Cryptic seed abortion and the estimation of ovule fertilization

1987 ◽  
Vol 65 (11) ◽  
pp. 2463-2465 ◽  
Author(s):  
Robert R. Nakamura ◽  
Maureen L. Stanton

In a natural population of Raphanus sativus L. 41% of all fertilized ovules aborted before seed maturation. However, in mature fruits over 75% of the abortion was undetected. The cryptic abortion of developing seeds was likely due to the early time of abortion and the corky nature of the pericarp. The results are important for the accurate measurement of ovule fertilization and seed abortion in ecological studies of plant reproduction.

1983 ◽  
Vol 38 (1-2) ◽  
pp. 21-27 ◽  
Author(s):  
Dieter Strack ◽  
Wolfgang Knogge ◽  
Brigitte Dahlbender

A cell-free system from developing seeds of red radish (Raphanus sativus L. var. sativus) catalyzed the transfer of the sinapoyl moiety of 1-O-sinapoyl-β-ᴅ-glucose to choline to form O-sinapoylcholine (sinapine). A wide range of proportionality with respect to incubation time and protein concentration was found in the determination of this enzymatic activity using a high performance liquid chromatographic (HPLC) method. The transacylase activity showed an absolute acceptor specificity towards choline and a high hydroxycinnamoyl donor specificity with 1-sinapoylglucose. Maxim|al rate of sinapine formation was found to be around pH 7.0 in 0.08 M potassium phosphate buffer. The apparent Km values were 0.30 mм for 1-sinapoylglucose and 7.64 mм for choline. Change in extractable transacylase activities from different stages of seed development correlated well with the in vivo accumulation kinetics of sinapine. Maximal activity around 36 pkat seed-1 and 129μkat kg-1 protein was extracted from dark-green immature seeds, which are in the process of rapid sinapine accumulation. About 20% of this activity still has been found in dark-brown mature seeds. This enzymatic acitivity refers to an enzyme which can be classified as 1-sinapoylglucose: choline sinapoyltransferase (SCT).


Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
LLR Fiorucci ◽  
GC Mantovanelli ◽  
DI Bernardi ◽  
AA Silva ◽  
RS de Oliveira Jr ◽  
...  

2019 ◽  
Vol 48 (8) ◽  
pp. 811-822
Author(s):  
Dawun Yang ◽  
Seunghyun Hong ◽  
Hak Sung Lee ◽  
Nam Ho Lee ◽  
Taekyun Shin ◽  
...  

Gene ◽  
2021 ◽  
pp. 145753
Author(s):  
Chen Liu ◽  
Weimin Fu ◽  
Wenling Xu ◽  
Xianxian Liu ◽  
Shufen Wang

1986 ◽  
Vol 35 (3) ◽  
pp. 223-231 ◽  
Author(s):  
J. Derksen ◽  
G. Jeucken ◽  
J. A. Traas ◽  
A. A. M. van Lammeren

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