Growth stimulation of apple trees in unsterilized soil under field conditions with VA mycorrhiza inoculation

1981 ◽  
Vol 59 (11) ◽  
pp. 2003-2008 ◽  
Author(s):  
Christian Plenchette ◽  
Valentin Furlan ◽  
J. André Fortin

Apple seedlings germinated and grown under greenhouse conditions were inoculated with endomycorrhizal roots originating from a nursery grown Fraxinus prior to outplanting into unsterilized field soil. Uninoculated seedlings, in soil either unamended or amended with 100 kg/ha P as superphosphate, were controls. Shoot length, leaf surface, root volume, stem diameter, and dry mass of inoculated plants were all significantly greater than both fertilized and unfertilized controls. No significant difference could be observed in foliar mineral content but the level of N, P, Ca, Cu, and possibly K were higher in the roots of inoculated plants than in controls. Although shoot length of the phosphorus fertilized plants exceeded that of the unfertilized controls (p < 0.05), all the other growth parameters remained unchanged by the addition of P. Intracortical vesicles were absent or very uncommon in the roots of both fertilized and unfertilized control plants whereas they were very abundant in the roots of inoculated plants.It is concluded that inoculation of seedlings previous to field planting in unsterilized phosphorus-poor soil can lead to a significant growth stimulation in spite of the presence of a natural endomycorrhizal flora.

Author(s):  
Hanna Ibiapina De Jesus ◽  
Maria Luiza De Souza Medeiros ◽  
Maria De Fátima de Queiroz Lopes ◽  
Bruno De Souza Oliveira ◽  
Galileu Medeiros Da Silva ◽  
...  

Objective: Evaluate the development and gas exchange of pre-sprouted sugarcane seedlings in different substrates. Study Design: A randomized complete block design was used with two genotypes, three types of substrate (2 x 3), four replicates and five plants per replicate, totaling 120 plants. Location and Duration of Study: Department of Crop Sciences of the Center for Agrarian Sciences, Universidade Federal de Paraíba in the city of Areia, Brazil, between August and October 2018. Methods: The following were the treatments: T1 – genotype 1 in commercial substrate; T2 – genotype 1 in commercial substrate + bovine manure; T3 – genotype 1 in bovine manure + sand; T4 – genotype 2 in commercial substrate; T5 – genotype 2 in commercial substrate + bovine manure; T6 – genotype 2 in bovine manure + sand. Variables analyzed: height, diameter, dry mass of shoot and root and physiological response of seedlings. Results: A significant difference at the 1% level was found for the dry matter of the seedling roots among treatments with difference substrates. A significant difference at the 1% level was also found for shoot length and a significant interaction at the 5% level was found between genotype and substrate for this characteristic. Significant effects were found with regard to the rate of photosynthesis, stomatal conductance, transpiration and carboxylation efficiency. Conclusion: The substrate with manure + sand favored the accumulation of root dry matter and shoot length. Genotype 1 exhibited its best performance with regard to photosynthesis and stomatal conduction in the treatment with the commercial substrate and demonstrated greater carboxylation efficiency than genotype 2, independently of the substrates. The pre-sprouted seedling production system needs to be explored further to obtain greater information regarding the nutritional requirements and characteristics of the genotypes.


2018 ◽  
Vol 40 (1) ◽  
pp. 39073
Author(s):  
Caroline Barbeiro ◽  
Taysi Pereira Firmino ◽  
André Henrique Oler de Novais ◽  
Mariza Barion Romagnolo ◽  
Lindamir Hernandez Pastorini

 Albizia niopoides (Bentham) Burkart, known as farinha seca, is an arboreal, deciduous and pioneer plant that can be found in the semi-deciduous forest in the Paraná basin. The study on the germination and growth of the plant is fundamental for better knowledge about the establishment and regeneration of the species. Aiming to verify the germination of A. niopoides and subsequent growth, green seeds and brown seeds were germinated in a germination chamber and growth parameters were evaluated at 60, 90, and 180 days after transplanting (DAT) in a greenhouse. There was no significant difference in germination percentage between green and brown seeds. However, lower levels of mean germination times and higher levels of germination speed index were observed for brown seeds. At 180 DAT, plants showed higher shoot dry mass, root dry mass, total dry mass, number leaves and height, with no significant difference between 60 and 90 DAT for total dry mass, root dry mass and root length. The values of stem height/diameter and Dickson quality index, associated to the other growth parameters, indicate quality of the seedlings for field planting at 180 DAT. 


