THE ALKALOIDS OF FUMARIACEOUS PLANTS: VIII. CORYDALIS AUREA, WILLD. AND THE CONSTITUTION OF BICUCINE

1933 ◽  
Vol 9 (5) ◽  
pp. 436-442 ◽  
Author(s):  
R. H. F. Manske
Keyword(s):  
Empirical Formula ◽  
Hydroxy Acid ◽  
Phenolic Hydroxyl ◽  
High Concentration ◽  
Chemical Examination ◽  
Low Concentration ◽  
Isolation And Characterization ◽  
Stems And Leaves ◽  
Constitutional Analysis

The chemical examination of the alkaloids of Corydalis aurea has shown an unusual complexity and of the total of more than ten alkaloids thus far isolated only six are now described. The record deals chiefly with the stems and leaves of the plant in which protopine was present in exceptionally low concentration (0.025%). Equally exceptional is its high concentration m the roots (1.6%). l-Tetrahydropalmatine constituted the largest fraction of the remaining alkaloids, and its present isolation is the first on record although the d-form was previously known. Two new and well-characterized alkaloids, which have been named capaurine and capauridine, respectively, are isomeric and are best represented by the empirical formula C21H27O5N. Both contain one phenolic hydroxyl and four methoxyl groups, and yield on methylation non-phenolic bases which do not appear to be identical. The presence of two bases, bicuculline and bicucine, which were first recorded in this series of papers, has again been demonstrated.The constitutional analysis of the new bases, as well as the isolation and characterization of the minor alkaloids, is in progress.In an appended note it is shown that bicuculline and bicucine are closely related and interconvertible. The latter is the free γ-hydroxy acid of which the former is the lactone.

scholarly journals Isolation and Characterization of Staphylococcus Aureus Phage and Its Anti-Biofilm Activity Individually or Collaborative with Streptomycin

2020 ◽  
Author(s):  
Liming Jiang ◽  
Rui Zheng
Keyword(s):  
Staphylococcus Aureus ◽  
Optimal Growth ◽  
High Concentration ◽  
Optimal Growth Temperature ◽  
Low Concentration ◽  
Isolation And Characterization ◽  
Slaughter House ◽  
Biological Surfaces ◽  
Wide Range

Abstract Background: Staphylococcus aureus was a widespread of Gram-positive pathogen bacteria which causes a wide range of symptoms. Bacteria biofilm was the multicellular community of microorganisms that attached to non-biological and biological surfaces. Method: Here, we aimed to isolation and characterization of S. aureus phage and research its bactericidal activity that individually or collaborative with streptomycin.Results: In this study, virulent phage WX was isolated from slaughter house in Jiangsu, China. It’s belonged to the Siphoviridae family and optimal growth temperature was 37 ℃, the pH of optimal preservation buffer was 6~7, optimal multiplicity of infection (MOI) was 0.01 and the genome size was 141, 342 bp. Phage WX has can sterilize most clinical strains of S. aureus which was isolated from clinical patients in the first people's hospital of Yunnan province laboratory. Streptomycin has better anti-biofilm effect than phage WX in low concentration culture of bacteria, nonetheless, phage WX has better anti-biofilm effect than streptomycin in high concentration culture of bacteria. Collaboration of phage WX and streptomycin have better anti-biofilm effect than alone of WX or streptomycin in low concentration culture of bacteria and phage WX have better anti-biofilm effect than streptomycin in high concentration culture of bacteria. Conclusion: The data of this study provided a strong evidence of application phage for reduce the growth of S. aureus biofilm, this study was important for clinic and replace antibiotics in some extent.

Isolation and characterization of lignocellulosic fiber from jute bast by the organic solvent degumming system: an alkali-free method

2021 ◽  
pp. 004051752110154
Author(s):  
Zhihui Qin ◽  
Shuyuan Zhao ◽  
Liu Liu ◽  
Zhaohe Shi ◽  
Longdi Cheng ◽  
...  
Keyword(s):  
National Standards ◽  
X Ray Diffraction ◽  
High Concentration ◽  
X Ray ◽  
Lignocellulosic Fibers ◽  
Lignocellulosic Fiber ◽  
Isolation And Characterization ◽  
Alkali Consumption ◽  
Scanning Electron

Degumming is the dominant method for insolating lignocellulosic fibers in textile applications. Traditional alkaline degumming (TAL), as a common method, requires a high-concentration alkali and has been a severe challenge to the environment. In the research reported here, the possibility of innovative jute degumming by organic solvents 1-2 propylene glycol and a combination of additive green oxygen (GO-OS) was studied. The results revealed that fibers could be extracted by this system (under condition of 0.9% GO-OS, 180°C, 120 min), and obtained fibers with higher breaking tenacity (7.1 cN/dtex), yield (65.7%), breaking elongation (2.87%) and residual gum (11.7%), which all meet the requirement of the relevant Chinese Textile National Standards. Notably, the required reaction time (120 min) of the GO-OS system was 180 min shorter than that of the TAL method. Furthermore, the modifications introduced by the degumming effect on physicochemical aspects were characterized and confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. This study provides a promising degumming method for separating jute lignocellulose without acid and alkali consumption.

