The inorganic carbon-concentrating mechanism in cyanobacteria: induction and ecological significance

1998 ◽  
Vol 76 (6) ◽  
pp. 917-924 ◽  
Author(s):  
Aaron Kaplan ◽  
Michal Ronen-Tarazi ◽  
Hagit Zer ◽  
Rakefet Schwarz ◽  
Dan Tchernov ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
Ecological Significance ◽  
Carbon Concentrating Mechanism ◽  
Concentrating Mechanism ◽  
Open Questions

In this minireview we focus on certain aspects of the induction, function, and ecophysiological significance of the inorganic carbon-concentrating mechanism in cyanobacteria. Since this entire issue is dedicated to various aspects of this mechanism, we mainly discuss some of the recent studies in our laboratory and point to open questions and perspectives.Key words: CO2, cyanobacteria, inorganic carbon-concentrating mechanism, photosynthesis.

The inorganic carbon-concentrating mechanism in cyanobacteria: induction and ecological significance

1998 ◽  
Vol 76 (6) ◽  
pp. 917-924 ◽  
Author(s):  
Aaron Kaplan ◽  
Michal Ronen-Tarazi ◽  
Hagit Zer ◽  
Rakefet Schwarz ◽  
Dan Tchernov ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
Ecological Significance ◽  
Carbon Concentrating Mechanism ◽  
Concentrating Mechanism

Modulation of photosynthesis and inorganic carbon acquisition in a marine microalga by nitrogen, iron, and light availability

2005 ◽  
Vol 83 (7) ◽  
pp. 917-928 ◽  
Author(s):  
Erica B Young ◽  
John Beardall
Keyword(s):  
Inorganic Carbon ◽  
Dunaliella Tertiolecta ◽  
Initial Slope ◽  
Carbon Concentrating Mechanism ◽  
High Affinity ◽  
N Limitation ◽  
Marine Microalga ◽  
Concentrating Mechanism ◽  
Energy Limitation ◽  
Use Efficiency

The marine microalga Dunaliella tertiolecta Butcher expresses a high affinity for dissolved inorganic carbon (DIC) through a carbon-concentrating mechanism (CCM), known to be influenced by CO2 availability and instantaneous light supply. However, the regulation by light and nutrient supply during growth is less understood, although N and Fe limitation impose an energy limitation by compromising the photosynthetic apparatus. Dunaliella tertiolecta was grown under steady-state conditions of limited light, N, and Fe availability, and the affinity for DIC was measured under saturating light. High affinity DIC uptake capacity was maintained by D. tertiolecta under all growth-limiting conditions, but was modulated in response to the limiting resource. Affinity of photosynthesis for DIC(k0.5) was significantly reduced in cells grown under low light, both in turbidostats and in batch culture (p ≤ 0.03), although cell-normalized Pmax was not significantly affected. In contrast, N and Fe limitation resulted in a significant reduction in cell chlorophyll, Pmax, and maximum photosystem II quantum yield (Fv/Fm), but the affinity for DIC was enhanced with increasing N or Fe stress. While the affinity for DIC improved with increasing N stress (k0.5 < 17.8 µM at µ = 0.27 d–1 versus k0.5 > 26 µM at µ ≥ 0.77 d–1), light use efficiency (α) was impaired under N limitation, suggesting a trade-off between light harvesting capacity and active DIC uptake. Stable C isotope analysis of Fe-limited cells confirmed a lower fractionation by the most Fe-limited cells, consistent with the k0.5 data and more active DIC acquisition (δ13C = –19.56 at µ = 0.27 d–1 cf. δ13C = –26.28 at µ = 0.77 d–1). Assessment of affinity for DIC using k0.5 was supported by the close fit of P versus DIC curves to Michaelis–Menten kinetics; with the high DIC affinity of D. tertiolecta, there was poor resolution in the initial slope of the P versus DIC curve as a parameter of affinity for DIC. Enhanced DIC uptake efficiency under Fe and N limitation may relate to improved resource-use efficiency conferred by CCM activity.Key words: algae, carbon-concentrating mechanism, iron, light, nitrogen, nutrient limitation, photosynthesis.

scholarly journals Identification of Two Genes, sll0804 and slr1306, as Putative Components of the CO2-Concentrating Mechanism in the Cyanobacterium Synechocystis sp. Strain PCC 6803

