinea pigs. Aerosol-induced anaphylactic shocks were studied after four types of active sensitization: sensitization by IM injection of 30 mg/kg ovalbumin mixed with Freund's complete adjuvant; sensitization by IM injection of 30 mg/kg ovalbumin; sensitization by IP injection of 1 μg ovalbumin and 50 mg A1 (OH)3 by animal; sensitization by 2 exposures to 1%-ovalbumin aerosol at a 7-day interval. One, 3,6,24 and 48 h after the anaphylactic reaction the number of AEC in bronchoalveolar lavage fluid (BALF) was counted in comparison to guinea pigs sensitized but exposed to 0.9% NaCl aerosol. The number of AEC was significantly increased only 24–48 h after the shock in animals sensitized with ovalbumin in Freund's complete adjuvant. In animals passively sensitized by antiserum obtained with sensitization by ovalbumin in Freund's complete adjuvant and challenged by IV or aerosol administration of antigen, non rise in the number of AEC was obtained 24–48 h after the shock. The higher amount of AEC 24–48 h after the anaphylactic reaction in animals actively sensitized by ovalbumin in Freund's complete adjuvant could be related to the higher increase in the number of neutrophils and of mononuclears obtained in the BALF at these times in comparison to other types of active sensitization. The rise in the number of AEC only after the active reaction seems to show the part played by T-lymphocytes in the induction of this effect. The increase in the number of eosinophils in BALF and the appearance of the hyperreactivity cannot be related to the rise in the AEC in bronchus lumen.

Benzylpenicillin preparations can evoke a systemic anaphylactic reaction in guinea pigs

1978 ◽  
Vol 62 (5) ◽  
pp. 276-282 ◽  
Author(s):  
Masaharu Muranaka ◽  
Shuji Suzuki ◽  
Kazuhiro Koizumi ◽  
Hiroshi Igarashi ◽  
Hiroshi Okumura ◽  
...  
Keyword(s):  
Anaphylactic Reaction ◽  
Guinea Pigs
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