Ascospore development in Ophiostoma piceae
The development of ascoma, ascus, and ascospore in Ophiostoma piceae was studied ultrastructurally and compared with that of other Ascomycetes. Ascospore delimitation commenced with the formation of double delimiting membranes in the ascus. The ascospore wall, consisting of the primary and secondary walls, was deposited between these membranes. The elongate ascospores of O. piceae differed from other species having similarly shaped ascospores, with respect to the shape and arrangement of asci in the ascoma, the number of wall layers of the ascospore, and formation of the secondary wall. Asci in O. piceae are spindle shaped, arranged along the periphery of the ascomatal wall. The ascospores have three layered walls, whereas some other species in Ceratocystis s.l. also with elongated ascospores probably have only two wall layers. Key words: Ophiostoma, elongated ascospores, sheaths, centrum.