Seasonal and needle age-dependent changes of the endophytic mycobiota in Pinus thunbergii and Pinus densiflora needles

1998 ◽  
Vol 76 (2) ◽  
pp. 245-250 ◽  
Author(s):  
Kunihiko Hata ◽  
Kazuyoshi Futai ◽  
Mitsuya Tsuda
Keyword(s):  
Tissue Culture ◽  
Endophytic Fungi ◽  
Pinus Densiflora ◽  
Pinus Thunbergii ◽  
Pine Needle ◽  
Needle Age ◽  
Detection Frequency ◽  
Age Dependent ◽  
Monthly Changes ◽  
Pine Species

Monthly changes in the endophytic mycobiota in Pinus thunbergii Parl. and Pinus densiflora Sieb. et Zucc. needles were detected by tissue culture. Endophytic mycobiota of these pine needles were dominated by four taxa: the Leptostroma anamorph of the Lophodermium pinastri (Schrad. ex Hook.) Chev. complex, Phialocephala sp., Cenangium ferruginosum Fr.:Fr., and an unidentified hyphomycete species (BrS). The mycobiota differed with host pine species, position on the needle, and needle age. Virtually no endophytes were detected in needles just after emergence, but most segments of the older needles harbored endophytes. The endophytic mycobiota changed slowly with needle aging, except for the periods of rapid increase in the detection frequency of Leptostroma and Phialocephala sp. Detection frequency of Leptostroma, the most frequently detected endophyte taxon in the needles of the two pine species, continuously increased with needle aging, while that of Phialocephala sp., the endophyte mainly detected from the basal segments of Pinus densiflora, slowly decreased with needle aging after a massive emergence in current-year needles.Key words: Pinus thunbergii, Pinus densiflora, pine needle, endophytic fungi.

Endophytic fungi associated with healthy pine needles and needles infested by the pine needle gall midge, Thecodiplosis japonensis

1995 ◽  
Vol 73 (3) ◽  
pp. 384-390 ◽  
Author(s):  
Kunihiko Hata ◽  
Kazuyoshi Futai
Keyword(s):  
Endophytic Fungi ◽  
Pinus Densiflora ◽  
Gall Midge ◽  
Pine Needles ◽  
Basal Region ◽  
Ecological Groups ◽  
Gall Formation ◽  
Pine Needle ◽  
Hybrid Pine ◽  
Pine Needle Gall Midge

The endophytic mycobiota of the galls of pine needle gall midge, Thecodiplosis japonensis, and healthy current-year needles of Pinus densiflora and the F2 hybrid pine (Pinus thunbergii × (P. thunbergii × P. densiflora)) was compared. Phialocephala sp. was the dominant endophyte isolated from the galls and from the basal regions of healthy needles. Species richness in endophytes was facilitated in galls compared to the basal region of healthy needles, and the commencement of their colonization was hastened in galls. In the early stages of gall formation, however, no endophyte was isolated, suggesting that gall endophytes are not carried into the galls by the midge. Endophytes of healthy and galled pine needles were divided into two groups: position-specific fungi, which showed intrinsically restricted distributions on needles; and gall-specific fungi. These two groups might represent different ecological groups of endophytes. Possible interactions between endophytes and the pine needle gall midge are also discussed. Key words: endophytic fungi, Pinus densiflora, F2 hybrid pine, pine needle gall midge.

Immunomodulatory Activity of Pine Needle (Pinus densiflora) Extracts in Macrophages

2006 ◽  
Vol 11 (2) ◽  
pp. 105-109 ◽  
Author(s):  
Hye-Sook Choi ◽  
Do Hang ◽  
Seong-Jun Cho ◽  
Se-Chan Kang ◽  
Eun-Soo Sohn ◽  
...  
Keyword(s):  
Pinus Densiflora ◽  
Immunomodulatory Activity ◽  
Pine Needle

Isolation of fungal endophytes from black spruce (Picea mariana) dormant buds and needles from New Brunswick, Canada

1992 ◽  
Vol 70 (9) ◽  
pp. 1754-1757 ◽  
Author(s):  
J. A. Johnson ◽  
N. J. Whitney
Keyword(s):  
New Brunswick ◽  
Key Words ◽  
Endophytic Fungi ◽  
Picea Mariana ◽  
Black Spruce ◽  
Fungal Endophytes ◽  
The Other ◽  
Age Classes ◽  
Needle Age

Endophytic fungi were isolated from black spruce (Picea mariana (Mill.) BSP) dormant buds and needles of four age-classes. Only one isolate was obtained from 400 buds. A total of 914 isolates were obtained from the needles. With increasing needle age the rate of colonization increased between current-year and 3-year-old needles from 4 to 90%, respectively. The needle segment attached to the twig was colonized more often (p < 0.05) than other segments overall and for 6 of the 11 taxa isolated. The first endophyte from current-year needles was isolated on July 14, 1988, but endophytes were obtained from needles of the other age-classes (1, 2, and 3 years) on all sampling dates between June 10 and September 16, 1988. Key words: endophyte, Picea mariana, dormand buds, needles.

Sign in / Sign up

Export Citation Format

Share Document