Intraspecifïc variation in gel electrophoresis patterns of soluble mycelial proteins of Phytophthora megasperma isolated from alfalfa

1986 ◽  
Vol 64 (2) ◽  
pp. 262-265 ◽  
Author(s):  
M. A. Faris ◽  
F. E. Sabo ◽  
Y. Cloutier
Keyword(s):  
Intraspecific Variation ◽  
Gel Electrophoresis ◽  
Protein Patterns ◽  
Phytophthora Megasperma ◽  
Highly Pathogenic ◽  
Electrophoretic Patterns ◽  
Group 2 ◽  
Cardinal Temperatures ◽  
Electrophoresis Technique ◽  
Group 1

The electrophoretic patterns of the buffer-soluble proteins of 26 isolates of Phytophthora megasperma f. sp. medicaginis from diseased alfalfa plants and soil were studied. On the basis of the protein patterns, the isolates were divided into two groups which correlated with cardinal temperatures for growth, pathogenicity, and oogonia size. Group 1 (seven isolates) has minimum growth at 5 °C, optimum at 25–30 °C, and maximum at 35 °C, and is a highly pathogenic pathotype with small oogonia. Group 2 (19 isolates) has minimum growth at less than 5 °C, optimum at 20 °C, and maximum at 30 °C, and is a less pathogenic pathotype with large oogonia. The electrophoresis technique was useful in identifying differences among the Phytophthora isolates.

The systematics of Phytophthora sojae and P. megasperma

1989 ◽  
Vol 67 (5) ◽  
pp. 1442-1447 ◽  
Author(s):  
M. A. Faris ◽  
F. E. Sabo ◽  
D. J. S. Barr ◽  
C. S. Lin
Keyword(s):  
Temperature Response ◽  
Phytophthora Sojae ◽  
Phytophthora Megasperma ◽  
Highly Pathogenic ◽  
Formae Speciales ◽  
Type Isolate ◽  
Cardinal Temperatures

Isolates of Phytophthora from alfalfa and soybean and the type isolate of Phytophthora megasperma Drechsler from hollyhock were compared morphologically, for temperature response, and pathogenically. Highly pathogenic, host-specific isolates from soybean and alfalfa, with relatively small oogonia, were identified as P. sojae Kaufmann and Gerdemann and divided into two formae speciales: f.sp. glycines and f.sp. medicaginis, respectively. Less pathogenic isolates from alfalfa with large oogonia were identified as P. megasperma and were compared favorably with Drechsler's original isolate and description. Isolates of P. sojae differed in their cardinal temperatures from those of P. megasperma. We consider P. sojae to be distinct from P. megasperma and propose that this taxon be reintroduced to the literature.

ELECTROPHORETIC PATTERNS OF EGG PROTEINS FROM SEVERAL INSECT TAXA

1969 ◽  
Vol 101 (12) ◽  
pp. 1256-1265 ◽  
Author(s):  
E. H. Salkeld
Keyword(s):  
Related Species ◽  
Gel Electrophoresis ◽  
Congeneric Species ◽  
Protein Patterns ◽  
Closely Related Species ◽  
Electrophoretic Patterns ◽  
Phylogenetic Studies ◽  
Egg Protein ◽  
Family Resemblances ◽  
Species Specific

AbstractEgg-protein patterns from 23 insect species representing seven orders were obtained by acrylamide gel electrophoresis. The patterns were species specific and highly reproducible. Those for congeneric species were very similar and family resemblances were apparent. The usefulness of egg-protein patterns in phylogenetic studies and in the analysis of closely related species was suggested.

