Acid phosphatases of Ipomoea sp. cultured in vitro. 1. Influence of pH and inorganic phosphate on the formation of phosphatases

1979 ◽  
Vol 57 (7) ◽  
pp. 739-753 ◽  
Author(s):  
M. W. Zink ◽  
I. A. Veliky
Keyword(s):  
Inorganic Phosphate ◽  
Culture Medium ◽  
Soluble Fraction ◽  
Morning Glory ◽  
Acid Phosphatases ◽  
Developmental Patterns ◽  
Low Concentrations ◽  
Shake Flasks ◽  
Influence Of Ph

The levels and the developmental patterns of the two acid phosphatases in Ipomoea sp. (morning glory) were influenced by the pH of the medium and whether the cultures were grown in fermentors or shake flasks. The two enzymes, which appeared in the culture medium, in the soluble fraction, and in the particulate fraction, were derepressed when suspension cultures were grown in a medium containing low concentrations of inorganic phosphate. The addition of up to 4 μmol of phosphate per millilitre to cells grown for 4 days on low phosphate did not repress the synthesis of the enzymes. However, the addition of excess phosphate resulted in a temporary cessation of phosphatase synthesis. Inorganic phosphate appeared to be only one of several factors controlling the levels of the enzymes.

Influence of abscisic acid on the derepression of the acid phosphatases in Ipomoea sp. cultured in vitro

1983 ◽  
Vol 61 (9) ◽  
pp. 2343-2348
Author(s):  
M. W. Zink
Keyword(s):  
Abscisic Acid ◽  
Culture Medium ◽  
Soluble Fraction ◽  
Morning Glory ◽  
Acid Phosphatases ◽  
Developmental Patterns ◽  
Phosphate Deprivation ◽  
Mineral Stress ◽  
High Phosphate

The effect of abscisic acid on the levels and the developmental patterns of the two acid phosphatases in the culture medium, in the soluble fraction, and in the particulate fraction of Ipomoea sp. (morning glory) cultured in vitro depends upon the phosphate status of the cells. Under conditions of mineral stress or phosphate deprivation the enzymes are derepressed and this derepression is suppressed by abscisic acid. No inhibition of the synthesis of the phosphatases by the hormone occurs when the cells are grown under conditions of high phosphate. The significance of the abscisic effect on the derepression of the acid phosphatases is discussed.

Acid phosphatases of Ipomoea sp. cultured in vitro. 2. Influence of gibberellic acid on the formation of phosphatases

1980 ◽  
Vol 58 (20) ◽  
pp. 2171-2180 ◽  
Author(s):  
M.W. Zink
Keyword(s):  
Gibberellic Acid ◽  
Specific Activity ◽  
Soluble Fraction ◽  
Morning Glory ◽  
Original Strain ◽  
Acid Phosphatases ◽  
Developmental Patterns ◽  
Stages Of Growth ◽  
Repressive Effect

The levels and developmental patterns of the two acid phosphatases in the two strains of Ipomoea sp. (morning glory) grown in vitro are influenced differently by gibberellic acid (GA3). In the strain that requires a number of exogenously added hormones for growth (original strain), GA3 appears to show little effect on the specific activity of the phosphatases during the initial 3 days of growth but represses their levels in the growth medium, soluble and particulate fractions upon further growth. The repressive effect of both inorganic phosphate and GA3 on the enzymes appears to be additive. In the strain that does not require exogenously added hormones for growth (NH strain), the level of enzymes in the soluble fraction increases in the presence of GA3 during the early stages of growth and then decreases with culture age. GA3 also elevates the level of the enzymes in the particulate fraction over that in the controls. This elevation increases as phosphate level in the medium is increased. However, GA3 greatly decreases secretion, or leaching of the enzymes from the cells.

scholarly journals STUDIES OF PHOSPHORUS METABOLISM IN MAN: II. A STUDY OF THE PERMEABILITY OF THE HUMAN ERYTHROCYTE TO INORGANIC PHOSPHATE IN VITRO BY THE USE OF RADIOACTIVE PHOSPHATE (P32)

