Stomatal and anatomical leaf characteristics of 10 Populus clones

1984 ◽  
Vol 62 (3) ◽  
pp. 513-518 ◽  
Author(s):  
R. Ceulemans ◽  
I. Impens ◽  
V. Steenackers
Keyword(s):  
Leaf Surface ◽  
Principal Component ◽  
Stem Volume ◽  
Volume Data ◽  
Stomatal Frequency ◽  
Anatomical Characteristics ◽  
Abaxial Leaf Surface ◽  
Place Of Origin ◽  
Anatomical Characters ◽  
Stomatal Length

Stomatal characteristics (frequency and length of stomata on adaxial and abaxial leaf surface) and anatomical characteristics (thickness of epidermal, palisade, and spongy layers) of field-grown plants of Populus clones, six of American, two of European, and two of Euramerican origin, were studied. All clones except one were amphistomatous and all clones had most stomata on the abaxial leaf surface. In all clones the spongy parenchyma layer was thicker than the palisade layer. Stomatal and anatomical characteristics varied significantly among clones. Both cluster analysis and principal-component analysis, utilizing mean clonal values of stomatal and anatomical characters as independent variables, indicated that these characteristics were related to place of origin and to parentage. Different groupings of the clones were observed on the basis of abaxial stomatal length and total stomatal frequency. Abaxial and mean stomatal length were significantly correlated with yield (represented by stem volume data), but relationships between other characteristics and yield were not significant.

scholarly journals Leaf anatomy of Qualea parviflora (Vochysiaceae) in three phytophysiognomies of the Mato Grosso State, Brazil

2016 ◽  
Vol 46 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Ana Paula Ramos ARIANO ◽  
Ivone Vieira da SILVA
Keyword(s):  
Cell Walls ◽  
Leaf Surface ◽  
Leaf Traits ◽  
Vascular Bundles ◽  
Intercellular Spaces ◽  
Dissociation Process ◽  
Mato Grosso ◽  
Epidermal Tissue ◽  
Abaxial Leaf Surface ◽  
Anatomical Characters

ABSTRACT Leaves have a variety of morphological and anatomical characters mainly influenced by climatic, edaphic and biotic factors. The aim of this study was to describe the anatomical leaf traits of Qualea parviflora from three phytophysiognomies. The studied phytophysiognomies were Amazon Savannah on rocky outcrops (ASR), Transition Rupestrian Cerrado (TRC), and Cerradão (CDA). Freehand sections of the leaf blade were made and stained with 0.5% astra blue and with basic fuchsin. From the adaxial and abaxial leaf surface, freehand paradermal sections were made for epidermis analysis. The Jeffrey´s method, with modifications, was used in the epidermis dissociation process. The samples from the TRC phytophysiognomy had relatively smaller ordinary epidermal cells, higher abundance of trichomes, and mesophyll with few intercellular spaces, in comparison to the other phytophysiognomies. The leaves from the ASR phytophysiognomy had higher stomatal index (SI = 21.02), and five to six layers of sclerenchyma surrounding the midrib vascular bundle. The secondary vascular bundles had thicker cell walls and the bundle sheath extended up to the epidermal tissue of both leaf sides. Leaves from the CDA phytophysiognomy had mesomorphic environmental traits, such as a thinner cuticle. It is concluded that trees from ASR and TRC phytophysiognomies have xeromorphic traits following the environmental conditions where they occur.

Characterization of stomatal development in Dianthus chinensis

1988 ◽  
Vol 66 (1) ◽  
pp. 142-149 ◽  
Author(s):  
Thalia Pappas ◽  
Patricia McManus ◽  
Peter Vanderveer ◽  
Judith Croxdale
Keyword(s):  
Leaf Blade ◽  
Leaf Surface ◽  
Guard Cells ◽  
Stomatal Development ◽  
Stomatal Frequency ◽  
Stomatal Complex ◽  
Adaxial Surface ◽  
Abaxial Leaf Surface ◽  
Area Basis

