Growth of Aphanomyces cochlioides in synthetic media as affected by carbon, nitrogen, methionine, and trace elements

1973 ◽  
Vol 51 (12) ◽  
pp. 2495-2503 ◽  
Author(s):  
Leonard J. Herr
Keyword(s):  
Trace Elements ◽  
Glutamic Acid ◽  
Synthetic Medium ◽  
Maximum Yield ◽  
Major Elements ◽  
Aphanomyces Cochlioides ◽  
N Source ◽  
Trace Element Nutrition ◽  
Methionine Concentration ◽  
Synthetic Media

Aspects of the nutrition of Aphanomyces cochlioides were investigated using a synthetic medium containing the major elements (ME) C, N, S, Ca, K, Mg, P; with or without the trace elements (TE) Fe, Zn, Cu, B, Mn, Mo. Primary C, N, and S sources were D-glucose, L-asparagine or DL-glutamic acid, and DL-methionine. In the presence or absence of individual trace elements, growth increased with Fe, decreased with Cu, and was unaffected by the remaining trace elements. Combining Fe with Cu eliminated the toxicity of Cu. C, N, and methionine concentration level effects were studied in relation to trace element nutrition and N sources in a series of factorial experiments. Trace elements strongly affected growth (maximum yield minus TE = 21.0 mg; maximum yield plus TE = 139.7 mg). As a source of C, glucose supported greater growth (83 mg) than sucrose (18 mg), while growth without added C was poor (7 mg). L-Asparagine was superior to DL-glutamic acid as a source of N at high C levels and the effect of methionine concentrations on growth differed with the N source. D-Asparagine was not utilized as a source of N. Growth in the synthetic medium approached that obtained in a complex medium.

scholarly journals Use of Nonspecific, Glutamic Acid-Free, Media and High Glycerol or High Amylase as Inducing Parameters for Screening Bacillus Isolates Having High Yield of Polyglutamic Acid

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Nandita N. Baxi
Keyword(s):  
Glutamic Acid ◽  
Synthetic Medium ◽  
Maximum Yield ◽  
Proteinase K ◽  
High Yield ◽  
Polyglutamic Acid ◽  
Production Medium ◽  
Coomassie Blue ◽  
Sds Page ◽  
Free Media

Out of fifty-five Bacillus isolates obtained from ten different regional locations and sources, seven showed the ability to consistently produce specific extracellular polymeric substance (EPS) on rich as well as synthetic but nonspecific media which did not contain glutamic acid. The isolates were identified as either Bacillus licheniformis or Bacillus subtilis. The EPS from all isolates was resistant to alpha protease, proteinase K, and was thus of high molecular weight. Further it was detected after SDS-PAGE by methylene blue but not by coomassie blue R staining as in case of proteins with high proportion of acidic amino acids. Cell-free EPS, after acid hydrolysis, showed absence of carbohydrates and presence of only glutamic acid. Thus the native the EPS from all seven isolates was confirmed to be gamma polyglutamic acid (PGA) and not exopolysaccharide. The Bacillus isolate T which produced maximum polymer on all media tested had higher amylase: protease activity as compared to other strains. If inoculum was developed in rich medium as compared to synthetic medium, the PGA produced increased by twofold in the subsequent synthetic production medium. Similarly, use of inoculum consisting of young and vegetative cells also increased the PGA production by twofold though amount of inoculum did not affect yield of PGA. Though PGA was produced in even in the absence of glutamic acid supplementation in the production medium by all isolates, the yield of PGA increased by fourfold in the presence glutamic acid and the maximum yield was 30 g/l for isolate K. The supplementation of glutamine instead of glutamic acid into the medium caused an increase in the viscosity of the non-Newtonian solution of PGA.

