Effect of sodium fluoride treatments on the mitotic cycle of root-tip cells in jack pine seedlings

1989 ◽  
Vol 67 (5) ◽  
pp. 1606-1608 ◽  
Author(s):  
J. J. Zwiazek ◽  
Jennifer M. Shay
Keyword(s):  
Sodium Fluoride ◽  
Pinus Banksiana ◽  
Mitotic Cycle ◽  
Jack Pine ◽  
Root Tip ◽  
Pine Seedlings ◽  
Induced Changes ◽  
Tip Cells ◽  
Accumulation Method ◽  
Root Tip Cells

Fluoride-induced changes in the mitotic cycle were studied in root-tip cells of jack pine (Pinus banksiana Lamb.) seedlings using the metaphase accumulation method. Sodium fluoride treatments resulted in lengthening of the mitotic cycle. This was due to an increase in the duration of interphase. The duration of mitosis and individual mitotic phases was not affected by the treatments.

MITOTIC CYCLES IN ROOT TIP CELLS OF TWO SPECIES OF TRILLIUM (LILIACEAE)

1970 ◽  
Vol 12 (4) ◽  
pp. 750-758 ◽  
Author(s):  
E. R. Boothroyd ◽  
Nancy MacDonald Mark
Keyword(s):  
Mitotic Cycle ◽  
The Other ◽  
Root Tip ◽  
Cycle Times ◽  
Autoradiographic Technique ◽  
Tip Cells ◽  
Root Tip Cells

There is a fourfold difference in the reported lengths of the mitotic cycles in Trillium. One report (29 hours at 23 °C for T. erectum) was based on the colchicine tag method, whereas the other (120 hours at 25 °C for T. grandiflorum) was based on the autoradiographic technique. In the present study both methods were used on both species. Experiments, carried out at 20 °C, gave minimum estimates of 104 hours and 84 hours respectively for T. grandiflorum and T. erectum, as determined by the colchicine method, and 92 hours and 80 hours by autoradiography. Both methods gave evidence of the existence of two or more populations of cells with different mitotic cycle times.

Sunlight decreased genotoxicity of azadirachtin on root tip cells of Allium cepa and Eucrosia bicolor

2010 ◽  
Vol 73 (5) ◽  
pp. 949-954 ◽  
Author(s):  
W. Kwankua ◽  
S. Sengsai ◽  
C. Kuleung ◽  
N. Euawong
Keyword(s):  
Allium Cepa ◽  
Root Tip ◽  
Tip Cells ◽  
Root Tip Cells

Salt Stress-induced Programmed Cell Death in Rice Root Tip Cells

2007 ◽  
Vol 49 (4) ◽  
pp. 481-486 ◽  
Author(s):  
Jian-You Li ◽  
Ai-Liang Jiang ◽  
Wei Zhang
Keyword(s):  
Cell Death ◽  
Salt Stress ◽  
Programmed Cell Death ◽  
Rice Root ◽  
Root Tip ◽  
Tip Cells ◽  
Root Tip Cells

Cytological evidence bearing on the origin of the B genome in polyploid wheats

Genome ◽  
1988 ◽  
Vol 30 (1) ◽  
pp. 36-43 ◽  
Author(s):  
K. Kerby ◽  
J. Kuspira
Keyword(s):  
Dna Hybridization ◽  
Triticum Turgidum ◽  
Root Tip ◽  
Cytological Evidence ◽  
B Genome ◽  
Genome Donor ◽  
The One ◽  
Genome Donors ◽  
Tip Cells ◽  
Root Tip Cells

To help elucidate the origin of the B genome in polyploid wheats, karyotypes of Triticum turgidum, Triticum monoccum, and all six purported B genome donors were compared. The analysis utilized a common cytological procedure that employed the most advanced equipment for the measurement of chromosome lengths at metaphase in root tip cells. A comparison of the karyotypes of T. turgidum and T. monococcum permitted the identification of B genome chromosomes of T. turgidum. These consist of two SAT pairs, one ST pair, three SM pairs, and one M pair of homologues. Comparisons of the chromosomes of the B genome of T. turgidum with the karyotypes of the six putative B genome donors showed that only the karyotype of Aegilops searsii was similar to the one deduced for the donor of the B genome in T. turgidum, suggesting that Ae. searsii is, therefore, the most likely donor of the B genome to the polyploid wheats. Support for this conclusion has been derived from geographic, DNA-hybridization, karyotype, morphological, and protein data reported since 1977. Reasons why the B genome donor has not been unequivocally identified are discussed.Key words: phylogeny, karyotypes, Triticum turgidum, Triticum monococcum, B genome, B genome donors.

scholarly journals Squash Preparations of Living Root-Tip Cells

Nature ◽  
1949 ◽  
Vol 164 (4178) ◽  
pp. 930-930 ◽  
Author(s):  
J. CHAYEN
Keyword(s):  
Root Tip ◽  
Living Root ◽  
Tip Cells ◽  
Root Tip Cells

The effect of cytochalasin D on preprophase band organization in root tip cells of Allium

1992 ◽  
Vol 103 (4) ◽  
pp. 989-998 ◽  
Author(s):  
E.P. Eleftheriou ◽  
B.A. Palevitz
Keyword(s):  
Preprophase Band ◽  
Cytochalasin D ◽  
Root Tip ◽  
Average Width ◽  
Exact Position ◽  
The Relationship ◽  
Tip Cells ◽  
Root Tip Cells ◽  
Control Samples

The relationship between microfilaments (Mfs) and microtubules (Mts) in the organization of the preprophase band (PPB) was investigated in Allium root tip cells subjected to treatment with cytochalasin D (CD). Mts and Mfs were visualized by indirect immunofluorescence and various parameters such as PPB width were analyzed quantitatively. In control samples, the PPB first appears as a wide Mt band that progressively narrows to an average width of 4 micrometre in mid-prophase. Randomly oriented Mfs are present throughout the cytoplasm of most interphase control cells. Preprophase and prophase cells, however, contain cortical Mfs arranged parallel to the PPB. The Mfs initially occupy much of the cortex but in most cells they progressively become restricted to an area wider than the PPB. In the presence of CD, the PPB fails to narrow and remains at least two-fold wider than in control cells. PPB width expressed as a percentage of nuclear or cell length also increases compared to controls. Widening is concentration dependent, and the effect of 10 micromolar CD is near maximal only 15 min after application of the drug. This rapid response suggests that a rebroadening of already condensed PPBs takes place. After as little as 15 min in CD, Mfs are replaced by many small specks and rods. Dual localizations of both Mts and Mfs show that prophase cells contain broad PPBs without Mfs. The rapid disorganization of Mfs, by CD, therefore coincides with the rebroadening of PPBs. CD-treated cells in metaphase, anaphase and telophase contain larger actin aggregates at the poles, as previously reported. The results indicate that Mfs play an important role in the narrowing of the PPB, which in turn is essential for determination of the exact position of the plane of division. They also indicate that movement of intact Mts is important in PPB organization.

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