Isoenzyme analysis of teliospores from species of Anthracoidea parasitic on Carex species

1993 ◽  
Vol 71 (11) ◽  
pp. 1406-1413 ◽  
Author(s):  
Vanamo Salo ◽  
Robin Sen
Keyword(s):  
Genetic Variation ◽  
Banding Pattern ◽  
Gel Electrophoresis ◽  
Cell Walls ◽  
Morphological Character ◽  
Polyacrylamide Gel Electrophoresis ◽  
Isoenzyme Analysis ◽  
Banding Patterns ◽  
Multiple Loci ◽  
Smut Spores

The smut sori of five Anthracoidea species, A. echinospora, A. heterospora, A. buxbaumii, A. limosa, and A. paniceae, removed from infected inflorescences of 5 Carex host plant species collected from 17 natural Finnish populations, were surveyed by isozyme analysis. The protein extracts of individual sori, containing teliospores (smut spores), together with uninfected utricles, were subjected to polyacrylamide gel electrophoresis and stained for esterase and four peptidase enzymes. Single sori, representing individual infection units, were sufficient to reveal considerable esterase and peptidase isozyme activities, whereas little or no activity was seen in the control utricle extracts. High levels of genetic variation as expressed by the detection of multiple loci and allelism were recorded between species and various populations, but variation within populations was lower. Peptidase banding patterns, in particular, proved to be useful in differentiating species: A. heterospora and A. echinospora, parasitic on the same plant, were clearly distinguished by their isozyme patterns, and the three remaining smuts belonging to the subgenus Proceres could be separated from one another. One morphological character, presence of numerous thick internal swellings in the cell walls of teliospores, seemed to correlate well with banding pattern differences in A. heterospora. Key words: isozymes, electrophoresis, Anthracoidea, Carex, chemotaxonomy.

Immunoblot patterns of Giardia duodenalis isolates from different hosts and geographical locations

1990 ◽  
Vol 36 (1) ◽  
pp. 42-46 ◽  
Author(s):  
M. Forrest ◽  
J. Isaac-Renton ◽  
W. Bowie
Keyword(s):  
Key Words ◽  
Banding Pattern ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Giardia Duodenalis ◽  
Immunoblot Analysis ◽  
Polyacrylamide Gels ◽  
Banding Patterns ◽  
Antigenic Reactivity ◽  
Geographical Locations

Eighteen isolates of Giardia duodenalis from animal and human sources were studied for protein differences by polyacrylamide gel electrophoresis and for antigenic differences by immunoblot analysis. The polyacrylamide gels showed that whilst the isolates were for the most part homogeneous in their protein banding patterns, some isolates did show some differences. The immunoblot analysis yielded many bands, including prominent bands of 32 and 66 kilodaltons. Five of the six isolates that showed differences in protein banding pattern also showed differences in antigenic reactivity. Our findings suggest that differences can be seen with the use of immunoblotting and that this technique is a tool that may be useful for isolate differentiation when used in conjunction with other techniques. Key words: Giardia, giardiasis, characterization, immunoblot.

Electrophoretic evidence supporting self-incompatibility in Lotus corniculatus

1980 ◽  
Vol 58 (6) ◽  
pp. 712-716 ◽  
Author(s):  
Shirley Dobrofsky ◽  
W. F. Grant
Keyword(s):  
Gel Electrophoresis ◽  
Seed Set ◽  
Polyacrylamide Gel Electrophoresis ◽  
Lotus Corniculatus ◽  
Polyacrylamide Gel ◽  
Self Incompatibility ◽  
Banding Patterns ◽  
Protein Subunits ◽  
Self Pollination

Self-incompatibility, a prefertilization event, and self-sterility, a postfertilization event, have both been suggested as causes for differences in seed set between cross- and self-pollinated florets in Lotus corniculatus L. Ovary protein subunits of selfed, crossed, and unpollinated florets of L. corniculatus cv. Mirabel were studied using polyacrylamide gel electrophoresis. Banding patterns differed for all three conditions. Ovary protein differences were found prior to the time fertilization is known to occur, thereby providing evidence that self-incompatibility is at least partially responsible for the reduced seed set after self-pollination.

Isozymes as markers for identification of tissue culture and greenhouse-grown strawberry cultivars

1991 ◽  
Vol 71 (4) ◽  
pp. 1195-1201 ◽  
Author(s):  
N. S. Nehra ◽  
K. K. Kartha ◽  
C. Stushnoff
Keyword(s):  
Tissue Culture ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Meristem Culture ◽  
Banding Patterns ◽  
Extraction Buffer ◽  
Leaf Tissues ◽  
The Stability ◽  
Strawberry Cultivars

Polyacrylamide gel electrophoresis (PAGE) was used for analysis of isozyme banding patterns of leucine aminopeptidase (LAP), phosphoglucomutase (PGM), phosphoglucoisomerase (PGI), esterase (EST) and 6-phosphogluconate dehydrogenase (6-PGD) in strawberry leaves. The extracts prepared from young leaf tissues using polytron homogenization and an extraction buffer containing 15 mg ml−1 dithiothreitol (DTT) and 10% insoluble polyvinylpyrrolidone (PVP-6755) gave best resolution for these enzymes. The influence of plant age and various growing environments on the stability of isozyme phenotypes was examined. The isozyme banding patterns of 6-PGD were found to vary with the change in growing environment as well as age of the plants. EST produced different banding patterns in greenhouse and tissue culture leaves. However, the isozyme phenotypes of LAP, PGM and PGI remained stable under all the conditions tested. Using a combination of these three stable enzymes, it was possible to distinguish eight strawberry cultivars under both tissue culture and greenhouse conditions. Key words: Fragaria × ananassa Duch., meristem culture, polyacrylamide gel electrophoresis

