The improving effects of biotin on hepatic histopathology and related apolipoprotein mRNA expression in laying hens with fatty liver hemorrhagic syndrome

2020 ◽  
Vol 100 (3) ◽  
pp. 494-501
Author(s):  
Jiamei Huang ◽  
Guyue Li ◽  
Hongfeng Cao ◽  
Fei Yang ◽  
Chenghong Xing ◽  
...  
Keyword(s):  
Treatment Group ◽  
Fatty Liver ◽  
Mrna Expression ◽  
Egg Production ◽  
Laying Hens ◽  
Mrna Level ◽  
Basal Diet ◽  
High Energy ◽  
Control Group ◽  
Mrna Levels

Fatty liver hemorrhagic syndrome (FLHS) is a metabolic disease mostly observed in laying hens that causes an economic toll on the poultry industry. To investigate the improving effects of biotin on FLHS in laying hens, a total of 135 Hy-Line Brown layers of 300-d-old were randomly divided into three groups and treated for 60 d. The hens from these three groups were fed with different diets: control group (the basal diet), pathology group [high-energy-low-protein diet (HELP)], and treatment group (HELP containing a biotin dosage of 0.3 mg kg−1). The results showed that the mRNA expression level of apolipoprotein A I (apoA I) in pathology group significantly (P < 0.01) decreased on day 60 compared with the control group, while the mRNA level of apolipoprotein B100 (apoB100) increased significantly in pathology group on day 30, whereas it decreased significantly on day 60 (P < 0.05). Significantly increased mRNA levels of apoA I and apoB100 were observed in treatment group compared with the pathology group on days 30 and 60 (P < 0.05 or P < 0.01). These results indicated that biotin could effectively alleviate the pathological changes and abnormal expression of apoA I and apoB100 induced by FLHS, which might closely relate to the ability of biotin to promote egg production.

scholarly journals Effects of Dietary Inclusion of Dry Hydrastis canadensis on Laying Performance, Egg Quality, Serum Biochemical Parameters and Cecal Microbiota in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1381
Author(s):  
Tzuen-Rong J Tzeng ◽  
Tzu-Yu Liu ◽  
Chiao-Wei Lin ◽  
Pei-En Chang ◽  
Pei-Xin Liao ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Egg Production ◽  
Laying Hens ◽  
Basal Diet ◽  
Response To Treatment ◽  
Control Group ◽  
Hydrastis Canadensis ◽  
Laying Performance ◽  
Cecal Microbiota ◽  
Dietary Treatments

Alternative growth promoters are able to not only effectively replace the traditional use of antibiotics but also provide additional health benefits for livestock and reduce food safety concerns. This study investigated the effects of dry Hydrastis canadensis on the laying performance and fecal microbial community of laying hens. Twenty-four Lohmann (LSL, white layer strain) hens were reared from 40 to 48 weeks of age and randomly allotted to four dietary treatments (six birds/treatment). The dietary treatments comprised a basal diet with no treatment as control, a basal diet plus 0.6% powder of dry Hydrastis canadensis roots (R) or leaves (L), and a basal diet plus 0.6% powder of a mixture of dry Hydrastis canadensis roots and leaves (1:1, LR). No mortality was observed in the whole experimental period. The results indicated that albumen height in the LR group was significantly greater than that in the control group. The diet supplemented with Hydrastis canadensis had no significant effects on egg production rate, egg weight, eggshell strength, eggshell thickness, Haugh unit, or yolk height during the whole experimental phase. However, principal coordinate analysis, comparative heat map analysis, and cluster dendrogram analysis of cecal microbiota showed distinct clusters among the groups treated with Hydrastis canadensis and the control group. Regarding blood biochemical parameters, serum cholesterol levels were significantly lower in all Hydrastis canadensis-treated groups compared with those in the control group. Moreover, serum low-density lipoprotein levels were lower in hens supplemented with the leaf of Hydrastis canadensis. The abundances of the phyla Fusobacteria and Kiritimatiellaeota were increased (p < 0.05) in laying hens fed with 0.6% Hydrastis canadensis leaves, whereas the abundance of the phylum Firmicutes in cecum digesta decreased in response to treatment with Hydrastis canadensis roots and leaves. The relative abundance of the Fusobacterium genus was higher in the LR group compared with that in the control. On the contrary, we found a different trend in the Synergistes genus. The potential influences of these microbiota on the performance of laying hens were discussed. The results demonstrate that Hydrastis canadensis can improve the egg albumen height and modulate the cecum digesta microbiota composition of laying hens.