Stomatal differentiation in Dianthus chinensis leaves was characterized structurally and functionally using plants of the seventh plastochron. Development of stomata was followed using scanning electron microscopy. The maturity of the stomatal complex, the existence of an open stomatal pore, and the localization of K+ within guard cells were used as indicators of stomatal function. The results showed that stomatal development spanned a 10- to 12-day period, which began on the fourth youngest pair of leaves and was completed in the middle of the blade of the sixth youngest leaf pair where stomatal function was acquired. Stomatal frequency on an area basis decreased from the base to the tip of the leaf blade and from the abaxial to the adaxial surface of the leaf. There was a 10-fold decline in the frequency of stomata during leaf maturation; frequency on the abaxial leaf surface was about twice that on the adaxial surface. Dimensions of the antechamber aperture changed during development but at maturity exhibited a range of lengths and widths that was independent of stomatal location on the leaf. The localization of K+ in guard cells occurred only in the later stages of differentiation and was absent in stomata of senescing leaves.

scholarly journals Phenetic analysis of Curcuma spp. in Yogyakarta, Indonesia based on morphological and anatomical characters

2019 ◽  
Vol 20 (8) ◽  
Author(s):  
Mulya Sungkawati ◽  
L. HIDAYATI ◽  
B.S. DARYONO ◽  
Purnomo Purnomo
Keyword(s):  
Economic Value ◽  
Similarity Index ◽  
Morphological Characteristics ◽  
Principal Component ◽  
Phenetic Analysis ◽  
Anatomical Characteristics ◽  
Morphological Variations ◽  
Phenetic Relationships ◽  
Anatomical Characters ◽  
Group B

Abstract. Sungkawati M, Hidayati L, Daryono BS, Purnomo. 2019. Phenetic analysis of Curcuma spp. in Yogyakarta, Indonesia based on morphological and anatomical characters. Biodiversitas 20: 2340-2347. Curcuma spp., also known as ginger (Zingiberaceae), has economic value in traditional medicine. However, its many morphological variations cause difficulties in identification and classification. Therefore, observation of its morphological and anatomical characteristics, and of the phenetic relationships between Curcuma species, is important. This research aims to determine the specific characteristics of Curcuma spp. and to examine its phenetic relationships based on morphological and anatomical characteristics. The research was conducted in July 2018-February 2019 in the Bantul, Gunungkidul, Sleman and Karanganyar regions. Observation of the anatomical characteristics was conducted on the leaves and rhizomes and the data analyzed according to descriptive and quantitative/numerical methods. Clustering analysis with the Gower General Similarity Coefficient and Principal Component Analysis (PCA) was performed to determine the role of each character in groupings. The results of the research found seven species from 23 OTUs observed (C. aeruginosa, C. domestica, C. manga and C. xanthorrhiza, each with four OTUs; C. soloensis and C. zedoaria, with three OTUs each; and C. heyneana with one OTU) and showed that specific morphological characteristics were found in the flesh color rhizome, pseudostem color and midrib color. Specific anatomical characteristics were evident in the secretion cell color and the presence of trichomes on the leaves and rhizomes. The dendrogram shows a 0.70 phenon line consisting of two groups, group A (C. soloensis and C. domestica) fused in a 0.760 similarity index, and group B (C. aeruginosa, C. mangga, C. heyneana, C. soloensis, C. xanthorrhiza and C. zedoaria) fused in a 0.654 similarity index, which means that C. soloensis and C. domestica have a close phenetic relationship. The 0.80 phenon line consisted of five groups: C. domestica, C. soloensis, C. xanthorrhiza, C. zedoaria-C. mangga-C. heyneana and C. aeruginosa.

scholarly journals Carpinus tibetana (Betulaceae), a new species from southeast Tibet, China

PhytoKeys ◽  
2018 ◽  
Vol 98 ◽  
pp. 1-13 ◽  
Author(s):  
Zhiqiang Lu ◽  
Ying Li ◽  
Xiaoyue Yang ◽  
Jianquan Liu
Keyword(s):  
New Species ◽  
Leaf Surface ◽  
Principal Component ◽  
Geographic Isolation ◽  
Morphometric Characters ◽  
Abaxial Leaf Surface ◽  
Sequence Variations ◽  
Genetic Clusters ◽  
Southeast Tibet ◽  
A New Species

