Early grain development of the seg2 maternal-effect shrunken-endosperm mutant of barley

1987 ◽  
Vol 65 (5) ◽  
pp. 943-948 ◽  
Author(s):  
Frederick C. Felker ◽  
David M. Peterson ◽  
Oliver E. Nelson

Barley (Hordeum vulgare L.) plants that are homozygous for the seg2 mutation produce shrunken seeds with 15% of normal dry weight regardless of pollen source. Microscopical examination of developing grains sampled from 1 to 8 days after anthesis revealed abnormal endosperm development in seg2 compared with normal barley. Antipodal cells degenerated between 2 and 4 days after anthesis in normal grains, and the amount of endosperm cytoplasm increased and became cellular by 4 days after anthesis. Antipodals persisted beyond 4 days after anthesis in seg2, and the endosperm cytoplasm did not change in appearance or amount until 8 days after anthesis, when thin lobes of endosperm cells developed in the lateral regions. No central endosperm developed in seg2, but rather dorsal nucellar tissue was appressed directly against the ventral nucellar projection, eliminating the usual endosperm cavity. Maternal and embryo tissue of seg2 appeared to develop normally, although the rate of embryo growth was slower than in the normal cultivar. We suggest that seg2 barley would be useful as a genetic probe to study maternal control of endosperm development or antipodal function.

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1482
Author(s):  
Silvia Pampana ◽  
Alessandro Rossi ◽  
Iduna Arduini

Winter cereals are excellent candidates for biosolid application because their nitrogen (N) requirement is high, they are broadly cultivated, and their deep root system efficiently takes up mineral N. However, potential N leaching from BS application can occur in Mediterranean soils. A two-year study was conducted to determine how biosolids affect biomass and grain yield as well as N uptake and N leaching in barley (Hordeum vulgare L.), common wheat (Triticum aestivum L.), durum wheat (Triticum turgidum L. var. durum), and oat (Avena byzantina C. Koch). Cereals were fertilized at rates of 5, 10, and 15 Mg ha−1 dry weight (called B5, B10, and B15, respectively) of biosolids (BS). Mineral-fertilized (MF) and unfertilized (C) controls were included. Overall, results highlight that BS are valuable fertilizers for winter cereals as these showed higher yields with BS as compared to control. Nevertheless, whether 5 Mg ha−1 of biosolids could replace mineral fertilization still depended on the particular cereal due to the different yield physiology of the crops. Moreover, nitrate leaching from B5 was comparable to MF, and B15 increased the risk by less than 30 N-NO3 kg ha−1. We therefore concluded that with specific rate settings, biosolid application can sustain yields of winter cereals without significant additional N leaching as compared to MF.


2017 ◽  
Vol 9 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Faride BEHBOUDI ◽  
Zeinalabedin TAHMASEBI SARVESTANI ◽  
Mohamad Zaman KASSAEE ◽  
Seyed Ali Mohamad MODARES SANAVI ◽  
Ali SOROOSHZADEH

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.  


1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.


1988 ◽  
Vol 66 (2) ◽  
pp. 388-393 ◽  
Author(s):  
P. Pukittayacamee ◽  
A. K. Hellum

Germination of Acacia auriculiformis A. Cunn. ex Benth. seeds was related to seed development. Full physiological development of seeds, indicated by maximum seed dry weight, was reached 82 days after anthesis; however, maximum percent germination was not reached before day 89. Later, germination declined gradually as dormancy and mortality increased. Most seeds were capable of germination without pretreatment at the time of collection, indicating that seed coats were not impermeable to water. Germination of seeds with moisture content from 14 to 29% can be achieved. Anatomical studies revealed that seeds reached maturity after compressing the parenchyma cells against the inside of the seed coat. The physical properties of the seed coat, therefore, did not control its permeability to water. After the developmental period, seed dormancy increased by further drying of seeds during storage.


1990 ◽  
Vol 17 (4) ◽  
pp. 431 ◽  
Author(s):  
CY Caley ◽  
CM Duffus ◽  
B Jeffcoat

The mechanism of temperature regulation of endosperm development has been investigated by studying the effects of two temperature regimes on starch deposition and starch synthase activity during grain development in two cultivars of wheat. Most of the starch synthase activity was present throughout development as the granule-bound form using ADPglucose as the principal substrate. That starch synthase may be a rate-limiting enzyme for accumulation of starch, and hence dry weight, is suggested by: (1) rates are proportionately less in the cultivar with the lower final endosperm dry weight; (2) at elevated temperatures when starch content and dry weight are reduced, starch synthase activity falls; (3) the rate of starch deposition calculated to be possible from measured rates of starch synthase activity is close to the observed rate of starch deposition. On the other hand, it was concluded that it is not lack of starch synthase activity that causes termination of starch deposition, since activity is maintained well after starch deposition has ceased. Using the same two wheat cultivars, grown as detached ears in liquid culture, the effects of reduced endosperm water content, induced by the presence of polyethylene glycol in the culture medium, were investigated. Endosperm starch synthase activity was unaffected but ADPglucose pyrophosphorylase activity was greatly reduced, suggesting a possible role in the termination of starch synthesis.


