FROST-HARDENING STUDIES WITH LIVING CELLS: I. OSMOTIC AND BOUND WATER CHANGES IN RELATION TO FROST RESISTANCE AND THE SEASONAL CYCLE

1936 ◽  
Vol 14c (7) ◽  
pp. 267-284 ◽  
Author(s):  
J. Levitt ◽  
G. W. Scarth
Keyword(s):  
Osmotic Pressure ◽  
Cell Volume ◽  
Low Temperatures ◽  
Frost Resistance ◽  
Woody Plants ◽  
Seasonal Cycle ◽  
Bound Water ◽  
Living Cells ◽  
Ice Formation ◽  
Frost Hardening

The osmotic pressure and non-solvent space of the cells of various types of plant were estimated by the plasmolytic method and related to frost resistance and the seasonal cycle.Osmotic pressure always rises with hardening and falls with dehardening, and it generally reaches higher values or begins to rise earlier in the hardier species and varieties.The effect of osmotic pressure in reducing the amount of ice formation is enhanced in woody plants by the condition that only about half the cell volume is occupied by the osmotically active solution. The remainder, i.e., the non-solvent space, is shown to consist partly of bound water and must therefore represent hydrophilic colloid. This occupies an even larger proportion of the sap vacuole than of the protoplasm, and it increases notably with hardening. This change, besides reducing intercellular ice, is regarded as protecting the most vulnerable part of the cell, viz., the vacuole, from being frozen at very low temperatures.

FROST-HARDENING STUDIES WITH LIVING CELLS: II. PERMEABILITY IN RELATION TO FROST RESISTANCE AND THE SEASONAL CYCLE

1936 ◽  
Vol 14c (8) ◽  
pp. 285-305 ◽  
Author(s):  
J. Levitt ◽  
G. W. Scarth
Keyword(s):  
Small Molecules ◽  
Seasonal Changes ◽  
Frost Resistance ◽  
Woody Plants ◽  
Seasonal Cycle ◽  
Potassium Nitrate ◽  
Living Cells ◽  
Cell Permeability ◽  
Hardened State ◽  
Permeability Increase

Cell permeability as estimated by the plasmolytic method in various types of plants and for different classes of solute has been studied in relation to frost resistance and the seasonal cycle. Permeability is found to increase greatly with hardening, whether induced by low temperature or other conditions, and it seems to parallel closely the seasonal changes in frost resistance. As regards different species and varieties, cell permeability in the hardened state shows better correlation than any other character with ability to resist frost. The permeability change is greatest towards potassium nitrate—at least in cells (viz., those of hardy woody plants) that are definitely permeable to the salt; the change is more moderate towards polar non-electrolytes with small molecules, such as urea, but with these it occurs in all plants capable of hardening; towards apolar substances, such as urethane, there is no change. These relations point to a widening of the aqueous pores or increased hydration of the plasma membrane as the mechanism of the permeability increase. Hypotheses are put forward as to the means by which freer permeability to water may increase resistance to certain types of mechanical injury by frost.

scholarly journals THE OSMOTIC PROPERTIES OF LIVING CELLS (EGGS OF ARBACIA PUNCTULATA)

1931 ◽  
Vol 14 (3) ◽  
pp. 393-403 ◽  
Author(s):  
Morton McCutcheon ◽  
Balduin Lucké ◽  
H. Keffer Hartline
Keyword(s):  
Surface Tension ◽  
Osmotic Pressure ◽  
Cell Volume ◽  
Sea Water ◽  
Living Cells ◽  
Arbacia Punctulata ◽  
Wide Range ◽  
Inactive Cell ◽  
Osmotic Properties

We have attempted to answer the question: How nearly ideal, as an osmometer, is the unfertilized Arbacia egg? The following conclusion have been reached: 1. Volumes can be measured accurately over a wide range of pressures since the cell is in general spherical and does not suffer deformation from its own weight or other factors. 2. The product of volume and pressure is approximately constant, if allowance be made for osmotically inactive cell contents. It is computed that from 7 to 14 per cent of cell volume is occupied by osmotically inactive material. 3. Evidence is presented that no appreciable escape of cell contents occurs while the cell is in hypotonic sea water; that, therefore, the semipermeability of the membrane is approximately perfect, so long as injury to the cell is avoided. 4. In comparison with osmotic pressure the influence of other forces, such as elasticity or surface tension, on cell volume must in these experiments be slight.

