Assembly of particles from southern bean mosaic virus and sowbane mosaic virus components

1977 ◽  
Vol 55 (16) ◽  
pp. 2274-2277 ◽  
Author(s):  
J. H. Tremaine ◽  
W. P. Ronald
Keyword(s):  
Mosaic Virus ◽  
Dextran Sulfate ◽  
Ethylenediaminetetraacetic Acid ◽  
Sodium Dodecyl ◽  
Divalent Metal ◽  
Spherical Particles ◽  
Divalent Metal Ions ◽  
Southern Bean Mosaic Virus ◽  
Sodium Dextran Sulfate ◽  
Protein Components

Southern bean mosaic virus (SBMV) and sowbane mosaic virus (SoMV) were each dissociated into RNA and protein components in neutral pH buffers containing ethylenediaminetetraacetic acid and 1 M NaCl. The assembly of SBMV particles and of SoMV RNA in SBMV protein was accomplished by dialysis of the virus components into low molarity buffers containing divalent metal ions. Some of these particles were stable in 1% sodium dodecyl sulfate (SDS) and had sedimentation and electrophoretic properties identical with untreated virus. Spherical particles were also assembled with either SBMV or SoMV RNA in SoMV protein and with sodium dextran sulfate in either SBMV or SoMV protein, but these particles were not stable in 1% SDS.

Comparison of highly basic cyanogen bromide peptides from strains of southern bean mosaic virus

1981 ◽  
Vol 27 (7) ◽  
pp. 654-663 ◽  
Author(s):  
J. H. Tremaine ◽  
W. P. Ronald ◽  
E. M. Kelly
Keyword(s):  
Mosaic Virus ◽  
Cyanogen Bromide ◽  
Ethylenediaminetetraacetic Acid ◽  
Exchange Chromatography ◽  
Spherical Particles ◽  
Partial Identity ◽  
Sodium Phosphate Buffer ◽  
Southern Bean Mosaic Virus ◽  
Cross Absorption ◽  
Viruslike Particles

Electrophoresis patterns of cyanogen bromide (CNBr) peptides from the proteins of the cowpea (SBc), bean (SBb), Mexican (SBm), and Ghana (SBg) strains of southern bean mosaic virus showed a marked similarity between SBc and SBg and between SBb and SBm. A highly basic CNBr peptide from SBc, cCB-1, was isolated by Sephadex and ion-exchange chromatography. The size and amino acid composition of cCB-1 was similar but differed from that of the N-terminal CNBr peptide bCB-1 from SBb described previously.Antisera to cCB-1 or bCB-1 conjugated to tomato bushy stunt virus (TBSV) were used in gel diffusion tests with virus particles of the four SB strains. With the bCB-1–TBSV conjugate antiserum, SBb gave a reaction of identity with SBm and reactions of partial identity with SBc and SBg. With the cCB-1–TBSV conjugate antiserum, SBc gave a reaction of identity with SBg and reactions of partial identity with SBb and SBm. Cross absorption of bCB-1–TBSV conjugate antiserum with TBSV yielded an antiserum that reacted with SBb but not with TBSV. Cross absorption with bCB-1 yielded an antiserum that reacted with TBSV but not with SBb.Limited tryptic proteolysis of SBc in 0.01 M sodium phosphate buffer containing 0.01 M ethylenediaminetetraacetic acid, pH 8.0, resulted in the formation of viruslike particles and smaller spherical particles. Sixteen peptides were isolated from these digests and the compositions of most of them were similar to but not identical to peptides isolated from limited tryptic proteolysis of SBb.

Preparation of optically clear lyophilized human serum for use in preparing control material.

1976 ◽  
Vol 22 (4) ◽  
pp. 456-460 ◽  
Author(s):  
G J Proksch ◽  
D P Bonderman
Keyword(s):  
Human Serum ◽  
Dextran Sulfate ◽  
Raw Materials ◽  
Divalent Metal ◽  
Divalent Metal Ions ◽  
Quality Control Material ◽  
Control Material ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Citrate Phosphate

Abstract We describe a simple, rapid process--which includes the specific precipitation of pre-beta and beta-lipoproteins with dextran sulfate and divalent metal ions--for preparing an optically clear human serum that retains its clarity upon reconstitution with water after having been frozen and lyophilized. Such serum contains the normal constituents of human serum, except for the removed lipoproteins. The process causes no apparent interference with results of analyses for 22 of the more commonly measured constituents. Fresh or aged pooled serum or blood-bank plasma containing acid-citrate-dextrose or citrate-phosphate-dextrose are equally suitable as raw materials. This stabilized serum is an excellent matrix for use in preparing standards and quality-control material for assay of components of human serum.

scholarly journals First Report of Southern bean mosaic virus Infecting French Bean in Morocco

Plant Disease ◽  
2004 ◽  
Vol 88 (10) ◽  
pp. 1162-1162 ◽  
Author(s):  
E. Segundo ◽  
F. M. Gil-Salas ◽  
D. Janssen ◽  
G. Martin ◽  
I. M. Cuadrado ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Sodium Dodecyl ◽  
Plant Viruses ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Sequence Identity ◽  
Southern Bean Mosaic Virus ◽  
Bean Plants ◽  
Symptomatic Leaf ◽  
Das Elisa

Common bean (Phaseolus vulgaris L.) is grown on approximately 1,500 ha in commercial greenhouses and is of major economic importance in the Souss-Massa Region, Agadir, Morocco. Since October 2003, symptoms resembling a viral disease, consisting of pod mosaic and distortion and mild to severe mosaic in leaves, have been observed on bean plants in several greenhouses. Mechanical inoculation with symptomatic leaf extracts produced necrotic local lesions on P. vulgaris ‘Pinto’ and systemic symptoms similar to those observed in the naturally infected bean plants P. vulgaris ‘Donna’ (five plants per cultivar). Inoculated and naturally infected samples reacted positively using a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) to Southern bean mosaic virus (SBMV) (DSMZ, Braunschweig, Germany), a member of the Sobemovirus genus that is transmitted by contact, soil, beetles, and seeds (1). Virions purified from a naturally infected ‘Donna’ plant contained a 30-kDa polypeptide that reacted positively using sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot analysis with SBMV antiserum (DSMZ). Reverse transcription-polymerase chain reaction amplification with SMBV primers as described by Verhoeven et al. (2) produced an expected 870-bp band. The amplicon was cloned, sequenced (GenBank Accession No. AJ748276), and compared to those isolates available in GenBank and had a nucleotide sequence identity of 87% and a derived amino acid sequence identity of 95% with an SBMV isolate from Spain (2). During a survey in different areas of the Souss-Massa Region, 20 symptomatic leaf and pod samples were randomly collected from 12 greenhouses (50 ha) where significant commercial losses were suffered because of this virus disease, and all samples were positive using DAS-ELISA for SBMV. To our knowledge, this is the first report of SBMV in Morocco. References: (1) J. H. Tremaine and R. I. Hamilton. Southern bean mosaic virus. No. 274 in: Descriptions of Plant Viruses. CMI/AAB, Kew, Surrey, England, 1983. (2) J. Th. J. Verhoeven et al. Eur. J. Plant Pathol. 109:935, 2003.

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