Nutritional requirements of some non-pathogenic Neisseria grown in simple synthetic media

1975 ◽  
Vol 21 (8) ◽  
pp. 1198-1204 ◽  
Author(s):  
I. J. McDonald ◽  
K. G. Johnson
Keyword(s):  
Amino Acids ◽  
Nicotinic Acid ◽  
Glutamic Acid ◽  
Ferrous Sulfate ◽  
Synthetic Medium ◽  
Potassium Phosphate ◽  
Calcium Pantothenate ◽  
Liquid Media ◽  
Carbon And Nitrogen ◽  
Synthetic Media

Ten species of non-pathogenic Neisseria were grown in simple defined liquid media containing amino acids, biotin, nicotinic acid, calcium pantothenate, ferrous sulfate, magnesium sulfate, and potassium phosphate. Two of these Neisseria were induced to grow with glutamic acid as the carbon and nitrogen source. The remaining eight Neisseria grew in glutamic acid medium supplemented with from one to four additional amino acids, lactate, or lactate and glucose. A strain of N. flavescens grew in the absence of added growth factors whereas the remaining nine species of Neisseria required either biotin or nicotinic acid; pantothenate was required by two and was stimulatory for three of these species. Use of carbohydrates by the non-pathogenic Neisseria in synthetic medium was tested. Two strains failed to use any of the 14 carbohydrates tested; one strain used only glucose; the remaining seven strains used fructose, glucose, maltose, and sucrose to varying degrees.

THE METABOLISM OF ANIMAL TISSUES CULTIVATED IN VITRO: III. AMINO ACID METABOLISM OF STRAIN L CELLS IN COMPLETELY SYNTHETIC MEDIA

1958 ◽  
Vol 36 (1) ◽  
pp. 771-782 ◽  
Author(s):  
Arthur E. Pasieka ◽  
Helen J. Morton ◽  
Joseph F. Morgan
Keyword(s):  
Amino Acid ◽  
Glutamic Acid ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Amino Acid Content ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
L Cells ◽  
Synthetic Media

Strain L cells, of mouse fibroblastic origin, have been cultivated in vitro in completely synthetic medium M 150 and in various modifications of this medium. The amino acid changes in the nutrient medium during cell cultivation have been studied by paper chromatography. A characteristic pattern of amino acid uptake and accumulation in the medium has been found. No change in the alanine concentration was observed but omission of alanine from the culture medium resulted in its accumulation in appreciable amounts. Omission of glutamic acid did not alter the pattern of amino acid changes by the cells. Omission of glutamine increased the uptake of amino acids and prevented amino acid accumulation. Omission of both glutamic acid and glutamine resulted in a virtual cessation of amino acid changes in the culture medium. Strain L cells decreased the adenine content of the medium and produced small amounts of hypoxanthine. These changes were not affected by alterations in the amino acid content of the medium. Omission of glutamic acid and glutamine from the culture medium did not cause an appreciable decrease in cell population or apparent degeneration of the cultures over a 30-day period.

THE METABOLISM OF ANIMAL TISSUES CULTIVATED IN VITRO: III. AMINO ACID METABOLISM OF STRAIN L CELLS IN COMPLETELY SYNTHETIC MEDIA

1958 ◽  
Vol 36 (7) ◽  
pp. 771-782 ◽  
Author(s):  
Arthur E. Pasieka ◽  
Helen J. Morton ◽  
Joseph F. Morgan
Keyword(s):  
Amino Acid ◽  
Glutamic Acid ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Amino Acid Content ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
L Cells ◽  
Synthetic Media

Strain L cells, of mouse fibroblastic origin, have been cultivated in vitro in completely synthetic medium M 150 and in various modifications of this medium. The amino acid changes in the nutrient medium during cell cultivation have been studied by paper chromatography. A characteristic pattern of amino acid uptake and accumulation in the medium has been found. No change in the alanine concentration was observed but omission of alanine from the culture medium resulted in its accumulation in appreciable amounts. Omission of glutamic acid did not alter the pattern of amino acid changes by the cells. Omission of glutamine increased the uptake of amino acids and prevented amino acid accumulation. Omission of both glutamic acid and glutamine resulted in a virtual cessation of amino acid changes in the culture medium. Strain L cells decreased the adenine content of the medium and produced small amounts of hypoxanthine. These changes were not affected by alterations in the amino acid content of the medium. Omission of glutamic acid and glutamine from the culture medium did not cause an appreciable decrease in cell population or apparent degeneration of the cultures over a 30-day period.