scholarly journals Effects of feed supplementation with manganese from its different sources on performance and egg parameters of laying hens

2014 ◽  
Vol 59 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
K. Venglovská ◽  
Ľ. Grešáková ◽  
I. Plachá ◽  
M. Ryzner ◽  
K. Čobanová
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Protein Hydrolysate ◽  
Basal Diet ◽  
Egg Yolk ◽  
Control Group ◽  
Feed Supplementation ◽  
Positive Effects ◽  
Dietary Treatments

The objective of this study was to compare the effects of feed supplementation of laying hens with manganese from its inorganic and organic sources on performance and some parameters of egg quality. Ninety-six hens at 20 weeks of age were randomly allocated to 4 dietary treatments, each consisting of 6 replicates (4 birds per replicate). The control group was fed unsupplemented basal diet (BD) with only natural background Mn level of 46.4 mg/kg feed. For the three experimental treatments, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Pro) or Mn-chelate of glycine hydrate (Mn-Gly). After 8 weeks of dietary treatments the egg production, egg weight, feed intake, and feed efficiency were not affected by dietary treatments. Regardless of the sources, Mn supplementation to feed resulted in significantly decreased percentages of cracked eggs compared to the unsupplemented control group. The thickness, weight, proportion, and index of eggshell were significantly elevated in all groups supplemented with Mn. The intake of Mn-Gly resulted in considerably increased Mn deposition in egg yolk compared to the control eggs. In the control and Mn-sulphate groups yolk malondialdehyde (MDA) started to increase after 20 and 30 days of egg storage respectively, whereas in eggs from hens given organic Mn-sources this parameter was not affected up to 40 days. Although there were no significant differences in MDA values between the treatments until 20 days of storage, the Mn-sulphate group showed significantly higher MDA concentration in yolks compared to the control group after 30 days of storage. These results demonstrate that supplementation of hens&rsquo; diet with Mn has positive effects on eggshell quality. Feed supplementation with Mn from organic sources appears to be more effective in preventing yolk lipid oxidation during cold storage of eggs than that from Mn-sulphate. &nbsp;

scholarly journals The effect of dietary Jerusalem artichoke (Helianthus tuberosus L.) on performance, egg quality characteristics and egg cholesterol content in laying hens

2011 ◽  
Vol 51 (No. 8) ◽  
pp. 349-354 ◽  
Author(s):  
G. Yildiz ◽  
P. Sacakli ◽  
T. Gungor
Keyword(s):  
Treatment Group ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Jerusalem Artichoke ◽  
Live Weight ◽  
Cholesterol Content ◽  
Quality Characteristics ◽  
Metabolizable Energy ◽  
Control Group

This research was carried out to determine the effect of Jerusalem artichoke (JA) with or without 5, 10% vetch (V) supplementation on performance, egg quality characteristics and egg cholesterol content. In the study, seventy-five 25 weeks-old commercial white laying hens were randomly divided into one control and 4 treatment groups each containing 15 hens. Control group was fed basal diet without JA or V. Treatment group 1, 2, 3 and 4 were fed diets containing 5% V, 5% JA, 5% JA + 5% V and 10% JA + 10% V, respectively. The feeding period lasted 16 weeks. In the study, it was determined that dried-ground JA contained dry matter, crude protein, ether extract, crude fibre, crude ash, inulin and metabolizable energy (ME) at the level of 93.30, 10.02, 0.36, 5.64, 9.05, 15.80% and 3 060 kcal/kg, respectively. At the end of the study, live weight was lower by 4.36&ndash;10.09% in the treatment group 10% JA + 10% V compared with the other groups, but feed efficiency was improved in this group. There were statistically significant differences between the groups in egg quality characteristics (P &lt; 0.05, P &lt; 0.001). Egg production was not affected by supplementation of 5, 10% JA with or without 5, 10% V supplementation. Egg yolk cholesterol and total cholesterol content were not different in the groups (P &gt; 0.05). As a result, the addition of JA with or without V has no adverse effect on performance and egg quality in hens. &nbsp;

scholarly journals mRNA Expression of CYP2E1, CYP2A19, CYP1A2, HSD3B, SULT1A1 and SULT2A1 genes in surgically castrated, immunologically castrated, entire male and female pigs and correlation with androstenone, skatole, indole and Improvac-specific antibody levels

