Localization of amylase activity in cotyledons of germinated mung bean seeds

1991 ◽  
Vol 69 (7) ◽  
pp. 1501-1506 ◽  
Author(s):  
Yasuko Kaneko ◽  
Hisashi Matsushima ◽  
Yukio Morohashi

Developmental patterns of amylase activity in mung bean (Vigna radiata) cotyledons were observed by histochemical methods. In axis-attached cotyledons, the development of amylase activity and the decompostion of starch grains occurred first in cells located farthest from vascular bundles, and 3 days after imbibition, starch granules appeared to remain intact only in two to three cell layers around the vascular bundles. In axis-detached cotyledons, on the other hand, no conspicuous change in amylolytic activity was detected. When cotyledons were allowed to imbibe water through the surface not covered by the seed coat, amylase activity developed, even if they were detached from the axis. However, the developmental patterns of the activity were quite different from those with attached cotyledons; high amylase activity was detected in the cell layers beneath the epidermis of the surface not covered by the seed coat. These histochemical observations are related to previous results of biochemical experiments. Key words: amylase development, cotyledons, germination, histochemistry, mung bean, Vigna radiata.

2011 ◽  
Vol 74 (8) ◽  
pp. 1224-1230 ◽  
Author(s):  
AMANDA J. DEERING ◽  
ROBERT E. PRUITT ◽  
LISA J. MAUER ◽  
BRADLEY L. REUHS

Escherichia coli O157:H7 has been associated with numerous outbreaks involving fresh produce. Previous studies have shown that bacteria can be internalized within plant tissue and that this can be a source of protection from antimicrobial chemicals and environmental conditions. However, the types of tissue and cellular locations the bacteria occupy in the plant following internalization have not been addressed. In this study, immunocytochemical techniques were used to localize internalized E. coli O157:H7 expressing green fluorescent protein in germinated mung bean (Vigna radiata) hypocotyl tissue following contamination of intact seeds. An average of 13 bacteria per mm3 were localized within the sampled tissue. The bacteria were found to be associated with every major tissue and corresponding cell type (cortex, phloem, xylem, epidermis, and pith). The cortical cells located on the outside of the vascular bundles contained the majority of the internalized bacteria (61%). In addition, the bacteria were localized primarily to the spaces between the cells (apoplast) and not within the cells. Growth experiments were also performed and demonstrated that mung bean plants could support the replication of bacteria to high levels (107 CFU per plant) following seed contamination and that these levels could be sustained over a 12-day period. Therefore, E. coli O157:H7 can be internalized in many different plant tissue types after a brief seed contamination event, and the bacteria are able to grow and persist within the plant.


2020 ◽  
Vol 193 (1) ◽  
pp. 125-139 ◽  
Author(s):  
Ikumi Shinke ◽  
Toru Tokuoka

Abstract Phylogenetic relationships in Styracaceae are well understood, but embryological characters and the ontogeny of integument(s) are still uncertain in many species. The goals of this study are to evaluate the systematic implications of embryological characters in Styracaceae, clarify the character evolution of the number of integuments and suggest a mechanism for the transition between unitegmic and bitegmic ovules. We examined the embryological characters of four genera and five species of Styracaceae, most of which were shared across taxa. However, Styrax has specific embryological features including bitegmic ovules, a multiplicative and sclerotic outer mesotesta and vascular bundles in the testa, all possible autapomorphies. The other three genera of Styracaceae share a unitegmic ovule, a parenchymatous mesotesta and a seed coat without vascular bundles, possible plesiomorphies with Diapensiaceae and Symplocaceae. The transition from a unitegmic to a bitegmic condition can be interpreted to be caused by a downwards shift of the boundary between the inner and outer integument, due to reduced activity in the subdermal initials and increased activity in the dermal initials of the outer integument at its base.


1983 ◽  
Vol 61 (12) ◽  
pp. 3279-3282
Author(s):  
Françoise M. Dumortier ◽  
Jan C. Vendrig

A natural inhibitor of anthocyanin synthesis has been found in seeds of mung beans (Vigna radiata). The inhibitor might play an important role in the regulation of anthocyanin accumulation in seedlings. It is mainly localized in the seed coat and in the cotyledons and is excreted in the incubation medium soon after sowing. Removal of the inhibitor by rinsing the seedlings and transferring them to fresh medium results in higher anthocyanin accumulation as compared with seedlings left in the original incubation medium. Addition of the inhibitor 12 h after its removal does not affect anthocyanin accumulation when the seedlings have been grown in complete darkness. On the other hand, anthocyanin accumulation initiated by irradiation with red light is inhibited under these conditions. The inhibitor has been partially purified and some of its chemical characteristics were determined from its behaviour during purification.


