Lactoferrin levels in cervical fluid from in vitro fertilization (IVF) patients. Correlation with IVF parameters.

2020 ◽  
Author(s):  
Estefania Massa ◽  
Fabián Pelusa ◽  
Agustina Lo Celso ◽  
María José Madariaga ◽  
Luciana Filocco ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Protein Concentration ◽  
Total Protein Concentration ◽  
Total Proteins ◽  
Reproductive Process ◽  
Assisted Reproduction Techniques ◽  
Vitro Fertilization ◽  
The Mean ◽  
Mean Concentration

Since our previous results suggest that LF might have roles in the reproductive process and its levels might change in the female tract as a response to various factors, the aim of this investigation was to assess whether LF levels in cervical secretions correlate with reproductive parameters from in vitro fertilization (IVF) patients. Cervical fluid samples were obtained from 34 women under 40 years old enrolled for assisted reproduction techniques and LF concentration was measured. The mean total protein concentration in all cervical fluid samples was 842.8±116.9 µg/ml. The mean concentration of LF was 0.73±0.06 ng LF/µg of total proteins. We observed that higher LF levels in cervical fluid correlated with lower IVF rates when all patients were analyzed; and this negative correlation was also sustained when only patients ≥35 years were studied. The mean LF concentration in cervical fluid was significantly lower among patients with normal IVF rates than in those with values ≤50%. Using a LF cutoff value of 0.83 ng LF/μg total proteins, the study revealed a significant association between the LF levels <0.83 ng LF/µg total proteins and IVF rates >50%. LF levels in cervical mucus could potentially be used as a marker of fertilization outcome.

scholarly journals IN VITRO FERTILITY TEST OF HUMAN SPERMATOZOA MEMBRANE PROTEIN FERTILIN BETA ANTIBODY IN MICE (Mus musculus Balb/c) AS IMMUNOCONTRACEPTIVE CANDIDATE

2017 ◽  
Vol 52 (3) ◽  
pp. 209
Author(s):  
Reny I’tishom ◽  
Doddy M Soebadi ◽  
Aucky Hinting ◽  
Hamdani Lunardhi ◽  
Rina Yudiwati
Keyword(s):  
In Vitro Fertilization ◽  
Membrane Protein ◽  
Mus Musculus ◽  
Human Spermatozoa ◽  
Control Group ◽  
Reproductive Process ◽  
Vitro Fertilization ◽  
Antibody Induction

One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.

scholarly journals Reproductive aspects of the male jaguar (Panthera onca): A review

2021 ◽  
Author(s):  
E. López-Pérez ◽  
F. Cortés-Villavicencio ◽  
C. Muñoz-García ◽  
J. Gallegos-Sánchez ◽  
Alejandro Ávalos-Rodríguez
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproduction ◽  
Reproductive System ◽  
Artificial Insemination ◽  
Relevant Information ◽  
Panthera Onca ◽  
Leopardus Pardalis ◽  
Assisted Reproduction Techniques ◽  
Vitro Fertilization

Objective: To describe the anatomy, morphology and physiology of the reproductive system of male jaguars, as well as assisted reproduction techniques. Methodology: A literature review on the anatomy and morphology of the jaguar´s reproductive system, its physiological characteristics and assisted reproduction techniques were carried out to document relevant information on the topic. Results: With this review, basic aspects of the morphology of the reproductive system of the jaguars are disclosed, although scarce knowledge is available on their reproduction. The advances in the collection, evaluation and cryopreservation of semen of this feline are shown, in addition to assisted reproduction techniques such as artificial insemination and in vitro fertilization, which have a great potential to safeguard the species. Study limitations: The jaguar, an emblematic species of Latinamerica, is an endangered species, like other wild felids species as ocelot (Leopardus pardalis) and margay (Leopardus wiedii), which makes it necessary to have a national assisted reproduction program. However, for this to be possible, information about their reproductive physiology is necessary, which is complicated in wild animals and even more so because the reproductive mechanisms greatly differ between felids species. There is scarce information in this regard from its free-living or Mexican zoos, it is for this reason necessary to generate such information. Conclusions: It is necessary to continue working on designing protocols for artificial insemination and other assisted reproduction techniques such as in-vitro fertilization specifically for male Panthera onca.

scholarly journals PP-25 EVOLUTION IN METHOD OF IN VITRO FERTILIZATION (CLASSICAL IVF OR ICSI) IN ASSISTED REPRODUCTION TECHNIQUES IN SPAIN