Blood ◽  
1948 ◽  
Vol 3 (12) ◽  
pp. 1472-1477 ◽  
Author(s):  
F. H. L. TAYLOR ◽  
S. M. LEVENSON ◽  
M. A. ADAMS ◽  
MARY KENDRICK
Keyword(s):  
Human Erythrocyte ◽  
Inorganic Phosphate ◽  
Sodium Phosphate ◽  
Specific Activity ◽  
Soluble Fraction ◽  
High Specific Activity ◽  
Phosphorus Metabolism ◽  
Phosphate Exchange ◽  
Inorganic Fraction

Abstract 1. Phosphate exchange in red cells and plasma was studied in vitro using P32 in the form of sodium phosphate as a tracer. 2. No phosphate was added other than the isotopic preparation which was of high specific activity. 3. Inorganic phosphate exchanged freely between the plasma and the erythrocytes at 37.5 C. in a period of four hours. Minimal transfer occurred at 7 C. 4. Most of the added P32 which passed into the erythrocytes during this time remained in the inorganic fraction, less than 15 per cent being found in the organic acid soluble fraction. 5. The specific activity of the inorganic phosphate of the erythrocytes was equal to or greater than that obtaining for the inorganic phosphate of the plasma at the end of the four hour incubation period at 37.5 C.

scholarly journals ISOLATION AND STUDY OF MUTANTS LACKING A DEREPRESSIBLE PHOSPHATASE IN CHLAMYDOMONAS REINHARDI

Genetics ◽  
1975 ◽  
Vol 80 (2) ◽  
pp. 239-250
Author(s):  
R F Matagne ◽  
R Loppes
Keyword(s):  
Alkaline Phosphatase ◽  
Inorganic Phosphate ◽  
Double Mutant ◽  
Culture Medium ◽  
Acid Phosphatases ◽  
Wild Type ◽  
Optimal Activity ◽  
Wide Range ◽  
In The Wild ◽  
Definition Of

ABSTRACT In the green alga Chlamydomonas reinhardi, removal of inorganic phosphate from the culture medium results in the increase of phosphatase activity (derepression) in the wild-type (WT) strain as well as in a double mutant (P2Pa) lacking the two main constitutive acid phosphatases. Following treatment of WT and P2Pa with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), mutants were recovered which display very low phosphatase activities when grown in the absence of phosphate; as shown by electrophoresis, they lack one non-migrating phosphatase (PD mutants). This enzyme is active over a wide range of pH with an optimum at pH 7.5. The comparison of electropherograms from WT and mutants grown on media with or without phosphate allowed us to provide a tentative definition of the pool of derepressible phosphatases in Chlamydomonas : in addition to the neutral phosphatase lacking in PD mutants, Chlamydomonas produces two electrophoretic forms of alkaline phosphatase showing an optimal activity at pH 9.5.

scholarly journals IN VITRO SEED GERMINATION AND PLANT GROWTH OF “CABEÇA-DE-FRADE” (CACTACEAE)

2021 ◽  
Vol 34 (1) ◽  
pp. 1-8
Author(s):  
SHEILA VITÓRIA RESENDE ◽  
ALONE LIMA-BRITO ◽  
GABRIELA TORRES- SILVA ◽  
JOSÉ RANIERE FERREIRA DE SANTANA
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Culture Medium ◽  
Ornamental Plants ◽  
In Vitro Cultivation ◽  
Apical Segment ◽  
Aerial Portion ◽  
Low Concentrations ◽  
Chemical Sterilization