Hydrochemistry and carbonate sediment characterisation of Bacalar Lagoon, Mexican Caribbean

2019 ◽  
Vol 70 (3) ◽  
pp. 382 ◽  
Author(s):  
Nidia I. Tobón Velázquez ◽  
Mario Rebolledo Vieyra ◽  
Adina Paytan ◽  
Kyle H. Broach ◽  
Laura M. Hernández Terrones
Keyword(s):  
Trace Elements ◽  
Water Column ◽  
Saturation Index ◽  
Major Elements ◽  
Anthropogenic Sources ◽  
Pollution Indices ◽  
Mexican Caribbean ◽  
High Concentrations ◽  
Geochemical Analyses ◽  
Changes Over Time

The aim of the study is to determine the distribution of trace and major elements in the water and in the sediments of the south part of the Bacalar Lagoon and to identify the sources of the trace elements and their changes over time. The western part of the lagoon water column is characterised by high concentrations of Ca2+, HCO3– and Sr2+, derived from groundwater input. In contrast, the eastern part of the lagoon is characterised by high concentrations of Mg2+, Na+ and Cl–. The lagoon is not affected by present-day seawater intrusion. Water column and sediment geochemical analyses performed in Bacalar Lagoon show clear spatial distribution of different parameters. The saturation index of the water column indicates three main groups: (1) a zone oversaturated with regard to aragonite, calcite and dolomite; (2) an undersaturated area where all three minerals are dissolving; and (3) an area with calcite equilibrium and undersaturation with regard to the other minerals. Herein we present the first measurements of trace element (Ba2+, Mn2+, K+, Ni2+, Zn2+) concentrations in carbonates obtained from sediments in Bacalar Lagoon. In order to evaluate whether the trace elements are derived from natural or anthropogenic sources, four pollution indices were calculated. The results confirmed that Bacalar Lagoon sediments are not contaminated with Ni2+, K+, Mn2+ and Ba2+, and that the Zn2+ seems to have a predominantly anthropogenic origin.

A study of the composition of Clun Forest ewe's milk. II. Mineral constituents

1966 ◽  
Vol 67 (1) ◽  
pp. 77-80 ◽  
Author(s):  
W. M. Ashton ◽  
I. M. Yousef
Keyword(s):  
Trace Elements ◽  
Statistical Analysis ◽  
Significant Variation ◽  
Mineral Content ◽  
Major Elements ◽  
Lactation Period ◽  
Average Composition ◽  
Sodium Potassium ◽  
Ewe's Milk ◽  
Mineral Constituents

1. This paper provides further information on the mineral content of the milk of Clun Forest ewes, particularly those elements not dealt with in a previous paper, namely, magnesium, sodium, potassium, and certain trace elements.2. The average composition was as follows: calcium 0.200, magnesium 0.0147, sodium 0.046, potassium 0.168, phosphorus 0.140 and chlorine 0.076%; copper 0.22, iron 0.77, manganese 0.07 and aluminium 1.7 parts per million. Where possible the results are compared with those obtained by other workers for ewe's and cow's milk.3. Statistical analysis of the data showed that, with the exception of phosphorus, the content of major elements varied significantly during the lactation period. There was also a significant variation in all major elements between sheep.

Current issues in trace element nutrition of grazing livestock in Australia and New Zealand

1999 ◽  
Vol 50 (8) ◽  
pp. 1341 ◽  
Author(s):  
D. G. Masters ◽  
G. J. Judson ◽  
C. L. White ◽  
J. Lee ◽  
N. D. Grace
Keyword(s):  
Trace Elements ◽  
New Zealand ◽  
Trace Element ◽  
Recent Work ◽  
Cost Effective ◽  
Trace Element Composition ◽  
Scientific Data ◽  
Pastoral System ◽  
Trace Element Nutrition ◽  
Grazing Livestock