2019 ◽  
Vol 64 (No. 2) ◽  
pp. 89-97
Author(s):  
A. Kubešová ◽  
K. Šťastný ◽  
M. Faldyna ◽  
Z. Sládek ◽  
I. Steinhauserová ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Mrna Level ◽  
Boar Taint ◽  
Control Group ◽  
Mrna Levels ◽  
Large White ◽  
Specific Antibodies ◽  
Significant Difference ◽  
Entire Male

This study aimed to obtain a comprehensive look at the influence of castration on mRNA expression of the genes CYP2E1, CYP1A2, CYP2A19, HSD3B, SULT2A1 and SULT1A1 and their correlation with boar taint compounds (androstenone, skatole and indole) and Improvac-specific antibodies in a Czech commercial hybrid (Large White × Landrace (sow) × Duroc (boar)). Pigs were divided into groups of entire male pigs (NC), pigs castrated surgically (SC), pigs immunologically castrated and slaughtered 8 weeks (IM8) or 15 weeks (IM15) after the second dose of Improvac, and gilts (GI). Hepatic mRNA expression, measured by quantitative real-time polymerase chain reaction, differed significantly between the control group (entire male pigs) and all groups of interest for CYP2E1, CYP1A2 and CYP2A19. The mRNA level of the HSD3B gene differed significantly between the control group and the IM8, IM15 and GI groups. SULT1A1 gene expression was significantly different between the control group and the SC, IM8 and GI. In the case of SULT2A1, a significant difference was observed only between the control group and IM8 pigs. For all genes and treatment groups described above, expression was increased relative to the control. Significant differences for Improvac-specific antibodies between IM8 and IM15 groups were observed, indicating decrease of antibodies over time. Moreover, negative correlations between androstenone and mRNA levels of CYP2A19, CYP2E1 and SULT1A1 suggest that gene expression is suppressed.

scholarly journals Effect of Date Palm Pollen Supplementation on the Egg Production, Ovarian Follicles Development, Hematological Variables and Hormonal Profile of Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 69
Author(s):  
Mohamed Saleh ◽  
Dariusz Kokoszyński ◽  
Mohamed Abd-Allah Mousa ◽  
Ahmed Abdel-Kareem Abuoghaba
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Ovarian Follicle ◽  
Basal Diet ◽  
Ovarian Follicles ◽  
Control Group ◽  
Egg Mass ◽  
Egg Weight ◽  
Hormonal Profile ◽  
Ovarian Follicle Development

This experiment studied the effect of DPP supplementation in the laying hens’ diet on the ovarian follicle development, hematological variables and hormonal profile of laying hens. Eighty-four, 78-week-old, Lohman LSL hybrids layers were equally divided into four groups (4 groups × 7 replicates × 3 hens); hens in the 1st group were fed a basal diet (control), while those in the 2nd, 3rd and 4th groups, were fed on the same diet and supplemented with 1.25, 2.5 and 5.0 g DPP/kg diet. The results showed that the egg weight, egg mass and laying rate of laying hens treated with DPP levels were significantly increased compared to those of the hens in the control group. Egg weight, egg surface area, albumen quality and percentage of the yolk in treated hens significantly increased compared with controls. The increased DPP levels in laying hens‘ diet significantly (p < 0.05) increased WBC, Hb and TAC, while heterophil/lymphocyte (H/L ratio) significantly decreased. Additionally, the concentrations of FSH and LH and the weights of ovary and oviduct in the treated hens significantly (p < 0.05) increased compared with the control. In conclusion, the DPP supplementation in the hen diet significantly improved egg production, EW, H/L ratio, ovarian follicles, FSH and LH hormones concentrations.

