Measurement of heat injury in plant tissue by using electrical impedance analysis

1993 ◽  
Vol 71 (12) ◽  
pp. 1605-1611 ◽  
Author(s):  
M. I. N. Zhang ◽  
J. H. M. Willison ◽  
M. A. Cox ◽  
S. A. Hall
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Electrical Impedance ◽  
Solanum Tuberosum L ◽  
Impedance Analysis ◽  
Membrane Capacitance ◽  
Temperature Changes ◽  
Heat Injury ◽  
Membrane Thermostability ◽  
Extracellular Resistance

Electrical impedance spectra (100 Hz to 800 kHz) were measured in pieces of potato (Solanum tuberosum L.) tuber tissue that had been heat stressed. Analysis of the impedance data based on equivalent circuits showed that tissue injury was revealed as decreases in membrane capacitance and extracellular resistance. By using a single piece of tissue, membrane thermostability (in relation to heat stress temperature or time of heat stress) was measured. Nonstressful temperature changes produced changes in tissue parameters that were fully reversible when the direction of temperature change was reversed. Stressful temperature changes produced irreversible changes in parameters. During heat injury, decrease in extracellular resistance always preceded a decrease in tonoplast capacitance. It is suggested that two stages may be involved in heat injury to membranes: functional injury leading to electrolyte leakage to extracellular space, and structural damage leading to membrane disintegration. It is concluded that electrical impedance analysis is useful in plant heat stress physiology. Key words: potato (Solanum tuberosum L.) tuber, electrical impedance, membrane capacitance, heat injury, membrane thermostability.

scholarly journals Seleksi Kultivar Kentang (Solanum tuberosum L.) Berdasarkan Penampilan Karakter Agronomis di Dataran Medium Kabupaten Garut

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Jajang Supriatna ◽  
Resti Fajarfika ◽  
Asep Bagja ◽  
Juniarti P Sahat
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Solanum Tuberosum L

Penanaman kentang pada kawasan dataran medium akan dihadapkan dengan masalah cekaman lingkungan terutama suhu tinggi (heat stress) yang menyebabkan adanya perubahan penampilan sebagai respons terhadap cekaman. Adanya perbedaan respons diantara kultivar menunjukkan tingkat adaptasi yang beragam. Sebuah penelitian yang bertujuan untuk menyeleksi kultivar kentang berdasarkan penampilan karakter agronomis di dataran medium Kabupaten Garut sebagai langkah awal seleksi dan evaluasi materi genetik potensial dalam pengembangan kultivar kentang adaptif dataran medium Kabupaten Garut. Percobaan telah dilaksanakan pada bulan Juli sampai Oktober 2018 di Tarogong Kaler Kabupaten Garut dengan ketinggian 732 meter di atas permukaan laut (m dpl). Percobaan disusun dalam Rancangan Acak Kelompok dengan menggunakan 15 kultivar kentang sebagai perlakuan dan diulang sebanyak tiga kali. Kultivar-kultivar yang digunakan terdiri dari 14 kultivar uji yaitu 1) Andina, 2) Sangkuriang, 3) Cipanas, 4) AR-08, 5) Amabile, 6) Atlantik Malang, 7) Dayang Sumbi, 8) GM-05, 9) Merbabu-17, 10) Vernei, 11) Tedzo MZ, 12) Median, 13) Erika, dan 14) Granola, serta 1 kultivar pembanding yaitu Olimpus. Hasil penelitian menunjukkan terseleksi sejumlah kultivar kentang yang memiliki penampilan agronomis lebih baik dibandingkan dengan kultivar pembanding diantaranya 2 kultivar berdasarkan tinggi tanaman, 2 kultivar berdasarkan luas daun, 5 kultivar berdasarkan jumlah umbi per tanaman dan 10 kultivar berdasarkan diameter umbi. Kultivar GM-05 dan Vernei memiliki kemampuan adaptasi yang baik pada kawasan dataran medium Kabupaten Garut berdasarkan karakter tinggi tanaman, luas daun dan jumlah umbi per tanaman. Kata Kunci: Solanum tuberosum L.; karakter agronomis; heat stress; dataran medium

Electrical impedance analysis in plant tissues: in vivo detection of freezing injury

1992 ◽  
Vol 70 (11) ◽  
pp. 2254-2258 ◽  
Author(s):  
M. I. N. Zhang ◽  
J. H. M. Willison
Keyword(s):  
Electrical Impedance ◽  
Solanum Tuberosum L ◽  
Nonlinear Least Squares ◽  
Impedance Analysis ◽  
Freezing Injury ◽  
Electrode Polarization ◽  
Electrode Impedance ◽  
Membrane Injury ◽  
Tissue Impedance

Freezing injury of potato tuber tissue was studied by measuring electrical impedance, in the range of 100 Hz to 800 KHz, while the tissue was subjected to a −3 °C environment. It was found that a greater proportion of total impedance was due to electrode polarization in frozen tissues than in nonfrozen tissues. In frozen tissue, electrode impedance could be so great that tissue impedance could not be measured reliably. Analysis of tissue impedance using complex nonlinear least squares revealed some dynamics of the process of tissue freezing. After 1 h of exposure to freezing conditions, extracellular resistance began a sustained decrease. This can be explained by electrolyte leakage to extracellular space, presumably as a result of membrane injury. The capacitances of both plasma membrane and tonoplast also decreased with freezing. Key words: potato (Solanum tuberosum L.) tuber, electrical impedance, freezing injury, membrane capacitance.

