Isozyme and general protein patterns of Armillaria spp. collected from the boreal mixedwood forest of Ontario

1989 ◽  
Vol 67 (4) ◽  
pp. 1143-1147 ◽  
Author(s):  
D. Lin ◽  
M. T. Dumas ◽  
M. Hubbes
Keyword(s):  
Total Protein ◽  
Geographic Area ◽  
Biological Species ◽  
Distinct Pattern ◽  
Protein Profiles ◽  
Esterase Isozyme ◽  
Protein Patterns ◽  
Boreal Mixedwood Forest ◽  
General Protein ◽  
Esterase Patterns

Isolates of four North American biological species of Armillaria, I (A. ostoyae), III, V, and VII (A. lutea), that occur in the boreal mixedwood forest of Ontario were differentiated by their esterase isozyme patterns and total protein profiles. Within biological species I, there are two types of esterase patterns that correspond to the genotypically distinct clones observed in isolates from the same geographic area. In addition, isolates of biological species I from different geographic areas showed differences in their esterase patterns. The general protein patterns of four biological species of Armillaria isolated from maple also showed the distinct pattern of each intersterility group.

scholarly journals 238 Isoperoxidase and Protein Patterns in Compatible and Incompatible Pear/Quince Graft Combinations

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 483B-483
Author(s):  
Hatice Gulen ◽  
Chon C. Lim ◽  
Rajeev Arora ◽  
Hatice Gulen ◽  
Ali Kuden ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Protein Profiles ◽  
Protein Patterns ◽  
Graft Union ◽  
Peroxidase Isozymes ◽  
Sds Page ◽  
Starch Gel ◽  
Graft Compatibility ◽  
General Protein

The similarity or differences of peroxidase isozymes in rootstocks and scions may influence their graft compatibility. This study was conducted to identify peroxidase isozymes and/or other proteins that may be used as markers to predict compatibility between pear and various quince clones. `Bartlett' (BT) and `Beurre Hardy' (BH) pear cultivars were budded on 13 selected quince clones and quince A (QA) rootstocks; BT and BH cultivars are known to be incompatible and compatible, respectively, with quince root stocks. Bark and cambial tissues were taken from unbudded rootstocks, scions, and 4 cm above and below the graft union for isozyme analysis. Samples were collected 1, 2, 3, and 12 months after grafting. In addition, samples from the graft unions were also analyzed 12 months after grafting. Isozyme separation was performed by starch gel electrophoresis. Many isozyme bands were commonly observed in the two scions; however, one anodal peroxidase was detected in BH but not in BT samples. This isozyme was also detected in QA and in all but four quince clones. Protein profiles of bark tissues from QA and three pear scions (BT, `Bosc', and P. crassane) were determined using SDS-PAGE. In general, protein profiles of the three pear cultivars appeared remarkably similar; however, P. crassane (a compatible pear cultivar on QA) had a 63 kDa protein, which was absent in BT and faintly observed in `Bosc' (intermediate compatibility). Our results suggest that these isoperoxidase and polypeptide could be associated with pear/quince graft compatibility.

scholarly journals CyDye Immunoblotting for Proteomics: Co-detection of specific immunoreactive and total protein profiles

PROTEOMICS ◽  
2006 ◽  
Vol 6 (24) ◽  
pp. 6400-6404 ◽  
Author(s):  
Pamela M. Donoghue ◽  
Ciara A. McManus ◽  
Niaobh M. O'Donoghue ◽  
Stephen R. Pennington ◽  
Michael J. Dunn
Keyword(s):  
Total Protein ◽  
Protein Profiles ◽  
Co Detection

scholarly journals Biogenesis of endoplasmic reticulum proteins involved in Ca2+ signalling during megakaryocytic differentiation: an in vitro study

2000 ◽  
Vol 350 (3) ◽  
pp. 723-734 ◽  
Author(s):  
Christine LACABARATZ-PORRET ◽  
Sophie LAUNAY ◽  
Elisabeth CORVAZIER ◽  
Raymonde BREDOUX ◽  
Béla PAPP ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Western Blotting ◽  
Time Course ◽  
In Vitro Study ◽  
Fold Increase ◽  
Distinct Pattern ◽  
Platelet Glycoprotein ◽  
Protein Patterns ◽  
Fold Induction

