scholarly journals CyDye Immunoblotting for Proteomics: Co-detection of specific immunoreactive and total protein profiles

PROTEOMICS ◽  
2006 ◽  
Vol 6 (24) ◽  
pp. 6400-6404 ◽  
Author(s):  
Pamela M. Donoghue ◽  
Ciara A. McManus ◽  
Niaobh M. O'Donoghue ◽  
Stephen R. Pennington ◽  
Michael J. Dunn
Keyword(s):  
Total Protein ◽  
Protein Profiles ◽  
Co Detection

Isozyme and general protein patterns of Armillaria spp. collected from the boreal mixedwood forest of Ontario

1989 ◽  
Vol 67 (4) ◽  
pp. 1143-1147 ◽  
Author(s):  
D. Lin ◽  
M. T. Dumas ◽  
M. Hubbes
Keyword(s):  
Total Protein ◽  
Geographic Area ◽  
Biological Species ◽  
Distinct Pattern ◽  
Protein Profiles ◽  
Esterase Isozyme ◽  
Protein Patterns ◽  
Boreal Mixedwood Forest ◽  
General Protein ◽  
Esterase Patterns

Isolates of four North American biological species of Armillaria, I (A. ostoyae), III, V, and VII (A. lutea), that occur in the boreal mixedwood forest of Ontario were differentiated by their esterase isozyme patterns and total protein profiles. Within biological species I, there are two types of esterase patterns that correspond to the genotypically distinct clones observed in isolates from the same geographic area. In addition, isolates of biological species I from different geographic areas showed differences in their esterase patterns. The general protein patterns of four biological species of Armillaria isolated from maple also showed the distinct pattern of each intersterility group.

Major biological activities and protein profiles of skin secretions of Lissotriton vulgaris and Triturus ivanbureschi

2018 ◽  
Vol 43 (6) ◽  
pp. 605-612 ◽  
Author(s):  
Mert Karış ◽  
Doğancan Şener ◽  
Hüsniye Tansel Yalçın ◽  
Ayşe Nalbantsoy ◽  
Bayram Göçmen
Keyword(s):  
Total Protein ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Protein Profiles ◽  
Skin Secretion ◽  
Active Components ◽  
Lissotriton Vulgaris ◽  
Hemolytic Effect ◽  
Skin Secretions

Abstract Objective The aim of this study was to determine the total protein amounts, protein profiles, in vitro cytotoxicities, antimicrobial activities and hemolytic effects of skin secretions of the Lissotriton vulgaris and Triturus ivanbureschi. Methods Skin secretions were obtained, clarified, supernatants snap-frozen then lyophilized. Total protein amounts were determined by BCA assay kit. Protein profiles were revealed by the SDS-PAGE. The cytotoxicity and antimicrobial activity were determined by using MTT assay and minimum inhibitory concentration (MIC) method. Hemolytic effects were measured on rabbit red blood cells. Results Lissotriton vulgaris and T. ivanbureschi skin secretions have totally 18 and 20 protein fractions. IC50 values were detected between 1.40 and 40.28 μg/mL. The MIC results were found between 7.8 and 250 μg/mL. Lissotriton vulgaris skin secretion showed low hemolytic effect while T. ivanbureschi skin secretion showed high hemolytic effect. Conclusion This study is the first report showing the potential of L. vulgaris and T. ivanbureschi skin secretions for cytotoxicity, antimicrobial and hemolytic activity as an alternative therapeutic approach for traditional uses. Further studies need to focus on purification of the active components from these skin secretions and mode of action on cancer cell lines and microorganisms as anti-agents.

scholarly journals Evaluation of Non-climacteric Senescence of Gladiolus sp. Flowers

