Mise en évidence d'activités enzymatiques au niveau de l'haustorium d'une phanérogame parasite : Striga hermonthica (Scrophulariaceae)

1979 ◽  
Vol 57 (22) ◽  
pp. 2564-2571 ◽  
Author(s):  
Amadou Tidiane Ba ◽  
Guy Kahlem
Keyword(s):  
Acid Phosphatase ◽  
Succinate Dehydrogenase ◽  
Cytochrome Oxidase ◽  
Active Transport ◽  
Enzymatic Activities ◽  
Striga Hermonthica ◽  
Radial System ◽  
Host Penetration ◽  
Penetration Mechanism

Acid phosphatase, ATPase, peroxidase, succinate dehydrogenase, and cytochrome oxidase activities have been demonstrated in the haustorium of Striga hermonthica, a parasitic phanerogam on millet (Pennisetum typhoideum var. Sonna 3). Their localization has been specified and their possible role discussed. These enzymatic activities seem to be related to the host penetration mechanism and also with the process of active transport of solutes between host and parasite. A tracheid radial system has also been discovered in the mature haustorium.

scholarly journals ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe
Keyword(s):  
Acid Phosphatase ◽  
Contaminated Soil ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Enzymatic Activities ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Indigenous Bacteria ◽  
Rock Phosphate Solubilization ◽  
Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

scholarly journals Effect of Pesticides on Enzymatic Activity in Soil

2018 ◽  
Vol 75 (2) ◽  
pp. 80
Author(s):  
Maria- Mihaela MICUȚI ◽  
Liliana BĂDULESCU ◽  
Florentina ISRAEL-ROMING
Keyword(s):  
Acid Phosphatase ◽  
Enzymatic Activity ◽  
Biological Indicator ◽  
Enzymatic Activities ◽  
Soil Enzymatic Activity ◽  
Alkaline And Acid Phosphatase

The focus of this article is to provide informations about soil enzymatic activity as a biological indicator for impacts of pesticides on soils. In this experiment, an ecological soil was treated with two types of fungicide (Ridomil Gold and Bravo 500) and two of insecticides (Mospilan 20SG and Vertimec 1.8% EC). The pesticides were assessed for their effect on different enzymatic activities. They were administrated over a 28 days period and the samples of soil were taken once every 7 days and analyzed in the laboratory. For each sample was determined the enzymatic activity, pH, humidity. The enzymatic activity was assessed using colorimetrical methods. Enzymes chosen for this study were cellulase, amylase, xylanase, urease, alkaline and acid phosphatase. Results shown that the enzymatic activity can increase or decrease when the soil was treated with the fungicides and insecticides chosen for this experiment.

A histochemical study of starch, lipids, and certain enzymes in senescing rose stems

1977 ◽  
Vol 55 (6) ◽  
pp. 617-624 ◽  
Author(s):  
J. M. Molnar ◽  
E. V. Parups
Keyword(s):  
Acid Phosphatase ◽  
Cytochrome Oxidase ◽  
Lipid Content ◽  
Histochemical Study ◽  
Succinic Dehydrogenase ◽  
Sugar Solution ◽  
Lipid Peroxidase ◽  
High Level ◽  
The Rose ◽  
Cut Rose

The starch, lipid, cytochrome oxidase (EC 1.9.3.1), succinic dehydrogenase (EC 1.3.99.1), peroxidase (EC 1.11.1.7), and acid phosphatase (EC 3.1.3.2) levels were determined periodically by histochemical methods in transverse sections of cut stem of the rose, Rosa hybrida L. cv. Forever Yours, kept in water or in an aqueous preservative solution containing 4% sucrose, 100 ppm sodium isoascorbate, and 100 ppm 8-hydroxyquinoline sulfate. Senescence of the cutrose stem, including leaves and flowers, was delayed by use of the sugar solution. The levels of cytochrome oxidase and succinic dehydrogenase were not significantly affected by either of the treatments. Starch, lipid, peroxidase, and acid phosphatase levels decreased in the tissues of rapidly senescing stems kept in water. In tissues where senescence was retarded by use of sugar solutions the lipid content and peroxidase were maintained at a relatively high level; starch, and acid phosphatase levels steadily increased. It is suggested that in cut rose stems, the onset or retardation of senescence is not related to the activities of acid phosphatase or peroxidase.

scholarly journals GENDER AND AGE ASPECTS OF BIOENERGETICS PROCESSES IN EXPERIMENTAL PASSIVE TOBACCO SMOKING AND MONOSODIUM GLUTAMATE ADMINISTRATION