Isolation and Characterization of Bifidobacteria from diverse ecosystems

2019 ◽  
Vol 8 (2) ◽  
pp. 43-48
Author(s):  
Halima Aurass Bahloul ◽  
Hadadji Miloud ◽  
Mebrouk Kihal
Keyword(s):  
Newborn Infants ◽  
Food Samples ◽  
High Concentration ◽  
Dominant Group ◽  
Human Gut ◽  
Important Place ◽  
Isolation And Characterization ◽  
Probiotic Strains ◽  
Commercial Milk

Bifidobacteria represent the largest group of human intes nal bacteria. They have an important place in human health and represent the dominant group of micro ora. For this reason, they are key members of the human gut microbiota, particularly during the first one to two years following birth. The aim of this study was to test the sensibility of Bifidobacterium isolated from some strains at different concentrations of salinity and high concentration of bile as a criterion to select probiotic strains. Following the behavior of strains of Bifidobacteria isolated from different ecosystems was considered in order to develop the therapeu c aliment food . Samples from newborn infants aged 3 months, bee intestine, fermented commercial milk and yoghurt of bifidus containing Bifidobacteria was used and the isolated strains belonged to -breve and -longum species. The results of resisitance of our species of Bifidobacteria demonstrated a total inibition of croissance at 5.5% of NaCl but in 5% media of NaCl we observed a moderate croissance at di erent me of kinetics croissance .

Isolation and characterization of Rhodococcus sp. NB5 capable of degrading a high concentration of nitrobenzene

2011 ◽  
Vol 51 (4) ◽  
pp. 397-403 ◽  
Author(s):  
Hui Lin ◽  
Xue-jiao Chen ◽  
Hai-tao Ding ◽  
Xiao-ming Jia ◽  
Yu-hua Zhao
Keyword(s):  
High Concentration ◽  
Isolation And Characterization ◽  
Rhodococcus Sp

scholarly journals Isolation and characterization of Escherichia coli phage and enhance its bactericidal activity that collaborative with kanamycin sulfate

2020 ◽  
Author(s):  
Liming Jiang ◽  
Rui Zheng
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Bactericidal Activity ◽  
Therapeutic Potential ◽  
Health Concern ◽  
Low Concentration ◽  
E Coli ◽  
Kanamycin Sulfate ◽  
Isolation And Characterization

Abstract Background: Escherichia coli is the most important and widespread bacteria in worldwide, which mainly found in contaminated food, human and animal faeces. Unfortunately, Some of E. coli strains are multidrug-resistant (MDR) pathogen leading significant public health concern globally. Biofilm is a multicellular community of microorganisms. Phages and their derivatives are ideal candidates for replacing or compensating for antibiotic problems in the future. Method: Here, we aimed to isolation and characterization of Escherichia coli phage and research its bactericidal activity that individually or collaborative with kanamycin sulfateResults: In this study, three virulent phages Flora, T4 and WJ were isolated from the laboratory and drug sample in Wuxi, China. It’s belonged to the Myoviridae family and optimum temperature is 42 ℃, optimum pH= 7, optimum MOI is 0.0001 and the genome size of Flora, T4 and WJ were 168, 909, 168903 and 168, 900 bp respectively. Flora has two exonuclease, whereas T4 and WJ have only one. Antibiotics have better bactericidal activity than phages in a low concentration medium of bacteria, nonetheless, phages have better bactericidal activity than antibiotics in a high concentration of bacteria, and that, collaboration of phages and antibiotics have better bactericidal activity effect than alone of phages or antibiotics in a low concentration medium of bacteria. Conclusion: The excellent performance of phage Flora for its therapeutic potential on clinic. The data of this study provided the strong evidence that the application of phage could reduce the growth and biofilm of E. coli that are important to maintain public health. Keywords: Escherichia coli, phage, lytic spectrum, biofilm, antibiotic

scholarly journals Isolation and characterization of a novelArabidopsis thatianamutant that requires a high concentration of boron

2004 ◽  
Vol 50 (7) ◽  
pp. 1183-1185
Author(s):  
Noriaki Aoki ◽  
Kyotaro Noguchi ◽  
Hiroaki Hayashi ◽  
Toru Fujiwara
Keyword(s):  
High Concentration ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of thenikR Gene Encoding a Nickel-Responsive Regulator inEscherichia coli

1999 ◽  
Vol 181 (2) ◽  
pp. 670-674 ◽  
Author(s):  
Karinne De Pina ◽  
Valerie Desjardin ◽  
Marie-Andree Mandrand-Berthelot ◽  
Gerard Giordano ◽  
Long-Fei Wu
Keyword(s):  
Promoter Region ◽  
Constitutive Expression ◽  
Inescherichia Coli ◽  
Start Site ◽  
High Concentration ◽  
Transcription Start ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Specific Transport System

ABSTRACT Expression of the nickel-specific transport system encoded by theEscherichia coli nikABCDE operon is repressed by a high concentration of nickel. By using random transposon Tn10 insertion, we isolated mutants in which expression of the nik operon became constitutive with respect to nickel. We have identified the corresponding nikR gene which encodes a nickel-responsive regulator. Expression of nikRwas partially controlled by Fnr through transcription from thenikA promoter region. In addition, a specific transcription start site for the constitutive expression of nikR was found 51 bp upstream of the nikR gene.

The isolation of a combined zirconium-chromium(III) basic sulphate complex

1971 ◽  
Vol 24 (4) ◽  
pp. 687 ◽  
Author(s):  
KC Montgomery ◽  
JG Scroggie
Keyword(s):  
Transition Metal ◽  
Transition Metal Complexes ◽  
Empirical Formula ◽  
Model Compound ◽  
Complex Anion ◽  
Disodium Salt ◽  
Leather Industry ◽  
Isolation And Characterization ◽  
Proteins And Peptides

The isolation and characterization of a relatively stable, highly basic zirconium-chromium(III) sulphate complex is described. This compound has an empirical formula corresponding to Na2O,3ZrO2,Cr2O3,4SO3,12H2O and appears to exist as the disodium salt of a complex anion Na2[Zr3Cr2(SO4)4(OH)12,6H2O]. Its properties indicate useful applications not only as a model compound to study the binding of transition metal complexes to proteins and peptides but also as a potential tanning agent for use in the leather industry.

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