Proteinases secreted by Fasciola hepatica: time course of the inhibitory effect of serum from experimentally infected rabbits demonstrated by gelatin-substrate polyacrylamide gel electrophoresis

1997 ◽  
Vol 71 (4) ◽  
pp. 333-338 ◽  
Author(s):  
L. Piacenza ◽  
D. Acosta ◽  
A. Dowd ◽  
S. McGonicle ◽  
J. Dalton ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Neutralizing Antibodies ◽  
Time Course ◽  
Fasciola Hepatica ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

AbstractFasciola hepatica secretes proteolytic enzymes to aid it to penetrate and migrate through the host tissues. Two of these proteinases have been purified and shown to be cathepsin L-like, and are termed, CL1 (27.5 kD) and CL2 (29 kD). The immunogenicity of these proteinases was investigated over the course of an experimental infection and following drug treatment. Four groups of rabbits were studied: group 1: orally infected with 50 metacercariae; group 2: infected and treated 8 weeks after infection; group 3: infected, treated at week 8 and reinfected at week 13 and group 4: non-infected control group. Sera were collected weekly from each group until week 20 postinfection. CL1 and CL2 were incubated with the different sera and then analysed by gelatin substrate polyacrylamide gel electrophoresis (GS-PAGE). Analysis of groups 1, 2 and 3 showed that CL1 and CL2 neutralizing antibodies appear at week 5 post-infection. In group 1, these remained throughout the 20 weeks of infection. In group 2, neutralizing antibodies disappeared at week 13, that is, 5 weeks after anti-Fasciola treatment. In group 3, CL1 and CL2 neutralizing antibodies disappeared at week 13 but reappeared by week 15, that is 2 weeks after reinfection. Pooled sera from group 4, showed no inhibitory capacity. ELISA results using CL1 and CL2 as antigen, correlate very well with the inhibitiory time course observed by GS-PAGE. These results suggest that purified cathepsin Ls are antigenic molecules recognized early in the infective process and capable of inducing a specific humoral response, strong enough to neutralize, at least partially, their enzymatic activity.

Vertical Plate Gel Electrophoresis for the Differentiation of Fish and Shellfish Species

1972 ◽  
Vol 55 (3) ◽  
pp. 464-466
Author(s):  
R J Coduri ◽  
A G Rand
Keyword(s):  
Gel Electrophoresis ◽  
Vertical Plate ◽  
Polyacrylamide Gel Electrophoresis ◽  
Direct Analysis ◽  
Buffer System ◽  
Electrophoretic Patterns ◽  
Extraction Buffer ◽  
Frozen Fish ◽  
Fish And Shellfish ◽  
Electrophoresis Technique

Abstract The vertical plate polyacrylamide gel electrophoresis technique described for differentiation of meat was applied to fresh and frozen fish and shellfish species. A single 7% Cyanogum gel containing mercaptoethanol, in conjunction with a tris-chloride, tris-glycine discontinuous buffer system, produced satisfactory resolution of fish and shellfish species extracts. The electrophoretic patterns were obtained by direct analysis of the proteins extracted with a tris-chloride-10% glucose extraction buffer at pH 6.7. This technique can also be used to detect substitution of one fish or shellfish species for another.

ASSOCIATION BETWEEN GLIADIN ELECTROPHORETIC BANDS AND QUALITY IN COMMON WHEAT

1982 ◽  
Vol 62 (4) ◽  
pp. 913-918 ◽  
Author(s):  
N. E. POGNA ◽  
G. BOGGINI ◽  
M. CORBELLINI ◽  
M. CATTANEO ◽  
A. DAL BELIN PERUFFO
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Triticum Aestivum L ◽  
Loaf Volume ◽  
Wheat Cultivars ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Twenty common wheat (Triticum aestivum L.) cultivars were classified into two groups based on the presence or absence in their electrophoregrams of two gliadin bands separated by polyacrylamide gel electrophoresis. The two groups were compared for quality and agronomic characteristics evaluated during 6 yr of testing. The group 1 cultivars (band 40 present, 43.5 absent) had weaker gluten properties and lower loaf volume than cultivars in group 2 (band 40 absent, 43.5 present). No significant difference between the groups was found for protein content or grain yield. The present data are discussed in relation to similar results obtained by other authors for durum wheat cultivars.

In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection?

VASA ◽  
2020 ◽  
Vol 49 (4) ◽  
pp. 281-284
Author(s):  
Atıf Yolgosteren ◽  
Gencehan Kumtepe ◽  
Melda Payaslioglu ◽  
Cuneyt Ozakin
Keyword(s):  
Vascular Graft ◽  
Graft Infection ◽  
Vascular Graft Infection ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Prosthetic Vascular Graft Infection ◽  
Group 1

Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.