2014 ◽  
Vol 47 (1) ◽  
pp. 23-29
Author(s):  
S.A. Tabatabaei

ABSTRACT In order to investigate salinity stress on seed reserve utilization and seedling growth of treated seeds of barley (Hordeum vulgare L.), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replicates. To create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, -12 and -16 bar were used. For seed priming, gibberellin (GA) 50 ppm was used. Our results showed that treatment × drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were attained from priming by gibberellin at control conditions. Thus, priming increased characteristics as compared to the unprimed. Priming improved seed reserve utilization such as: weight of utilized (mobilized) seed reserve, seed reserve depletion percentage, seed reserve utilization efficiency and seedling growth in barley under salinity stress.


1982 ◽  
Vol 98 (2) ◽  
pp. 347-355 ◽  
Author(s):  
R. W. Daniels ◽  
M. B. Alcock ◽  
D. H. Scarisbrick

SUMMARYPre-anthesis stem reserve contribution to grain yield was assessed in two spring barley cultivars of contrasting height. It was greatest in the taller, but final grain yields were similar. Partitioning of total reserve capacity to various plant parts showed that the leaf and sheath below the peduncle were most important, followed by stem internodes which had increasing reserve capacity up to internode 4.It is suggested that stem reserves are valuable for yields commonly achieved in U.K. commercial practice.High grain yields were associated with large positive increases in stem dry weight after anthesis. This would indicate that the source capacity to boost yield is more than proportional to that required to fill the grain alone.


2008 ◽  
Vol 56 (8) ◽  
pp. 651 ◽  
Author(s):  
Nabil M. Ahmad ◽  
Peter M. Martin ◽  
John M. Vella

Microsporogenesis, embryogeny and endosperm development of Lomandra longifolia Labill. are described in detail. The formation of the anther wall is the basic type composed of four cell layers, namely an epidermis, an endothecium, one middle layer and a tapetum. The tapetum layer has glandular, uninucleate cells. Successive cytokinesis follows meiosis, subsequently forming a tetrahedral tetrad of microspores. The ovule in each carpel is hemitropous, crassinucellate and bitegmic, with the micropyle formed by the inner integument. The archesporial cell divides periclinally to form the primary parietal and primary sporogenous cells. The sporogenous cell functions as the megaspore mother cell, whereas the parietal cell divides to give rise to two parietal layers. The mature megagametophyte, which has enlarged synergids and antipodals, is of the Polygonum type, with the normal complement of seven cells and eight nuclei. Nucellar tissue in the mature ovule consists of enlarged dermal cells and irregular subdermal cells surrounding a central strand of markedly smaller cells. Endosperm development is of the nuclear type. Embryo development is of the Graminad type, characterised by oblique zygotic and early pro-embryonic divisions.


1976 ◽  
Vol 86 (3) ◽  
pp. 603-608 ◽  
Author(s):  
T. J. Riggs ◽  
P. G. Gothard

SummaryGrains from ears of known anthesis time in seven spring barley cultivars were measured for dry weight and α-amylase activity at regular intervals during grain maturation. During the period 10–31 days after anthesis, dry weight increase of the grain was found to be substantially linear in all the cultivars. Comparisons between linear slopes fitted for this phase of growth were found to provide an objective means of comparing grain growth rates in different cultivars.α-Amylase activity per grain reached a peak in all except one cultivar at between 10 and 16 days after anthesis but declined rapidly during the linear phase of grain growth. α-Amylase activity per gram grain dry weight decreased exponentially during this period and transformation of the data to logarithms allowed a substantially linear fit to be made. Slopes for each of the cultivars were compared.Differences were found between cultivars in grain growth rates, total α-amylase activity and rates of fall of α-amylase activity per gram for the period 10–31 days after anthesis. No relationship could be found between grain growth rate and either the absolute level of α-amylase activity achieved in the grain or the rate of fall in activity during development.