1994 ◽  
Vol 74 (2) ◽  
pp. 335-337
Author(s):  
A. Liptay ◽  
P. Vandierendonck ◽  
A. M. J. Liptay

The exterior of the cylindrical stamens of mung bean [Vigna radiata (L.) Wilczek] flowers are replete with trichomes attached at both ends to the outside periphery of the stamen. The resultant repetitive pattern from below the base of the stamen to the anther resembles a multi-stranded rope configuration. Tests on models with or without simulated doubly attached, trichome-like structures, indicated about a 12-fold increase in stiffness with addition of the trichome-like structures. It is hypothesized that the function of the trichomes on the stamens is to increase their rigidity to ensure successful pollen transfer from the anther as the stigma elongates past the anther. In contrast to the trichomes on the stamen, those on the surface of the style are like a coarse-haired brush apparently designed to collect pollen as the stigma extends past the anthers. Key words: structural strength, stiffness, Vigna radiata


1978 ◽  
Vol 79 (1) ◽  
pp. 10-19 ◽  
Author(s):  
B Baumgartner ◽  
K T Tokuyasu ◽  
M J Chrispeels

Vicilin peptidohydrolase, the protease that hydrolyzes the reserve proteins in the cotyledons of mung bean (Vigna radiata) seedlings, has been localized intracellularly by immunofluorescence microscopy using monospecific antibodies against the enzyme and rhodamine-coupled goat-anti-rabbit immunoglobulin G's. The enzyme can first be visualized after 3 days of seedling growth and is associated with small foci within the cytoplasm of the storage parenchyma cells farthest from the vascular bundles. On the 4th day of growth, the protease is also present in the numerous large protein bodies within these cells. Vicilin peptidohydrolase is known to be synthesized de novo starting on the 3rd day of growth. Our observations are therefore consistent with the interpretation that the enzyme is synthesized in the cytoplasm and subsequently transported to the protein bodies.


1991 ◽  
Vol 69 (3) ◽  
pp. 656-670 ◽  
Author(s):  
Thompson Demetrio Pizzolato

The vascular system of the sessile, fertile spikelet of Sorghum was reconstructed from serial transverse sections. The vascular system is a composite of the basipetal extensions of the traces from the appendages on the rachilla. The rachilla immediately above the glumes consists of an outer and an inner series of these vascular extensions. The basipetal continuations of the median traces of the sterile and fertile lemmas, and of the traces from the stamens comprise the inner series. The outer series consists of the continuations of the many lodicule traces and of vascular bundles descending from the posterior of the pistil. The component of the vascular system related to the pistil is a plexus of xylem and phloem in the form of a hollow cylinder traversed by a large vascular bundle that is the basipetal continuation of the stylar bundles. Bundles from the anterior of the pistil merge with the hollow cylinder at its anterior. Several collateral bundles from the placenta merge with the hollow cylinder at its posterior. Distal portions of these placental bundles supply the short chalaza of the ovule but do not enter it. The vascular system of the fertile spikelet of Sorghum is typical of the Panicoideae, and is useful in distinguishing the Panicoideae from the other subfamilies of grasses. Key words: Sorghum, spikelet, floret, vascular system.