Improving trace element nutrition of grazing animals, in a way that is cost effective and that meets consumer perceptions and preferences, is a continuing challenge. This review focuses on research over the past 10 years, addressing issues and perspectives on the roles, risks of inadequacy, and supplementary remedies of key trace elements, both essential and deleterious, which have an impact on the productivity and product quality of grazing livestock throughout Australia and New Zealand. The emphasis is on copper (Cu), cobalt (Co), iodine (I), selenium (Se), and, to a lesser extent, zinc (Zn) — the trace elements most frequently found to be deficient in parts of Australia and New Zealand. Research continues to elucidate new roles for trace elements in the animal, and as this leads to a better understanding of requirements, diagnostic criteria and supplementation strategies need re-evaluation. Newer perspectives on marginal deficiency for Cu, I, Se, and Zn are given and issues for risk management discussed. Advances in sustained delivery of trace elements such as Co (as vitamin B12), I, and Se are reported. The diagnosis and management of marginal Cu deficiency continue to be difficult, especially in New Zealand where recent work has shown that dietary antagonists [iron (Fe), molybdenum (Mo), and sulfur (S)] may impair Cu absorption at lower intakes than previously thought. There is still a dearth of scientific data on the advantages of using so-called organic trace element supplements (metals complexed or chelated with amino acids or peptides). Nevertheless their usage continues to increase. There is increasing evidence that trace elements influence the pathophysiology arising from the ingestion of toxins. This review summarises recent work on the role of Co in annual ryegrass toxicity; Cu, Se, and Zn in lupinosis; Cu and alkaloid toxicity; Zn and facial eczema; and Co and white liver disease. Trace elements are required to support immune function (e.g. as imposed parasite infection) and marginal deficiencies may be exacerbated by an immunological challenge. The roles of Cu, Co, Mo, Se, and, to a lesser extent, chromium and Zn have attracted attention and under conditions of stress there may be an additional need for these elements. Diversification in farming has led to the introduction of species such as deer (Cervus), alpacas (Camilids), emus, and ostriches (Ratites) and the paucity of information on trace element requirements for these species, and also for horses, indicates the need for further work. The effect of supplementation on trace element composition of meat, milk, and wool is also reviewed, both in terms of product characteristics and human health. Of the deleterious elements, cadmium has attracted the most interest and concern because of its introduction into the pastoral system from phosphate-based fertilisers.

Geochemistry and petrogenesis of the early Proterozoic Hemlock volcanic rocks and the Kiernan sills, southern Lake Superior region

1988 ◽  
Vol 25 (4) ◽  
pp. 528-546 ◽  
Author(s):  
W. C. Ueng ◽  
T. P. Fox ◽  
D. K. Larue ◽  
J. T. Wilband
Keyword(s):  
Trace Elements ◽  
Rare Earth ◽  
North Atlantic ◽  
North Atlantic Ocean ◽  
Lake Superior ◽  
Volcanic Rocks ◽  
Wall Rock ◽  
Major Elements ◽  
Chemical Contamination ◽  
Early Proterozoic

During the early Proterozoic, the 2 km thick differentiated gabbroic Kiernan sills were emplaced into a thick accumulation of pillow basalt and associated deep-water strata, the Hemlock Formation, in the southern Lake Superior region. On the basis of major elements and trace elements (including rare-earth-element data), the Kiernan sills and the hosting volcanic rocks of the Hemlock Formation were determined to be comagmatic in origin, and both evolved from assimilation – crystal fractionation processes. The major assimilated components in these igneous rocks are identified as terrigenous sedimentary rocks. Assimilation affected the abundance of Nb, Ta, light rare-earth elements, and most likely P, Rb, Th, and K in the magma. The effect of chemical contamination from wall-rock assimilation accumulates with increasing differentiation.With wall-rock contamination carefully evaluated, a series of tectonic discriminating methods utilizing immobile trace elements indicates that the source magma was a high-Ti tholeiitic basalt similar to present-day mid-ocean-ridge basalts (MORB). It is suggested from this study that most of the enriched large-ion lithophile elements and LREE of the magma were not inherited from the mantle but from assimilation of supracrustal rocks. Chemical signatures of these rocks are distinctively different from those of arc-related volcanics. A rifting tectonic regime analogous to the opening of the North Atlantic Ocean and extrusion of North Atlantic Tertiary volcanics best fits the criteria revealed by this study.

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