2012 ◽  
Vol 24 ◽  
pp. S14
Author(s):  
Yolanda Cabello ◽  
Esther Vidal ◽  
Fernando Prados ◽  
María José De Los Santos ◽  
Francisca Luceño ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproduction ◽  
Assisted Reproduction Techniques ◽  
Vitro Fertilization

scholarly journals Selection of male goat embryos in vitro by using swim-up of epididymal spermatozoa

2018 ◽  
Vol 42 (1) ◽  
pp. 79-86
Author(s):  
Ihsan H. S. Al-Timimi
Keyword(s):  
Polymerase Chain Reaction ◽  
In Vitro Fertilization ◽  
In Vitro Maturation ◽  
Chain Reaction ◽  
Vitro Fertilization ◽  
The Mean ◽  
Polymerase Chain ◽  
Epididymal Spermatozoa ◽  
Selection Of

     The main objectives of this study is the separation of X from Y bearing epididymal spermatozoa of local buck by swim-up, and the use of this spermatozoa for in vitro fertilization to determine the percentage of produced male and female embryos. The sex of produced embryo was identified by polymerase chain reaction. Testis of the local buck were obtained from Al-Shu'alah abattoir and the epididymal spermatozoa were harvested from the cauda by and submitted to in vitro maturation prior to separation of X from Y bearing spermatozoa and prior to their use for in vitro fertilization. For the separation of epididymal spermatozoa, swim-up technique was used with centrifugation at 200×g or 300×g. The centrifugation at 200×g showed that 41.84±1.39 % of spermatozoa were detected in the supernatant while the precipitate contained 50.69±0.71 and the mean of the sperm lost was 7.65±0.93. After centrifugation, spermatozoa in the supernatant were used for in vitro fertilization of matured oocytes. The sex of in vitro produced goat embryos was determined by polymerase chain reaction using specific primers to detect of SRY gene. The percentage of total goat embryos obtained after in vitro fertilization by sperms selected using swim-up at centrifugation force of 200×g recorded 79.66 % male embryos while female embryos recorded only 20.33 %. At the end, the results showed the ability of selection male embryos in caprine by application of swim-up technique on epididymal spermatozoa with centrifugation at 200×g.

scholarly journals The Role of MEMS in In-Vitro-Fertilization

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
I. D. Wijegunawardana ◽  
Y. W. R. Amarasinghe
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproduction ◽  
Success Rates ◽  
Assisted Reproduction Techniques ◽  
Vitro Fertilization ◽  
Viable Solution ◽  
Micro Electromechanical System ◽  
Experienced Individual

The assisted reproduction has been considered a viable solution for the infertility of humankind for more than four decades. In-Vitro-Fertilization (IVF) is one of the most successful assisted reproduction techniques, where the reproductive cell of the female partner is fertilized outside of her body. Initially, the IVF process has been conducted manually by an experienced embryologist. However, even with a highly experienced individual, the operation had extremely lower success rates due to the limited control in environmental conditions and the requirement of precise movements. Therefore, to address this technological deficit, the feasibility of the mechatronics devices for IVF procedures has been investigated. Among the different mechatronics concepts, micro-electromechanical system (MEMS) technologies have been gradually attracted to the IVF process and improved its capabilities. The purpose of this paper is to present a brief overview of the role of MEMS technologies in IVF. The article classifies the MEMS technologies in IVF based on their application in order to emphasize its contribution. In addition, the article extensively discusses the state-of-the-art mechatronic techniques utilized in Intracytoplasmic Sperm Injection (ICSI), one of the most popular techniques used in IVF. This review article expects to become extremely beneficial for the engineering researchers new to this field who seek critical information on IVF in simple terms with highlights on the possible advancements and challenges that may emerge in the future.

Surrogacy: The Parents' Story

2002 ◽  
Vol 91 (1) ◽  
pp. 201-219 ◽  
Author(s):  
Christine B. Kleinpeter
Keyword(s):  
Decision Making ◽  
In Vitro Fertilization ◽  
Qualitative Study ◽  
Conceptual Model ◽  
Helping Professionals ◽  
Vitro Fertilization ◽  
Telephone Interviews ◽  
The Mean

This qualitative study explored the experiences of 26 parents who were involved in surrogate parenting arrangements in a California-based surrogacy program. Participants were mostly white ( n = 23), married ( n = 25), females ( n = 24), with high levels of education and income. The mean age at the time of the first child's birth was 39 yr. ( SD = 5.06). The majority of parents reported having one ( n = 10) or two ( n = 8) children. All subjects reported infertility as their reason to explore surrogacy as a method of building a family. 18 participants chose in vitro fertilization as their method of conception. Telephone interviews explored their decision-making, method of fertilization, their relationship with their surrogate, and the support that they received during the surrogacy process. Results indicate that parents were able to anticipate some potential pitfalls prior to their experience but did not realize the importance of other potential difficulties. A conceptual model is presented with implications for helping professionals.

