Variation in mating competence and random amplified polymorphic DNA inLaccaria bicolor(Agaricales) associated with three tree host species

1995 ◽  
Vol 73 (6) ◽  
pp. 884-890 ◽  
Author(s):  
V. L. Raffle ◽  
N. A. Anderson ◽  
G. R. Furnier ◽  
R. L. Doudrick
Keyword(s):  
Populus Tremuloides ◽  
Host Species ◽  
Pinus Banksiana ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Pinus Resinosa ◽  
Ectomycorrhizal Fungus ◽  
Age Classes ◽  
Single Spore ◽  
Incompatibility Group

We examined the population genetic structure of the ectomycorrhizal fungus Laccaria bicolor (Maire) Orton using single spore homokaryotic cultures from 33 basidiomes collected in northern Minnesota in association with red pine (Pinus resinosa Ait.), jack pine (Pinus banksiana Lamb.), and trembling aspen (Populus tremuloides Michx.) of three age-classes (0–20 years, 21–40 years, and > 41 years). Mating competence between cultures of isolates, as determined by the presence of clamp connections, revealed the presence of two subpopulations that were not freely interbreeding, one composed of 29 dikaryons, the other of 3 dikaryons. Phenetic cluster analysis using random amplified polymorphic DNA markers did not reveal differentiation between these subpopulations. Clustering failed to reveal genetically distinct groups based on incompatibility group, tree host species, or geographic origin of isolates. Key words: heterogenic incompatibility, RAPD, population genetics.

Distribution of Armillaria ostoyae genets in a Pinus resinosa – Pinus banksiana forest

1995 ◽  
Vol 73 (5) ◽  
pp. 776-787 ◽  
Author(s):  
David M. Rizzo ◽  
Robert A. Blanchette ◽  
Georgiana May
Keyword(s):  
Pinus Banksiana ◽  
Pinus Resinosa ◽  
Molecular Data ◽  
Incompatibility Group ◽  
Ecological Processes ◽  
Somatic Incompatibility ◽  
Mitochondrial Markers ◽  
Armillaria Ostoyae ◽  
Mitochondrial Haplotypes ◽  
Single Exception

Diploid isolates of Armillaria ostoyae were obtained from 278 trees (including stumps, Pinus banksiana seedlings, and hardwood regeneration) and 78 solitary basidiomes on three 50 × 25 m plots (two clearcut and one uncut) in a Pinus resinosa – P. banksiana stand in northern Minnesota. Based on pairings among 439 isolates, 16 somatic incompatibility groups of A. ostoyae were distinguished on the three plots. Analysis of nuclear and mitochondrial markers suggests that many of the A. ostoyae genets are closely related and were possibly established by sib-related basidiospores. Analysis of molecular markers of 95 isolates representing the 16 somatic incompatibility groups delineated 16 nuclear and 8 mitochondrial haplotypes. All isolates tested within a somatic incompatibility group were identical for nuclear and mitochondrial haplotypes; a single exception consisted of a somatic incompatibility group with three different nuclear genotypes. The combination of somatic incompatibility and molecular data indicates the presence of at least 18 genets on the site. Large (up to 140-m diameter), spatially continuous genets intermingled with small, recently established genets and older, fragmented genets. The approach identified genetic and ecological processes; these include breeding strategies and pathogenicity. Key words: Armillaria ostoyae, somatic incompatibility, population structure, mitochondria.

scholarly journals Genetic Variability of Canadian Populations of the Sapstain Fungus Ophiostoma piceae

2001 ◽  
Vol 91 (4) ◽  
pp. 369-376 ◽  
Author(s):  
Pierre Gagné ◽  
Dian-Qing Yang ◽  
Richard C. Hamelin ◽  
Louis Bernier
Keyword(s):  
Genetic Variability ◽  
Genetic Differentiation ◽  
Picea Glauca ◽  
Pinus Banksiana ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Abies Balsamea ◽  
Principal Component ◽  
Single Spore ◽  
Ophiostoma Piceae

Genetic diversity was studied in seven Canadian populations of Ophiostoma piceae, the most prevalent sapstain fungus in Canadian softwoods. A total of 239 single-spore isolates were recovered following a systematic survey of sapstain fungi in logs and lumber at seven selected sawmills in six Canadian provinces (British Columbia, Alberta, Saskatchewan, Ontario, Québec, and New Brunswick). Sampling was carried out on five commercially important softwood species: balsam fir (Abies balsamea), white spruce (Picea glauca), black spruce (Picea mariana), jack pine (Pinus banksiana), and lodgepole pine (Pinus con-torta var. latifolia). The A and B mating types occurred at equal frequency (MAT A/ MAT B = 1.00:1.13) over all populations. Pseudo-allelic frequencies were estimated at each of 24 putative genetic loci by scoring for presence or absence of random amplified polymorphic DNA fragments generated by five primers. A total of 237 haplotypes were found among the 239 isolates, revealing a high level of genotypic diversity among isolates. Total gene diversity (HT = 0.414) was mostly attributable to diversity within populations (HS = 0.369). Thus, only 11.2% of the total variability was attributable to frequency differences among populations. An analysis of molecular variance revealed that most genetic variability occurred within subpopulations within mills (84.3%; P < 0.001), whereas low but statistically significant levels of genetic differentiation were also observed among subpopulations within populations (5.4%; P < 0.001) and among populations (10.3%; P < 0.001). Estimates of Nei' genetic distances were not correlated with geographic distances among sampling locations (r = -0.092; P = 0.310), although principal component analysis indicated that subpopulations located east of Saskatchewan were grouped on the same side of the second principal component axis. Overall, results suggest moderate genetic differentiation of O. piceae in Canada, which is consistent with the observation that sexual reproduction is frequently observed in this fungus.

