Expression patterns of PPARγ2, PGC-1α, and MEF2C and their association with intramuscular fat content and skeletal muscle tenderness of crossbred Simmental bulls

2019 ◽  
Vol 99 (2) ◽  
pp. 367-376
Author(s):  
Li-Qin Yang ◽  
Jian Li ◽  
Chun Wang ◽  
Qiu-Ying Wu ◽  
Xuan-Yu Chen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Shear Force ◽  
Muscle Development ◽  
Expression Patterns ◽  
Intramuscular Fat ◽  
Skeletal Muscle Development ◽  
Muscle Tenderness ◽  
Imf Content ◽  
Intramuscular Fat Content

PPARγ2, PGC-1α, and MEF2C play an important role in skeletal muscle development and fat deposition. This study aimed to determine their mRNA expression levels in longissimus dorsi (Ld), semitendinosus (Se), and soleus (Sol) muscles of crossbred Simmental bulls and estimate their association with intramuscular fat (IMF) content and meat shear force (MSF). We measured the muscle fiber (MF) density and area, IMF content, and MSF of 6-, 12-, and 36-mo-old bulls. We found that the expression patterns differed with age: the PPARγ2 expression in the three muscles of 36-mo-old bulls was greater than that in the muscles of 6- and 12-mo-old bulls (P < 0.05). Furthermore, PGC-1α expression in Sol of 36-mo-old and MEF2C expression in Ld of 12-mo-old bulls were higher than those in the respective muscles of 6- and 12-mo-old bulls, and 6- and 36-mo-old bulls, respectively (P < 0.05). The MF area, IMF content, and MSF increased with age (P < 0.05). The PPARγ2 mRNA expression in Ld, Se, and Sol was positively correlated with MF area and IMF content (P < 0.05) and negatively correlated with MF density (P < 0.05). Thus, PPARγ2 might be a candidate marker, which is positively correlated with IMF content and MF area.

scholarly journals Chromatin Interaction Responds to Breast Muscle Development and Intramuscular Fat Deposition Between Chinese Indigenous Chicken and Fast-Growing Broiler

2021 ◽  
Vol 9 ◽  
Author(s):  
Weihua Tian ◽  
Zhang Wang ◽  
Dandan Wang ◽  
Yihao Zhi ◽  
Jiajia Dong ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Intramuscular Fat ◽  
Breast Muscle ◽  
Chromatin Interaction ◽  
Chicken Breast ◽  
Meat Production ◽  
Skeletal Muscle Development ◽  
Significant Difference ◽  
Imf Content

Skeletal muscle development and intramuscular fat (IMF) content, which positively contribute to meat production and quality, are regulated by precisely orchestrated processes. However, changes in three-dimensional chromatin structure and interaction, a newly emerged mediator of gene expression, during the skeletal muscle development and IMF deposition have remained unclear. In the present study, we analyzed the differences in muscle development and IMF content between one-day-old commercial Arbor Acres broiler (AA) and Chinese indigenous Lushi blue-shelled-egg chicken (LS) and performed Hi-C analysis on their breast muscles. Our results indicated that significantly higher IMF content, however remarkably lower muscle fiber diameter was detected in breast muscle of LS chicken compared to that of AA broiler. The chromatin intra-interaction was prior to inter-interaction in both AA and LS chicken, and chromatin inter-interaction was heavily focused on the small and gene-rich chromosomes. For genomic compartmentalization, no significant difference in the number of B type compartments was found, but AA had more A type compartments versus LS. The A/B compartment switching of AA versus LS showed more A to B switching than B to A switching. There were no significant differences in the average sizes and distributions of topologically associating domains (TAD). Additionally, approximately 50% of TAD boundaries were overlapping. The reforming and disappearing events of TAD boundaries were identified between AA and LS chicken breast muscles. Among these, the HMGCR gene was located in the TAD-boundary regions in AA broilers, but in TAD-interior regions in LS chickens, and the IGF2BP3 gene was located in the AA-unique TAD boundaries. Both HMGCR and IGF2BP3 genes exhibited increased mRNA expression in one-day-old AA broiler breast muscles. It was demonstrated that the IGF2BP3 and HMGCR genes regulated by TAD boundary sliding were potential biomarkers for chicken breast muscle development and IMF deposition. Our data not only provide a valuable understanding of higher-order chromatin dynamics during muscle development and lipid accumulation but also reveal new insights into the regulatory mechanisms of muscle development and IMF deposition in chicken.