ABSTRACT The genus Melocactus (“cabeça-de-frade”) comprises 32 species in Brazil, of which M. glaucescens and M. paucispinus are threatened with extinction. The present work evaluated the effects of different concentrations of Murashige & Skoog (MS, MS/2 and MS/4) culture medium and sucrose (15 g L-1 and 30 g L-1) on in vitro seed germination and plant growth of M. glaucescens and the efficiency of sterilization with sodium hypochlorite (NaOCl) on in vitro seed germination and plant growth of M. glaucescens when using seeds and M. glaucescens and M. paucispinus when using apical segment of cladode. In M. glaucescens, the final germination at the different MS and sucrose concentrations varied between 53.5 and 68.1% and the best results for in vitro growth were observed with the lowest mineral salt (MS/2 and MS/4) and sucrose (15 g L-1) concentrations, with lengths of the aerial portion of 9.70 and 10.76 mm, respectively. There was no difference in seed germination and plant growth in chemical and autoclave medium. It is concluded that the use of chemical sterilization with NaOCl at low concentrations of salts (MS/2 and MS/4) and sucrose (15 g L-1) are quite advantageous for producing ornamental plants germinated in vitro and/or apical segment of cladode of M. glaucescens andM. paucispinus, representing a reduction of costs for in vitro cultivation of this species.

scholarly journals Nitrate reductase and acid phosphatase activities as affected by inorganic phosphate in corn roots

2014 ◽  
Vol 52 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Marie Kummerova ◽  
Józef Buczek
Keyword(s):  
Acid Phosphatase ◽  
Nitrate Reductase ◽  
Nutrient Solution ◽  
Inorganic Phosphate ◽  
Acid Phosphatases ◽  
Weak Effect ◽  
Corn Roots ◽  
Nr Activity

The deficieny of inorganic phosphate in nutrient solution reduces by about 50 per cent NO<sub>3</sub>- absorption in corn seedlings, it decreases both <em>in vitro</em> and in vivo nitrate reductase (NR) activity, as well the potential and actual NR level and has a very weak effect on NR induction. Acid phosphatases activities increase in corn roots when the plants are grown in nutrient solution without phosphorus. We suggest that inorganic phosphate is required mainly for maintenance of NR activity rather, than for induction <em>in vivo</em> of nitrate reductase. It is not excluded that deficiency of inorganic phosphate in root tissue may be partly supplemented as the result of enhanced acid phosphatase activity.

Les bruyères in vitro. VII. pH du milieu de culture et morphogenèse de L'Erica ×darleyensis. Méthode d'interprétation des données

1989 ◽  
Vol 67 (1) ◽  
pp. 156-160
Author(s):  
F. Beaujard ◽  
J. D. Viémont
Keyword(s):  
Plant Development ◽  
Culture Medium ◽  
Rhythmic Growth ◽  
Proper Interpretation ◽  
Influence Of Ph

Erica ×darleyensis grown in vitro presents a rhythmic growth. A method of study has been devised to relate the pH evolution of the culture medium and plant development. We found this method useful for proper interpretation of the influence of pH on our cultures.

STIMULATORY EFFECT OF FSH IN VITRO ON THE EXTRACELLULARLY ACTIVE CYCLIC AMP PHOSPHODIESTERASE IN THE PREPUBERTAL RAT OVARY

1976 ◽  
Vol 81 (2) ◽  
pp. 563-573 ◽  
Author(s):  
Gunnar Selstam ◽  
Sten Rosberg
Keyword(s):  
Cyclic Amp ◽  
Incubation Medium ◽  
Inorganic Phosphate ◽  
Kinetic Studies ◽  
Bicarbonate Buffer ◽  
Low Concentrations ◽  
Rat Ovary ◽  
Camp System ◽  
Burk Plot

ABSTRACT Intact prepubertal rat ovaries were incubated with radioactively labelled adenosine 3,′5′-cyclic monophosphate (cAMP) in Krebs bicarbonate buffer containing glucose. The rate of degradation of cAMP was determined by measuring the radioactivity in the medium after precipitation with Ba(OH)2 and ZnSO4. The fate of the nucleotide was followed by measuring the products in the incubation medium. Paper chromatography was used for the separation and identification of these products. It was found that cAMP was degraded to AMP, which in turn was degraded to inorganic phosphate (Pi) and adenosine. An uptake of labelled products was also observed. NIH-FSH-S9 (10 and 100 μg/ml), but not NIH-LH-B8 (0.1–100 μg/ml), increased the degradation of cAMP. Concomitantly, an increased accumulation of labelled adenosine and Pi as well as an increased uptake of labelled products were seen. Kinetic studies with low concentrations of cAMP (0.125–0.025 μmol/l) revealed an apparent Km value of 0.12 μmol/l for the phosphodiesterase (PDE) activity. FSH significantly changed the slope of the curve in the Lineweaver-Burk plot by increasing the PDE activity. The increased PDE activity in the presence of FSH is discussed in relation to earlier findings of differences in action between LH and FSH on the cAMP system in the prepubertal rat ovary.