Prevalence of Common Thrombophilia and Antiphospholipid Antibodies in Unexplained Infertility Women Undergoing in Vitro Fertilization (IVF)

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 628-628
Author(s):  
Arie Steinvil ◽  
Raanan Raz ◽  
Shlomo A. Berliner ◽  
David M Steinberg ◽  
David Zeltser ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Success Rate ◽  
Conflicts Of Interest ◽  
Factor V Leiden ◽  
Unexplained Infertility ◽  
Factor V ◽  
Vitro Fertilization ◽  
History Of ◽  
The Mean

Abstract Abstract 628 Assisted reproductive technology (ART) is extensively used as a tool for pregnancy achievement in subfertile couples. Congenital and acquired thrombophilia have been suggested by some investigators to play a role in abnormal embryos implantation and placentation. The objective of this study was to assess the role of common thrombophilia in women with unexplained infertility undergoing in vitro fertilization (IVF). We enrolled five hundred ninety-four women from a large healthcare maintenance organization going through IVF and who had a thrombophilia workup, and compared them for prevalence of thrombophilia to two reference groups consisting of 637 fertile women from previous work and 17,337 women members of the same healthcare organization with no history of venous thromboembolisms. The mean age of the women at the first cycle of IVF was 30.9 years (SD: ±4.1).The mean number of IVF cycles was 7.3 (SD: 5.0), and the mean fertility success rate per woman was 14.6% (SD: 19.0). None of the common thrombophilia tested was found to be significantly associated with the number of IVF cycles or with lower fertility success rate. Rather, women who had APCR and/or factor V Leiden and lupus anticoagulant had significantly higher live birth rates (12.25% and 12.64%, respectively) in comparison to women who were tested negative (8.98% and 9.7%, respectively). Thus, hypercoagulability is not associated with failure to achieve pregnancy. These data suggest that neither screening for thrombophilia nor anticoagulant treatment is indicated in cases with unexplained reproductive failure. Disclosures: No relevant conflicts of interest to declare.

Guidelines for the Prevention of Multiple Pregnancy in Treatment by in Vitro Fertilization

1990 ◽  
Vol 39 (3) ◽  
pp. 371-378 ◽  
Author(s):  
F. Leroy ◽  
F. Puissant ◽  
P. Barlow ◽  
G. de Maertelaer
Keyword(s):  
In Vitro Fertilization ◽  
Multiple Pregnancy ◽  
Average Score ◽  
Multiple Gestation ◽  
Reliable Criterion ◽  
Vitro Fertilization ◽  
Stimulation Parameters ◽  
The Mean ◽  
High Age

AbstractWithin the same in vitro fertilization (IVF) program, treatment trials leading to single and multiple ongoing gestation were compared. Rates of cesarean delivery, prematurity and perinatal mortality were found much higher among twin and multiple IVF pregnancies. Our work thus attempts at defining characteristics of proneness to multiple gestation in IVF treatment, in order to try and avoid its occurrence. The mean vitality score of embryos replaced is the most reliable criterion for this purpose, enabling one to replace no more than two embryos when the average score is high. Age of the patient and cause of infertility are almost nondiscriminant in this respect. Ovarian stimulation parameters such as total dosage of gonadotropin treatment and level of estrogenic response, as well as numbers of oocytes and embryos obtained, may serve as secondary criteria for assessing the twinning risk.

scholarly journals Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

2019 ◽  
Vol 56 (3) ◽  
pp. e156894 ◽  
Author(s):  
Mayara Ellen Bardi de Moraes ◽  
Paulo Roberto Adona ◽  
Samuel Guemra ◽  
Tiago Henrique Camara De Bem ◽  
Moysés Dos Santos Miranda
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Follicle Stimulating Hormone ◽  
Vitro Fertilization ◽  
Average Recovery ◽  
Follicular Aspiration ◽  
Oocyte Recovery ◽  
The Mean ◽  
Stimulating Hormone

The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage.

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