511 Temporal Expression Patterns of Twelve Genes during Fetal and Postnatal Skeletal Muscle Development in Pigs.

2018 ◽  
Vol 96 (suppl_2) ◽  
pp. 272-273
Author(s):  
B A Wolfer ◽  
K R Daza ◽  
D Velez-Irizarry ◽  
N E Raney ◽  
V D Rilington ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Expression Patterns ◽  
Skeletal Muscle Development ◽  
Temporal Expression

scholarly journals Potential role of miR-155-5p in fat deposition and skeletal muscle development of chicken

2020 ◽  
Vol 40 (6) ◽  
Author(s):  
Sifan Xu ◽  
Yang Chang ◽  
Guanxian Wu ◽  
Wanting Zhang ◽  
Chaolai Man
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Target Genes ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Fat Deposition ◽  
Skeletal Muscle Development ◽  
Stem Loop ◽  
Related Target ◽  
Kegg Pathways

Abstract miR-155 has multiple functions in many physiological and pathological processes. However, little is known about the expression characteristics of avian miR-155. In the present study, partial pri-miR-155 sequences were cloned from AA+ broiler, Sanhuang broiler and Hy-Line Brown layer, respectively. Stem–loop qRT-PCR was performed to detect the miR-155-5p spatiotemporal expression profiles of each chicken breed, and the target genes of miR-155-5p were predicted in Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The results showed that the partial pri-miR-155 sequences of different breeds of chicken were high conserved. The expression patterns of miR-155-5p between broiler and layer were basically similar, and miR-155-5p was expressed highly in immune related tissues (spleen, thymus and bursa). In the same old chicken (14 days old), miR-155-5p expression activity of fat tissue all had higher level in the three chicken breeds, but the expression activities in skeletal muscle of broilers were significantly lower than that of layer (P<0.05). In different development stages of Hy-Line Brown layer, miR-155-5p expression activities in skeletal muscle of 14-day-old and 10-month-old layers were significantly lower than that of 24-month-old layer (P<0.05). Fat related target genes (ACOX1, ACOT7, FADS1, SCD and HSD17B12) and skeletal muscle related target genes (CCNT2, DMD, CFL2, MAPK14, FLNB, ZBTB18 and CDK5) of miR-155-5p were predicted, respectively. The results indicate that miR-155-5p may be an important factor inhibiting the fat deposition and skeletal muscle development in chicken.

scholarly journals MicroRNA expression patterns in post-natal mouse skeletal muscle development

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Séverine Lamon ◽  
Evelyn Zacharewicz ◽  
Lauren C. Butchart ◽  
Liliana Orellana ◽  
Jasmine Mikovic ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Expression Patterns ◽  
Microrna Expression ◽  
Skeletal Muscle Development ◽  
Mouse Skeletal Muscle

Expression patterns of PPARγ2, PGC-1α, and MEF2C and their association with intramuscular fat content and skeletal muscle tenderness of crossbred Simmental bulls

2019 ◽  
Vol 99 (2) ◽  
pp. 367-376
Author(s):  
Li-Qin Yang ◽  
Jian Li ◽  
Chun Wang ◽  
Qiu-Ying Wu ◽  
Xuan-Yu Chen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Shear Force ◽  
Muscle Development ◽  
Expression Patterns ◽  
Intramuscular Fat ◽  
Skeletal Muscle Development ◽  
Muscle Tenderness ◽  
Imf Content ◽  
Intramuscular Fat Content

PPARγ2, PGC-1α, and MEF2C play an important role in skeletal muscle development and fat deposition. This study aimed to determine their mRNA expression levels in longissimus dorsi (Ld), semitendinosus (Se), and soleus (Sol) muscles of crossbred Simmental bulls and estimate their association with intramuscular fat (IMF) content and meat shear force (MSF). We measured the muscle fiber (MF) density and area, IMF content, and MSF of 6-, 12-, and 36-mo-old bulls. We found that the expression patterns differed with age: the PPARγ2 expression in the three muscles of 36-mo-old bulls was greater than that in the muscles of 6- and 12-mo-old bulls (P < 0.05). Furthermore, PGC-1α expression in Sol of 36-mo-old and MEF2C expression in Ld of 12-mo-old bulls were higher than those in the respective muscles of 6- and 12-mo-old bulls, and 6- and 36-mo-old bulls, respectively (P < 0.05). The MF area, IMF content, and MSF increased with age (P < 0.05). The PPARγ2 mRNA expression in Ld, Se, and Sol was positively correlated with MF area and IMF content (P < 0.05) and negatively correlated with MF density (P < 0.05). Thus, PPARγ2 might be a candidate marker, which is positively correlated with IMF content and MF area.

