Lignification level and peroxidase activity during in vitro rooting of Prunus avium

1989 ◽  
Vol 67 (7) ◽  
pp. 2182-2186 ◽  
Author(s):  
Fabienne Dalet ◽  
Daniel Cornu
Keyword(s):  
Peroxidase Activity ◽  
Prunus Avium ◽  
Polyacrylamide Gel Electrophoresis ◽  
In Vitro Rooting ◽  
Prunus Species ◽  
Wild Cherry ◽  
Rooting Medium ◽  
Series Of Experiments ◽  
The Relationship

To improve the in vitro rooting of selected clones of wild cherry (Prunus avium), the search for a physiological marker for rhizogenesis was initiated. Peroxidase activity is considered as a good rooting marker for other Prunus species. Peroxidase activity was thus assayed by spectrophotometric measurement, and cationic and anionic isoperoxidases were separated by polyacrylamide gel electrophoresis. The measurement of peroxidase activity was made during the first 8 days of subculture on rooting medium with and without auxin (β-indolylbutyric acid). From a first set of experiments, there was no relationship between peroxidase activity and the rooting process. In a second series of experiments, before subculturing on the rooting medium with and without auxin, the explants were divided into three classes according to their lignification level: less, moderately, and more lignified cuttings. Level and changes in peroxidase activity were found to be correlated with the lignification level of the explants. However, there was no effect on final rooting rate. In each class of shoots, the relationship between peroxidase activity and rooting rate, with or without auxin, was highly variable.

scholarly journals Effect of IBA and TIBA on rhizogenesis of Wild cherry in vitro

Topola ◽  
2020 ◽  
pp. 5-11
Author(s):  
Vanja Vuksanović ◽  
Branislav Kovačević ◽  
Lazar Kesić ◽  
Lazar Pavlović ◽  
Erna Vaštag ◽  
...  
Keyword(s):  
Shoot Growth ◽  
Prunus Avium ◽  
In Vitro Cultivation ◽  
Wood Production ◽  
Wild Cherry ◽  
Variation Of Parameters ◽  
Planting Material ◽  
Rooting Medium ◽  
Ecological Importance

Nowadays, considerable attention is paid to wild cherry (Prunus avium L.) due to its economic and ecological importance. This fast-growing noble tree species is highly valued in furniture industry, while preservation and restoration of its genetic variability is closely related to preservation of biodiversity in general. Along with its importance there is high interest in vegetative propagation of interesting wild cherry genotypes, especially by means of tissue culture. This technique provides relatively fast propagation and production of healthy planting material. Obtained plants are used for the establishment and improvement of seed orchards or the establishment of clonal plantations dedicated for wood production. Important phase in micropropagation of wild cherry is rooting. In this work, results of application of indole-3-butyric acid (IBA) and 2,3,5-triiodobenzoic acid (TIBA) in rooting medium are presented, as well as the effect of medium pH. After thirty-five days of in vitro cultivation, results of analysis of variance suggested significant effect of examined treatments on variation of parameters of rooting and survival of wild cherry explants. The best rooting and shoot growth were obtained on treatments based on modified of MS medium with 20 µIBA and pH 5.8.

scholarly journals High Frequency Micropropagation of Aloe vera L. Burm. f. as a Low Cost Option Towards Commercialization

2010 ◽  
Vol 20 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Anusree Das ◽  
Priyanka Mukherjee ◽  
Timir Baran Jha
Keyword(s):  
Low Cost ◽  
Aloe Vera ◽  
In Vitro Rooting ◽  
Shoot Bud ◽  
Rooting Medium ◽  
Bud Induction ◽  
Aloe Gel ◽  
The Cost ◽  
First Time

An efficient micropropagation protocol has been developed using shoot apical meristem as explants in a high barbaloin content 'bitter' cultivar of Aloe vera L. The protocol involves induction, multiplication and in vitro rooting of the regenerated shoots and their acclimation under ex vitro conditions. 35.5 µM BAP and 9.8 µM IBA in combination with 81.4 µM adenine sulphate proved optimum for shoot bud induction. Combination of 8.87 µM BAP and 2.46 µM IBA produced highest number of shoot buds (22.0 ± 0.14) and enhanced bud proliferation within one - two weeks after first subculture. For induction of in vitro rooting, Aloe gel as an alternative to conventional rooting medium used for the first time resulted in 100% rooting and highest number of roots per culture (10.90 ± 0.17). The plantlets were successfully hardened. Cent per cent plants survived in the field condition. Chromosomal analysis of the regenerated plantlets established a stable germplasm with 2n = 14 bimodal chromosomes. The cost effectiveness and economic viability of the protocol has also been evaluated. Key words: Aloe vera, bitter cultivar, micropropagation, cost analysis D.O.I. 10.3329/ptcb.v20i1.5962 Plant Tissue Cult. & Biotech. 20(1): 29-35, 2010 (June)

scholarly journals Polyamines and ethylene during in vitro rooting of Prunus avium L