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 760A-760
Author(s):  
G.H. Pemberton ◽  
Terril A. Nell ◽  
James E. Barrett
Keyword(s):  
Total Protein ◽  
Acc Synthase ◽  
Total Protein Content ◽  
Protein Profiles ◽  
Flower Longevity ◽  
Sds Page ◽  
Specific Proteins ◽  
Postharvest Life ◽  
Relationship Of ◽  
The Relationship

Senescence of gladiolus flowers, like many geophytes, does not involve a climacteric burst of ethylene. Eleven gladiolus cultivars were screened and all were non-climacteric (NC) for both respiration and ethylene production. Average ethylene levels for individual flowers were 0.5 μl C2H4/kg per h or less. As in other NC flowers, protein synthesis may be linked to senescence. Our goal was to identify specific proteins that were involved in the senescence process that could be used as indicators of postharvest longevity. SDS-PAGE protein profiles of cut gladiolus flowers were determined from a tight bud stage to senescence. Both increases and decreases were observed in major polypeptides that may be connected to postharvest flower longevity. Total protein content of gladiolus flower petals decreased by ≈70% during the profile period. This could explain the relatively short postharvest life of 3 to 5 days for individual gladiolus flowers. Total protein profiles were probed with an ACC synthase antibody to establish the relationship of this enzyme in NC senescence.

scholarly journals MODULATION OF PROTEIN LEVELS IN CHROMOSOMAL DOSAGE SERIES OF MAIZE: THE BIOCHEMICAL BASIS OF ANEUPLOID SYNDROMES

Genetics ◽  
1981 ◽  
Vol 99 (2) ◽  
pp. 247-266
Author(s):  
James A Birchler ◽  
Kathleen J Newton
Keyword(s):  
Structural Gene ◽  
Total Protein ◽  
Gene Dosage ◽  
Negative Influence ◽  
Chromosome 1 ◽  
Activity Levels ◽  
Protein Profiles ◽  
Gene Dosage Effect ◽  
Biochemical Basis ◽  
Gene Dosage Effects

ABSTRACT Genetically defined dosage series of chromosome arms 1L, 3L, 4S, 5L, 7L, 9S, 10L and combinations of 1L-3L, collectively spanning approximately one-third of the maize genome, were examined for alterations in the expression of total protein profiles in scutellar tissue. The major effects found were negative correlations of specific proteins with the dosage of particular regions in a manner similar to that previously described for enzyme activity levels (Birchler 1979). Chromosome arms 1L, 4S and 5L produced the most severe negative effects, with 3L and 7L exhibiting this phenomenon to a lesser degree. Positive correlations of certain proteins were observed with the dosage of the 1L, 3L, 5L and 7L regions. The structural locus of one of the major scutellar proteins (PRO) is present in the long arm of chromosome 1 (Schwartz 1979), but exhibits compensation in a dosage series involving whole-arm comparisons. Multiple factors in 1L affect the level of the protein. The compound TB-1La-3L4759-3 (1L 0.20-0.39) has a slight negative effect on PRO, while TB-1La-3Le (1L 0.20-0.58) and TB-1La-3L5267 (1L 0.20-0.72) have a more pronounced negative influence. The level of this protein is not altered by the dosage of 3L. These observations suggest that compensation is brought about by the cancellation of a positive structural gene dosage effect by the negative inverse effect. Other regions of the genome that contribute to the control of PRO levels are 4S and 5L. Total protein profiles were also compared in haploid, diploid and tetraploid maize as a comparison to the aneuploid series. Most proteins exhibit structural-gene-dosage effects through the ploidy series, but others show a positive effect greater than expected from varying the structural genes. Still others are negatively affected by ploidy changes. In general, the ploidy alterations are not as great as predicted from the cumulative action of the aneuploid effects. The bearing of these observations on the biochemical basis of aneuploid syndromes is discussed.

scholarly journals Serum protein profiles and C-reactive protein in natural canine filariasis

