Influence of calcium on the metabolism of chlorophyll, carotene, nucleic acid, and protein in Scenedesmus

1973 ◽  
Vol 51 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Gaurangakumar Das
Keyword(s):  
Nucleic Acid ◽  
Protein Content ◽  
Dna Content ◽  
Culture Medium ◽  
Growth Period ◽  
Dry Weight ◽  
Weight Basis ◽  
Carotene Content ◽  
Calcium Supply

The influence of calcium supply on the chlorophyll, carotene, nucleic acid, and protein content in Scenedesmus obtusiusculus Chod. was investigated. Evidence showed that upon addition of calcium-starved cells to a culture medium containing calcium, the levels of all these components increased slowly for 15 h. During the next 33 h chlorophyll and carotene content increased more rapidly, and the chlorophyll a:b ratio also increased. During this growth period, both RNA and protein content increased linearly whereas DNA content did not increase on a dry weight basis throughout the period of observation.

Possible mechanism of gibberellin-induced chlorosis in lettuce seedlings

1973 ◽  
Vol 51 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Gaurangakumar Das
Keyword(s):  
Nucleic Acid ◽  
Protein Content ◽  
Sugar Content ◽  
Dry Weight ◽  
Weight Basis ◽  
Nucleic Acid Content ◽  
Chlorophyll B ◽  
Lactuca Sativa L ◽  
Grand Rapids ◽  
Rna And Dna

Effects of gibberellin A3 (GA) on growth, pigments, protein, and nucleic acid content of the cotyledons of lettuce seedlings (Lactuca sativa L. cv. Grand Rapids) are reported. GA treatment increased both the total shoot and cotyledon fresh weights, and decreased chlorophyll content per cotyledon and on a dry weight basis. GA also decreased the protein content of the cotyledon, but increased the level of RNA and DNA measured on a dry weight basis. The hormone had no effect on the pheophytin: chlorophyll ratio, but increased the ratios of chlorophyll a: chlorophyll b, phytol: chlorophyll, and carotene: chlorophyll. GA also increased the sugar content of the cotyledons. Treatment of excised cotyledons with glucose had an effect similar to that of GA on chlorophyll and protein content. The data are discussed in relation to the mechanism of GA-induced chlorosis.

Distribution of amylase and chymotrypsin in four regions of the rat pancreas

1981 ◽  
Vol 59 (8) ◽  
pp. 884-886 ◽  
Author(s):  
Adrien R. Beaudoin ◽  
Pierre St-Jean ◽  
Ginette Girard
Keyword(s):  
Protein Content ◽  
Acinar Cell ◽  
Dna Content ◽  
Pancreatic Tissue ◽  
Weight Basis ◽  
Secretory Proteins ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Rat Pancreas

Recent observations have confirmed the existence of more than one pool of secretory proteins in the rat pancreatic tissue. To determine if these different pools could be located in the different regions of the rat pancreas, the amylase and chymotrypsin contents have been measured in the biliary, duodenal, gastric, and splenic regions. On a tissue DNA content, protein content, or a fresh weight basis, the proportions of these two enzymes are comparable in the four regions. It is therefore postulated that heterogeneity of enzyme composition exists either within these regions or within the acinar cell itself.

scholarly journals Adaptación y calidad tecnológica de la variedad de frijol Negro Tacana.

2016 ◽  
Vol 7 (1) ◽  
pp. 26 ◽  
Author(s):  
Ernesto López ◽  
Noé Becerra ◽  
Octavio Cano ◽  
Dora Zaleta ◽  
Jorge Acosta
Keyword(s):  
Protein Content ◽  
Dry Weight ◽  
Humid Tropics ◽  
Weight Basis ◽  
Fiber Content ◽  
Experimental Conditions ◽  
Yield Trial ◽  
Wide Adaptation ◽  
Stable Yield