The endoplasmic reticulum (ER) plays a key role in Ca2+ signalling through Ca2+ release via inositol 1,4,5-trisphosphate receptors (InsP3-Rs) and Ca2+ uptake by sarco/endoplasmic reticulum Ca2+-ATPases (SERCAs). Here, we investigated the organization of platelet ER and its biogenesis during megakaryocytopoiesis. First, erythro/megakaryoblastic MEG 01, UT7, M-O7e and CHRF 288-11 cell lines, platelets and thrombopoietin-induced UT7-Mpl cells were selected for the study of SERCA2b and SERCA3 proteins by Western blotting using the antibodies IID8 and PL/IM430, respectively. As judged by platelet glycoprotein IIIa (GPIIIa) expression, an increase in SERCA3 proteins was observed while that of SERCA2b remained unchanged throughout maturation. Second, these studies were extended to the newly described alternatively spliced SERCA3a–c RNAs and InsP3-Rs using the in vitro model of PMA-induced differentiation of MEG 01 cells. Time-course and dose–response studies showed a maximal approx. 4-fold up-regulation of SERCA3 proteins using 10-8 M PMA for 3 days, which paralleled induction of GPIIIa expression. SERCA3 induction was found to occur at the level of mRNA. The modulation of the different SERCA3 species (i.e. 3a, 3b and 3c) was isoform-specific: while SERCA3a was slightly increased, an approx. 3-fold induction of SERCA3b, and a 4-fold induction of SERCA3c, was observed after 24h of PMA treatment. Isoform-specific Western blotting and/or reverse transcriptase PCR studies showed that InsP3-R types I, II and III are expressed in MEG 01 cells, as well as in platelets. Study of the expression of these InsP3-R types in PMA-induced MEG 01 cells revealed that: (i) InsP3-RI protein and mRNA showed no changes; (ii) InsP3-RII mRNA was up-regulated and peaked at hour 48 and (iii) InsP3-RIII mRNA and protein showed a transitory maximal 3- and 2.3-fold increase at hours 6 and 30, respectively. Upon PMA treatment of CHRF 288-11 cells, in which GPIIIa is not induced upon treatment, a similar pattern of regulation of InsP3-R types II and III was seen, but a distinct pattern of SERCA3 regulation was observed. These results suggest a profound reorganization of ER-protein patterns during megakaryocytopoiesis and underline the role of SERCA3 gene regulation in the control of Ca2+-dependent platelet functions.

Isoelectric focusing of general protein and specific enzymes from pathotypes of Globodera rostochiensis and G. pallida

Parasitology ◽  
1984 ◽  
Vol 88 (1) ◽  
pp. 131-139 ◽  
Author(s):  
P. C. Fox ◽  
H. J. Atkinson
Keyword(s):  
Isoelectric Focusing ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Species Variation ◽  
Glucose Phosphate Isomerase ◽  
Protein Patterns ◽  
Major Band ◽  
Glucose Phosphate ◽  
General Protein ◽  
And Cluster Analysis

SUMMARYVariations in the general protein and 40 enzymes of the 8 European pathotypes of the potato cyst nematode Globodera rostochiensis and G. pallida have been examined by isoelectric focusing (IEF) and cluster analysis. Differences in the protein patterns were observed and the presence of a major band at pH 8·0 allowed the two species to be discriminated. Extra isozymed of peptidase (EC 3.4. 11.*) and esterase (EC 3. 1. 1. 1) were also found in G. rostochiensis. Intra-species variation was seen within G. rostochiensis for superoxide dismutase (EC 1. 15. 1. 1) and within G. pallida for phosphoglucomutase (EC 2. 7. 5. 1) and glucose phosphate isomerase (EC 5. 3. 1. 9). The results suggest that the two species are very similar and the prospect for recognizing their pathotypes by IEF is discussed.