1989 ◽  
Vol 46 (Supplement) ◽  
pp. 124s-126s
Author(s):  
S. Biondi ◽  
N. Bagni
Keyword(s):  
Prunus Avium ◽  
In Vitro Rooting ◽  
Prunus Avium L

RESPIRATORY ACTIVITY OF OOCYTES ISOLATED FROM OVARIAN FOLLICLES OF THE RAT

1975 ◽  
Vol 78 (4) ◽  
pp. 751-759 ◽  
Author(s):  
T. Hillensjö ◽  
L. Hamberger ◽  
K. Ahrén
Keyword(s):  
Respiratory Activity ◽  
Cellular Respiration ◽  
Cell Complexes ◽  
Corona Radiata ◽  
Exogenous Substrate ◽  
Series Of Experiments ◽  
Prepubertal Rats ◽  
Relationship Of ◽  
The Relationship

ABSTRACT In order to study substrate dependent respiration oocytes were isolated from antrum follicles obtained from ovaries of prepubertal rats. The oocytes used were either surrounded by 1 to 3 layers of granulosa cells (oocyte + corona radiata), or mechanically denuded from surrounding cells (denuded oocytes). By use of Cartesian micro-diver technique the respiratory rate could be determined in single oocyte + corona radiata cell complexes or in samples of a few denuded oocytes. The oocytes were incubated either in an isotonic Tris-HCl buffer or in a hypotonic phosphate buffer. The rate of respiration was determined without addition of exogenous substrate and with addition of different substrates in varying concentrations. Cellular respiration was linear 2–4 h both in absence and presence of substrate. Both types of oocyte preparations were unable to utilize glucose as substrate. Lactate stimulated slightly the respiration of the denuded oocyte while pyruvate and oxalacetate increased markedly the respiratory activity of both types of oocyte prepararations. Succinate had an influence on the oocyte + corona radiata only when the hypotonic medium was used while it was easily utilized in both media by the denuded oocyte. In a preliminary series of experiments the in vitro influence of bovine luteinizing hormone (LH) on the cellular respiration was studied. Both in presence and absence of corona radiata a significant decrease in oxygen uptake was registered, consistently appearing within 30 min after the addition of the hormone. As the relationship of this phenomenon to ovum maturation was not studied, the possible biological meaning remains to be elucidated.

scholarly journals Rooting Medium Composition and In Vitro Rooting and Greenhouse Survival of Five Raspberry Cultivars

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 788C-788
Author(s):  
Becky R. Hughes* ◽  
Wanda J. Cook ◽  
Candy N.F. Keith
Keyword(s):  
Dry Weight ◽  
Root Diameter ◽  
Medium Composition ◽  
Rubus Idaeus ◽  
In Vitro Rooting ◽  
Stem Length ◽  
Indolebutyric Acid ◽  
Rooting Medium ◽  
Plant Stem Length

In vitro rooting and subsequent greenhouse survival of `Autumn Britten', `Boyne', `Comet',`Nova' and `Qualicum' raspberry (Rubus idaeus L.) plantlets were compared following four weeks on a rooting medium with and without activated charcoal, and with 0.1, 0.5, 1.0, 2.0 or 3.0 milligrams per litre IBA. The addition of charcoal significantly increased the percentage of plantlets that produced roots in vitro for the hard-to-root cultivars. Percent rooting in vitro was highest with the three lower levels of IBA. Root number was influenced only by the cultivar, while root diameter and length were affected by all the factors investigated. Greenhouse survival was affected by the cultivar, the presence or absence of charcoal and the IBA level in the in vitro rooting medium, with significant interactions. Provided charcoal was present in the rooting medium, the level of IBA didn`t alter survival. The addition of charcoal to the rooting medium improved greenhouse survival of the three hardest-to-root cultivars. Plug plant stem length; internode length and dry weight were increased by the presence of charcoal in the in vitro rooting medium for all but the easiest to establish cultivar. Chemical names used: 3-indolebutyric acid (IBA).

scholarly journals Morphogenesis in vitro and peroxidase activity in barley cv. Steptoe and its ABA-deficient mutant AZ34: effects of inhibitors of ABA synthesis and auxin transport

2020 ◽  
Author(s):  
O. A. Seldimirova ◽  
G. R. Kudoyarova ◽  
I. R. Galin ◽  
D. S. Veselov ◽  
N. N. Kruglova
Keyword(s):  
Peroxidase Activity ◽  
Auxin Transport ◽  
Deficient Mutant ◽  
Barley Mutant ◽  
Morphogenesis In Vitro ◽  
The Relationship ◽  
Aba Synthesis

The relationship between the effect of ABA on morphogenesis in vitro and auxin transport, as well as the role of peroxidases in the action of ABA on morphogenesis in vitro in the ABA-deficient barley mutant AZ34 and its parent form cv. Steptoe was studied.

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