511 Temporal Expression Patterns of Twelve Genes during Fetal and Postnatal Skeletal Muscle Development in Pigs.

2018 ◽  
Vol 96 (suppl_2) ◽  
pp. 272-273
Author(s):  
B A Wolfer ◽  
K R Daza ◽  
D Velez-Irizarry ◽  
N E Raney ◽  
V D Rilington ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Expression Patterns ◽  
Skeletal Muscle Development ◽  
Temporal Expression

scholarly journals Potential role of miR-155-5p in fat deposition and skeletal muscle development of chicken

2020 ◽  
Vol 40 (6) ◽  
Author(s):  
Sifan Xu ◽  
Yang Chang ◽  
Guanxian Wu ◽  
Wanting Zhang ◽  
Chaolai Man
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Target Genes ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Fat Deposition ◽  
Skeletal Muscle Development ◽  
Stem Loop ◽  
Related Target ◽  
Kegg Pathways

Abstract miR-155 has multiple functions in many physiological and pathological processes. However, little is known about the expression characteristics of avian miR-155. In the present study, partial pri-miR-155 sequences were cloned from AA+ broiler, Sanhuang broiler and Hy-Line Brown layer, respectively. Stem–loop qRT-PCR was performed to detect the miR-155-5p spatiotemporal expression profiles of each chicken breed, and the target genes of miR-155-5p were predicted in Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The results showed that the partial pri-miR-155 sequences of different breeds of chicken were high conserved. The expression patterns of miR-155-5p between broiler and layer were basically similar, and miR-155-5p was expressed highly in immune related tissues (spleen, thymus and bursa). In the same old chicken (14 days old), miR-155-5p expression activity of fat tissue all had higher level in the three chicken breeds, but the expression activities in skeletal muscle of broilers were significantly lower than that of layer (P&lt;0.05). In different development stages of Hy-Line Brown layer, miR-155-5p expression activities in skeletal muscle of 14-day-old and 10-month-old layers were significantly lower than that of 24-month-old layer (P&lt;0.05). Fat related target genes (ACOX1, ACOT7, FADS1, SCD and HSD17B12) and skeletal muscle related target genes (CCNT2, DMD, CFL2, MAPK14, FLNB, ZBTB18 and CDK5) of miR-155-5p were predicted, respectively. The results indicate that miR-155-5p may be an important factor inhibiting the fat deposition and skeletal muscle development in chicken.

scholarly journals MicroRNA expression patterns in post-natal mouse skeletal muscle development

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Séverine Lamon ◽  
Evelyn Zacharewicz ◽  
Lauren C. Butchart ◽  
Liliana Orellana ◽  
Jasmine Mikovic ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Expression Patterns ◽  
Microrna Expression ◽  
Skeletal Muscle Development ◽  
Mouse Skeletal Muscle

scholarly journals Phenotypic Resemblance to Neuropsychiatric Disorder and Altered mRNA Profiles in Cortex and Hippocampus Underlying IL15Rα Knockout

2021 ◽  
Vol 14 ◽  
Author(s):  
Yi He ◽  
Yuxin Yu ◽  
Yanan Li ◽  
Weicheng Duan ◽  
Zuoli Sun ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Expression Patterns ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis ◽  
Control Group ◽  
Rna Seq ◽  
Skeletal Muscle Development ◽  
Altered Expression ◽  
Y Maze

BackgroundPrevious studies of the functions of IL15Rα have been limited to immune activities and skeletal muscle development. Immunological factors have been identified as one of the multiple causes of psychosis, and neurological symptoms have been described in IL15Rα knockout (KO) mice. Seeking to explore possible mechanisms for this in the IL15Rα–/– mouse brain, we analyzed gene expression patterns in the cortex and hippocampus using the RNA-seq technique.MethodsIL15Rα KO mice were generated and littermate wildtype (WT) mice were used as a control group. A Y-maze was used to assess behavior differences between the two groups. The cortex and hippocampus of 3-month-old male mice were prepared and RNA-seq and transcriptome analysis were performed by gene set enrichment analysis (GSEA).ResultsCompared with the WT group, IL15Rα KO animals showed higher speed in the novel arm and more entrance frequency in the old arm in the Y-maze experiment. GSEA indicated that 18 pathways were downregulated and 13 pathways upregulated in both cortex and hippocampus from the GO, KEGG, and Hallmark gene sets. The downregulated pathways formed three clusters: respiratory chain and electron transport, regulation of steroid process, and skeletal muscle development.ConclusionIL15Rα KO mice exhibit altered expression of multiple pathways, which could affect many functions of the brain. Lipid biosynthesis and metabolism in the central nervous system (CNS) should be investigated to provide insights into the effect of IL15Rα on psychosis in this murine model.

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