NOTES ON THE CANADIAN TUCKAHOE, ITS OCCURRENCE IN CANADA AND THE INTERFERTILITY OF ITS PERFECT STAGE, POLYPORUS TUBERASTER JACQ. EX FRIES

1951 ◽  
Vol 29 (2) ◽  
pp. 147-157 ◽  
Author(s):  
T. C. Vanterpool ◽  
Ruth Macrae
Keyword(s):  
Populus Tremuloides ◽  
Western Canada ◽  
Single Spore ◽  
Canadian Prairies ◽  
Perfect Stage

The Canadian tuckahoe is the perennial sclerotium of Polyporus tuberaster jacq. ex Fries. It is commonly found in the parkland belt of the Canadian prairies where land supporting, virgin poplar groves, mainly Populus tremuloides Michx., is being brought under cultivation. Sporophore as many as three to a single sclerotium, appear in late June and July. Interfertility studies with single spore cultures isolated from sporophores derived from four sources in Western Canada and from one source in Italy have shown that both the Canadian fungus and the European P. tuberaster are heterothallic, have the tetrapolar type of interfertility, and are interfertile.

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H I Amadou ◽  
P J Bebeli ◽  
P J Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Bambara Groundnut ◽  
International Institute ◽  
Tropical Agriculture ◽  
Vigna Subterranea ◽  
Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

scholarly journals Limited Genetic Diversity in North American Isolates of Phytophthora erythroseptica Pathogenic to Potato Based on RAPD Analysis

Plant Disease ◽  
2005 ◽  
Vol 89 (4) ◽  
pp. 380-384 ◽  
Author(s):  
Rick D. Peters ◽  
Rod J. Clark ◽  
Albert D. Coffin ◽  
Antony V. Sturz ◽  
David H. Lambert ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
New Brunswick ◽  
Prince Edward Island ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
The United States ◽  
Vitro Assay ◽  
Phytophthora Erythroseptica

Pink rot of potato (Solanum tuberosum), caused by Phytophthora erythroseptica, is found wherever potatoes are grown, and in the last decade, it has reemerged as an economically important disease in Canada and the United States. A selection of isolates of P. erythroseptica from major potato-growing regions in North America, namely Prince Edward Island and New Brunswick, Canada, and Maine and Idaho, U.S.A., was assessed for genetic diversity with randomly chosen decanucleotide primers which were used to amplify regions of DNA to reveal polymorphisms among templates (random amplified polymorphic DNA [RAPD]). The isolates varied in their geographic origin as well as in their sensitivity to mefenoxam, as determined by an in vitro assay. In three separate RAPD screens (I, II, and III) with 23 isolates of P. erythroseptica chosen from a larger collection, 1,410, 369, and 316 robust, scorable bands were amplified, respectively. However, among the bands amplified in screens I, II, and III, only 3, 1, and 3 bands, respectively, were polymorphic. When three primers yielding polymorphisms were used to screen 106 isolates from Prince Edward Island and New Brunswick, or a representative collection of 32 isolates from Prince Edward Island, New Brunswick, Maine, and Idaho, no major variation was discovered. RAPD markers were not correlated with geographic origin or mefenoxam sensitivity of the isolates. From an evolutionary standpoint, the absence of genetic diversity among the isolates of P. erythroseptica we examined may be attributable to the relatively recent introduction of a small founding population of the pathogen in North America.

scholarly journals Assessing the Translucence and Color-Change Methods for Estimating Sapwood Depth in Three Boreal Species

Forests ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 686 ◽  
Author(s):  
M. Quiñonez-Piñón ◽  
Caterina Valeo
Keyword(s):  
Populus Tremuloides ◽  
Picea Glauca ◽  
Pinus Banksiana ◽  
Wood Anatomy ◽  
Color Change ◽  
Microscopic Analysis ◽  
Forest Monitoring ◽  
Statistical Comparison ◽  
The Face ◽  
Species Specific