Features of radiothermoluminescence of polypropylene and ethylene propylenediene elastomer SKEPT-4044 compositions with nanoscale metal-containing fillers

2020 ◽  
pp. 66-73
Author(s):  
A.M. Magerramov ◽  
◽  
N.I. Kurbanova ◽  
M.N. Bayramov ◽  
N.A. Alimirzoyeva ◽  
...  
Keyword(s):  
Transition Temperature ◽  
Molecular Mobility ◽  
Fe3o4 Nanoparticles ◽  
Low Temperatures ◽  
Frost Resistance ◽  
Relaxation Processes ◽  
Ethylene Propylene ◽  
Temperature Range ◽  
Β Relaxation

Using radiothermoluminescence (RTL), the molecular mobility features in the temperature range of 77-300 K were studied for the polypropylene (PP)/ethylene propylene diene elastomer SKEPT-4044 with NiO, Cu2O and Fe3O4 nanoparticles (NPs) based on ABS-acrylonitrile butadiene or SCS-divinyl styrene matrices. It has been shown that the introduction of nanofillers in PP significantly affects the nature and temperature of γ- and β-relaxation processes, while the region of manifestation of the β-process noticeably shifts to the region of low temperatures. Composites with Cu2O NPs have a higher β-transition temperature Tβ than composites with other NPs. It was found that PP/SKEPT-4044 composites with Cu2O NPs with a dispersion of 11-15 nm and acrylonitrile butadiene thermoplastics have optimal frost resistance compared to other compositions.

Colloid osmotic pressure changes of dog's blood exposed to different mixtures of CO2 and air

1978 ◽  
Vol 44 (2) ◽  
pp. 254-257 ◽  
Author(s):  
Y. Kakiuchi ◽  
A. B. DuBois ◽  
D. Gorenberg
Keyword(s):  
Red Blood Cells ◽  
Osmotic Pressure ◽  
Cell Volume ◽  
Blood Cells ◽  
Freezing Point ◽  
Colloid Osmotic Pressure ◽  
Red Cell ◽  
Freezing Point Depression ◽  
Pressure Changes ◽  
Different Levels

Hansen's membrane manometer method for measuring plasma colloid osmotic pressure was used to obtain the osmolality changes of dogs breathing different levels of CO2. Osmotic pressure was converted to osmolality by calibration of the manometer with saline and plasma, using freezing point depression osmometry. The addition of 10 vol% of CO2 to tonometered blood caused about a 2.0 mosmol/kg H2O increase of osmolality, or 1.2% increase of red blood cell volume. The swelling of the red blood cells was probably due to osmosis caused by Cl- exchanged for the HCO3- which was produced rapidly by carbonic anhydrase present in the red blood cells. The change in colloid osmotic pressure accompanying a change in co2 tension was measured on blood obtained from dogs breathing different CO2 mixtures. It was approximately 0.14 mosmol/kg H2O per Torr Pco2. The corresponding change in red cell volume could not be calculated from this because water can exchange between the plasma and tissues.

scholarly journals THE KINETICS OF EXOSMOSIS OF WATER FROM LIVING CELLS

1927 ◽  
Vol 10 (5) ◽  
pp. 659-664 ◽  
Author(s):  
Morton McCutcheon ◽  
Baldwin Lucke
Keyword(s):  
Osmotic Pressure ◽  
Salt Concentration ◽  
Driving Force ◽  
Temperature Characteristic ◽  
Living Cells ◽  
Velocity Constant ◽  
Osmotic Equilibrium ◽  
Kinetics Of

1. The rate of exosmosis of water was studied in unfertilized Arbacia eggs, in order to bring out possible differences between the kinetics of exosmosis and endosmosis. 2. Exosmosis, like endosmosis, is found to follow the equation See PDF for Equation, in which a is the total volume of water that will leave the cell before osmotic equilibrium is attained, x is the volume that has already left the cell at time t, and k is the velocity constant. 3. The velocity constants of the two processes are equal, provided the salt concentration of the medium is the same. 4. The temperature characteristic of exosmosis, as of endomosis, is high. 5. It is concluded that the kinetics of exosmosis and endosmosis of water in these cells are identical, the only difference in the processes being in the direction of the driving force of osmotic pressure.