2008 ◽  
Vol 190 (24) ◽  
pp. 8234-8237 ◽  
Author(s):  
Shulu Zhang ◽  
Kevin W. Spann ◽  
Laurie K. Frankel ◽  
James V. Moroney ◽  
Terry M. Bricker
Keyword(s):  
Inorganic Carbon ◽  
Carbon Concentrating Mechanism ◽  
Co2 Concentrating Mechanism ◽  
Concentrating Mechanism ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACT Insertional transposon mutations in the sll0804 and slr1306 genes were found to lead to a loss of optimal photoautotrophy in the cyanobacterium Synechocystis sp. strain PCC 6803 grown under ambient CO2 concentrations (350 ppm). Mutants containing these insertions (4BA2 and 3ZA12, respectively) could grow photoheterotrophically on glucose or photoautotrophically at elevated CO2 concentrations (50,000 ppm). Both of these mutants exhibited an impaired affinity for inorganic carbon. Consequently, the Sll0804 and Slr1306 proteins appear to be putative components of the carbon-concentrating mechanism in Synechocystis sp. strain PCC 6803.

scholarly journals Elevated Inorganic Carbon Concentrating Mechanism Confers Tolerance to High Light in an Arctic Chlorella sp. ArM0029B

2018 ◽  
Vol 9 ◽  
Author(s):  
Kwon Hwangbo ◽  
Jong-Min Lim ◽  
Seok-Won Jeong ◽  
Jayaraman Vikramathithan ◽  
Youn-Il Park ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
High Light ◽  
Carbon Concentrating Mechanism ◽  
Chlorella Sp ◽  
Concentrating Mechanism

The Inorganic Carbon-Concentrating Mechanism of Cyanobacteria

1999 ◽  
pp. 561-571
Author(s):  
Aaron Kaplan ◽  
Michal Ronen-Tarazi ◽  
Dan Tchernov ◽  
David J. Bonfil ◽  
Hagit Zer ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
Carbon Concentrating Mechanism ◽  
Concentrating Mechanism

scholarly journals Acclimation to low [CO2] by an inorganic carbon-concentrating mechanism in Cyanophora paradoxa

2007 ◽  
Vol 30 (11) ◽  
pp. 1422-1435 ◽  
Author(s):  
S. C. BUREY ◽  
V. POROYKO ◽  
Z. N. ERGEN ◽  
S. FATHI-NEJAD ◽  
C. SCHÜLLER ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
Cyanophora Paradoxa ◽  
Carbon Concentrating Mechanism ◽  
Low Co2 ◽  
Concentrating Mechanism

Influence of the carbon concentrating mechanism on carbon stable isotope discrimination by the marine diatom Thalassiosira pseudonana

1998 ◽  
Vol 76 (6) ◽  
pp. 1098-1103 ◽  
Author(s):  
Anthony S Fielding ◽  
David H Turpin ◽  
Robert D Guy ◽  
Stephen E Calvert ◽  
David W Crawford ◽  
...  
Keyword(s):  
Carbon Isotope ◽  
Inorganic Carbon ◽  
Carbon Isotope Discrimination ◽  
Thalassiosira Pseudonana ◽  
Marine Phytoplankton ◽  
Marine Diatom ◽  
Carbon Concentrating Mechanism ◽  
Fractionation Factor ◽  
Isotope Discrimination ◽  
Concentrating Mechanism

There is no clear explanation why phytoplankton δ13C values are more negative in colder waters, but one current theory suggests that because colder waters hold more CO2, there is less diffusional limitation of CO2. This results in more discrimination against 13C and more negative phytoplankton δ13Cvalues. However, many species are able to actively take up CO2 or HCO3-, the latter being the major inorganic carbon species present in the dissolved inorganic carbon (DIC) pool of modern oceans. A previous study suggests that carbon concentrating mechanism (CCM) induction would affect carbon isotope discrimination, and this study confirms the presence of a relationship between discrimination and induction of a CCM in the marine diatom Thalassiosira pseudonana. CCM induction was measured by determining the half-saturation constant of photosynthesis (K0.5DIC). Values of K0.5DIC increased from 85 to 470 m M DIC over a range of ambient DIC levels from 0.2 to 2.7 mM. The fractionation factor increased from 10 to 21.3omicron over this same range. There was a significant relationship between K0.5DIC and the fractionation factor suggesting that CCM induction state influences carbon isotope discrimination. Other factors that influence discrimination may act through CCM induction.Key words: carbon isotope discrimination, carbon concentrating mechanism, Thalassiosira pseudonana, active carbon uptake, marine phytoplankton.

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