Heterosis and the metabolism of [3H]gibberellin A1 in maize

1986 ◽  
Vol 64 (9) ◽  
pp. 2160-2164 ◽  
Author(s):  
S. B. Rood
Keyword(s):  
Retention Time ◽  
High Performance ◽  
Zea Mays L ◽  
Specific Activity ◽  
High Specific Activity ◽  
Reversed Phase ◽  
Single Cross ◽  
Parental Inbred ◽  
Labelled Compound ◽  
Gibberellin A1

Seedling growth and the metabolism of high specific activity (1.1 TBq mmol−1) [3H]gibberellin A1 were studied in two Canadian maize (Zea mays L.) inbreds, CM7 and CM49, and in their single cross F1 hybrid. As previously reported, the hybrid grew more rapidly than either parental inbred. Metabolism of the [3H]GA1 was qualitatively similar in all three genotypes and [3H]-labelled metabolites were tentatively identified through sequential, step-elution silicic acid partition chromatography followed by reversed-phase C18 high-performance liquid chromatography, relative to the retention times of authentic GAs. Although the expected 2β-hydroxylation product, [3H]GA8 was not detected, a metabolite was observed at the retention time of GA8-O(2)-glucoside. Additionally, another metabolite(s) eluted at the retention time of glucosyl conjugates of GA1, and enzymic cleavage with cellulase yielded a [3H]-labelled compound which subsequently eluted at the retention time of free GA1. While the ratio of the precursor [3H]GA1 to total [3H]GA conjugate-like metabolites was similar in the three genotypes, the hybrid contained higher levels of the [3H]GA8-glucosidelike metabolite, whereas the inbreds contained higher levels of the [3H]GA1 conjugatelike metabolite(s). Thus, there are differential rates of GA1 metabolism in a maize hybrid relative to its slower-growing inbred parents.

VALIDATED RP-HPLC METHOD FOR DETERMINATION OF ENZALUTAMIDE IN BULK DRUG AND PHARMACEUTICAL DOSAGE FORM

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (11) ◽  
pp. 46-50
Author(s):  
Z. G Khan ◽  
◽  
S. S. Patil ◽  
P. K. Deshmukh ◽  
P. O. Patil
Keyword(s):  
Retention Time ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Uv Detector ◽  
Chromatography Method ◽  
Pharmaceutical Dosage Form ◽  
Bulk Drug

Novel, isocratic reversed phase high performance liquid chromatography method was developed and validated for the determination of enzalutamide (EZA) in bulk drug and pharmaceutical formulation. Efficient separation was achieved on PrincetonSPHER C18 100A, 5μ (250×4.6 mm) under the isocratic mode of elution using acetonitrile: water (80:20) % V/V as a mobile phase pumped in to the column at flow rate 1.0 mL/min. The effluent was monitored at 237.0 nm using UV detector. EZA was eluted in the given mobile phase at retention time (tR) of 3.2 minutes. The standard calibration curve was linear over the concentration range 10 - 60 μg/mL with correlation coefficient 0.997. The method was validated for accuracy, precision, sensitivity, robustness, ruggedness and all the resulting data treated statistically. The system suitability parameters like retention time, theoretical plates, tailing factor, capacity factor were found within the limit.

scholarly journals Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound Lotion

2013 ◽  
Vol 2013 ◽  
pp. 1-4
Author(s):  
Suying Ma ◽  
Haixia Lv ◽  
Xiaojun Shang
Keyword(s):  
Retention Time ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Ionization Detector ◽  
Uv Detector ◽  
Flame Ionization ◽  
Liquid Chromatographic

A high performance liquid chromatographic (HPLC) method with UV detector for the determination of dyclonine hydrochloride and a gas chromatography (GC) method with flame ionization detector (FID) for the determination of camphor and menthol in lotion were developed. The developed HPLC method involved using a SinoChoom ODS-BP C18reversed-phase column (5 μm, 4.6 mm × 200 mm) and mobile phase consisting of acetonitrile : water : triethylamine in a ratio of 45 : 55 : 1.0; pH was adjusted to 3.5 with glacial acetic acid. The developed GC method for determination of camphor and menthol involved using an Agilent 19091J-413 capillary chromatographic column (30 m × 320 μm × 0.25 μm). The two methods were validated according to official compendia guidelines. The calibration of dyclonine hydrochloride for HPLC method was linear over the range of 20–200 μg/mL. The retention time was found at 6.0 min for dyclonine hydrochloride. The calibration of camphor and menthol of GC method was linear over the range of 10–2000 μg/mL. The retention time was found at 2.9 min for camphor and 3.05 min for menthol. The proposed HPLC and GC methods were proved to be suitable for the determination of dyclonine hydrochloride, camphor, and menthol in lotion.