The influence of continuous local wound infusion on postoperative pain in patients undergoing transfemoral amputation

VASA ◽  
2015 ◽  
Vol 44 (5) ◽  
pp. 381-386 ◽  
Author(s):  
Christian Uhl ◽  
Thomas Betz ◽  
Andrea Rupp ◽  
Markus Steinbauer ◽  
Ingolf Töpel
Keyword(s):  
Pain Management ◽  
Pilot Study ◽  
Local Anaesthetic ◽  
Phantom Pain ◽  
Transfemoral Amputation ◽  
Postoperative Wound ◽  
Stump Pain ◽  
Management Protocol ◽  
Group 2 ◽  
Group 1

Abstract. Summary: Background: This pilot study was set up to examine the effects of a continuous postoperative wound infusion system with a local anaesthetic on perioperative pain and the consumption of analgesics. Patients and methods: We included 42 patients in this prospective observational pilot study. Patients were divided into two groups. One group was treated in accordance with the WHO standard pain management protocol and in addition to that received a continuous local wound infusion treatment (Group 1). Group 2 was treated with analgesics in accordance with the WHO standard pain management protocol, exclusively. Results: The study demonstrated a significantly reduced postoperative VAS score for stump pain in Group 1 for the first 5 days. Furthermore, the intake of opiates was significantly reduced in Group 1 (day 1, Group 1: 42.1 vs. Group 2: 73.5, p = 0.010; day 2, Group 1: 27.7 vs. Group 2: 52.5, p = 0.012; day 3, Group 1: 23.9 vs. Group 2: 53.5, p = 0.002; day 4, Group 1: 15.7 vs. Group 2: 48.3, p = 0.003; day 5, Group 1 13.3 vs. Group 2: 49.9, p = 0.001). There were no significant differences between the two groups, neither in phantom pain intensity at discharge nor postoperative complications and death. Conclusions: Continuous postoperative wound infusion with a local anaesthetic in combination with a standard pain management protocol can reduce both stump pain and opiate intake in patients who have undergone transfemoral amputation. Phantom pain was not significantly affected.

Abnormal Haemostasis and Blood Viscosity in Malignant Hypertension

1984 ◽  
Vol 52 (03) ◽  
pp. 253-255 ◽  
Author(s):  
C Isles ◽  
G D O Lowe ◽  
B M Rankin ◽  
C D Forbes ◽  
N Lucie ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Blood Viscosity ◽  
Renal Damage ◽  
Antithrombin Iii ◽  
Plasma Viscosity ◽  
Malignant Hypertension ◽  
Fibrin Deposition ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

SummaryWe have previously shown abnormalities of haemostasis suggestive of intravascular coagulation in patients with malignant hypertension, a condition associated with retinopathy and renal fibrin deposition. To determine whether such abnormalities are specific to malignant hypertension, we have measured several haemostatic and haemorheological variables in 18 patients with malignant hypertension (Group 1), 18 matched healthy controls (Group 2), and 18 patients with non-malignant hypertension (Group 3) matched for renal pathology, blood pressure and serum creatinine with Group 1. Both Groups 1 and 3 had increased mean levels of fibrinogen, factor VIIIc, beta-thrombo- globulin, plasma viscosity and blood viscosity (corrected for haematocrit); and decreased mean levels of haematocrit, antithrombin III and platelet count. Mean levels of fast antiplasmin and alpha2-macroglobulin were elevated in Group 1 but not in Group 3. We conclude that most blood abnormalities are not specific to malignant hypertension; are also present in patients with non-malignant hypertension who have similar levels of blood pressure and renal damage; and might result from renal damage as well as promoting further renal damage by enhancing fibrin deposition. However increased levels of fibrinolytic inhibitors in malignant hypertension merit further investigation in relation to removal of renal fibrin.