Vegetative compatibility groups and protein electrophoresis indicate a role for basidiospores in spread of Inonotus tomentosus in spruce forests of British Columbia

1991 ◽  
Vol 69 (8) ◽  
pp. 1756-1763 ◽  
Author(s):  
Katherine J. Lewis ◽  
Everett M. Hansen
Keyword(s):  
British Columbia ◽  
Key Words ◽  
Picea Glauca ◽  
Protein Electrophoresis ◽  
Vegetative Compatibility ◽  
Protein Profiles ◽  
Vegetative Compatibility Group ◽  
Protein Patterns ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group

The importance of spore infection in the spread of Inonotus tomentosus was assessed using vegetative compatibility and protein electrophoresis. Isolates were collected from diseased spruce (Picea glauca × engelmannii) trees from five sites. Each site had several small (two or three trees) discrete disease centres, or larger patchy centres, or both. Within each site, the vegetative compatibility group and protein profiles of isolates were examined in all combinations of paired isolates. Vegetatively compatible isolates had identical protein profiles in 74% of the comparisons. Vegetatively incompatible isolates had different protein profiles 97% of the time. Usually isolates differed by only one or two protein bands. Isolates from a discrete centre were usually vegetatively compatible with identical protein patterns. Larger patchy centres consisted of multiple vegetatively compatible groups. The number of unique vegetatively compatible groups found suggests that spores are an important course of infection. Key words: vegetative compatibility, disease centre, protein electrophoresis.

Major biological activities and protein profiles of skin secretions of Lissotriton vulgaris and Triturus ivanbureschi

2018 ◽  
Vol 43 (6) ◽  
pp. 605-612 ◽  
Author(s):  
Mert Karış ◽  
Doğancan Şener ◽  
Hüsniye Tansel Yalçın ◽  
Ayşe Nalbantsoy ◽  
Bayram Göçmen
Keyword(s):  
Total Protein ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Protein Profiles ◽  
Skin Secretion ◽  
Active Components ◽  
Lissotriton Vulgaris ◽  
Hemolytic Effect ◽  
Skin Secretions

Abstract Objective The aim of this study was to determine the total protein amounts, protein profiles, in vitro cytotoxicities, antimicrobial activities and hemolytic effects of skin secretions of the Lissotriton vulgaris and Triturus ivanbureschi. Methods Skin secretions were obtained, clarified, supernatants snap-frozen then lyophilized. Total protein amounts were determined by BCA assay kit. Protein profiles were revealed by the SDS-PAGE. The cytotoxicity and antimicrobial activity were determined by using MTT assay and minimum inhibitory concentration (MIC) method. Hemolytic effects were measured on rabbit red blood cells. Results Lissotriton vulgaris and T. ivanbureschi skin secretions have totally 18 and 20 protein fractions. IC50 values were detected between 1.40 and 40.28 μg/mL. The MIC results were found between 7.8 and 250 μg/mL. Lissotriton vulgaris skin secretion showed low hemolytic effect while T. ivanbureschi skin secretion showed high hemolytic effect. Conclusion This study is the first report showing the potential of L. vulgaris and T. ivanbureschi skin secretions for cytotoxicity, antimicrobial and hemolytic activity as an alternative therapeutic approach for traditional uses. Further studies need to focus on purification of the active components from these skin secretions and mode of action on cancer cell lines and microorganisms as anti-agents.

scholarly journals Assessment of Salivary and Serum Proteins in Patients with Oral Tumors

2013 ◽  
Vol 10 (3) ◽  
pp. 934-944
Author(s):  
Baghdad Science Journal
Keyword(s):  
Total Protein ◽  
Healthy Subjects ◽  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Albumin Concentration ◽  
Protein Patterns ◽  
Age And Gender ◽  
Oral Tumors ◽  
And Gender ◽  
Sera Samples

The qualified subjects for this study included 33 patients with benign and malignant oral tumors aged 15-75 years and 31 matched age and gender healthy subjects used as control. Proteins measurements included total protein, albumin, globulines in sera and saliva samples, and immunoglobulins (IgG, IgM, IgA) in sera samples of control and patients. Meanwhile, polyacrylamide gel electrophoresis (PAGE) was used to differentiate between protein patterns in both serum and saliva samples among the studied groups. The gel was also stained for glycoprotein to evaluate as well the changes in glycoprotein contents. For total protein, the results revealed a signifigant increase (P?0.01) in both samples (serum and saliva) of patient group. Albumin concentration shows presence of a high significant decrease (P=0.001) in sera samples but a significant increase (P

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