scholarly journals Phenotypic Resemblance to Neuropsychiatric Disorder and Altered mRNA Profiles in Cortex and Hippocampus Underlying IL15Rα Knockout

2021 ◽  
Vol 14 ◽  
Author(s):  
Yi He ◽  
Yuxin Yu ◽  
Yanan Li ◽  
Weicheng Duan ◽  
Zuoli Sun ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Expression Patterns ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis ◽  
Control Group ◽  
Rna Seq ◽  
Skeletal Muscle Development ◽  
Altered Expression ◽  
Y Maze

BackgroundPrevious studies of the functions of IL15Rα have been limited to immune activities and skeletal muscle development. Immunological factors have been identified as one of the multiple causes of psychosis, and neurological symptoms have been described in IL15Rα knockout (KO) mice. Seeking to explore possible mechanisms for this in the IL15Rα–/– mouse brain, we analyzed gene expression patterns in the cortex and hippocampus using the RNA-seq technique.MethodsIL15Rα KO mice were generated and littermate wildtype (WT) mice were used as a control group. A Y-maze was used to assess behavior differences between the two groups. The cortex and hippocampus of 3-month-old male mice were prepared and RNA-seq and transcriptome analysis were performed by gene set enrichment analysis (GSEA).ResultsCompared with the WT group, IL15Rα KO animals showed higher speed in the novel arm and more entrance frequency in the old arm in the Y-maze experiment. GSEA indicated that 18 pathways were downregulated and 13 pathways upregulated in both cortex and hippocampus from the GO, KEGG, and Hallmark gene sets. The downregulated pathways formed three clusters: respiratory chain and electron transport, regulation of steroid process, and skeletal muscle development.ConclusionIL15Rα KO mice exhibit altered expression of multiple pathways, which could affect many functions of the brain. Lipid biosynthesis and metabolism in the central nervous system (CNS) should be investigated to provide insights into the effect of IL15Rα on psychosis in this murine model.

scholarly journals The Key Lnc (RNA)s in Cardiac and Skeletal Muscle Development, Regeneration, and Disease

2021 ◽  
Vol 8 (8) ◽  
pp. 84
Author(s):  
Amanda Pinheiro ◽  
Francisco J. Naya
Keyword(s):  
Skeletal Muscle ◽  
Mammalian Cells ◽  
Molecular Mechanisms ◽  
Muscle Development ◽  
Striated Muscle ◽  
Expression Patterns ◽  
Regulation Of Gene Expression ◽  
Therapeutic Interventions ◽  
Skeletal Muscle Development ◽  
Non Coding Rnas

Non-coding RNAs (ncRNAs) play a key role in the regulation of transcriptional and epigenetic activity in mammalian cells. Comprehensive analysis of these ncRNAs has revealed sophisticated gene regulatory mechanisms which finely tune the proper gene output required for cellular homeostasis, proliferation, and differentiation. However, this elaborate circuitry has also made it vulnerable to perturbations that often result in disease. Among the many types of ncRNAs, long non-coding RNAs (lncRNAs) appear to have the most diverse mechanisms of action including competitive binding to miRNA targets, direct binding to mRNA, interactions with transcription factors, and facilitation of epigenetic modifications. Moreover, many lncRNAs display tissue-specific expression patterns suggesting an important regulatory role in organogenesis, yet the molecular mechanisms through which these molecules regulate cardiac and skeletal muscle development remains surprisingly limited. Given the structural and metabolic similarities of cardiac and skeletal muscle, it is likely that several lncRNAs expressed in both of these tissues have conserved functions in establishing the striated muscle phenotype. As many aspects of regeneration recapitulate development, understanding the role lncRNAs play in these processes may provide novel insights to improve regenerative therapeutic interventions in cardiac and skeletal muscle diseases. This review highlights key lncRNAs that function as regulators of development, regeneration, and disease in cardiac and skeletal muscle. Finally, we highlight lncRNAs encoded by imprinted genes in striated muscle and the contributions of these loci on the regulation of gene expression.

Sign in / Sign up

Export Citation Format

Share Document