Isolation and evaluation of three novel native promoters in Brassica napus

Botany ◽  
2013 ◽  
Vol 91 (6) ◽  
pp. 414-419 ◽  
Author(s):  
Limin Wu ◽  
Aliaa El-Mezawy ◽  
Saleh Shah
Keyword(s):  
Brassica Napus ◽  
Reporter Gene ◽  
Genetic Modification ◽  
Gus Activity ◽  
The Other ◽  
Genome Walking ◽  
Constitutive Promoter ◽  
Brassica Napus L ◽  
Transgenic Canola ◽  
Uida Reporter Gene

To provide effective and specific native promoters for canola (Brassica napus L.) genetic modification, three promoters were isolated by genome walking from this species. These three promoters were fused to the uidA reporter gene (GUS) and were independently used to generate populations of transgenic canola plants. Plants transformed with BnPGPro-GUS (B. napus putative germin promoter) exhibited GUS activity in all the tissues tested at a level comparable to those transformed with CaMV35 S promoter. This indicates that BnPGPro may serve as a native constitutive promoter for canola. The other two promoters, BnPro3-GUS and BnPro5-GUS (B. napus, promoter 3 and 5), exhibited GUS activity in various tissues. None of these two promoters expressed in embryo, however. These novel Brassica native promoters can be used to modify canola genes for various purposes.

scholarly journals Production of Restorer Lines from Segregating Progenies of Brassica napus L. Having Good Agronomic Value

2020 ◽  
Vol 8 (4) ◽  
pp. 400-409
Author(s):  
A. K. M. Aminul Islam ◽  
F. M. Era ◽  
N. K. Aminul Chowdhury
Keyword(s):  
Brassica Napus ◽  
Seed Yield ◽  
Seed Weight ◽  
Pollen Sterility ◽  
The Other ◽  
Restorer Line ◽  
Brassica Napus L ◽  
Maximum Value ◽  
Plant Fertility ◽  
Number Of Seeds

Forty four testcross progenies obtained from crossing between five CMS and forty one candidate lines were evaluated both in field and laboratory condition to identify candidate restorer line(s). Nineteen testcross progenies were recorded as fully fertile with 100% plant fertility by counting the number of fertile plants from the total number of plants per lines. On the other hand from pollen fertility test, 11 testcross progenies [206A × 001(6), 9905A × 030(2), 9905A × 027(6) (0.57), 206A × 37(1) (0.68), 9904A × 027(4) (0.83), 248A × 020(6) (1.08), 248A × 018 (1.12), 248A × 022 (1.13), 248A × 017 (1.58), 248A × 038(2) (1.96) and 248A × 001(6) (2.02)] were found with 0-2.02% pollen sterility that could be mentioned as fertile or restorer lines for making hybrids. Agronomic performances were also satisfactory for these selected test cross progenies. The genotype 248A × 017 took the shortest time (30.00) for first flowering as well as ripening followed by 248A × 007(1). The highest number of pods per plant was observed in the testcross progeny 248A × 022 (649.59) and the highest number of seeds per pod (30.33) in 248A × 020(6). For seed weight per plant, 206A × 001(6) was recorded with maximum value (0.08g) while the lowest (23.33) number of seeds per plant was found in this progeny. In case of seed yield per plant, 248A × 022 gave the highest yield (30.30). The seed yield of the progenies 248A × 017 (23.49), 9905A × 027(6) (20.39), 248A × 001(6) (17.26), 9904A × 027(4) (17.02) and 248A × 038(2) (16.53) were also in the highest level.

