In vitro control of caulogenesis by growth regulators and media components in embryonic explants of eastern white pine (Pinus strobus)

1986 ◽  
Vol 64 (9) ◽  
pp. 1948-1956 ◽  
Author(s):  
Barry S. Flinn ◽  
David T. Webb ◽  
Wanda Georgis
Keyword(s):  
Callus Formation ◽  
Pinus Strobus ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Vertical Orientation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Isopentenyl Adenine ◽  
The Difference

Horizontally oriented embryos of Pinus strobus (L.) produced shoots on Schenk and Hildebrandt medium containing cytokinin. Shoots developed primarily from cotyledons in contact with the medium. Seed pretreatments at 5 or 27 °C did not affect caulogenesis. N6-Benzyladenine (BA) and N6-(Δ2-isopentenyl)adenine (2iP) both induced caulogenesis, with BA being 10–20 times more potent than 2iP. High BA levels caused callus formation. BA exposures from 1 to 8 weeks were equally caulogenic with horizontal explants, but exposures longer than 2 weeks led to increased variability and callus formation. A 1-week, upside-down, vertical orientation during BA treatment increased the uniformity of cotyledon response and was as caulogenic as a 4-week horizontal BA exposure. Neither auxins nor triiodobenzoic acid induced or significantly enhanced shoot formation. Full-strength Schenk and Hildebrandt medium was superior to Murashige and Skoog medium for shoot induction. Dilution of Schenk and Hildebrandt medium had no significant effect on shoot production, but shoot elongation was suppressed on one quarter strength Schenk and Hildebrandt medium. Half-strength Murashige and Skoog medium was as caulogenic as Schenk and Hildebrandt medium. The NH4 level of the macronutrients was responsible for the difference between Schenk and Hildebrant medium and Murashige and Skoog medium. The higher NH4 concentration of Murashige and Skoog medium inhibited shoot formation.

scholarly journals Future outlook of plant growth regulators application influencing callus induction from different type explant Hyoscyamus muticus L. in vitro

2018 ◽  
Vol 7 (3) ◽  
pp. 10-13
Author(s):  
Walla Abdelmaksood Abdelazeez ◽  
Landysh Zavdetovna Khusnetdinova ◽  
Olga Arnoldovna Timofeeva
Keyword(s):  
Callus Induction ◽  
Nutrient Medium ◽  
Callus Formation ◽  
Stem Explants ◽  
Hyoscyamus Muticus ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Naphthylacetic Acid ◽  
Egyptian Henbane

The article shows the results concerning the problem of the influence of the hormonal composition of the medium on callus induction in isolated from different explants of Egyptian henbane areas (on the example of Hyoscyamus muticus L.). The authors study 11 variants of Murashige and Skoog medium supplemented with different concentrations and combination of auxins and cytokinins. It was important to find nutrient medium modification of Murashige and Skoog for callus induction. The article describes the fact that callus formation from different explant types of Hyoscyamus muticus L. in vitro was observed on Murashige and Skoog medium fortified with benzylaminopurine and naphthylacetic acid. It shows that the maximum callus induction was observed from root explants on Murashige and Skoog's medium supplemented with 0.5 mg/l of benzylaminopurine and 1.0 mg/l of naphthylacetic acid. And minimal callus formation was observed in the area with benzylaminopurine. Callus induction of leaf and stem explants both on the hormone-free nutrient medium and with the benzylaminopurine only was not observed. Thus, the results show that the frequency of callus formation with culturing root segment is higher compared to leaf and stem segment explants (on the example of Egyptian henbane in culture in vitro ). This work aims to inducing callus formation from various explants of Egyptian henbane, which can be used for plant regeneration or as a source for in vitro production of secondary metabolites.

Induction of calusogenesis in a technical (industrial) hemp in in vitro

2018 ◽  
pp. 21-28
Author(s):  
Serhiy Mishchenko
Keyword(s):  
In Vitro Culture ◽  
Shoot Formation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Green Callus ◽  
Industrial Hemp ◽  
Hypocotyl Segments

Separate elements of a technique for introducing hemp into an in vitro culture have been developed. The best option for inducing calusogenesis in technical (industrial) hemp among the studied genotypes in vitro is Murashige and Skoog medium with the addition of 0,5 or 0,3 mg/l 2,4-D, 0,3 mg/l KIN, 0,5 mg/l GA3, vitamins B1, B6, C and 30 g/l sucrose. In this embodiment, the frequency of calusogenesis was 88,5–100%, the formation of green callus with meristematic zones was observed in 73,1–76,5% of the hypocotyl segments, and in some cases organogenesis (shoot formation) also occurred.

