Histopathology and histochemistry of sugar beet seedlings resistant and susceptible to Aphanomyces cochlioides

1975 ◽  
Vol 53 (3) ◽  
pp. 284-294 ◽  
Author(s):  
Leonard J. Herr
Keyword(s):  
Cell Wall ◽  
Sugar Beet ◽  
Cortical Tissue ◽  
Cortical Cells ◽  
Aphanomyces Cochlioides ◽  
Histochemical Methods ◽  
Resistant Varieties

Hyphae were observed within diseased cortical tissue of hypocotyl lesions of both the susceptible and the resistant varieties but were not detected in symptomless tissues in advance of lesion margins. Hyphae ramified extensively intercellularly and, to a lesser extent, intracellularly. Only in severely diseased beets were hyphae found within the stele. Protoplasts of cortical cells in proximity to hyphae appeared to lose turgor early in pathogenesis; then the cells collapsed and cortical tissue was apparently macerated. Histochemically, cell wall constituents (pectin, hemicellulose, non-cellulosic polysaccharides, lignin, suberin, and cutin) were little changed in diseased beets as compared with healthy beets. Peroxidase and (at times) phenolase activity increased and phenols and quinones accumulated in the stele of diseased plants (adjacent to lesions) to levels above those found in healthy plants. Possibly these phenols and quinones may inhibit colonization of stelar tissues. No differences in accumulation or localization of these substances in the resistant as compared with the susceptible beet varieties were detected by histochemical methods.

scholarly journals Transcriptomic Analysis of Betula halophila in Response to Salt Stress

2018 ◽  
Vol 19 (11) ◽  
pp. 3412 ◽  
Author(s):  
Fenjuan Shao ◽  
Lisha Zhang ◽  
Iain Wilson ◽  
Deyou Qiu
Keyword(s):  
Cell Wall ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Expression Patterns ◽  
Soil Salinization ◽  
Sphingolipid Metabolism ◽  
Sequencing Platform ◽  
Resistant Varieties ◽  
High Salt Tolerance ◽  
Salt Overly Sensitive

Soil salinization is a matter of concern worldwide. It can eventually lead to the desertification of land and severely damage local agricultural production and the ecological environment. Betula halophila is a tree with high salt tolerance, so it is of importance to understand and discover the salt responsive genes of B. halophila for breeding salinity resistant varieties of trees. However, there is no report on the transcriptome in response to salt stress in B. halophila. Using Illumina sequencing platform, approximately 460 M raw reads were generated and assembled into 117,091 unigenes. Among these unigenes, 64,551 unigenes (55.12%) were annotated with gene descriptions, while the other 44.88% were unknown. 168 up-regulated genes and 351 down-regulated genes were identified, respectively. These Differentially Expressed Genes (DEGs) involved in multiple pathways including the Salt Overly Sensitive (SOS) pathway, ion transport and uptake, antioxidant enzyme, ABA signal pathway and so on. The gene ontology (GO) enrichments suggested that the DEGs were mainly involved in a plant-type cell wall organization biological process, cell wall cellular component, and structural constituent of cell wall molecular function. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment showed that the top-four enriched pathways were ‘Fatty acid elongation’, ‘Ribosome’, ‘Sphingolipid metabolism’ and ‘Flavonoid biosynthesis’. The expression patterns of sixteen DEGs were analyzed by qRT-PCR to verify the RNA-seq data. Among them, the transcription factor AT-Hook Motif Nuclear Localized gene and dehydrins might play an important role in response to salt stress in B. halophila. Our results provide an important gene resource to breed salt tolerant plants and useful information for further elucidation of the molecular mechanism of salt tolerance in B. halophila.

scholarly journals Changes in cell ultrastructure and morphology of Arabidopsis thaliana roots after coumarins treatment

2014 ◽  
Vol 70 (3) ◽  
pp. 187-198
Author(s):  
Ewa Kupidłowska
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Walls ◽  
Root Elongation ◽  
Inhibitory Effect ◽  
Cell Ultrastructure ◽  
Radial Expansion ◽  
Elongation Zone ◽  
Cortical Cells ◽  
Mother Cell Wall

The ultrastructure and morphology of roots treated with coumarin and umbelliferone as well as the reversibility of the coumarins effects caused by exogenous GA, were studied in <em>Arabidopsis thaliana</em>. Both coumarins suppressed root elongation and appreciably stimulated radial expansion of epidermal and cortical cells in the upper part of the meristem and in the elongation zone. The gibberellic acid applied simultaneously with coumarins decreased their inhibitory effect on root elongation and reduced cells swelling.Microscopic observation showed intensive vacuolization of cells and abnormalities in the structure of the Golgi stacks and the nuclear envelope. The detection of active acid phosphatase in the cytosol of swollen cells indicated increased membrane permeability. Significant abnormalities of newly formed cell walls, e.g. the discontinuity of cellulose layer, uncorrect position of walls and the lack of their bonds with the mother cell wall suggest that coumarins affected the cytoskeleton.