A new species Carpinustibetana Z. Qiang Lu & J. Quan Liu from southeast Tibet is described and illustrated. The specimens of this new species were previously identified and placed under C.monbeigiana Hand.-Mazz. or C.mollicoma Hu. However, the specimens from southeast Tibet differ from those of C.monbeigiana from other regions with more lateral veins (19–24 vs 14–18) on each side of the midvein and dense pubescence on the abaxial leaf surface, while from those of C.mollicoma from other regions differ by nutlet with dense resinous glands and glabrous or sparsely villous at apex. Principal Component Analyses based on morphometric characters recognise the Tibetan populations as a separate group. Nuclear ribosomal ITS sequence variations show stable and distinct genetic divergences between the Tibetan populations and C.monbeigiana or C.mollicoma by two or three fixed nucleotide mutations. Phylogenetic analysis also identified three respective genetic clusters and the C.mollicoma cluster diverged early. In addition, the Tibetan populations show a disjunct geographic isolation from the other two species. Therefore, C.tibetana, based on the Tibetan populations, is here erected as a new species, distinctly different from C.monbeigiana and C.mollicoma.

Picking sides: feeding on the abaxial leaf surface is costly for caterpillars

Planta ◽  
2021 ◽  
Vol 253 (4) ◽  
Author(s):  
Sakshi Watts ◽  
Rupesh Kariyat
Keyword(s):  
Leaf Surface ◽  
Abaxial Leaf Surface

scholarly journals Selection of Eucalyptus camaldulensis Families for Sustainable Pulpwood Production by Means of Anatomical Characteristics

Forests ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 31
Author(s):  
Ikumi Nezu ◽  
Futoshi Ishiguri ◽  
Haruna Aiso ◽  
Sapit Diloksumpun ◽  
Jyunichi Ohshima ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Fibre Diameter ◽  
Eucalyptus Camaldulensis ◽  
Principal Component ◽  
Wood Properties ◽  
Component Analysis ◽  
Pulp And Paper ◽  
Anatomical Characteristics ◽  
Diameter Fibre ◽  
Sib Families

Sustainable pulpwood production from fast-growing tree plantations is needed for pulp and paper industries. To increase the pulpwood production efficiency, the anatomical characteristics and derived-wood properties of 75 trees from 15 half-sib families of Eucalyptus camaldulensis Dehnh. planted in Thailand were investigated, and then the family was classified by suitability of wood as raw material for pulp and paper products using principal component analysis and clustering. The mean values of vessel diameter, vessel frequency, fibre diameter, fibre lumen diameter, and fibre wall thickness at 2 cm from the cambium were 128 µm, 16 no./mm2, 11.1 µm, 7.1 µm, and 1.88 µm, respectively. In addition, the Runkel ratio, Luce’s shape factor, flexibility coefficient, slenderness ratio, solids factor, and wall coverage ratio (i.e., derived-wood properties) were 0.53, 0.42, 0.64, 85.3, 68 × 103 µm3, and 0.34, respectively. Significant differences in fibre diameter, fibre lumen diameter, and Runkel ratio were found among families. Although significant differences among families were not found for other anatomical characteristics and derived-wood properties, the p-values obtained by an analysis of variance test ranged from 0.050 to 0.088. Based on the results of a principal component analysis and cluster analysis, 15 families were classified into four clusters with different expected pulp and paper characteristics. The suitability of wood from E. camaldulensis half-sib families for pulp and paper can be evaluated by principal component analysis using anatomical characteristics and physical properties as variables. Based on the results, desirable pulp and paper quality may be obtained through the selection of families from this species.