scholarly journals Micropropagation of Yellow Passionfruit by Axillary Bud Proliferation

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1276-1277 ◽  
Author(s):  
Joao L.C. Faria ◽  
Juan Segura
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Apices ◽  
Passiflora Edulis ◽  
Multiple Shoot Formation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

A protocol for in vitro propagation in yellow passionfruit (Passiflora edulis F. flavicarpa Deg) has been developed. Shoot apices from aseptically grown seedlings were used as initial explants. Multiple shoot formation was obtained by placing the explants on solidified Murashige and Skoog medium containing BA. Regenerated shoots were rooted on media without growth regulators. Following conventional procedures, plantlets were transferred to soil with more than 90% success. Chemical name used: N-(phenylmethyl)-lH-purin-6-amine (BA).

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

scholarly journals In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

1970 ◽  
Vol 17 ◽  
pp. 139-144 ◽  
Author(s):  
MS Rahman ◽  
MF Hasan ◽  
R Das ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

scholarly journals Essential factors for in vitro regeneration of rose and a protocol for plant regeneration from leaves 

2018 ◽  
Vol 45 (No. 2) ◽  
pp. 83-91
Author(s):  
Anber Mahmoud Ahmed Hassanein ◽  
Inas Mohamed Ali Mahmoud
Keyword(s):  
Indole Acetic Acid ◽  
Culture Medium ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Shoot Elongation ◽  
Favorable Effect ◽  
Indole Butyric Acid ◽  
Leaf Discs ◽  
Better Than

In vitro propagation of Rosa hybrida, L. cv. ‘Eiffel Tower’ was improved by the addition of thidiazuron (TDZ) and silver nitrate (AgNo<sub>3</sub>) to the culture medium. The combination of auxin and cytokinins was indispensable for inducing response from leaf discs. Maintaining cultures under dark was better than light for callus formation and quality. The source of explants was vital in the regeneration process wherein situ explants produced callus while, in vitro explants regenerated somatic embryos and shoots. Gibberellic acid (GA<sub>3</sub>) had a favorable effect where in vitro explants showed somatic embryogenesis with no shoots on media containing TDZ however, 37% of explants regenerated shoots directly on medium containing GA<sub>3</sub>. The presence of benzyl adenine (BA) was essential for shoot elongation, and indole butyric acid (IBA) was better than indole acetic acid (IAA) for rooting. The optimum conditions produced rooted plants from leaf discs within ten weeks. The reported results clarify factors controlling in vitro regeneration of R. hybrida, and provide a rapid protocol allowing further improvements of rose. 

scholarly journals Response of in vitro propagated fig (Ficus carica L.) shoots to the concentrations of benzyl amino purine and coconut water

2021 ◽  
Vol 922 (1) ◽  
pp. 012067
Author(s):  
Sophia ◽  
M Hayati ◽  
E Kesumawati
Keyword(s):  
Callus Formation ◽  
Formation Rate ◽  
Water Concentration ◽  
Shoot Formation ◽  
Coconut Water ◽  
Contamination Rate ◽  
Two Factors ◽  
Number Of Leaves ◽  
Benzyl Amino Purine

Abstract In this study, several concentrations of benzyl amino purine (BAP) and coconut water (CW) were investigated along with the interaction between two factors to the growth of in vitro propagated fig shoots. The investigated factors consisted of BAP concentration: 0, 1, 3, 5 mg L−1 and coconut water concentration: 0, 100, 200, 300 ml L−1. A total of 16 treatment combinations with 6 replications resulting in 96 experimental units consisting of a single fig shoot explant per culture medium. The observed parameters including living explant rate, contamination rate, browning rate, day of first shoot emergence, shoot formation rate, explant height addition, number of leaves, callus formation rate, and number of roots were conducted every week from 1 to 8 weeks after proliferation (WAP). The result indicated that in 8 WAP, the living explant rate reached 23.95%. The combination of concentration 200 ml L−1 CW and 3 mg L−1 BAP + 200 ml L−1 CW-induced early emergence of new shoots at 7 days after proliferation (DAP). The highest shoot formation rate (100%) was observed at a concentration of 300 mL L−1CW. The highest explant height addition (7.10 cm) was observed at a concentration of 200 mL L−1 CW. The highest number of leaves (5.80) was observed at a concentration of 1 mg L−1 BAP + 200 mL L−1 CW. The highest callus formation rate (50%) was observed at a concentration of 100 ml L−1CW and 300 ml L−1 CW. The highest number of roots (17) was observed in the control.

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