Developmental changes in cell-wall ferulate and dehydrodiferulates in sugar beet

1999 ◽  
Vol 52 (5) ◽  
pp. 819-827 ◽  
Author(s):  
Gundolf Wende ◽  
Keith W Waldron ◽  
Andrew C Smith ◽  
Christopher T Brett
Keyword(s):  
Cell Wall ◽  
Sugar Beet ◽  
Developmental Changes

Characteristics of a disease of Sphagnum fuscum caused by Scleroconidioma sphagnicola

2001 ◽  
Vol 79 (10) ◽  
pp. 1217-1224 ◽  
Author(s):  
A Tsuneda ◽  
M H Chen ◽  
R S Currah
Keyword(s):  
Cell Wall ◽  
Fungal Pathogen ◽  
Infected Leaf ◽  
Disease Development ◽  
Cortical Cells ◽  
Necrotrophic Pathogen ◽  
Sphagnum Fuscum ◽  
Entire Plant ◽  
Host Cell Wall ◽  
Advanced Stages

Scleroconidioma sphagnicola Tsuneda, Currah & Thormann, a dematiaceous hyphomycetous fungus, was found to cause a disease of Sphagnum fuscum (Schimp.) Klinggr. Hyphae of S. sphagnicola penetrated into chlorophyllose cells of host leaves and caused degeneration of chloroplasts, resulting in chlorosis of the infected leaves. Parasite hyphae often grew inside the host cell wall, and cavities were created around the hyphae. The invaded cell wall of Sp. fuscum appeared swollen and showed wavy deformation. In advanced stages of disease development, infected leaf chlorophyllose cells and stem cortical cells were necrotic and the entire plant became brown, shriveled, and brittle. Hyphae of S. sphagnicola began to form microsclerotia during early stages of disease development. Microsclerotia either formed conidiogenous cells on their surface or remained metabolically inactive and did not form conidiogenous cells. It was concluded that S. sphagnicola is a necrotrophic pathogen of Sp. fuscum, and its morphological and phenological features show remarkable adaptations for dispersal and colonization as a pathogen in bog habitats.Key words: moss, fungal pathogen, pathogenesis, chlorosis, necrosis, microsclerotia.

Aphanomyces cochlioides. [Distribution map].

2021 ◽  
Author(s):  
Keyword(s):  
Nova Scotia ◽  
North America ◽  
South America ◽  
Sugar Beet ◽  
South Dakota ◽  
Geographical Distribution ◽  
Spinacia Oleracea ◽  
Distribution Map ◽  
Aphanomyces Cochlioides ◽  
New Distribution

Abstract A new distribution map is provided for Aphanomyces cochlioides Drechsler. Peronosporea: Saprolegniales: Leptolegniaceae. Hosts: spinach (Spinacia oleracea), sugar beet (Beta vulgaris), and other members of the Chenopodiaceae and Amaranthaceae. Information is given on the geographical distribution in Africa (Egypt), Asia (Japan, Hokkaido, Turkey), Europe (Austria, Belgium, Bulgaria, Croatia, Denmark, Estonia, France, Germany, Hungary, Ireland, Moldova, Netherlands, Poland, Russia, Spain, Sweden, Ukraine, UK), North America (Canada, Alberta, Nova Scotia, Ontario, Quebec, USA, Arizona, California, Connecticut, Idaho, Indiana, Iowa, Maine, Michigan, Minnesota, Montana, Nebraska, North Dakota, Ohio, South Dakota, Texas, Washington, Wisconsin, Wyoming), Oceania (Australia, Queensland), and South America (Chile).

Secondary Metabolite- and Endochitinase-Dependent Antagonism toward Plant-Pathogenic Microfungi of Pseudomonas fluorescens Isolates from Sugar Beet Rhizosphere

1998 ◽  
Vol 64 (10) ◽  
pp. 3563-3569 ◽  
Author(s):  
Mette Neiendam Nielsen ◽  
Jan Sørensen ◽  
Johannes Fels ◽  
Hans Christian Pedersen
Keyword(s):  
Biological Control ◽  
Cell Wall ◽  
Sugar Beet ◽  
Pseudomonas Fluorescens ◽  
Secondary Metabolite ◽  
Pythium Ultimum ◽  
Medium Composition ◽  
Fungal Antagonism ◽  
Lipopeptide Antibiotic

ABSTRACT Forty-seven isolates representing all biovars of Pseudomonas fluorescens (biovars I to VI) were collected from the rhizosphere of field-grown sugar beet plants to select candidate strains for biological control of preemergence damping-off disease. The isolates were tested for in vitro antagonism toward the plant-pathogenic microfungi Pythium ultimum and Rhizoctonia solani in three different plate test media. Mechanisms of fungal inhibition were elucidated by tracing secondary-metabolite production and cell wall-degrading enzyme activity in the same media. Most biovars expressed a specific mechanism of antagonism, as represented by a unique antibiotic or enzyme production in the media. A lipopeptide antibiotic, viscosinamide, was produced independently of medium composition by P. fluorescens bv. I, whereas the antibiotic 2,4-diacetylphloroglucinol was observed only in glucose-rich medium and only in P. fluorescens bv. II/IV. Both pathogens were inhibited by the two antibiotics. Finally, in low-glucose medium, a cell wall-degrading endochitinase activity in P. fluorescens bv. I, III, and VI was the apparent mechanism of antagonism toward R. solani. The viscosinamide-producing DR54 isolate (bv. I) was shown to be an effective candidate for biological control, as tested in a pot experiment with sugar beet seedlings infested with Pythium ultimum. The assignment of different patterns of fungal antagonism to the biovars of P. fluorescens is discussed in relation to an improved selection protocol for candidate strains to be used in biological control.

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