2019 ◽  
Vol 4 (2) ◽  
pp. 79-86
Author(s):  
A. V. Rutska ◽  
I. Ya. Krynytska
Keyword(s):  
Succinate Dehydrogenase ◽  
Cytochrome Oxidase ◽  
Tobacco Smoke ◽  
Molecular Mechanisms ◽  
Monosodium Glutamate ◽  
Food Additives ◽  
Significant Inhibition ◽  
Male Rats ◽  
Control Group ◽  
Mitochondrial Enzymes

Background. Active smoking and exposure to passive smoke are responsible for more than 5 million deaths each year. At the same time, a characteristic feature of present food technologies is the use of food additives that are not always safe for human health, such as monosodium glutamate (MSG). Objective. The aim of the research was to determine the changes in mitochondrial enzymes activity in rats in case of passive tobacco smoke combined with prolonged administration of MSG in the sex and age aspects. Methods. The evaluation of bioenergetics processes in the mitochondria of circulating neutrophils was carried out using succinate dehydrogenase (SDG) and cytochrome oxidase (CO) activity.  Results. Passive tobacco smoke combined with the MSG administration in mature male-rats is accompanied by a significant inhibition of bioenergetics processes, as evidenced by a decrease in succinate dehydrogenase activity by 47.1% (p<0.001) compared to the intact animals, which is by 27.9% (p<0.001) below this index in case of the isolated effect of tobacco smoke and reduction of cytochrome oxidase activity by 27.5% (p<0.001) compared to the control group. Conclusions. Thus, the findings suggest that low dose intake of monosodium glutamate enhances the ability of tobacco smoke to disrupt the cell's bioenergetics processes by affecting the respiratory chain function and generation of ATP. Therefore, it is advisable to investigate the established toxic doses of E621, as well as to study the molecular mechanisms of the ‘safe’ (allowed) doses of MSG effect on a living organism.

scholarly journals IDENTIFICATION OF SOME CYTOCHEMICAL REACTION PRODUCTS BY HISTORADIOGRAPHY

1962 ◽  
Vol 15 (3) ◽  
pp. 417-425 ◽  
Author(s):  
Arthur J. Hale
Keyword(s):  
Acid Phosphatase ◽  
Carbonic Anhydrase ◽  
Succinate Dehydrogenase ◽  
Light Microscope ◽  
X Rays ◽  
Reaction Products ◽  
Cytochemical Reaction ◽  
Thiolacetic Acid

The reaction products of cytochemical methods for acid phosphatase, carbonic anhydrase succinate dehydrogenase, and thiolacetic acid esterase have been identified by historadiographic methods in sections of kidney. The absorption of x-rays by these products gives information on their localisation when they cannot be seen readily in the light microscope. The requirements for successful demonstration of such reaction products and the possibley value of the technique are discussed.

scholarly journals A HISTOCHEMICAL STUDY OF PHAGOCYTIC AND ENZYMATIC FUNCTIONS OF RABBIT MONONUCLEAR AND POLYMORPHONUCLEAR EXUDATE CELLS AND ALVEOLAR MACROPHAGES

1963 ◽  
Vol 17 (3) ◽  
pp. 465-486 ◽  
Author(s):  
Arthur M. Dannenberg ◽  
Marvin S. Burstone ◽  
Paul C. Walter ◽  
June W. Kinsley
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Alveolar Macrophages ◽  
Histochemical Study ◽  
Succinic Dehydrogenase ◽  
Enzymatic Activities ◽  
Dust Particles ◽  
Cell Type ◽  
Microbial Agents

The cytochrome oxidase (CO), aminopeptidase (AMP), succinic dehydrogenase (SD), acid phosphatase, esterase, and alkaline phosphatase of rabbit mononuclear (MN) and polymorphonuclear (PMN) peritoneal exudate cells and pulmonary alveolar macrophages (AM) - air dried on Mylar strips - were characterized by histochemical techniques with respect to stability, activators, inhibitors, and pH optima. A granule count method was established for the quantitation of these enzymes. For the acid phosphatase of MN, in which the most precise results were obtained, time, pH, substrate, and inhibitor curves resembled those commonly obtained biochemically. Five of these enzymes were usually more active in AM than MN, whereas the sixth, alkaline phosphatase, was not present in either cell type. AM also tended to consume more oxygen than MN and to divide more frequently. Since the most active cells in the population would be first involved in the host's defense against microbial agents, a comparison was made of the 10 per cent of the AM and MN with the highest enzymatic activities. No differences were found in the granule counts that were not reflected by the means. However, within a given AM population, cells containing ingested dust particles seemed to have higher enzymatic activities than those without particles. MN had greater acid phosphatase and SD activities than PMN and consumed more oxygen, but the CO, AMP, and esterase activites of both types of cells were of similar magnitude. PMN showed high alkaline phosphatase activity; MN showed none. A survey of the histochemical literature indicates that a positive correlation between the enzymatic and phagocytic activities of both MN and PMN exists in vivo.

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