2016 ◽  
Vol 41 (3) ◽  
pp. 451-460 ◽  
Author(s):  
MN Hasan ◽  
MA Salam ◽  
MMI Chowdhury ◽  
M Sultana ◽  
N Islam

The experiment was conducted at the Seed Laboratory, Department of Agronomy, Bangladesh Agricultural University, Mymensingh during the period from September to October, 2011 to study the effect of chemical priming of seed on germination and growth of rice seedling cv. BRRI dhan 29. Seeds were soaked in 3% and 5% solutions of CaCl2, ZnSO4 and KCl for 24, 30, 36, 42, 48, 54, 60 hours, respectively. A control was maintained where seeds were subjected to no priming treatment. The experiment was laid out in a Completely Randomized Design with three replications. Primed seeds were tested for germination and vigour on sandy soil media (60:40) in petridish under field capacity. Seed quality tests included mean germination time, shoot length, shoot dry mass, root length and root dry mass. The results revealed that priming treatments had significant influence on germination and all the growth parameters of rice seedlings. Priming with 3% ZnSO4 for 30 hours showed the highest germination percentage and the lowest mean germinaion time. Priming with 5% KCl for 54 hours showed the highest root length while 5% of the same solution for 24 hours showed the highest root dry mass. On the contrary, seeds having no priming treatment showed the lowest values for germination, vigour index, shoot length,shoot dry mass, root length and root dry mass and the highest mean germination time. The present study concludes that rice seed cv. BRRI dhan29 could be primed with 3% ZnSO4 solution for 30 hours for improving germination and seedling growth.Bangladesh J. Agril. Res. 41(3): 451-460, September 2016


2021 ◽  
Vol 58 (04) ◽  
pp. 1291-1299
Author(s):  
Muhammad Usman Afzal

Citrus is known as a major fruit due to its high nutritional value and adaptability in tropical and sub-tropical regions. Among diseases, citrus dieback is one of the most threatening diseases in which overall plant growth is reduced. Major causes are Colletotrichum gloeosporioides and citrus nematode (Tylenchulus semipenetrans) with 15-35% losses all over the world. Plant material was established adopting sanitary measures in earthen pots (12-inch diameter). Fresh culture of C. gloeosporioides and T. semipenetrans was prepared for the inoculation by following the standard procedures. In the first set, rootstocks were inoculated with 200mL of water having spore suspension of C. gloeosporioides while in in the second set, inoculation of T. semipenetrans was done by using 45 mL of water suspension having 2000 freshly hatched juveniles per pot, while at the rate of 1 × 107 spores/mL per plant. In the third set, the interaction of C. gloeosporioides and T. semipenetrans was studied by inoculating selected citrus rootstocks by both pathogens. After four months of inoculation, data were recorded on plant disease index (PDI) along with plant growth parameters (root weight, shoot weight, shoot weight, shoot length and number of leaves). Trifoliate orange and cox mandarin hybrid showed resistance against the development of T. semipenetrans while rough lemon, C-35 Citrange and sour orange were found susceptible. There was a significant difference in plant growth parameters between inoculated and healthy plants. Root weight and shoot weight decreased by 8.98g and 11.53g, while root length and shoot length decreased by 7.29cm and 13.5cm respectively as compared to control treatments in most susceptible rootstocks. Per cent Branch Infection (PBI) and per cent Disease Index (PDI) were maximum (71.52, 37) per cent on rough lemon respectively. Results regarding combined inoculation of C. gloeosporioides and T. semipenetrans showed that there was a significant difference in plant growth parameters between inoculated and healthy plants. In rough lemon, Root weight and shoot weight decreased by 13.86 and 20.57g respectively in diseased and healthy plants. Root length and shoot length decreased by 8.37 and 20.04cm respectively as compared to control treatments in most susceptible rootstocks. Overall results depicted that inoculation of both pathogens reduced plant growth more severely as compared to their individual application.


1992 ◽  
Vol 43 (5) ◽  
pp. 1067 ◽  
Author(s):  
LD Prior ◽  
AM Grieve ◽  
BR Cullis

Effects of salinity on the mineral composition, growth and physiology of field-grown sultana grapevines were studied by adding NaCl solution to River Murray water. Five salt treatments, ranging between 0.37 and 3.47 dS m-l, were applied for six seasons. Petiole levels of Cl and Na were better predictors of yield than were lamina levels. There was no evidence of safe threshold salinity levels, so grapevine growers should aim to keep tissue salinity levels as low as possible, certainly below 1.5% and 0.5% for petiole Cl and Na respectively. Yield was affected not only by the salinity of the tissue in the current season, but also by tissue salinity in the preceding seasons. A model was developed which included previous tissue salinity measurements. Salt treatment reduced all growth parameters measured-pruning weight, shoot length, cane number and leaf and petiole weight. Decreases in stomatal conductance and photosynthesis were measured in the field in leaves of salinised vines, but leaf water potential was not affected. These decreases in photosynthetic rate are the probable cause of the severe yield and growth reductions in salinised vines.


