Influence of abscisic acid on the derepression of the acid phosphatases in Ipomoea sp. cultured in vitro

1983 ◽  
Vol 61 (9) ◽  
pp. 2343-2348
Author(s):  
M. W. Zink
Keyword(s):  
Abscisic Acid ◽  
Culture Medium ◽  
Soluble Fraction ◽  
Morning Glory ◽  
Acid Phosphatases ◽  
Developmental Patterns ◽  
Phosphate Deprivation ◽  
Mineral Stress ◽  
High Phosphate

The effect of abscisic acid on the levels and the developmental patterns of the two acid phosphatases in the culture medium, in the soluble fraction, and in the particulate fraction of Ipomoea sp. (morning glory) cultured in vitro depends upon the phosphate status of the cells. Under conditions of mineral stress or phosphate deprivation the enzymes are derepressed and this derepression is suppressed by abscisic acid. No inhibition of the synthesis of the phosphatases by the hormone occurs when the cells are grown under conditions of high phosphate. The significance of the abscisic effect on the derepression of the acid phosphatases is discussed.

Acid phosphatases of Ipomoea sp. cultured in vitro. 1. Influence of pH and inorganic phosphate on the formation of phosphatases

1979 ◽  
Vol 57 (7) ◽  
pp. 739-753 ◽  
Author(s):  
M. W. Zink ◽  
I. A. Veliky
Keyword(s):  
Inorganic Phosphate ◽  
Culture Medium ◽  
Soluble Fraction ◽  
Morning Glory ◽  
Acid Phosphatases ◽  
Developmental Patterns ◽  
Low Concentrations ◽  
Shake Flasks ◽  
Influence Of Ph

The levels and the developmental patterns of the two acid phosphatases in Ipomoea sp. (morning glory) were influenced by the pH of the medium and whether the cultures were grown in fermentors or shake flasks. The two enzymes, which appeared in the culture medium, in the soluble fraction, and in the particulate fraction, were derepressed when suspension cultures were grown in a medium containing low concentrations of inorganic phosphate. The addition of up to 4 μmol of phosphate per millilitre to cells grown for 4 days on low phosphate did not repress the synthesis of the enzymes. However, the addition of excess phosphate resulted in a temporary cessation of phosphatase synthesis. Inorganic phosphate appeared to be only one of several factors controlling the levels of the enzymes.

Acid phosphatases of Ipomoea sp. cultured in vitro. 2. Influence of gibberellic acid on the formation of phosphatases

1980 ◽  
Vol 58 (20) ◽  
pp. 2171-2180 ◽  
Author(s):  
M.W. Zink
Keyword(s):  
Gibberellic Acid ◽  
Specific Activity ◽  
Soluble Fraction ◽  
Morning Glory ◽  
Original Strain ◽  
Acid Phosphatases ◽  
Developmental Patterns ◽  
Stages Of Growth ◽  
Repressive Effect

The levels and developmental patterns of the two acid phosphatases in the two strains of Ipomoea sp. (morning glory) grown in vitro are influenced differently by gibberellic acid (GA3). In the strain that requires a number of exogenously added hormones for growth (original strain), GA3 appears to show little effect on the specific activity of the phosphatases during the initial 3 days of growth but represses their levels in the growth medium, soluble and particulate fractions upon further growth. The repressive effect of both inorganic phosphate and GA3 on the enzymes appears to be additive. In the strain that does not require exogenously added hormones for growth (NH strain), the level of enzymes in the soluble fraction increases in the presence of GA3 during the early stages of growth and then decreases with culture age. GA3 also elevates the level of the enzymes in the particulate fraction over that in the controls. This elevation increases as phosphate level in the medium is increased. However, GA3 greatly decreases secretion, or leaching of the enzymes from the cells.

scholarly journals Rhizobacteria Inoculation Effects on Phytohormone Status of Potato Microclones Cultivated In Vitro under Osmotic Stress

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1231
Author(s):  
Tatiana N. Arkhipova ◽  
Nina V. Evseeva ◽  
Oksana V. Tkachenko ◽  
Gennady L. Burygin ◽  
Lidiya B. Vysotskaya ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Polyethylene Glycol ◽  
Azospirillum Brasilense ◽  
Culture Medium ◽  
Crop Productivity ◽  
Urgent Problem ◽  
Hormonal Balance ◽  
Peg Treatment ◽  
Mass Accumulation

Water deficits inhibit plant growth and decrease crop productivity. Remedies are needed to counter this increasingly urgent problem in practical farming. One possible approach is to utilize rhizobacteria known to increase plant resistance to abiotic and other stresses. We therefore studied the effects of inoculating the culture medium of potato microplants grown in vitro with Azospirillum brasilense Sp245 or Ochrobactrum cytisi IPA7.2. Growth and hormone content of the plants were evaluated under stress-free conditions and under a water deficit imposed with polyethylene glycol (PEG 6000). Inoculation with either bacterium promoted the growth in terms of leaf mass accumulation. The effects were associated with increased concentrations of auxin and cytokinin hormones in the leaves and stems and with suppression of an increase in the leaf abscisic acid that PEG treatment otherwise promoted in the potato microplants. O. cytisi IPA7.2 had a greater growth-stimulating effect than A. brasilense Sp245 on stressed plants, while A. brasilense Sp245 was more effective in unstressed plants. The effects were likely to be the result of changes to the plant’s hormonal balance brought about by the bacteria.

scholarly journals The effect of sucrose concentration in the culture medium on the formation of abscisic acid and the activity of the photosynthetic apparatus of grape plants in vitro

2020 ◽  
Author(s):  
M. A. Sundyreva ◽  
A. N. Rebrov ◽  
A. E. Mishko ◽  
E. O. Lutsky
Keyword(s):  
Gene Expression ◽  
Abscisic Acid ◽  
Quantum Yield ◽  
Culture Medium ◽  
Sucrose Concentration ◽  
Photosynthetic Apparatus ◽  
Sucrose Content ◽  
Growth Processes ◽  
Expression Of Genes

An increase in sucrose in the medium increased the content of pigments, gene expression of the photosynthetic apparatus, growth processes, and H2O2, but decreased the quantum yield of photosynthesis. With a change in the sucrose content in the medium, the expression of ABA1 increased most intensively. 30 g/l of sucrose in the medium inhibited the expression of genes involved in the formation of ABA.