Effects of burial, stem portion and cultivar on the decomposition of canola straw

1999 ◽  
Vol 79 (1) ◽  
pp. 97-100 ◽  
Author(s):  
Peter V. Blenis ◽  
Pak S. Chow ◽  
Gary R. Stringam
Keyword(s):  
Brassica Napus ◽  
Key Words ◽  
Treatment Effects ◽  
Soil Surface ◽  
The Other ◽  
Brassica Napus L ◽  
Straw Decomposition ◽  
One Year ◽  
Surface Decomposition ◽  
Canola Straw

Buried canola straw underwent 1.6 to 2 times as much decomposition as straw on the soil surface. Decomposition of Brassica napus L. was slightly less than that of B. rapa L. and cultivar effects were small in one year and insignificant in the other. Because treatment effects varied between stems and roots, plant portion likely should be considered in decomposition studies. Key words: canola, straw decomposition, burial, cultivars

Variation in the pre-anthesis development of spring rape (Brassica napus L.)

1977 ◽  
Vol 28 (4) ◽  
pp. 597 ◽  
Author(s):  
N Thurling ◽  
LDV Das
Keyword(s):  
Growth Rate ◽  
Brassica Napus ◽  
Continuous Light ◽  
Early Flowering ◽  
The Other ◽  
Direct Effects ◽  
Brassica Napus L ◽  
Controlled Environments ◽  
Effects Of Temperature ◽  
Spring Rape

Fifteen cultivars of spring rape (Brassica napus L.) were each sown at four different times between April and October at Perth in either natural photoperiods or continuous light. Under natural photoperiods, flowering of the Japanese cultivars Chisaya and Isuzu was delayed in later sowings, but the flowering times of all other cultivars were advanced with each successive delay in sowing. When plants were grown under continuous light another two groups of cultivars could be recognized. One group comprised early-flowering Canadian cultivars which flowered later in the June sowing than in the other sowings. The other group comprised late-flowering European cultivars characterized by a slight delay in flowering in the last sowing. The direct effects of temperature on growth rate were more important than inductive responses in determining time of flowering in all cultivars except Chisaya and Isuzu, which had substantial vernalization requirements. Other cultivars such as Komet, Bronowski, Masoweicki and Norin 16 had low vernalization requirements which were largely masked by the increase in the rate of growth with higher temperatures in later sowings. In controlled environments, considerable variation in response to vernalization, temperature and photoperiod was detected between six spring rape cultivars. The early-flowering Canadian cultivars Target and Oro were the least responsive to vernalization and under continuous light did not respond at all. European and Japanese cultivars used here responded more markedly to vernalization, and their responses were greatly influenced by temperatures experienced after the completion of vernalization. Under continuous light and high temperature, the Japanese cultivars responded markedly to 4 weeks' vernalization which apparently saturated the system. The European cultivars Bronowski and Masoweicki, on the other hand, only responded moderately.

A comparative study of transgenic canola (Brassica napus L.) harboring either chimeric or native Chit42 genes against phytopathogenic fungi

2016 ◽  
Vol 25 (4) ◽  
pp. 358-366 ◽  
Author(s):  
Mahboobeh Ziaei ◽  
Mostafa Motallebi ◽  
Mohammad Reza Zamani ◽  
Nasim Zarin Panjeh ◽  
Zahra Moghaddassi Jahromi
Keyword(s):  
Brassica Napus ◽  
Comparative Study ◽  
Phytopathogenic Fungi ◽  
Brassica Napus L ◽  
Transgenic Canola

The efficiency of boron utilisation in canola

2001 ◽  
Vol 28 (11) ◽  
pp. 1109 ◽  
Author(s):  
James C. R. Stangoulis ◽  
Patrick H. Brown ◽  
Nacer Bellaloui ◽  
Robert J. Reid ◽  
Robin D. Graham
Keyword(s):  
Abiotic Stress ◽  
Brassica Napus ◽  
Boric Acid ◽  
Genotypic Variation ◽  
Plant Age ◽  
The Other ◽  
Brassica Napus L ◽  
Mature Leaves ◽  
The Impact ◽  
B Efficiency