Effects of erythropoietin on uridine metabolism in cell cultures of fetal calf liver

1984 ◽  
Vol 62 (2-3) ◽  
pp. 143-149 ◽  
Author(s):  
L. F. Congote
Keyword(s):  
Cell Cultures ◽  
High Performance ◽  
Specific Activity ◽  
Control Cell ◽  
Fold Increase ◽  
Reversed Phase ◽  
Uridine Incorporation ◽  
Soluble Cell ◽  
Cell Extracts ◽  
Chain Synthesis

The effect of sheep plasma erythropoietin preparations on the incorporation of [5-3H]uridine into erythroid cells has been studied using cells of fetal calf liver cultured in serum-free medium. The cells were incubated for 20 h with the hormone, followed by a 1-h incubation with [3H]uridine. Erythropoietin caused a 2.5-fold increase in the incorporation of uridine into cold trichloroacetic-acid-insoluble cell extracts and a 70% increase in the incorporation of uridine into the cold acid-soluble cell extracts. The phosphorylated metabolites of labeled uridine present in the cold acid-soluble fraction were analyzed by anion-exchange high performance liquid chromatography (HPLC). Erythropoietin increased the amounts of labeled UDP and UTP per cell. However, the specific activity of UTP and the labeled amounts of UDP-glucose in erythropoietin-treated cells were not significantly different from those in control cell cultures. After chromatography of the crude erythropoietin preparations on reversed-phase and gel-permeation HPLC, there was a perfect coincidence of the fractions stimulating uridine incorporation into acid-soluble and acid-insoluble cell extracts. The protein fractions from crude erythropoetin which stimulated uridine incorporation after purification by reversed-phased HPLC were also able to stimulate globin chain synthesis in fetal calf liver cells. These experiments suggest that the multiple effects on uridine metabolism described above are due to erythropoietin, rather than other proteins contaminating the crude hormone preparations.

scholarly journals Identification of Flavonoids from the Leaves Extract of Mangrove (Rhizophora apiculata)

2019 ◽  
Vol 5 ◽  
pp. 1
Author(s):  
Asma Nisar ◽  
Awang Bono ◽  
Hina Ahmad ◽  
Ambreen Lateef ◽  
Maham Mushtaq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
High Performance Liquid Chromatography ◽  
Gallic Acid ◽  
Retention Time ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Rhizophora Apiculata ◽  
Medicinal Properties

A fast and specific reversed-phase high-performance liquid chromatography (HPLC) method was used for the immediate identification of flavonoids (gallic acid, rutin, quercetin, ascorbic acid, and kaempferol) in the leaves extract of Mangrove (Rhizophora apiculata). The R. apiculata has lots of valuable medicinal properties including antiallergic, anti-inflammatory, antimicrobial, antiviral, antioxidant, vascular antitumor activity, and enzyme inhibition; however, the activity of antioxidant is perhaps the greatest studied property attributed to flavonoids. Magnetic stirrer was used for the pretreatment process of sample with methanol by using a temperature of 50°C for 40 min, followed by separation on column size 250 mm x 4.6 mm (5 μm) Hypersil Gold C18 (Thermo Electron Corporation) with water–methanol–acetonitrile (45:40:15 v/v/v) containing acetic acid 1.0% as a mobile phase. Moreover, 254-nm wavelength was used to detect the extract. The standard retention times (Rt) of gallic acid, rutin, ascorbic acid, quercetin, and kaempferol were found to be 2.610, 2.875, 3.150, 5.789, and 8.983, respectively. The existence of gallic acid, rutin, ascorbic acid, kaempferol, and quercetin in Mangrove leaves extract was found matching according to the standard retention time. In Mangrove leaves, gallic acid was found to have the retention time at 2.538, rutin at 2.873, quercetin at 5.796, and kaempferol at 8.976. However, the ascorbic acid was not identified. The amount of rutin, gallic acid, quercetin, and kaempferol was calculated by using the assay formula. In Mangrove leaves, the amount of gallic acid, rutin, quercetin, and kaempferol is 3.024, 5.485, 5.144, and 8.361%, respectively.

scholarly journals VALIDATION OF AN ANALYTICAL METHOD FOR THE QUANTIFICATION OF GIBERELLIC ACID IN MAIZE SEEDS (Zea mays L.) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

2020 ◽  
Vol 25 (1) ◽  
pp. 95-111
Author(s):  
Juan D Rivera ◽  
Javier Torres ◽  
Yaned M Correa-Navarro
Keyword(s):  
Zea Mays ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Gibberellic Acid ◽  
Analytical Method ◽  
High Performance ◽  
Zea Mays L ◽  
Reversed Phase ◽  
Chromatography Method ◽  
Relative Standard

Gibberellic acid is a phytohormone that triggers the germination of seeds in a state of dormancy. Through the quantification of this hormone, the physiological condition of seeds of economic importance can be studded. In this work we validated a High-Performance Liquid Chromatography method to quantify gibberellic acid in germinated maize (Zea mays L.) seeds. Chromatographic conditions included the use of a C-18 reversed-phase column, acetonitrile-formic acid (1 : 9 %) as the mobile phase, flow of 0.5 mL·min-1, and detection at 195 nm. We evaluated our method for seven analytical parameters. The method was linear for gibberellic acid concentrations from1.0 mg·kg-1 to 50.0 mg·kg-1. The method’s limits were 0.3 mg·kg-1 and1.0 mg·kg-1 for detection and quantification, respectively. The method was highly precise; we obtained variable but low relative standard deviations (2.62 % - 12.66 %) for the studied gibberellic acid concentrations. We assessed accuracy through recovery percentages, ranging from 52.85 % - 63.68 %, for three gibberellic acid concentrations. We conclude that our analytical method can be used to measure gibberellic acid during the early stages of maize germination. In addition, the method could be used for the analysis of other types of plant matrices.

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