Changes of Metallothionein 1 and 3 mRNA Levels with Age in Brain of Senescence-Accelerated Mice and The Effects of Acupuncture

2006 ◽  
Vol 34 (03) ◽  
pp. 435-447 ◽  
Author(s):  
Tingyi Wen ◽  
Xiaonong Fan ◽  
Mingchun Li ◽  
Jingxian Han ◽  
Xuemin Shi ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Mrna Level ◽  
Zinc Ion ◽  
Control Group ◽  
Mrna Levels ◽  
Expression Levels ◽  
Age Related ◽  
Zigzag Pattern ◽  
Effects Of Aging ◽  
Senescence Accelerated Mice

The effects of aging and acupuncture on brain MT1 and MT3 mRNA levels in senescence-accelerated mice (SAMP10) and accelerated senescence resistant mice (SAMR1) were analyzed by Northern blot analysis. Both MT1 and MT3 mRNA levels in SAMR1 were increased significantly from birth to month 4 and decreased gradually thereafter. In SAMP10, the MT3 mRNA level followed the same pattern as in SAMR1 before month 4, then decreased from month 4 to 6, but was over-expressed and exceeded the previous level at month 8. The MT1 mRNA expression in SAMP10 showed a zigzag pattern. Of two groups of SAMP10 mice treated with acupuncture, the Xingnao group (PC6 and Du26 as acupoints) and the Zibuganshen group (BL18 and BL23 as acupoints), both showed a higher MT1 mRNA level and a lower MT3 mRNA level than the age-matched control group. Meanwhile, in both of the acupuncture groups, the ratios of MT3 to MT1 were down-regulated to the normal range. Overall, these results suggested that over-expression of MT3 mRNA and the increase in MT3 to MT1 ratios in SAMP10 were correlated with aging, and could be an important physiological and pathological event in the aging process. Acupuncture altered the expression levels of MT1 and MT3 mRNA and differences between the effects of the two stimulated acupoints were seen. Therefore, maintenance of the balance between MTs mRNA expression and correct MTs concentrations is crucial for brain-endocrine-immune response and normal aging. Acupuncture could improve zinc ion bioavailability, by maintaining the balance between MT1 and MT3 mRNA expression levels and might explain one of the mechanisms by which acupuncture treatments defer aging and treat some age-related neurodegenerative diseases.

CHFR Promoter Hypermethylation and Reduced CHFR mRNA Expression in Ovarian Cancer

2009 ◽  
Vol 24 (2) ◽  
pp. 83-89 ◽  
Author(s):  
Ying Gao ◽  
Ge Lou ◽  
Guang-Mei Zhang ◽  
Xi-Wen Sun ◽  
Yu-Yan Ma ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Mrna Expression ◽  
Mrna Level ◽  
Promoter Hypermethylation ◽  
Ovarian Tumors ◽  
Control Group ◽  
Mrna Levels ◽  
Ovarian Cancers ◽  
Cancer Tissues ◽  
Normal Controls

Background Ovarian cancer is one of the most common cancers and can be treated with microtubule-targeting drugs. Checkpoint with forkhead and ring finger domains (CHFR) is a protein implicated in cancer sensitivity to microtubule-targeting drugs. Whereas CHFR downregulation, often with CHFR promoter hypermethylation, has been identified in a large number of tumor types, it has not been in ovarian cancer. We therefore searched for CHFR downregulation in primary ovarian tumors. Methods Fresh ovarian cancer tissues from 53 patients (test) and normal ovarian tissues from 21 patients (control) were tested for CHFR promoter hypermethylation and CHFR mRNA levels. Results The CHFR promoter was hypermethylated in 20.75% (11/53) of the ovarian cancers and none (0/21) of the normal controls. The normal controls had a mean mRNA level of 1.89 relative fluorescence units (RFU) with a range of 0.04–24.78 RFU. The cancer tissues had a mean mRNA level of 0.77 RFU with a range of 0.00–68.75 RFU. The median value of the cancer group was significantly lower than that of the control group (p=0.0067). Those cancer samples that had hypermethylated CHFR promoters also had low (n=3) or undetectable (n=8) CHFR mRNA levels. Conclusions In contrast to previous reports, we found that alterations in CHFR mRNA and CHFR methylation can be frequently found in ovarian cancers. CHFR hypermethylation was strongly associated with the loss of CHFR mRNA expression. CHFR downregulation in ovarian tumors may be clinically relevant as a staging biomarker, as an indicator of sensitivity to microtubule-targeting drugs, and as a future drug target.