scholarly journals LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of Phalaenopsis

Horticulturae ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 34 ◽  
Author(s):  
Hasan Mehraj ◽  
Md. Meskatul Alam ◽  
Sultana Umma Habiba ◽  
Hasan Mehbub
Keyword(s):  
Culture Medium ◽  
In Vitro Regeneration ◽  
Fresh Weight ◽  
Chlorocholine Chloride ◽  
Light Emitting ◽  
In Vitro Organogenesis ◽  
Low Concentrations ◽  
Plant Culture ◽  
The Impact

Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in Phalaenopsis ‘Fmk02010’. We applied 15 LEDs combined with three CHO sources and five CCC concentrations in the study. Organogenesis of PLBs was very poor in maltose both for the number of PLBs and their fresh weight (FW) compared to media containing sucrose and trehalose. Sucrose was the best CHO source under the red-white (RW) LED for the in vitro organogenesis of PLBs (PLBs: 54.13; FW: 0.109 g), while trehalose was best under the blue-white (BW) LED (PLBs: 36.33, FW: 0.129 g). The red-blue-white (RBW)-trehalose combination generated a suitable number of PLBs (35.13) with the highest FW (0.167 g). CCC at 0.01, 0.1, and 1 mgL−1CCC had no effect on PLB formation or FW, but 10 mg L−1 reduced both. RW-sucrose, BW-trehalose, and RBW-trehalose were the best combinations for PLB organogenesis. The addition of low concentrations of CCC in the plant culture medium are unnecessary.

scholarly journals Response of Four Lemon (Citrus limon L. Burm) Cultivars Cultured in vitro to BAP, KIN and 2,4- D Combinations

2017 ◽  
Vol 27 (1) ◽  
pp. 39-50
Author(s):  
Abdul Mohsin Radah Obiad Al Sayed Abdul Mohsin Radah Obiad Al Sayed
Keyword(s):  
Culture Medium ◽  
Citrus Limon ◽  
High Concentration ◽  
Low Concentrations ◽  
Micro Propagation ◽  
King Abdulaziz University ◽  
High Concentrations ◽  
One Year ◽  
The One

The present study was carried out in the lab. of plant tissue culture at King Abdulaziz University to test the response of four lemon cultivars to micro-propagation using BAP, Kin and 2,4- D combinations. The used explants in this study were intermodal segments and collected from the one year old lemon seedlings which obtained from the Citrus Research Center, Najran, Saudi Arabia. The experiments were laid out in a split plot design using 4 replicates. The results revealed that there were significant differences due to genotypic and growth regulators effects and their interaction for all measured parameters except no of days to buds sprouting. Explants of ‘Shehri’ registered maximum values of no. of days to buds sprouting with 0.5mg/l-1 Kin +0.5mg/l-1 2,4-D, % sprouted buds with 0.5mg/l-1 BAP, % dead shootlets with 2mg/l-1 BAP+0.5mg/l-1 2-,4- D and length of primary shoots (mm) 1mg/l-1 BAP+0.5mg/l-1 2-,4- D. Shoots of ‘AlnEurka’ formed the highest no. of leaves with 0.5mg/l-1 BAP+1mg/l-1 2-,4- D. Low responses were observed for explants from ‘Shaary’, ‘Banzahir’ and ‘Aln-Eurka’ on culture medium supplemented with high concentration of BAP alone or with combination of 2,4- D. There were observed no sprouted buds for explants of ‘Shehri’ on culture medium complemented with high concentrations of Kin in combinations with 2,4- D. Lemon explants were successfully in vitro propagated using intermodal segments and combinations of BAP, Kin and 2,4- D at low concentrations.

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