2021 ◽  
Vol 14 (4) ◽  
pp. 860-864
Author(s):  
Sariya Asawakarn ◽  
Sujin Sirisawadi ◽  
Nanthida Kunnasut ◽  
Patchana Kamkong ◽  
Piyanan Taweethavonsawat
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Dirofilaria Immitis ◽  
Reference Range ◽  
Buffy Coat ◽  
Detection Methods ◽  
Protein Profiles ◽  
C Reactive Protein ◽  
Normal Reference Range ◽  
Reactive Protein

Background and Aim: Canine filariasis is caused by several species of filarial worms. The pathophysiological response to infection is mainly due to the filaria lifecycle. Laboratory detection methods to assess the pathological alterations characteristic of filariasis are needed urgently. Serum protein profiles and C-reactive protein (CRP) levels are used widely to diagnose several animal diseases. This study aimed to determine the serum protein profiles and CRP levels in dogs infected with Dirofilaria immitis or Brugia pahangi or both parasites. Materials and Methods: Blood samples were collected from 980 dogs presenting at animal hospitals and veterinary clinics in Bangkok and its vicinity. The presence of microfilaria in samples was determined using a buffy coat smear and staining with Wright–Giemsa. The sheathed and unsheathed microfilaria species were identified by acid phosphatase staining. Forty positive samples were tested. The serum protein profiles were identified by agarose gel electrophoresis. The CRP concentration was measured using a fluorescent immunoassay. Results: Albumin levels and albumin-to-globulin ratios were significantly lower, and total protein, β2 globulin, and γ globulin levels were significantly elevated in dogs infected with D. immitis and B. pahangi compared with reference values in normal dogs. The average CRP concentrations in dogs infected with D. immitis or B. pahangi were 69.9 and 12.9 mg/L, respectively. Conclusion: The total protein and γ globulin levels increased in canine filariasis compared with the normal reference range. The CRP concentration in dogs infected with D. immitis was extremely high, whereas that in dog infected with B. pahangi was normal.

scholarly journals Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles

2015 ◽  
Vol 18 (1) ◽  
pp. 58
Author(s):  
Sri Darmawati ◽  
Langkah Sembiring ◽  
Widya Asmara ◽  
Wayan T. Artama ◽  
Syaiful Anwar
Keyword(s):  
Key Words ◽  
Total Protein ◽  
Reference Strain ◽  
Protein Profile ◽  
Blood Cultures ◽  
Protein Profiles ◽  
E Coli ◽  
Relationship Analysis ◽  
Pair Group ◽  
Sds Page

The purpose of this study was to determine the chemosystematic of 14 strains of bacteria in blood cultures from Semarang using 1 reference strain S. typhi NCTC 786, based on the total protein profi les with the similarity relationship analysis based on Simple Matching Coeffi cient (SSM) analysis and algorithm methodof unweighted pair group with averages (UPGMA) presented in a dendrogram. The results showed that thechemosystematic based on the total protein profi les using SDS-PAGE method can classify the member ofbacterial strains of each species. The Clusters respectively consist of 4 strains of S. typhi (similarity: 89.7%),2 strains of Ser. marcescens (similarity: 89.7%), two strains of E. coli, and one strain of Salmonella ssp, S. typhi NCTC 786 (similarity: 100%). Those three incorporated clusters had the similarity value of 75.3%. Those four strains of Ent. cloacae composed in one cluster (similarity: 100%) are incorporated in a cluster consisting of one strain of Kleb. pneumoniae (similarity: 92.9%). Both clusters were incorporated in a cluster consisting of S. typhi NCTC 786 (similarity: 67.9%). Key words: Enterobacteriaceae, chemosystematic, blood cultures, protein profile

Changes in total protein profiles of barley cultivars in response to toxic boron concentration

2000 ◽  
Vol 23 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Homa Mahboobi ◽  
Meral Yucel ◽  
Huseyin Avni Öktem
Keyword(s):  
Total Protein ◽  
Boron Concentration ◽  
Protein Profiles ◽  
Barley Cultivars
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