Boron (B) deficiency is a common abiotic stress in many countries and is known to reduce canola yields. Growing B-efficient canola cultivars on deficient soils can reduce the impact of this deficiency, but to date little is known of the mechanisms associated with the efficiency trait. This work investigated the factors underlying the observed genotypic variation in the ability of different cultivars of canola (Brassica napus L.) to grow under low B supply. Previous studies have identified the cultivars Huashuang-2 and Dunkeld as B-efficient and the cultivar Barossa as B-inefficient. It was found that in Huashuang-2, efficiency was not related to uptake of B to the shoots, possibly indicating efficiency in B utilisation. A correlation was established between B efficiency and the ratio of the B concentration in the younger relative to the older leaves. Comparison of the distribution of B in young and old leaves under deficient and adequate B conditions showed that when B was limited, the concentration of B in the older leaves decreased with plant age, whereas when sufficient B was supplied, the concentration in these leaves continued to increase with age. The loss of B from mature leaves under deficient conditions suggested retranslocation to other tissues. Experiments in which boric acid enriched with 10 B was applied to mature leaves showed that in only one of the efficient cultivars, Huashuang-2, was B retranslocated to younger leaves. In the other two cultivars, the sink for the 10 B exported from the older leaves was not identified. It was concluded that there exist multiple mechanisms for B efficiency in canola.

The response of canola (Brassica napus L.) to tillage and fertiliser placement in contrasting environments in southern NSW

2003 ◽  
Vol 43 (11) ◽  
pp. 1323 ◽  
Author(s):  
P. J. Hocking ◽  
J. A. Mead ◽  
A. J. Good ◽  
S. M. Diffey
Keyword(s):  
Brassica Napus ◽  
Soil Degradation ◽  
Dry Matter ◽  
Soil Strength ◽  
The Other ◽  
Tillage Systems ◽  
Brassica Napus L ◽  
Land Preparation ◽  
No Till ◽  
Seed Yields

Land preparation for canola (oilseed rape; Brassica napus L.) by conventional cultivation can involve a number of workings, resulting in soil degradation and reduced crop growth. Minimum-tillage systems may help overcome these problems, but the placement of fertiliser at sowing must avoid chemical injury to germinating seed. The responses of canola cultivars to tillage and fertiliser placement were studied for 2 seasons at high (Breakfast Creek, 1997; Harden, 1998) and low (Ardlethan, 1997–98) rainfall sites. The tillage treatments were conventional cultivation, one-pass, and no-till (direct drill). The fertiliser treatments were 200 kg/ha 'starter' fertiliser (a�compound fertiliser supplying 30 kg N, 26 kg P and 22 kg S/ha) either placed with the seed, or broadcast, or banded to the side and 3 cm below the seed. In 1997 the canola was sown after wheat, and in 1998 after pasture. Plant establishment of all cultivars was reduced by 40–65% when fertiliser was placed with the seed; tillage treatment did not alter this response. Placing fertiliser with the seed reduced dry matter/m2 by up to 40% in plants at flowering, but by physiological maturity, there were no differences in dry matter/m2 due to fertiliser placement. Analysis of the combined seed yields for both years showed that although plants in the with-seed placement compensated by producing more seed/plant, this compensation was sufficient only at Breakfast Creek for yields to be comparable to those of the other fertiliser placements. Tillage had little effect on seed yields. In 1997, no-till yielded more than one-pass at Ardlethan, but in 1998 at Ardlethan no-till yielded less than the other tillage systems. Fertiliser placement and tillage had no effect on seed oil concentration and meal protein content. Cone penetrometer measurements (1998) showed no differences in soil strength between tillage treatments at Ardlethan; while at Harden, one-pass had less soil strength than the other tillage treatments. Crop water extraction was not affected by tillage at any site. It is concluded that a conservation-farming system involving no-till or one-pass tillage, and separation of seed and fertiliser has the potential for producing high yielding canola crops, reducing the risk of soil degradation, as well as saving time and land-preparation costs.

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