Cryptic speciation in the herbaceous bamboo genus Piresia (Poaceae, Olyreae)

2019 ◽  
Author(s):  
Maria L Silveira de Carvalho ◽  
Izabela S D de Jesus ◽  
Rilquer M da Silva ◽  
Kelly R B Leite ◽  
Alessandra S Schnadelbach ◽  
...  
Keyword(s):  
New Species ◽  
Atlantic Forest ◽  
Leaf Surface ◽  
Conservation Status ◽  
Integrative Approach ◽  
Niche Modelling ◽  
The North ◽  
Abaxial Leaf Surface ◽  
Herbaceous Bamboos ◽  
North Eastern

Abstract Piresia, a small genus of herbaceous bamboos, has a geographical disjunction between the Caribbean and northern/western South America and the north-eastern Atlantic Forest in Brazil. Piresia leptophylla is reported from western Amazonia (WA) and the north-eastern Atlantic Forest (NAF), but its occurrence in western Amazonia is questionable. Using an integrative approach, we combined traditional morphological analysis, anatomy and niche modelling. The results revealed few macromorphological differences between WA and NAF specimens (only plant height, leaf length, lodicule dimensions, shape and position), contrasting with consistent differences in leaf anatomy (macrohairs and cruciform silica bodies in the costal zone of the adaxial/abaxial leaf surfaces, crenate silica bodies on the abaxial leaf surface, lack of panicoid hairs on the abaxial leaf surface, bicellular microhairs and lobed papillae over the abaxial leaf surface, and sparse but elongated fusoid cells in the mesophyll of WA specimens) and in niche patterns. The anatomical/micromorphological characters suggest environmental adaptations to the Amazonian and ‘restinga’ forests, respectively. We therefore propose the segregation of the WA populations into a new species, Piresia tenella sp. nov. We provide a formal description, photographs, a line illustration, a distribution map and discussion of the conservation status for the new species.

scholarly journals Adjuvant Effects on Imazethapyr, 2,4-D and Picloram Absorption by Leafy Spurge (Euphorbia esula)

Weed Science ◽  
1996 ◽  
Vol 44 (3) ◽  
pp. 469-475 ◽  
Author(s):  
W. Mack Thompson ◽  
Scott J. Nissen ◽  
Robert A. Masters
Keyword(s):  
Ammonium Nitrate ◽  
Ammonium Sulfate ◽  
Seed Oil ◽  
Leaf Surface ◽  
Leafy Spurge ◽  
Cuticular Waxes ◽  
Adaxial Leaf Surface ◽  
Abaxial Leaf Surface ◽  
Urea Ammonium Nitrate ◽  
Adjuvant Effects

Laboratory experiments were conducted to identify adjuvants that improve absorption of imazethapyr, 2,4-D amine, and picloram by leafy spurge. Adjuvants (0.25% v/v) included crop oil concentrate (COC), methylated seed oil (MSO), nonionic surfactant (NIS), organosilicones (Silwet L-77®, Sylgard® 309, Silwet® 408), 3:1 mixtures of acetylinic diol ethoxylates (ADE40, ADE65, ADE85) with Silwet L-77, ammonium sulfate (2.5 kg ha−1), and 28% urea ammonium nitrate (UAN, 2.5% v/v). Adjuvants were combined with14C-herbicide and commercially formulated herbicide product. Leaves were harvested 2 DAT, rinsed with 10% aqueous methanol to remove surface deposits of herbicide, and dipped in 9:1 hexane:acetone to solubilize cuticular waxes. Imazethapyr absorption increased by 38 to 68% when UAN was combined with COC, NIS, or MSO. Total absorption of imazethapyr plus COC, MSO, or NIS exceeded 86% 2 DAT when UAN was added. Urea ammonium nitrate reduced the amount of imazethapyr associated with the cuticular wax by 2.0%. Imazethapyr absorption was similar on both the abaxial and adaxial leaf surface when UAN was not added; however, 12% more imazethapyr was absorbed from the abaxial leaf surface than from the adaxial leaf surface when UAN was combined with Sylgard 309. Uptake of 2,4-D ranged from 54 to 78% and was greatest with Silwet 408 and 3:1 mixture of ADE40: Silwet L-77. Picloram absorption ranged from 3 to 19%. Buffering picloram treatment solutions to pH 7 and including 2.5 kg ha-1ammonium sulfate increased picloram absorption to 37%.

