Analysis of the genetic complexity of a hybrid plant

1978 ◽  
Vol 56 (10) ◽  
pp. 1291-1296
Author(s):  
Estela Sánchez de Jiménez ◽  
Alejandro Otto Meyer Willerer
Keyword(s):  
Repetitive Dna ◽  
Similar Pattern ◽  
Nuclear Dna ◽  
Hybrid Plant ◽  
Controlled Conditions ◽  
Three Samples ◽  
Genetic Complexity ◽  
Highly Repetitive Dna ◽  
Total Dna ◽  
Repetitive Fraction

Nuclear DNA from wheat, rye, and Triticale plantlets was isolated and purified. Purity of the samples was tested spectrophotometrically. Tm values and hyperchromicities were determined for the three DNA samples. The DNA(s) were denaturated and annealed under controlled conditions. Cot curves were carried out for total DNA on the three samples and for the repetitive DNA fractions.The analysis of those curves indicated that the genomes of wheat and rye are made of highly repetitive, intermediate, and unique DNA fractions. The highly repetitive fraction (107) is suggested as corresponding to palindrome areas in the genome.The Triticale genome showed a similar pattern to the parents, except for the highly repetitive DNA fraction which showed different orders of repetitiveness.

The molecular characterization of the genome of Muraena helena L. I. Isolation and hybridization of two MboI-restricted DNA fractions

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 809-813 ◽  
Author(s):  
G. Pichiri ◽  
M. Nieddu ◽  
R. Mezzanotte ◽  
P. P. Coni ◽  
S. Salvadori ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Organizer Region ◽  
Nucleolar Organizer Region ◽  
Electrophoretic Pattern ◽  
Nucleolar Organizer ◽  
Fish Chromosomes ◽  
Highly Repetitive Dna ◽  
Total Dna

To investigate the genome of the anguilliform fish Muraena helena at the molecular level we characterized total DNA by agarose gel electrophoresis after cleavage with AluI, HaeIII, MboI, and DdeI restriction endonucleases. Subsequently, we isolated the DNA from two specific electrophoretic fractions to be used as probes for Southern and in situ hybridization experiments. One such fraction showed an electrophoretic pattern typical of highly repetitive DNA localized in the centromeres of many chromosomes. The other fraction was shown to be located in the nucleolar organizer region, partially coincident with 45S rDNA, and to be composed of highly repetitive sequences.Key words: fish chromosomes, rDNA, highly repetitive DNA.

Meiotic behavior and DNA content in alloplasmic lines of maize

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 723-729 ◽  
Author(s):  
Lidia Poggio ◽  
Marcela Rosato ◽  
Carlos A. Naranjo
Keyword(s):  
Zea Mays ◽  
Dna Content ◽  
Nuclear Dna ◽  
Genomic Structure ◽  
Nuclear Dna Content ◽  
Meiotic Behavior ◽  
Maize Genotypes ◽  
Highly Repetitive Dna ◽  
Total Dna ◽  
And Function

The cytoplasm of Zea mays ssp. mexicana (teosinte) affects several inherited traits when combined with genotypes of maize (Zea mays ssp. mays). The meiotic behavior and the total DNA content of four lines of maize with teosinte cytoplasm were compared with those of the parental lines. The results obtained suggest that the cytoplasm of teosinte promotes an increase in total nuclear DNA content, perhaps through an increase of highly repetitive DNA in the knob zones. The analysis of meiotic behavior indicates that the cytoplasm of teosinte can alter the spatial distribution of the genomes, since two groups of five bivalents each were observed at a high frequency. During prophase I – anaphase I, each group of five bivalents behaves in a slightly asynchronous way with respect to the other group and, moreover, two nucleoli were observed in 10% of the cells. These results suggest that the cytoplasm of teosinte could induce changes affecting genomic structure and function in some maize genotypes. These changes are of potential importance for breeding programs and evolutionary studies.Key words: maize, teosinte, Zea mays ssp. mexicana, alloplasmy, nuclear-cytoplasmic interaction, meiotic behavior, DNA content.

A highly repetitive DNA family is dispersed in small clusters throughout the genome

1986 ◽  
Vol 10 (6) ◽  
pp. 486 ◽  
Author(s):  
R BATISTONI ◽  
R VIGNALI ◽  
A NEGRONI ◽  
F CREMISI ◽  
G BARSACCHIPILONE
Keyword(s):  
Repetitive Dna ◽  
Highly Repetitive Dna ◽  
Small Clusters

Sequence Organization of Simple, Highly Repetitive DNA Elements inBrassicaspecies

1991 ◽  
Vol 42 (2) ◽  
pp. 243-249 ◽  
Author(s):  
D. R SIBSON ◽  
S. G. HUGHES ◽  
J. A. BRYANT ◽  
P. N. FITCHETT
Keyword(s):  
Repetitive Dna ◽  
Sequence Organization ◽  
Dna Elements ◽  
Highly Repetitive Dna ◽  
Repetitive Dna Elements

Restriction Endonuclease Analysis of Highly Repetitive DNA as a Phylogenetic Tool

1997 ◽  
Vol 45 (3) ◽  
pp. 332-336 ◽  
Author(s):  
V.V. Grechko ◽  
L.V. Fedorova ◽  
A.N. Fedorov ◽  
S.Ya. Slobodyanyuk ◽  
D.M. Ryabinin ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Repetitive Dna ◽  
Restriction Endonuclease Analysis ◽  
Highly Repetitive Dna ◽  
Endonuclease Analysis

Satellite DNA in the ant Messor structor (Hymenoptera, Formicidae)

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 881-886 ◽  
Author(s):  
P Lorite ◽  
MF García ◽  
T Palomeque
Keyword(s):  
Key Words ◽  
Repetitive Dna ◽  
Tandem Repeat ◽  
Satellite Dna ◽  
Restriction Analysis ◽  
First Record ◽  
Satellite Dnas ◽  
Total Dna

This paper is the first record of the satellite DNA of Formicidae. The satellite DNA of the ant Messor structor is organized in a tandem repeat of monomers of 79 bp. Like satellite DNAs of other insects, it is AT rich and presents direct and inverted internal repeats. Restriction analysis of the total DNA with methylation-sensitive enzymes strongly suggests that this DNA is undermethylated. The presence of this repetitive DNA in other species of the genus Messor is also tested. Key words: Formicidae, methylation, nucleotide DNA composition, satellite DNA.

scholarly journals Functional role of a highly repetitive DNA sequence in anchorage of the mouse genome

1988 ◽  
Vol 16 (17) ◽  
pp. 8351-8360 ◽  
Author(s):  
Beatrice Neuer-Nitsche ◽  
Xiang Lu ◽  
Dieter Werner
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Functional Role ◽  
Mouse Genome ◽  
Repetitive Dna Sequence ◽  
Highly Repetitive Dna
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