Probiotic and prebiotic supplementation improving the production performance and immune characteristics of laying hens

2017 ◽  
Author(s):  
Abhed Pandey ◽  
Avtar Singh ◽  
Shanthanagouda Admane Holeyappa ◽  
Harsimranjit Kaur
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Basal Diet ◽  
Production Performance ◽  
Control Group ◽  
Egg Mass ◽  
Feeding Trial ◽  
Treatment Groups ◽  
Cell Mediated Immune Response ◽  
Bacillus Spp

A 16 week feeding trial was conducted with 140 White Leghorn layers of 22-23±0.08 weeks of age which were distributed randomly into seven experimental groups, having 4 replicates with 5 birds in each. The laying hens of control group (T1) were fed a basal diet (CP 18.04%), while in treatment groups T2, T3, T4 basal diet was supplemented with probiotic (containing 5×108 cfu/g of Lactobacillus fermentum, 1×109 cfu/g of Bacillus spp., 1×109cfu/g of Saccharomyces cerevisiae) at levels of 0.5g, 1.0 g and 2.0 g /kg feed, respectively, while in T5, T6, and T7 0.5g, 1.0g and 2.0g/kg feed of prebiotic (mannonoligosacchride of Saccharomyces cell wall-47g, formic acid-32g) was supplemented, respectively. The percent hen day egg production and feed efficiency (kg/dozen eggs and kg/kg egg mass) were improved (P less than 0.05) in probiotic and prebiotic incorporated diets. The mRNA expression levels of Toll like receptors (TLRs) TLR2 and TLR4 was increased (P less than 0.05) when graded levels of the probiotic and prebiotic were supplemented, while, TLR7 did not show any significant change. Thus, it can be inferred that the inclusion of the probiotic and prebiotic could enhance the production performance and immune status of birds by augmenting the T cell mediated immune response.

scholarly journals Effects of fermented Caragana korshinskii on the intramuscular fat content and expression of FABP3, UBE3C, ADRB3, LIPE, and SCD in different muscles of Tan sheep

2020 ◽  
Vol 65 (No. 4) ◽  
pp. 145-152
Author(s):  
Xiaochun Xu ◽  
Tingda Liu ◽  
Shanshan Fan ◽  
Wenping Ma ◽  
Wenjuan Chen ◽  
...  
Keyword(s):  
Mrna Level ◽  
Basal Diet ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Fat Deposition ◽  
Control Group ◽  
Mrna Levels ◽  
Caragana Korshinskii ◽  
Tan Sheep ◽  
Intramuscular Fat Content

The aim of this study was to investigate the effects of fermented Caragana korshinskii on the intramuscular fat content and varied expression of the intramuscular fat deposition-related genes FABP3, UBE3C, ADRB3, LIPE, and SCD among four muscle tissues (m. psoas, gluteus, quadriceps, and supraspinatus) of Tan sheep. Twenty-eight male animals of similar age (270 ± 10 days) and weight (24.6 ± 1.06 kg) were randomly divided into a control group (fed the basal diet) and an experimental group (fed the same diet except 10% of corn stalks were replaced with fermented C. korshinskii). Soxhlet petroleum-ether extraction and quantitative real-time PCR were applied to evaluate the fat content and gene expression in tissues, respectively. We observed a significant improvement (P &lt; 0.05) in the intramuscular fat contents in the m. gluteus and supraspinatus of treated sheep compared to those of non treated sheep. The FABP3 mRNA level was markedly higher (P &lt; 0.05) in the m. quadriceps and supraspinatus of treated sheep than in the control sheep. UBE3C mRNA levels were significantly decreased in the m. gluteus, quadriceps, and supraspinatus (P &lt; 0.05) of treated sheep compared with those of the control sheep. ADRB3 mRNA levels were significantly lower (P &lt; 0.05) in the m. psoas, gluteus, and supraspinatus of sheep fed fermented C. korshinskii than in the control group, whereas LIPE mRNA levels were significantly increased (P &lt; 0.05) in the m. gluteus and quadriceps of sheep fed fermented C. korshinskii. The SCD mRNA levels in m. psoas, quadriceps, and supraspinatus of sheep fed fermented C. korshinskii were significantly higher than those of the control group (P &lt; 0.05). Our results indicated that fermented C. korshinskii could partially replace the roughage used in Tan sheep feed, and its substitution affected the intramuscular fat content and altered the expression of intramuscular fat deposition-related genes. The present study lays a solid foundation for further exploring the utilization of C. korshinskii in ruminant husbandry.

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