2021 ◽  
Vol 9 (6) ◽  
pp. 63
Author(s):  
Payam Farzad ◽  
Ted Lundgren ◽  
Adel Al-Asfour ◽  
Lars Andersson ◽  
Christer Dahlin

This study was undertaken to investigate the integration of titanium micro-implants installed in conjunction with previously dentin-grafted areas and to study the morphological appearance, mineral content, and healing pattern of xenogenic EDTA-conditioned dentin blocks and granules grafted to cavities in the tibial bone of rabbits. Demineralized and non-demineralized dentin blocks and granules from human premolars were implanted into cavities prepared on the lateral aspects of the tibias of rabbits. After a healing period of six months, micro-implants were installed at each surgical site. Histological examinations were carried out after 24 weeks. Characterization of the EDTA-conditioned dentin blocks was performed by means of light microscopy, dental X-rays, scanning electron microscopy, and energy dispersive X-ray analysis (EDX). No implants were found to be integrated in direct contact with the dentin particles or blocks. On the EDTA-conditioned dentin surface, the organic marker elements C and N dominated, as revealed by EDX. The hydroxyapatite constituents Ca and P were almost absent on the dentin surface. No statistically significant difference was observed between the EDTA-conditioned and non-demineralized dentin, as revealed by BIC and BA. The bone-inductive capacity of the dentin material seemed limited, although demineralization by means of EDTA indicated higher BIC and BA values in conjunction with the installed implants in the area. A 12 h EDTA treatment did not fully decalcify the grafts, as revealed by X-ray analysis.


1995 ◽  
Vol 46 (3) ◽  
pp. 583 ◽  
Author(s):  
RJQ Tarr

Growth rates of a number of Haliotis midae populations around the South African coast were studied by means of tagging. These populations ranged from the cool waters of the western coast to the more temperate environment of the eastern Cape. Standard von Bertalanffy growth curves were fitted and growth parameters derived. These ranged from 0.19 to 0.25 for K, the average rate at which L∞ is approached, and from 156 to 173 for L∞, the average theoretical maximum length. These indicate far higher growth rates than were previously published for this commercially fished species, and the reasons for this difference are discussed. The expectation that growth rates would be fastest in the warmer eastern Cape waters was not realized, there being no significant difference in growth between the Bird Island population on the eastern coast and the Robben Island population on the western coast. These new growth parameters indicate that H. midae in the commercial fishery grounds is attaining sexual maturity some four years earlier, and the minimum legal size some five years earlier, than previously considered. This has considerable significance for modelling studies presently underway. Movement of a small population of adult H. midae was studied over a three-year period, after which 47% of the original abalone were still present on the study site. Of these, 81.5% still occupied exactly the same position on the rocks. This indicates that H. midae that have located an optimum habitat, and that are not disturbed, tend not to move.


Author(s):  
P. Masilamani ◽  
T. Eevera ◽  
T. Ramesh ◽  
S. Venkatesan

Background: The present investigation is an attempt to study the effect of different harvesting and threshing methods on germination and seedling vigour of dhaincha. The use of a combine harvester to harvest dhaincha will be an effective alternative method that has not been widely tried. However, work on different methods of harvesting and threshing of dhaincha is limited. Hence, a study was conducted to evaluate the impact of various harvesting and threshing methods on germination and seedling vigour of dhaincha. Methods: This study was conducted at Anbil Dharmalingam Agricultural College and Research Institute, TNAU, Tiruchirappalli, Tamil Nadu. The dhaincha crop was harvested and threshed using four different methods viz., manual harvesting and manual threshing, manual harvesting and threshing by tractor treading, manual harvesting and mechanical threshing and harvesting and threshing by combine harvester. The resultant seeds were tested for mechanical damage and germination potential. The experiment was laid in completely randomized block design. Germination was tested by roll towel method using 100 seeds in four replications. Germination percentage, root and shoot length were measured in seven days after sowing from ten randomly selected seedlings in each replication. For the estimation of dry matter production, ten seedlings were selected at random and kept in a hot air oven maintained at 85oC for 24 hours after measuring their root and shoot length and vigour index was calculated. Mechanical damage to seeds was observed by ferric chloride test. Seed recovery per cent was calculated based on the 100 kgs of dhaincha seeds were cleaned and graded treatment wise using cleaner cum grader and the seeds retained on the bottom sieve were weighed and expressed as per cent of total quantity of seed. Result: The results revealed that the significant difference was found among the different harvesting and threshing methods. The seeds harvested and threshed by manual method recorded 85 per cent germination followed by seeds harvested manually and threshed by mechanical threshing (84 per cent) and seeds harvested manually and threshed by tractor treading (80.5 per cent). The lowest germination of 80.0 per cent was recorded by combine harvesting. From this study, it could be inferred that combine harvester is a modern method for harvesting of dhaincha that saves time and labour when compared to all other methods.