Effet des noeuds sur la direction de croissance des bourgeons axillaires des stolons de Stachys silvatica

1980 ◽  
Vol 58 (4) ◽  
pp. 466-470 ◽  
Author(s):  
E. Pfirsch ◽  
Th. Makosso
Keyword(s):  
Abscisic Acid ◽  
Culture Medium ◽  
Axillary Buds ◽  
Single Node ◽  
Horizontal Growth

The plagiogeotropic growth of axillary buds of the epigeous autonomous stolon of Stachys silvatica is governed by multiple correlations inside this stolon. The horizontal growth of axillary buds on single node cultures depends on the action of a stolonisation substance, and on the presence of leaves on this node; leaves can be replaced with abscisic acid. The growth of the isolated bud, cultured in vitro, is always orthotropic, even though a stolonisation substance and abscisic acid are added to the culture medium. It thus appears that the node is the decisive element for horizontal growth.

Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: Modulation by calcium

1991 ◽  
Vol 125 (3) ◽  
pp. 280-285 ◽  
Author(s):  
J. Alan Talbot ◽  
Ann Lambert ◽  
Robert Mitchell ◽  
Marek Grabinski ◽  
David C. Anderson ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Culture Medium ◽  
Follicle Stimulating Hormone ◽  
Dibutyryl Camp ◽  
Immature Rat ◽  
Estradiol Secretion ◽  
Old Rats ◽  
Stimulating Hormone

Abstract We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/1) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091±322 to 1480±84 pmol/l (p<0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360±45 to 1242±133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 μmol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 μmol/l) and A23187 (2 μmol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by >80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 μmol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 μmol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.

Pulsatile GnRH secretion from primary cultures of sheep olfactory placode explants

Reproduction ◽  
2000 ◽  
pp. 391-396 ◽  
Author(s):  
AH Duittoz ◽  
M Batailler
Keyword(s):  
Culture Medium ◽  
Primary Cultures ◽  
Pulsatile Secretion ◽  
Olfactory Placode ◽  
Gnrh Secretion ◽  
Individual Culture ◽  
Pulsatile Gnrh

The aim of this study was to investigate the development of pulsatile GnRH secretion by GnRH neurones in primary cultures of olfactory placodes from ovine embryos. Culture medium was collected every 10 min for 8 h to detect pulsatile secretion. In the first experiment, pulsatile secretion was studied in two different sets of cultures after 17 and 24 days in vitro. In the second experiment, a set of cultures was tested after 10, 17 and 24 days in vitro to investigate the development of pulsatile GnRH secretion in each individual culture. This study demonstrated that (i) primary cultures of GnRH neurones from olfactory explants secreted GnRH in a pulsatile manner and that the frequency and mean interpulse duration were similar to those reported in castrated ewes, and (ii) pulsatile secretion was not present at the beginning of the culture but was observed between 17 and 24 days in vitro, indicating the maturation of individual neurones and the development of their synchronization.

Reprogrammed M1 macrophages with inhibited STAT3, STAT6 and/or SMAD3 extends lifespan of mice with experimental carcinoma

2017 ◽  
pp. 4-9
Author(s):  
С.В. Калиш ◽  
С.В. Лямина ◽  
А.А. Раецкая ◽  
И.Ю. Малышев
Keyword(s):  
Tumor Growth ◽  
Culture Medium ◽  
Ehrlich Carcinoma ◽  
Macrophage Phenotype ◽  
M1 Macrophages ◽  
M1 Macrophage ◽  
Ec Cells ◽  
Experimental Carcinoma

Цель исследования. Репрограммирование М1 фенотипа макрофагов с ингибированными факторами транскрипции М2 фенотипа STAT3, STAТ6 и SMAD и оценка их влияния на развитие карциномы Эрлиха (КЭ) in vitro и in vivo. Методика. Рост опухоли иницировали in vitro путем добавления клеток КЭ в среду культивирования RPMI-1640 и in vivo путем внутрибрюшинной инъекции клеток КЭ мышам. Результаты. Установлено, что M1макрофаги и in vitro, и in vivo оказывают выраженный противоопухолевый эффект, который превосходит антиопухолевые эффекты М1, M1, M1 макрофагов и цисплатина. Заключение. М1 макрофаги с ингибированными STAT3, STAT6 и/или SMAD3 эффективно ограничивают рост опухоли. Полученные данные обосновывают разработку новой технологии противоопухолевой клеточной терапии. Objective. Reprogramming of M1 macrophage phenotype with inhibited M2 phenotype transcription factors, such as STAT3, STAT6 and SMAD and assess their impact on the development of Ehrlich carcinoma (EC) in vitro and in vivo . Methods. Tumor growth in vitro was initiated by addition of EC cells in RPMI-1640 culture medium and in vivo by intraperitoneal of EC cell injection into mice. Results. It was found that M1 macrophages have a pronounced anti-tumor effect in vitro , and in vivo , which was greater than anti-tumor effects of M1, M1, M1 macrophages and cisplatin. Conclusion. M1 macrophages with inhibited STAT3, STAT6 and/or SMAD3 effectively restrict tumor growth. The findings justify the development of new anti-tumor cell therapy technology.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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