Influence of Lightweight Aggregate on Durability of High Performance Concrete

2009 ◽  
Vol 405-406 ◽  
pp. 197-203
Author(s):  
Bao Sheng Zhang ◽  
Li Juan Kong ◽  
Yong Ge
Keyword(s):  
Pore Structure ◽  
Cement Paste ◽  
Frost Resistance ◽  
High Performance ◽  
High Performance Concrete ◽  
Bound Water ◽  
Lightweight Aggregate ◽  
Normal Weight ◽  
Lightweight Aggregate Concrete ◽  
Aggregate Concrete

High performance concrete (HPC) with a water/cement ratio (w/c) of 0.32 and different lightweight aggregate (LWA) contents (0%, 25%, 50%, 75%, 100%) were prepared, and the influence of LWA on concrete frost-resistance and impermeability at different ages were studied, as well as the hydration degree, hydrated product, pattern and pore structure of the paste around aggregate. The results show that, by replacing normal weight aggregate (NWA) with 50% and 100% volume contents of pre-wetted LWA respectively, the chemical bound water of the cement paste surrounding aggregate are increased 12.1% and 22.7% as compared to concrete mixed without LWA. And at 28 days, lightweight aggregate concrete has the highest Ca(OH)2 content, whereas the 90-day Ca(OH)2 content of normal weight concrete is the highest. This proves that, with the increase of LWA content in concrete, both of the internal curing effect of pre-wetted LWA and secondary hydration effect of fly ash (FA) are strengthened, this can also be verified by the SEM study. Furthermore, the pore structure of the cement paste around aggregate can be improved consequently. The performance of frost-resistance of HPC can be improved by mixing LWA, the 90 day-frost-resistance of lightweight aggregate concrete is about 2.5 times of that of concrete mixed without LWA. The influence of LWA on the impermeability of HPC is different from normal concrete. When LWA content is more than 50%, the HPC impermeability decreased obviously, however at later age the difference between them becomes minor.

Effect of basement membrane and colloid osmotic pressure on renal tubule cell volume

1977 ◽  
Vol 233 (4) ◽  
pp. F325-F332
Author(s):  
M. A. Linshaw ◽  
F. B. Stapleton ◽  
F. E. Cuppage ◽  
J. J. Grantham
Keyword(s):  
Basement Membrane ◽  
Osmotic Pressure ◽  
Cell Volume ◽  
Cell Size ◽  
Renal Tubule ◽  
Colloid Osmotic Pressure ◽  
Outer Diameter ◽  
Tubule Cell ◽  
Cation Pump ◽  
Renal Tubule Cell

Renal tubule cell volume is thought to be kept constant by a cation pump. When active transport is blocked, intracellular impermeant solutes cause cells to swell. Cell size is then determined by transmembrane hydrostatic and colloid osmotic forces. We studied the importance of passive transmembrane forces in determining cell size in isolated rabbit proximal straight tubules (PST). We blocked active solute transport with ouabain and evaluated subsequent changes in cell size by measuring outer diameter of nonperfused tubules. Tubules in a ouabain and 6 g/100 ml protein bath swelled only 40% above control. However, removal of the tubule basement membrane with collagenase dissipated a transmembrane hydrostatic pressure and caused more swelling. Final cell volume was determined largely by bath protein concentration. Tubules in ouabain and collagenase swelled enormously in hyponcotic protein, moderately in isoncotic protein, and could be shrunk below control in hyperoncotic protein. Intracellular colloid osmotic pressure was estimated to exceed 38 cmH20. We conclude that hydrostatic and colloid osmotic forces are major determinants of cell size in isolated PST treated with ouabain.

scholarly journals Modyfikowanie HTPB (α,ω-dihydroksypolibutadienu) w reakcjach estryfikacji, silanizacji, epoksydacji i uwodornienia

2018 ◽  
pp. 30-45
Author(s):  
Michał Chmielarek ◽  
Paweł Maksimowski ◽  
Tomasz Gołofit ◽  
Katarzyna Cieślak ◽  
Wojciech Pawłowski ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Low Temperatures ◽  
Frost Resistance ◽  
High Energy ◽  
Insulating Materials ◽  
Rocket Propulsion ◽  
Materials Research ◽  
Selection Of

HTPB is a valuable comonomer for the preparation of polyurethanes. Thanks to its specific properties, it gives these materials frost resistance and excellent mechanical properties, especially at low temperatures. The polyurethanes thus obtained are used in the production of propellants used in space and military rocket propulsion, and are the basis for frost-resistant adhesives and insulating materials. Research on the selection of binder indicates the use of high energy polymers or modifications of previously used polymers improving their properties. The results of research on ways of changing the properties of HTPB through its modification and thus an increase of application possibilities will be presented.

scholarly journals Characterization of Suspension Cultures of Arabidopsis thaliana (L.) Heynh Cells with Altered Expression of the Gene of Alternative Mitochondrial Oxidase Aox1a and Analysis of their Frost Resistance

