Identification of pedigrees of hybrid maize (Zea mays L.) cultivars by isozyme electrophoresis and reversed-phase high-performance liquid chromatography

Euphytica ◽  
1988 ◽  
Vol 39 (3) ◽  
pp. 199-205 ◽  
Author(s):  
J. S. C. Smith
Keyword(s):  
Zea Mays ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Zea Mays L ◽  
Reversed Phase ◽  
Isozyme Electrophoresis ◽  
Hybrid Maize

scholarly journals VALIDATION OF AN ANALYTICAL METHOD FOR THE QUANTIFICATION OF GIBERELLIC ACID IN MAIZE SEEDS (Zea mays L.) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

2020 ◽  
Vol 25 (1) ◽  
pp. 95-111
Author(s):  
Juan D Rivera ◽  
Javier Torres ◽  
Yaned M Correa-Navarro
Keyword(s):  
Zea Mays ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Gibberellic Acid ◽  
Analytical Method ◽  
High Performance ◽  
Zea Mays L ◽  
Reversed Phase ◽  
Chromatography Method ◽  
Relative Standard

Gibberellic acid is a phytohormone that triggers the germination of seeds in a state of dormancy. Through the quantification of this hormone, the physiological condition of seeds of economic importance can be studded. In this work we validated a High-Performance Liquid Chromatography method to quantify gibberellic acid in germinated maize (Zea mays L.) seeds. Chromatographic conditions included the use of a C-18 reversed-phase column, acetonitrile-formic acid (1 : 9 %) as the mobile phase, flow of 0.5 mL·min-1, and detection at 195 nm. We evaluated our method for seven analytical parameters. The method was linear for gibberellic acid concentrations from1.0 mg·kg-1 to 50.0 mg·kg-1. The method’s limits were 0.3 mg·kg-1 and1.0 mg·kg-1 for detection and quantification, respectively. The method was highly precise; we obtained variable but low relative standard deviations (2.62 % - 12.66 %) for the studied gibberellic acid concentrations. We assessed accuracy through recovery percentages, ranging from 52.85 % - 63.68 %, for three gibberellic acid concentrations. We conclude that our analytical method can be used to measure gibberellic acid during the early stages of maize germination. In addition, the method could be used for the analysis of other types of plant matrices.

Development and Validation of A Reversed Phase-High Performance Liquid Chromatography Method for the Quantitative Estimation of Ramipril Drug Content from Formulated Dosage Form

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Uv Detector ◽  
Chromatography Method ◽  
Drug Content

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.

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