Total Knee Arthroplasty after Previous Ipsilateral Hip Arthroplasty Showed Lower Clinical Outcomes and Higher Leg Length Discrepancy Perception

2020 ◽  
Author(s):  
Han-Jun Lee ◽  
Seong Hwan Kim ◽  
Nicolas Pujol ◽  
Yong-Beom Park
Keyword(s):  
Clinical Outcomes ◽  
Hip Arthroplasty ◽  
Leg Length ◽  
Function Score ◽  
Patient’S Satisfaction ◽  
Length Discrepancy ◽  
Total Knee ◽  
Group 2 ◽  
Ks Function ◽  
Group 1

AbstractThe purpose of this study is to compare perception of leg length discrepancy (LLD) and clinical results of total knee arthroplasties (TKA) in patients with or without previous ipsilateral hip arthroplasty. Between 2008 and 2015, navigation-assisted TKA was performed in 43 patients with previous hip arthroplasty after hip fracture. After 1:3 propensity score matching was performed, 108 patients of primary navigation-assisted TKA (group 1) and 36 patients with hip arthroplasty (group 2) were included. Knee Society (KS) scores, Western Ontario and McMaster Universities Index (WOMAC) scores, and patients' satisfaction including perception of LLD were evaluated. Radiographic evaluation included mechanical axis, component position, and LLD. Logistic regression analysis was performed to find the factors that affect the clinical outcomes. No significant differences in radiologic and clinical evaluations, except for KS function score, patient's satisfaction and LLD (p< 0.001), were detected between the groups. LLD and its perception were significantly higher in group 2 (1.8 ± 3.4 mm in group 1 and 9.7 ± 4.1 mm in group 2, p = 0.000). Risk factors for the low KS function score were found as LLD (odds ratio [OR]: 1.403, p = 0.008) and previous hip arthroplasty itself (OR: 15.755, p = 0.002), but much higher OR was found in previous hip arthroplasty. Although the outcomes of TKA in patients with ipsilateral hip arthroplasty are comparable to those of primary TKA, LLD was high and patient's satisfaction and functional outcomes were low in patients with previous ipsilateral hip arthroplasty. Care should be taken when considering TKA in patients with previous hip arthroplasty. This is a Level III, case control study.

Clinical and Radiological Changes after use of Alpha-1 Blocker Therapy in Patients of Posterior Urethral Valve with Bladder Neck Hypertrophy

JMS SKIMS ◽  
2009 ◽  
Vol 12 (1) ◽  
pp. 3-7
Author(s):  
Aejaz A Baba ◽  
Bajpai Minu
Keyword(s):  
Bladder Neck ◽  
Posterior Urethral Valve ◽  
Posterior Urethral Valves ◽  
Blocker Therapy ◽  
Urethral Valve ◽  
Novel Method ◽  
Radiological Changes ◽  
Group 2 ◽  
Mean Time ◽  
Group 1

Background: We evaluated the effects of alpha-1 blocker therapy on clinical and radiological abnormalities in patients of posterior urethral valve with bladder neck hypertrophy. Materials and Methods: A total of 74 patients with posterior urethral valves were seen at our department between 2003 and 2007. Out of these 24 had radiological evidence of bladder neck hypertrophy. Those patients with bladder neck hypertrophy who were seen before June 2006 and did not receive alpha-1 blocker (prazocin) therapy after valve ablation were assigned to group 1 (n=10). Group 2 consisted of 14 age matched patients with bladder neck hypertrophy and comparable prognostic factors who received alpha-1 blocker therapy after valve ablation (n=14). Micturating cystourethrography (MCU) was done periodically and a novel method used to calculate bladder neck hypertrophy. Trends in symptoms and radiological changes were evaluated throughout follow-up. Results: Mean patient age at presentation was 3.02±2.68 years in group 1 and 3.12±3.4 years in group 2. Bladder neck hypertrophy decreased from 2.3±1.0 to 2.0±0.5 in a mean time of 52.0 (34-52) weeks in group 1 where as it decreased from 2.2±0.5 to 1.6±0.3 in a mean time of 32.0 (22-52) weeks. Symptomatically patients in group 2 who received alpha-1 blocker therapy after valve ablation were better and had quick resolution of bladder neck hypertrophy. Conclusions: Use of alpha-1 blocker therapy in patients of posterior urethral valve with bladder neck hypertrophy helps in quick resolution of bladder neck hypertrophy. J Med Sci 2009;12(1):3-7.

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