2021 ◽  
Vol 35 ◽  
pp. 3-18
Author(s):  
A. V. Stepanov ◽  
◽  
S. A. Kashin ◽  
N. S. Zabanova ◽  
O. A. Fedotova ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Suspension Culture ◽  
Mitochondrial Respiration ◽  
Frost Resistance ◽  
Alternative Oxidase ◽  
Uncoupling Protein ◽  
Living Cells ◽  
Subzero Temperature ◽  
Wild Type ◽  
Culture Cells

The enzyme alternative cyanide-resistant oxidase (AOX) localized in mitochondria is involved in the processes of plant adaptation to various unfavorable biotic and abiotic factors. Transfer of electrons from ubiquinone to oxygen by alternative oxidase has a nonprotonmotive character and, by bypassing two sites of H+ pumping in complexes III and IV, lowers the energy efficiency of respiration and energy of electron flow through AOX is released as heat. In this work, we characterized heterotrophic suspension cultures of Arabidopsis thaliana (L.) Heynh cells obtained from seeds of plants with altered (reduced (AS-12 line) and increased (XX-2 line)) expression of the alternative oxidase gene AOX1a and studied their viability under subzero temperature (-10 °С for 3, 6, 9 hours). Cell viability and reactive oxygen species (ROS) production were assessed using fluorescence microscopy with fluorescein diacetate (FDA) and propidium iodide (PI) for cell viability measurement and H2DCF-DA for ROS measurement. The proportion of living cells was calculated as the proportion of FDApositive and PI-negative cells. Differences between the studied lines were determined in the content of mitochondrial proteins of the respiratory chain (AOX, COXII, NDB) and uncoupling protein (UCP), as well as in the intensity of formation of ROS and frost resistance. The obtained results confirmed the higher content of the AOX protein and its high contribution to mitochondrial respiration in line XX-2. Suspension culture cells of the AS-12 line showed a decrease in the AOX protein content and its contribution to mitochondrial respiration, compared to the wild type (Col-0) and line XX-2. Simultaneously with a decrease in the AOX protein content in the AS-12 cell culture, an increase in the content of the uncoupling protein UCP and subunit II of cytochrome oxidase (COXII) was observed. ROS generation was reduced in cell cultures of both XX-2 and AS-12. The obtained results indicate that the cells of the wildtype (Col-0) suspension culture were subjected to the most significant effect of subzero temperature. Long-term exposure (for 9 h) under -10 °С revealed significant differences in the viability of wild-type culture cells and lines with altered AOX1a gene expression. Cells of line XX-2 with an increased content of AOX turned out to be more resistant to subzero temperature compared to wild-type and AS-12 cells. However, while the proportion of living cells in the culture of the AS-12 line 48 h after exposure remained at the same level as immediately after it, in the suspension culture of the wild type cell death developed over time. The obtained results indicate the importance of alternative oxidase in the development of frost resistance in plant cell.

Ice Formation as an Indicator of Frost-Resistance on the Concrete Containing Slag Cement in Conditions of Freezing and Thawing

2016 ◽  
Vol 865 ◽  
pp. 145-150
Author(s):  
Oleksandr Moskalenko ◽  
Rayisa Runova
Keyword(s):  
Frost Resistance ◽  
Ice Formation ◽  
Cement Concrete ◽  
Chemical Additives ◽  
Freezing And Thawing ◽  
Slag Cement ◽  
Mineral Supplement

The connection with the icing on the frost resistance of concrete containing slag cement and chemical additives «MC Bauchemie» under freezing and thawing.It is shown that freeze concrete samples at (-) 10°C increase in the amount of slag from 30 to 70 wt.% Of binder in the composition leads to an increase in the index of ice formation in the concrete of 1.7 ... 1.9 times compared with the concrete obtained at a slag containing slag cement with a content of 10 wt.%. Frost resistance of concrete is reduce from F450 to F400.When freezing of concrete samples at (-) 20°C increase in the amount of slag from 30 to 50 wt. % Binder in the composition leads to an increase in the index of ice formation in the concrete of 1.7 times compared with the concrete obtained on slag cement containing slag with a content of 10 wt.%. Frost resistance of concrete is reduce from F400 to F350.The concrete on the slag containing cement with slag 70 wt. % is observed a slight decrease in ice formation. However, its value is 1.4 times higher than ice formation in concretes containing slag in an amount of 10 wt. %. Mark on frost resistance remains at F350.The smallest ice formation, regardless of the content of the slag into the slag containing cement, concrete characterized in that use complex organo-mineral supplement SX (5%) + SP (0.6%) in the amount of 5.6%. According to the degree of influence of additives used to reduce ice formation in the slag in concretes, containing cements can be ranker number: SX (5%) + SP (0.6%) > NC (5%) + SP (0.6%) > SP (0.6%).

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