The translucence and color change in wood methods, which are commonly used to differentiate sapwood from heartwood in tree cores, are compared against the microscopic analysis of wood anatomy method for determining sapwood depth. The translucence method was tested on collected wood cores of White Spruce (Picea glauca (Moench) Voss) and Jack Pine (Pinus banksiana Lamb.). The color change in wood method was tested on Trembling Aspen (Populus tremuloides Michx.). For every statistical comparison, sapwood depth values obtained with the translucence or color-change methods were significantly different from those obtained using the microscopic analysis. Using the sapwood depth values obtained with microscopy as a reference, the bias associated with the translucence or color-change methods used on Picea glauca, Pinus banksiana and Populus tremuloides constantly under- or overestimated sapwood depths within −0.3 cm to 1.6 cm; −4.9 cm to 0.5 cm; and 0 to 1.8 cm, respectively. The different ranges of over- and underestimation arise from species-specific anatomical characteristics. Estimates for the errors in sapwood depth, when the depth is measured using either the translucence or color-change methods, are presented. These relationships and research outcomes will lead to more efficient forest monitoring and improved estimates of forest water balance, which in turn will lead to improved forest management in the face of climate change.

scholarly journals Taper Equations for Five Major Commercial Tree Species in Manitoba, Canada

2007 ◽  
Vol 22 (3) ◽  
pp. 163-170 ◽  
Author(s):  
Ryan J. Klos ◽  
G. Geoff Wang ◽  
Qing-Lai Dang ◽  
Ed W. East
Keyword(s):  
Populus Tremuloides ◽  
Picea Glauca ◽  
Pinus Banksiana ◽  
White Spruce ◽  
Jack Pine ◽  
Volume Estimation ◽  
Trembling Aspen ◽  
Stem Form ◽  
Taper Equation ◽  
Taper Equations

Abstract Kozak's variable exponent taper equation was fitted for balsam poplar (Populus balsamifera L.), trembling aspen (Populus tremuloides Michx.), white spruce (Picea glauca [Moench] Voss), black spruce (Picea mariana [Mill.] B.S.P.), and jack pine (Pinus banksiana Lamb.) in Manitoba. Stem taper variability between two ecozones (i.e., Boreal Shield and Boreal Plains) were tested using the F-test. Regional differences were observed for trembling aspen, white spruce, and jack pine, and for those species, separate ecozone-specific taper equations were developed. However, the gross total volume estimates using the ecozone-specific equations were different from those of the provincial equations by only 2 percent. Although the regional difference in stem form was marginal within a province, a difference of approximately 7 percent of gross total volume estimation was found when our provincial taper equations were compared with those developed in Alberta and Saskatchewan. These results suggest that stem form variation increases with spatial scale and that a single taper equation for each species may be sufficient for each province.

The effect of pedogenetic processes on the distribution of phosphorus, calcium and magnesium in Gray Luvisols

1991 ◽  
Vol 71 (4) ◽  
pp. 397-410 ◽  
Author(s):  
X. J. Xiao ◽  
D. W. Anderson ◽  
J. R. Bettany
Keyword(s):  
Populus Tremuloides ◽  
Picea Glauca ◽  
Pinus Banksiana ◽  
Black Spruce ◽  
Mineral Soil ◽  
Coniferous Forest ◽  
Soil Formation ◽  
Podzolic Soils ◽  
Exchangeable Ca ◽  
Phosphorus Losses

Pedogenesis and its effect on calcium (Ca), magnesium (Mg) and phosphorus (P) was studied on a sequence of seven Gray Luvisol soils in central Saskatchewan. The soils were formed on calcareous glacial till under trembling aspen (Populus tremuloides Michx), mixedwood (aspen and white spruce) (Picea glauca (Moench) Voss)) and coniferous (black spruce and jack pine) (Picea mariana (Mill) BSP and Pinus banksiana Lamb) forests. Soils under aspen had the highest concentration of total and exchangeable Ca and Mg in litter layers and Ae horizons, and had Ae and Bt horizons that were least acidic. The most acidic Ae and Bt horizons and lowest amounts of Ca and Mg occurred under coniferous forests, whereas the soils under mixedwood stands were intermediate. The thickness of eluvial (Ae and AB) horizons increased along the aspen to coniferous sequence. All soils had about 40% less P in their A and B horizons than was calculated to have been present at the start of soil formation. The greatest decrease in P was observed in the thickest and most acidic soil under coniferous forest. The present litter layers and vegetation make up only a small proportion of the P removed from the mineral soil. Unusually large amounts of P appear to have been translocated from A and B horizons during development of Gray Luvisols, in comparison to Chernozemic or even Podzolic soils. Our hypothesis proposes that P is ineffectively retained in the solum as P-clay-humus, or iron-P complexes and that organic P moves along with the soil water, laterally and downslope through permeable Ae horizon over less permeable Bt horizons, or vertically through macropores. Key words: Boreal forest, nutrient cycling, phosphorus losses, weathering, soil formation

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