2018 ◽  
Vol 8 (4) ◽  
pp. 65-69
Author(s):  
Mao Nguyen Van ◽  
Dong Tran Nam

Background: Pigmented tumour of the skin is one of the common tumour in human including the benign pigmented tumours (more common) called Nevi tumours and the malignant one called melanoma which was less frequent but the most poor in prognosis. In addition, the others not belonging to these group had the same clinical appearance, so the application of histopathology and immunohistochemistry for the definitive diagnosis was indespensible. Objectives: 1. To describe the macroscopic features of the pigmented tumoral-like lesions; 2. To classify the histopathologic types of the pigmented cell tumours and the other pigmented tumours of the skin. Materials and Method: Cross-sectional research on 55 patients diagnosed as pigmented tumoral lesions by clinician, then all definitively diagnosed by histopathology combining the immunohistochemistry in difficult cases. Results: There was no difference in gender, the disease was discovered most common in adult, especially with the age over 51 years old (58.1%). the most region located was in the face accounting for 60%, following the trunk and limbs (14.6%, 12.8% respectively). All 3 malignant melanomas happened in foot. The most common color of the lesions was black (65.4%), the other ones were rose, grey and blue. Histopathology and immunohisthochemistry showed that the true pigmented cell tumours were 52.6% encompassing benign ones (Nevi tumour) (41.8%), melanoma (5.4%) and lentigo (5.4%). 47.4% was not the true pigmented cell tumour including pigmented basocellular carcinoma (36.4%) and the others less common as histiofibromas, acanthoma and papilloma. Conclusion: the pigmented tumoral-like lesions of the skin could be the true pigmented cell tumours and the others, so the application of the histopathology and the immunohistochemistry after the clinical discovery helps to determine and classify the disease definitely and for the best orientation of treatment as well. Key words: skin tumour, benign pigmented tumour (Nevi), malignant pigmented tumour (melanoma), pigmented basocellular carcinoma


For identification of varieties, it is desirable to use genetically predetermined traits that are decisive for the varietal identification of the distinguishing characteristics of the varieties production and new genotypes for selection. The presence a complete evaluation of the selection samples a particular crop is the basis for the formation of informative databases characteristics the varieties of standards used in plant examination to determine the level of expression a characteristic particular genotype according to the code of their manifestation. For the 20 traits we used to identify, we identified 6 least volatile, highly trait-stable traits over the years of study to identify. These are: “coloring of the pubescence of the main stem”, “the size of the lateral leaf”, “the intensity of green leaf color”, “flower color”, “the intensity of brown bean color”, “basic color of the seed coat”. The other 14 traits are highly variable and unstable among varieties, but may be significant under certain conditions. According to the results of the research we have determined the conformity of the collection varieties by code graduation. In the study, we selected varieties with corresponding codes of attributes of the reference varieties the UPOV technique. On the basic of the color the pubescence of the main stem, 36% varieties are with grey stem pubescence. Among the standards: Apache, Alaric, Talon according to the grey color of the pubescence, we identified varieties Zolotysta, Ozzie, Diona. The 2947 564/84, Stepnaya-90, Knyazhna. The lateral leaf size is a sign that affects the increase in green mass of plants and subsequently on productivity. Graduation of the sign small size of leaf had varieties: Zuma, Ksenya; middle – Heinong 37, Yuh-30; large – 2947 564/84, Vinni, DSS 2504. The following is a sign of the intensity of leaf plate, which is divided by gradation into light, moderate and dark. Among our samples with a light degree of color, we can distinguish Diona, Sribna Ruta, and Zuma. For varieties with moderate gradation of color are selected: Perlyna, 93/99, Knyazhna. To the dark color of the leaf plate we attributed the varieties: Stepnaya-90, Ozzie. Among the varieties we studied of the basics of flower color 37% varieties are with a white flower, and 63% with a purple. According to the varieties of standards: Chandor, Cresir, Toreador to the white color of the flower we attributed varieties Knyazhna, Ozzie, Zolotysta. With the purple color of the flower we have selected varieties: 93/99, Zhemchuzhna, Heinong 37. On the basis of the intensity of brown color bean, which characterizes the generative organs of the plant, is divided into weak, moderate and strong, but the other identifiers there are gradations: sandy, gray, light brown, brown and dark brown, so we decided what would be better identify by color gradation. To the sandy color of the beans we attributed varieties Perlyna, Yuh-30; gray – Sribna Ruta; light brown – Kobza, Knyazhna; brown – Ksenya; dark brown – Zhemchuzhna, Vinni. Important features that identify the description of varieties include basic color of the seed coat, which affects the taste of the seeds. In particular, the seeds are divided by color into yellow – Kobza, Yuh-30; yellow-green – Vinni, Luch Nadezhdy; green – Heorhina; light brown – 2947 564/84, brown – DSS 2504; dark brown – 2974 YS-24 and black color – not detected. Following the results of the previous collection of soybean varieties from the morphological sings of vegetative, generative organs of plants, it was possible to identify varieties with stable manifestation of standard identifying sings of soybean. Collection varieties with one standard characteristics: Heinong 37, Luch Nadezhdy, DSS 2504, 2974 YS-24; two signs: Zolotysta, Diona, Stepnaya-90, Zuma, Ksenya, Heorhina, Sribna Ruta, Perlyna, 93/99, Zhemchuzhna; three signs: Ozzie, 2947 564/84, Yuh-30, Vinni, Knyazhna.


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