scholarly journals Nutrient Release Pattern and Greenhouse-Grown Swiss Chard Response to Biochar Inoculated with Vermicast

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Lord Abbey ◽  
Jinsheng Cai ◽  
Lokanadha R. Gunupuru ◽  
Mercy Ijenyo ◽  
Ebenezer O. Esan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Response ◽  
Leaf Surface ◽  
Nutrient Release ◽  
Principal Component ◽  
Fresh Weight ◽  
Total Leaf Area ◽  
Total Leaf ◽  
Swiss Chard ◽  
Leaf Surface Area

A study was performed to assess nutrient release from biochar inoculated with solid vermicast (SVB), vermicast tea (VTB), deionized water (DWB), uninoculated biochar (Bioc), and Promix-BX (Pro-BX). The growth response of Swiss chard (Beta vulgaris subsp. vulgaris) cv. Rhubarb chard was also assessed. Comparatively, nutrients were released slowly from treatments SVB and VTB compared to the other treatments. The rate of nutrient release determined by total dissolved solids and electric conductivity from the Pro-BX was the highest. The trend for the plant growth components, total leaf surface area and leaf fresh weight at first harvest, was Pro-BX > Bioc > DWB = SVB > VTB. The only treatment that increased total leaf area and leaf fresh weight at the second harvest by approximately 1.02- and 1.88-fold was VTB. Leaf fresh weight was significantly reduced by approximately 0.33-fold for DWB, 0.28-fold for Bioc, and 0.70-fold for Pro-BX but was not altered by SVB at the second harvest as compared to the first harvest. A 2-dimensional principal component analysis (PCA) biplot confirmed that treatment Pro-BX increased plant growth components at the first harvest only. The locations of SVB and VTB on the PCA biplot confirmed their efficacies, which led to increases in the plant growth components at the second harvest. Overall, the VTB adsorbed more nutrients onto its surface that were slowly released to enhance the Swiss chard cv. Rhubarb chard plant growth at the second harvest. Further studies should consider microbial activities.

New and noteworthy Melastomataceae from the Yanachaga-Chemillén National Park and surrounding areas in Oxapampa, Pasco, Peru

Phytotaxa ◽  
2018 ◽  
Vol 374 (3) ◽  
pp. 185 ◽  
Author(s):  
FABIÁN A. MICHELANGELI ◽  
RENATO GOLDENBERG
Keyword(s):  
National Park ◽  
Leaf Surface ◽  
Nodal Line ◽  
Abaxial Surface ◽  
North East ◽  
The North ◽  
The Andes ◽  
Abaxial Leaf Surface ◽  
Surrounding Areas ◽  
Sclerophyllous Forests

We describe six new species of Melastomataceae from the Yanachaga-Chemillén National Park and surrounding areas from the Department of Pasco, Province of Oxapampa in Central Peru. Macrocentrum andinum is the first species of the genus described from the Andes, found along creeks at 400–500 m elev. and characterized by its anysophyllous leaves, pubescent stems and four-merous flowers. Meriania rubriflora is found in forests above 2200 m elev. and it is characterized by stem nodes with stipular flaps, leaves with an acute base and four merous, deep red flowers. Miconia palcazuana is found along rivers and streams at 300–400 m on the eastern flank of the park, and it can be distinguished by its flowers with pink anthers with glands on the connective and narrowly oblanceolate to elliptic-lanceolate leaves. Miconia yanachagaensis grows in the dwarf-sclerophyllous forests at the top of ridges and grasslands over 2800 m elev. and it is characterized by its long dendritic-pedicellate trichomes on the abaxial leaf surface, the stems flattened to terete and the presence of a conspicuous annular nodal line. Triolena rojasae is found growing on rocks along the Palcazú River and its tributaries, and it is characterized by its lanceolate-crenate leaves. Triolena vasquezii grows on the northern end of the Huancabamba canyon and the North East portion of the park and can be distinguished by its pustulate leaves with purple abaxial surface and anthers with two ventral appendages. We also present the first report of the genus Wurdastom for Peru.

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