Investigations on the nature of a graft-transmissible agent in poinsettia

1993 ◽  
Vol 71 (8) ◽  
pp. 1097-1101 ◽  
Author(s):  
John M. Dole ◽  
Harold F. Wilkins ◽  
Sharon L. Desborough
Keyword(s):  
Mosaic Virus ◽  
Gel Electrophoresis ◽  
Leaf Morphology ◽  
Rna Viruses ◽  
Polyacrylamide Gel Electrophoresis ◽  
Euphorbia Pulcherrima ◽  
Branching Pattern ◽  
Banding Patterns ◽  
Double Stranded Rna ◽  
Transmission Electron

The free-branching poinsettia (Euphorbia pulcherrima) cultivar Annette Hegg Brilliant Diamond contains a free-branching agent that is graft-transmissible to the restricted-branching cultivar Eckespoint C-1 Red. Transmission electron microscopy failed to reveal evidence of bacteria, fungi, or mycoplasma-like organisms in either 'Brilliant Diamond' or 'C-1 Red' plants. Treatment of both cultivars with tetracycline-hydrochloride produced no differences in branching pattern or leaf morphology in either cultivar, indicating that the agent may not be a mycoplasma-like organism. Scions of a poinsettia mosaic virus indicator species (Euphorbia cyathophora) grafted onto 'Brilliant Diamond' and 'C-1 Red' stocks exhibited the mottling symptoms characteristic of poinsettia mosaic virus, while self-grafted E. cyathophora scions showed no mottling, indicating that poinsettia mosaic virus was not the agent. The agent was not transmitted by pin prick, carborundum, or dodder (Cuscuta sp.), and ribaviran did not eliminate expression of the branching agent from 'Brilliant Diamond' plants. No differences in double-stranded RNA banding patterns were found between extracts of free- and restricted-branching poinsettias by polyacrylamide gel electrophoresis. The double-stranded RNA was attributed to poinsettia mosaic virus and other unknown RNA viruses. Attempts to detect a specific DNA associated with free-branching were inconclusive. Key words: branching agent, Euphorbia pulcherrima, graft transformation, dsRNA, poinsettia mosaic virus.

scholarly journals Organospecific reactions of yellow lupin seedlings to lead

2014 ◽  
Vol 66 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Jarosław Gzyl ◽  
Roman Przymusiński ◽  
Adam Woźny
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Gel Electrophoresis ◽  
Cell Walls ◽  
Polyacrylamide Gel Electrophoresis ◽  
Lupinus Luteus ◽  
Root Tip ◽  
Yellow Lupin ◽  
Transmission Electron ◽  
Slight Amount

Changes caused by lead, supplied in the form of Pb(NO3)2, in roots and hypocotyls of 4 day old yellow lupin (<em>Lupinus luteus</em> L. cv. <em>ventus</em>) seedlings have been analyzed using a transmission electron microscope and polyacrylamide gel electrophoresis (PAGE). The cells of all examined parts of the roots growing in the presence of Pb<sup>2+</sup> contained many lead deposits (mainly in the cell walls and vacuoles) and the increased amount of polypeptides of molecular weight close to 16 kDa have been observed. Similar changes were detected in the area of hypocotyl adjoining the root. However, in upper regions of the hypocotyl only a slight amount of lead deposits was visible and the 16 kDa polypeptide content was comparable to the control cells. The obtained results indicate a relationship between the presence of lead deposits in cells and accumulation of polypeptides of - 16 kDa. The results seem also to indicate that in the analyzed parts of the seedlings, both the amount of accumulated polypeptides of MW - 16 kDa and the amount of lead decreased from root tip to hypocotyl.

scholarly journals Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

2018 ◽  
Vol 19 (5) ◽  
pp. 1683-1689 ◽  
Author(s):  
NUR RAHMAH HIDAYATI ◽  
SURANTO SURANTO ◽  
SAJIDAN SAJIDAN
Keyword(s):  
Banding Pattern ◽  
Polyacrylamide Gel Electrophoresis ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Retardation Factor ◽  
Cucurbita Moschata ◽  
Esterase Isozyme ◽  
Banding Patterns ◽  
Different Altitudes

Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.

GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE): IV. ELECTROPHORETIC BANDING PATTERNS OF OCTANOL DEHYDROGENASE AND ARGININE PHOSPHOKINASE

1979 ◽  
Vol 111 (11) ◽  
pp. 1307-1310 ◽  
Author(s):  
R.H. Gooding ◽  
B.M. Rolseth
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Aldehyde Oxidase ◽  
Glossina Morsitans Morsitans ◽  
Polyacrylamide Gel ◽  
Single Locus ◽  
Single Individual ◽  
Glossina Morsitans ◽  
Banding Patterns ◽  
Octanol Dehydrogenase

AbstractPolyacrylamide gel electrophoresis of the thoraces of adult Glossina morsitans morsitans Westwood revealed five octanol dehydrogenase (ODH, E.C. 1.1.1.73) phenotypes (and a sixth was predicted) in males and females, two arginine phosphokinase (APK, E.C. 2.7.3.3) phenotypes in males and three APK phenotypes in females. Each enzyme was postulated to be under the control of a single locus; Odh with three alleles on an autosome and Apk with two alleles on the X-chromosome. Allele frequencies were in Hardy-Weinberg equilibrium in our colony, and breeding experiments provided direct evidence for single locus control of each enzyme. The polyacrylamide gel electrophoresis technique described permits determination of banding patterns for xanthine oxidase, aldehyde oxidase, ODH, and APK from a single individual.

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