MICROBIOLOGICAL STUDIES OF APPALACHIAN PODSOL SOILS: II. SEASONAL CHANGES IN MICROBIAL ACTIVITY
Seasonal fluctuations in numbers of micro-organisms in soil, estimated by the plate method for bacteria and actinomyces and by the evolution of carbon dioxide, have been studied with samples of cultivated podsol soils, drawn from experimental fields under various treatments at three farms in the Eastern Townships region of Quebec, at intervals of six weeks during the cropping season in two years. The nature of the fluctuations in numbers of micro-organisms was similar in the three soils, which were situated many miles apart. In 1931 numbers were lower in summer than in spring and autumn; in 1932 numbers fell towards the end of the season. The evolution of carbon dioxide fluctuated to a comparatively less extent than the bacterial numbers, but there was general agreement in the direction of the changes in the two years. Biological activities in any one soil were at significantly different levels in the two years. There was an agreement between numbers of micro-organisms and the amount of carbon dioxide, in that changes in both of these factors were in the same direction in the second year of sampling; there was also evidence of agreement between intra-annual changes in numbers and carbon dioxide, especially in the alkali-treated plots. Fluctuations in numbers and carbon dioxide in samples from plots in different parts of the same field or farm were in conformity, and were superimposed on the effects of chemical treatments that caused significant increases in microbial activity.