2020 ◽  
Vol 13 (1) ◽  
pp. 21
Author(s):  
DEVI RUSMIN ◽  
MELATI, S MELATI, S ◽  
WAHYUNI WAHYUN ◽  
SUKARMAN SUKARMAN

ABSTRAK<br />Salah satu faktor yang berpengaruh terhadap viabilitas benih<br />sambiloto (Andrographis paniculata Nees) adalah waktu panen.<br />Berdasarkan permasalahan tersebut kegiatan ini dilakukan dengan tujuan<br />untuk mengetahui pengaruh umur panen terhadap viabilitas benih serta<br />hubungannya dengan produksi terna sambiloto. Percobaan dilakukan di<br />KP. Cimanggu dan Laboratorium, Balai Penelitian Tanaman Obat dan<br />Aromatik, dari Maret 2005 – Maret 2006. Percobaan disusun dalam<br />rancangan acak kelompok (RAK) dengan 10 perlakuan stadia umur<br />panen dan 4 ulangan. Stadia umur panen yang diuji yaitu 18, 21, 22, 23,<br />24, 25, 26, 27, 28, dan 29 hari setelah antesis (HSA). Variabel yang<br />diamati yaitu mutu benih (daya berkecambah benih, kecepatan<br />berkecambah), pertumbuhan tanaman (tinggi tanaman, jumlah cabang),<br />serta produksi terna (bobot basah tanaman, bobot kering daun, dan bobot<br />kering batang). Hasil penelitian menunjukkan bahwa: (1) Umur panen<br />benih berpengaruh terhadap daya berkecambah benih dan kecepatan<br />berkecambah benih sambiloto; daya berkecambah dan kecepatan<br />berkecambah tertinggi didapatkan pada umur panen benih 22 dan 21 HSA<br />(67,00 dan 55,00)%; sedangkan daya berkecambah yang terendah<br />diperoleh pada umur panen 18 HSA (23,50)%, (2) Umur panen benih<br />berpengaruh terhadap tinggi tanaman dan jumlah cabang pada tanaman<br />umur 1 bulan. Tinggi tanaman dan jumlah cabang tertinggi berturut-turut<br />didapatkan pada perlakuan umur panen benih 27 dan 26 HSA (39, 63<br />dan 36,58 cm serta 16,71 dan 16,61 buah); dan (3) Umur panen benih<br />berpengaruh terhadap produksi terna (bobot basah tanaman, bobot kering<br />daun, bobot kering batang) pada umur 3 bulan. Bobot basah tanaman,<br />bobot kering daun, serta bobot kering batang tertinggi didapatkan pada<br />perlakuan umur panen benih 27 HSA (291,25, 28, 27 dan 28,86)g. Bobot<br />basah tanaman, bobot kering daun, serta bobot kering batang terendah<br />didapatkan pada perlakuan 18 HSA (217,09, 22,10 dan 20,24)g. Umur<br />panen benih tidak berpengaruh terhadap jumlah cabang pada umur 3<br />bulan.<br />Kata kunci : Sambiloto, Andrographis paniculata Nees, umur panen,<br />viabilitas, produksi, Jawa Barat<br />ABSTRACT<br />The influence of harvesting time on the seed viability and<br />the  relationship  with  herb  yield  of  king  bitter<br />(Andrographis paniculata Nees)<br />One of the main factors influencing the viability of king bitter<br />(Andrographis paniculata Nees) is appropriate harvesting time. Based on<br />this problem a research was conducted to study the relationship between<br />seed maturity and seed viability, and herb yield of king bitter. The research<br />was conducted at Cimanggu Experimental Station and in the laboratory of<br />Indonesian Medicinal and Aromatic Crops Research Institute (IMACRI)<br />from March 2005 to March 2006. The experiment was arranged in a<br />randomized block design with 10 seed maturity stages and 4 replications.<br />Maturity seed tested was 18, 21, 22, 23, 24, 25, 26, 27, 28 and 29 days<br />after anthesis. The observations were made on seed quality (percentage of<br />seed germination, and rate of seed germination), plant growth (plant<br />height, number of branches) and herb yield (fresh herb weight, leaf dry<br />weight, and stem dry weight). The results of experiment indicated that (1)<br />seed maturity affected seed germination, and rate of seed germination of<br />king bitter; the highest seed germination and germination rate were<br />achieved by seeds harvested at 21 and 22 days after anthesis, (2) stage of<br />seed harvesting affected on the plant height and number of branches at 1<br />month after planting. The highest plant height and number of branches<br />were found on the treatments of seeds harvested at 26 and 27 days after<br />anthesis, they were 39.63 and 36.58 cm and 16.71 and 16.61; (3) seed<br />maturity also affected herb production, such as wet weight of plants, dry<br />weight of leaf and dry weight of steam at 3 moths after planting. Wet<br />weight of plant, dry weight of leaf and dry weight of stem were achieved<br />at the treatments of seeds harvested at 27 days after anthesis, they were<br />291.25, 28.27 and 28.86 g. The lowest of wet of plant weight, dry weight<br />of leaf and dry weight of stem were found on the seeds harvested at 18<br />days after anthesis. They were 217.09, 22.10 and 20.24 g. Moreover, the<br />stage of harvesting did not influence the number of branches at 3 months<br />after planting.<br />Key words: King bitter, Andrographis paniculata Nees, seed maturity,<br />viability, production, West Java


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