2015 ◽  
Vol 33 (3) ◽  
pp. 405-412 ◽  
Author(s):  
P. V. SILVA ◽  
P. A. MONQUERO ◽  
F. B. SILVA ◽  
N. C. BEVILAQUA ◽  
M. R. MALARDO

ABSTRACT This study aimed to understand the influence of sowing depth and the amount of sugarcane straw on the emergence of weed species Luffa aegyptiaca Miller (Cucurbitaceae); Mucuna aterrima Piper & Tracy (Fabaceae - Leguminosae) and Ricinus communis (Euphorbiaceae). A completely randomized design with a 5 x 4 x 3 factorial layout with four replications was used, at five sowing depths (0, 2, 4, 8 and 10 cm), four different amounts of sugarcane straw (0, 5, 10 and 15 t ha-1) and three different evaluation periods (7, 14 and 21 days after sowing). After sowing, different amounts of sugarcane straw (0, 5, 10 and 15 t ha-1) were deposited on soil. Seedling emergence was analyzed at 7, 14 and 21 days after sowing, counting the number of seedlings that had emerged. At the end of the trial, weed height (cm), leaf area (cm2) and shoot dry mass (g) were measured. In relation to emergence ability, studied species presented different responses according to sowing depth and to the amount of sugarcane straw deposited on the soil. For the L.aegyptiacaand M.aterrima, no significant difference was observed in the interaction between depth and sugarcane straw, showing the adaptation of these species to no-burn sugarcane system. For R.communis, seeds placed at 0 cm of sugar cane straw depth were observed to favor the emergence of seedlings.


2006 ◽  
Vol 43 (4) ◽  
pp. 305-309
Author(s):  
Camila Carbone Prado ◽  
Roberto José Negrão Nogueira ◽  
Antônio de Azevedo Barros-Filho ◽  
Elizete Aparecida Lomazi da Costa-Pinto ◽  
Gabriel Hessel

BACKGROUD: Chronic liver diseases in childhood often cause undernutrition and growth failure. To our knowledge, growth parameters in infants with neonatal cholestasis are not available AIM: To evaluate the nutritional status and growth pattern in infants with intrahepatic cholestasis and extrahepatic cholestasis. PATIENTS AND METHODS: One hundred forty-four patients with neonatal cholestasis were followed up at the Pediatric Gastroenterology Service of the Teaching Hospital, State University of Campinas, Campinas, SP, Brazil, in a 23-year period, from 1980 to 2003. The records of these patients were reviewed and patients were classified into two groups, according to their anatomical diagnosis: patients with intrahepatic cholestasis - group 1, and patients with extrahepatic cholestasis - group 2. Records of weight and height measurements were collected at 4 age stages of growth, in the first year of life: 1) from the time of the first medical visit to the age of 4 months (T1); 2) from the 5th to the 7th month (T2); 3) from the 8th to the 10th month (T3); and 4) from the 11th to the 13th month (T4). The weight-by-age and height-by-age Z-scores were calculated for each patient at each stage. In order for the patient to be included in the study it was necessary to have the weight and/or height measurements at the 4 stages. Analyses of variance and Tukey's tests were used for statistical analysis. Repeated measurement analyses of variance of the weight-by-age Z-score were performed in a 60-patient sample, including 29 patients from group 1 and 31 patients from group 2. The height-by-age data of 33 patients were recorded, 15 from group 1 and 18 from group 2 RESULTS: The mean weight-by-age Z-scores of group 1 patients at the 4 age stages were: T1=-1.54; T2=-1.40; T3=-0.94; T4=-0.78. There was a significant difference between T2 X T3 and T1 X T4. The weight-by-age Z-scores for group 2 patients were :T1=-1.04; T2=-1.67; T3=-1.93 and T4=-1.77, with a significant difference between T1 X T2 and T1 X T4. The mean weight-by-age Z-scores also showed a significant difference between group 1 and group 2 at stages T3 and T4. The mean height-by-age Z-scores at the four stages in group 1 were: T1=-1.27; T2=-1.16; T3=-0.92 and T4=-0.22, with a significant difference between T3XT4 and T1XT4. The scores for group 2 patients were: T1=-0.93; T2=-1.89; T3=-2.26 and T4=-2.03, with a significant difference between T1XT2 and T1XT4. The mean height-by-age Z-scores also showed a significant difference between group 1 and group 2 at T3 and T4 CONCLUSION: The weight and height differences between the groups became significant from the 3rd measurement onward, with the most substantial deficit found in the extrahepatic group. In this group, there is evidence that the onset of weight and height deficit occurs between the first and second evaluation stages.


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