Restriction Endonuclease Analysis of Highly Repetitive DNA as a Phylogenetic Tool

1997 ◽  
Vol 45 (3) ◽  
pp. 332-336 ◽  
Author(s):  
V.V. Grechko ◽  
L.V. Fedorova ◽  
A.N. Fedorov ◽  
S.Ya. Slobodyanyuk ◽  
D.M. Ryabinin ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Repetitive Dna ◽  
Restriction Endonuclease Analysis ◽  
Highly Repetitive Dna ◽  
Endonuclease Analysis

Parentage of Caucasian parthenogenetic rock lizard species (Lacerta) as revealed by restriction endonuclease analysis of highly repetitive DNA

1997 ◽  
Vol 18 (4) ◽  
pp. 407-418
Author(s):  
Ilya S. Darevsky ◽  
Vernata V. Grechkol ◽  
Dmitry M. Ryabinin ◽  
Larissa V. Fedorova ◽  
Alexey N. Fedorov ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Repetitive Dna ◽  
Restriction Endonuclease Analysis ◽  
Lizard Species ◽  
New Approach ◽  
Parthenogenetic Species ◽  
Rock Lizard ◽  
Highly Repetitive Dna ◽  
Species Specific ◽  
Hydrolysis Of

AbstractThe Caucasian parthenogenetic rock lizards of the L. saxicola complex probably originated by hybridisation of some bisexual species. We verified this hypothesis using a new approach based on comparison of repetitive DNA characters, which produce species-specific patterns (named "taxonprint"). The method relies on restriction endonuclease hydrolysis of genomic DNA, with the following 32P-end labeling and polyacrylamide gel electrophoretic separation of the fragments. The parthenogenetic species L. armeniaca and L. dahli possess specific features both of putative maternal L. mixta (in some taxonprints), and of paternal L. valentini (in other ones), whereas L. portschinskii and L. rudis also could be the paternal species. Parthenoclones L. unisexualis and L. uzzelli have the specific DNA features in several taxonprints of L. raddei or L. nairensis (which cannot be discriminated by taxonprints) which are supposed to be maternal species for L. unisexualis and paternal ones for L. uzzelli. The specific features of L. valentini (or L. portschinskii) are observed in L. unisexualis; L. uzzelli has not been investigated in this respect. Parthenogenetic L. rostombekovi also possesses properties of L. raddei or L. nairensis, but we have not be able to find any features of supposed paternal L. portschinskii in this parthenogenetic species.

scholarly journals Continuing Mycobacterium bovis transmission from animals to humans in New Zealand

2006 ◽  
Vol 134 (5) ◽  
pp. 1068-1073 ◽  
Author(s):  
M. G. BAKER ◽  
L. D. LOPEZ ◽  
M. C. CANNON ◽  
G. W. DE LISLE ◽  
D. M. COLLINS
Keyword(s):  
New Zealand ◽  
Restriction Endonuclease ◽  
Mycobacterium Bovis ◽  
Restriction Endonuclease Analysis ◽  
Domestic Animals ◽  
Human Infection ◽  
Potential Contribution ◽  
Large Reservoir ◽  
Animal Reservoirs ◽  
Endonuclease Analysis

New Zealand has a large reservoir of Mycobacterium bovis infection in wild and farmed animals. This study aimed to assess the extent of human infection with this organism and the potential contribution of these animal sources. Combined epidemiological and laboratory investigation of human tuberculosis cases over the period 1995–2002 showed that M. bovis accounted for 2·7% (54/1997) of laboratory-confirmed human tuberculosis cases, a rate of 0·2/100000 population. M. bovis isolates from humans (23) were typed using restriction endonuclease analysis (REA) and compared with isolates from wild and domestic animals (2600). Fourteen (61%) of the human isolates had REA patterns that were identical to patterns for isolates from cattle, deer, possums, ferrets, pigs, and occasionally cats. These results suggest a low level of ongoing M. bovis transmission from animal reservoirs to humans in New Zealand.

Transfusion transmission of cytomegalovirus confirmed by restriction endonuclease analysis

1985 ◽  
Vol 107 (6) ◽  
pp. 953-956 ◽  
Author(s):  
Mark D. Tolpin ◽  
John A. Stewart ◽  
Dora Warren ◽  
Benjamin A. Mojica ◽  
Mary A. Collins ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Restriction Endonuclease Analysis ◽  
Endonuclease Analysis
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