In-season heat stress compromises postharvest quality and low-temperature sweetening resistance in potato (Solanum tuberosum L.)

Planta ◽  
2014 ◽  
Vol 239 (6) ◽  
pp. 1243-1263 ◽  
Author(s):  
Daniel H. Zommick ◽  
Lisa O. Knowles ◽  
Mark J. Pavek ◽  
N. Richard Knowles
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Low Temperature ◽  
Solanum Tuberosum L ◽  
Postharvest Quality ◽  
Low Temperature Sweetening

Electrical impedance analysis in plant tissues: The effect of freeze-thaw injury on the electrical properties of potato tuber and carrot root tissues

1992 ◽  
Vol 72 (2) ◽  
pp. 545-553 ◽  
Author(s):  
M. I. N. Zhang ◽  
J. H. M. Willison
Keyword(s):  
Plasma Membrane ◽  
Potato Tuber ◽  
Electrical Impedance ◽  
Membrane Capacitance ◽  
Potato Tubers ◽  
Carrot Root ◽  
Freeze Thaw ◽  
Intracellular Resistance ◽  
Root Tissues ◽  
Extracellular Resistance

Electrical impedances in the range 100 Hz to 800 KHz were measured in potato tubers and carrot root cortex both before and after freeze-thaw cycles. These impedance data were analyzed using equivalent circuit modelling based on complex nonlinear least squares (CNLS) (Zhang and Willison 1991a). After freezing to and thawing from −3, −6, −9 and −12 °C, carrot root tissues were best characterized by a double shell model composed of extracellular resistance, cytoplasmic resistance, plasma membrance capacitance, vacuole interior resistance, and tonoplast capacitance. Although freeze–thaw cycles to −3 or −6 °C did not kill the carrot tissues, extracellular resistance and vacuole interior resistance were halved relative to control tissues, and cytoplasmic resistance decreased to a third of the control value. Plasma membrane capacitance and tonoplast capacitance were not affected by the −3 and −6 °C noninjurious stresses. After carrot root or potato tuber tissues had been killed by freezing (−3 °C for potato tuber and −18 °C for carrot), the tissues were best represented by a single-shell model composed of extravesicular resistance, intravesicular resistance, and vesicle membrane. Compared with unfrozen controls, freeze-killed potato tubers were characterized by a 30-fold decrease in extracellular resistance, a 7-fold decrease in intracellular resistance, and a 10-fold decrease in plasma membrane capacitance. Freeze-killed carrot roots were characterized by a 30-fold decrease in extracellular resistance, 3-fold decrease in intracellular resistance, and 3.5-fold decrease in plasma membrane capacitance. These results are compatible with massive rupturing of protoplasts during lethal freeze–thaw injury.Key words: Solanum tuberosum L., Daucus carota L., freeze–thaw injury, electrical impedance

scholarly journals Temperature changes the dynamics of trace element accumulation in Solanum tuberosum L.

2011 ◽  
Vol 112 (3-4) ◽  
pp. 655-672 ◽  
Author(s):  
Yu Li ◽  
Qiang Zhang ◽  
Runyuan Wang ◽  
Xin Gou ◽  
Heling Wang ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Trace Element ◽  
Solanum Tuberosum L ◽  
Temperature Changes ◽  
Element Accumulation ◽  
Trace Element Accumulation

scholarly journals Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)

2012 ◽  
Vol 64 (1) ◽  
pp. 135-144 ◽  
Author(s):  
Jelena Savic ◽  
Ivana Dragicevic ◽  
D. Pantelic ◽  
Jasmina Oljaca ◽  
Ivana Momcilovic
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Heat Shock ◽  
Heat Tolerance ◽  
Solanum Tuberosum L ◽  
Immunoblot Analysis ◽  
Control Treatment ◽  
Ex Vitro ◽  
Heat Tolerant

We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (?Laura?), moderately sensitive (?Liseta?) and heat-sensitive (?Agria?) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ?Laura? accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ?Liseta? and ?Agria?. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.

scholarly journals Introduction of Arabidopsis’s heat shock factor HsfA1d mitigates adverse effects of heat stress on potato (Solanum tuberosum L.) plant

2020 ◽  
Vol 25 (1) ◽  
pp. 57-63
Author(s):  
Zamarud Shah ◽  
Safdar Hussain Shah ◽  
Gul Shad Ali ◽  
Iqbal Munir ◽  
Raham Sher Khan ◽  
...  
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Heat Shock ◽  
Adverse Effects ◽  
Solanum Tuberosum L ◽  
Heat Shock Factor

scholarly journals Interactive Responses of Potato (Solanum tuberosum L.) Plants to Heat Stress and Infection With Potato Virus Y

2018 ◽  
Vol 9 ◽  
Author(s):  
Svetlana Makarova ◽  
Antonida Makhotenko ◽  
Nadezhda Spechenkova ◽  
Andrew J. Love ◽  
Natalia O. Kalinina ◽  
...  
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Solanum Tuberosum L

scholarly journals Influence of heat stress on growth and physiological activities of potato (Solanum tuberosum L.)

Phyton ◽  
2018 ◽  
Vol 87 (1) ◽  
pp. 225-230
Author(s):  
Naz N ◽  
F Durrani ◽  
Z Shah ◽  
NA Khan ◽  
I Ullah
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Solanum Tuberosum L
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