Effect of changes in carbohydrate concentration on the rate of net photosynthesis in mature leaves of Abies balsamea
The hypothesis that photosynthate accumulation within a leaf inhibits the ongoing rate of photosynthesis was investigated in 6-year-old balsam fir trees. Rates of net photosynthesis and transpiration, and concentrations of starch, total sugar, chlorophyll, and water were measured in 1-year-old or fully expanded, current needles, and leaf resistances to carbon dioxide transfer were calculated. The several-fold rise and fall in the content of carbohydrate (mostly starch) that occurs naturally in 1-year-old needles before and after budbreak was correlated positively rather than negatively with the photosynthetic rate. Shading during the normal period of starch accumulation decreased the concentrations of both starch and total sugar, but did not increase photosynthesis. Continuous cooling of the base of a branch to 2 °C to inhibit translocation of photosynthate did not affect the photosynthetic rate during a 2-week period, nor did it cause carbohydrate accumulation, as measured at the end of the experiment. Removing a ring of bark from a branch base resulted in a large increase in the foliar concentration of starch (but not total sugar) and a small decrease in photosynthesis. However, the concomitant decrease in moisture content, not the accumulation of starch, was more probably the cause of the reduced photosynthesis. We conclude that the rate of photosynthesis in fir needles, at least under normal growing conditions, is not influenced by photosynthate accumulation.