Fasciation d'extrémités caulinaires du Celosia cristata (Amarantacées) cultivées in vitro

1981 ◽  
Vol 59 (8) ◽  
pp. 1367-1372 ◽  
Author(s):  
D. Driss-Ecole
Keyword(s):  
Indoleacetic Acid ◽  
Leaf Primordia ◽  
Root Initiation ◽  
Salt Mixture ◽  
Shoot Apices ◽  
In Vitro Development ◽  
Long Period ◽  
Celosia Cristata ◽  
Vitro Development

In vitro development of fasciation was achieved from the excised shoot apices of Celosia cristata in a 16-h photoperiod. An 8-h photoperiod produced no development. The explants consisted of the meristematic dome with two leaf primordia. The best results were obtained with a nutrient medium composed of Murashige and Skoog salt mixture, 30 g/L sucrose, 5 g/L agar, and 1 mg/L indoleacetic acid (IAA). Isolated shoot apices developed callus at the bases of the explants and numerous leaves on one, two, or three flattened and fasciated meristems. Root initiation occurred sometimes but only after a long period of culture.

In vitro development of isolated leaf primordia of Matteuccia struthiopteris

1985 ◽  
Vol 63 (5) ◽  
pp. 916-919 ◽  
Author(s):  
P. Von Aderkas ◽  
G. Hicks
Keyword(s):  
Shoot Apex ◽  
Root Formation ◽  
Leaf Primordia ◽  
The Other ◽  
Secondary Development ◽  
In Vitro Development ◽  
Morphological Complexity ◽  
Developmental Fate ◽  
Vitro Development

Primordia (P2–P6) at the shoot apex were excised and cultured on Knudson's medium for a period of 4 weeks. The majority of primordia developed as leaves. The length, mass, and morphological complexity of these leaves were related to initial primordium age and height. There was a consistent trend toward the production of shorter, lighter, and less complex leaves from the younger, smaller initial explants. A second set of experiments traced the developmental fate of isolated primordia (P1–P4) over a longer period of time (12 weeks). Various kinds of secondary development were observed including bud and root development. Bud numbers decreased with primordial age. On the other hand, the rate of root formation increased.

99 SURVIVAL RATE AND IN VITRO DEVELOPMENT OF IN VIVO-PRODUCED AND CRYOPRESERVED DOG EMBRYOS

2010 ◽  
Vol 22 (1) ◽  
pp. 208
Author(s):  
M. R. Luz ◽  
C. C. Holanda ◽  
J. J. Pereira ◽  
N. S. Teixeira ◽  
R. Vantini ◽  
...  
Keyword(s):  
Survival Rate ◽  
Room Temperature ◽  
Reproductive Technologies ◽  
Water Bath ◽  
Embryo Cryopreservation ◽  
In Vitro Development ◽  
Long Period ◽  
Vitro Development

Dogs have been used as an experimental model for human genetic diseases and for research applied to endangered Canidae. Moreover, application of assisted reproductive technologies (ART) by dog breeders is increasing. The aim of this study was to evaluate the survival rate and in vitro development of in vivo-produced and cryopreserved dog embryos. Seven cross-bred bitches were submitted to ovariohysterectomy 12 days after first mating or artificial insemination, and embryos were recovered by uterine horn flushing with 30 mL of PBS/horn (Nutricell, Campinas, SP, Brazil). Grade 1 and 2 morulae (MO; n = 7; IETS) and blastocysts (BL; n = 14) were frozen. For freezing, embryos were immersed in glycerol 10% (GLY; Nutricell, Campinas, SP, Brazil) or ethylene glycol 3,0 M (EG; Nutricell, Campinas, SP, Brazil) for 10 min. Straws were placed in the machine at -7.0°C (TK 3000, Uberaba, MG, Brazil), and equilibrated for 2 min. Seeding was performed at -7.0°C, and another equilibrium period of 2 min was performed. A cooling rate of -0.5°C/min until -32.0°C was used. Embryos were stored in N2L until thawing. Prior to in vitro culture, embryos were removed from N2L, kept at room temperature for 10 s, and put in a water bath at 25°C for 20 s. Embryos frozen in GLY were washed for 5 min in each thawing solution for cryoprotectant removal (0.6 M sacarose + glycerol 5%; 0.6 M sacarose + glycerol 2.5% and 0.6 M sacarose; Nutricell, Campinas, SP, Brazil). After that, embryos were washed 10 times in holding solution (Holding Plus, Bioniche, Pullman, WA, USA). Embryos frozen in EG were kept at room temperature for 10 s, put in a water bath at 25°C for 20 s, and were directly washed 10 times in holding solution. Comparison among groups was performed by ANOVA. After thawing, 9/11 (81.8%) embryos frozen in EG had rupture of zona pelucidae and 2/11 (18.2%) were intact, whereas 9/10 (90.0%) embryos frozen in GLY were intact (P < 0.05). All intact embryos (n = 11) were morphologically normal and were transferred to SOF medium (Nutricell, Campinas, SP, Brazil), cultured for 168 h at 38.3°C, and evaluated at 24-h intervals. After the last evaluation, for both cryoprotectants, hatching rate was 0.0%, but all embryos were morphologically normal. The results of this study suggest that dog embryos have a high in vitro survival rate in standard protocols used for mammalian embryo cryopreservation when glycerol 10% is used as cryoprotectant and that a long period of culture (possibly more than 10 days) is required for dog embryo hatching. On the other hand, this long period for in vitro hatching may reflect the delayed hatching and implantation that occurs in vivo in this species. Financial by FAPES/MCT/CNPq/CT-INFRA n. 19/2006; Processo n. 36600737/2007, ES- Brasil.

In vitro development of human triploid zygotes reconstructed by pronuclear transfer

2002 ◽  
Vol 78 ◽  
pp. S180-S181
Author(s):  
John Zhang ◽  
Yi Ming Shu ◽  
Lewis C Krey ◽  
Hui Liu ◽  
Guang Lun Zhuang ◽  
...  
Keyword(s):  
In Vitro Development ◽  
Pronuclear Transfer ◽  
Vitro Development

Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

2021 ◽  
pp. 106767
Author(s):  
Gizele A.L. Silva ◽  
Luana B. Araújo ◽  
Larissa C.R. Silva ◽  
Bruna B. Gouveia ◽  
Ricássio S. Barberino ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Phosphatidylinositol 3 Kinase ◽  
In Vitro Development ◽  
Vitro Development ◽  
Kinase Pathway ◽  
Phosphatidylinositol 3

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

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