In this paper the results obtained with a uniform yield trial including 22 bean genotypes grown in three states of Mexico are presented. Under experimental conditions, bred line DOR 390 resulted outstanding for its wide adaptation, high and stable yield (1041 kg/ha, bi=1.0 Sd2i=0) Under farmer’s conditions, DOR 390 (1214 kg/ha) was superior to commercial cvs, Negro Cotaxtla 91 (1142 kg/ha) and Jamapa (867 kg/ha); at neight locations of the humid tropics in the States of Tamaulipas, Veracruz and Chiapas, DOR 390 proved to be highly resistant in comparison to Negro Huasteco 81. Comparing Dor 390 to bred cvs. and landraces of the same commercial class, tropical black, Dor 390 showed and average protein content of 25%, on a dry weight basis, and the highest fiber content of the seven genotypes evaluated. DOR 390 was registered as a new cultivar in 1993, and made available to the farmers of the humid tropics in 1994, under the name of “Negro Tacana”.

scholarly journals Proximate Analysis and Mineral Composition of Commercially Important Spiny Lobsters from Visakhapatnam Coast, Andhra Pradesh, India

2021 ◽  
pp. 39-47
Author(s):  
Pavan Kumar Kommuri ◽  
Naresh Mugada ◽  
Ramesh Babu Kondamudi
Keyword(s):  
Protein Content ◽  
Andhra Pradesh ◽  
Research Work ◽  
Dry Weight ◽  
Proximate Analysis ◽  
Weight Basis ◽  
Spiny Lobsters ◽  
Moisture Percentage ◽  
Commercially Important ◽  
Visakhapatnam Coast

The research work was carried out to assess the dietary qualities of spiny lobsters collected from Visakhapatnam fishing harbour, situated on the northeast coast of Andhra Pradesh. The species selected were Panulirus homarus (Linnaeus, 1758), P. ornatus (Fabricius, 1798), P. versicolor (Latreille, 1804), and P. polyphagus (Herbst, 1793). The species were morphometrically measured, and their tissue was analyzed for the comparative proximate composition (Moisture, Protein, Lipid, and Ash) on a dry weight basis. Protein content was found to be high in all the lobster species. Highest protein content was observed in P. homarus (mg/g), followed by P. ornatus and P. versicolor (mg/g). Moisture percentage was 72.7, 75.7, 76.2, and 74.8% respectively. In addition, four minerals were analysed (Ca, Na, K, and Mg) in the present study. Calcium was observed maximum in all the species, and Magnesium was recorded minimum in all the lobsters.

scholarly journals Toxic affects of chromium on some quality parameters of Sorghum bicolor (L.)

2018 ◽  
Vol 10 (1) ◽  
pp. 122-127 ◽  
Author(s):  
Sweety Sihag ◽  
U. N. Joshi
Keyword(s):  
Sorghum Bicolor ◽  
Protein Content ◽  
Chlorophyll Content ◽  
Potassium Dichromate ◽  
Dry Weight ◽  
Quality Parameters ◽  
Weight Basis ◽  
Growth Stages ◽  
Fresh Weight ◽  
Sorghum Bicolor L

A pot experiment was conducted to determine the effects of varying Cr (VI) levels [0.0–4.0 mg Cr (VI) kg-1 soil in the form of potassium dichromate] on the some quality parameters of sorghum Sorghum bicolor (L.). Quality parameters was measured in terms of protein content, chlorophyll content, and IVDMD ( In vitro dry matter digestibility) content at different growth stages, i.e. 35 DAS, 70 DAS and 90 DAS (Days after sowing) that were adversely affected with an increase in Cr (VI) levels from 0.0 to 4.0 mg Cr (VI) kg-1 soil. The decline in protein content 4.67 g/ kg dry weight at T4 was observed as compared to control (T1) ( 8.96 g/kg dry weight basis) at 35 DAS in leaves , Total chlorophyll content declined from 3.25 mg g-1 fresh weight (T1) to 2.40 mg g-1 fresh weight (T4) at 35 DAS and IVDMD content declined from 43.60 to 33.60 per cent dry weight basis with increment in chromium concentration. It is concluded that Cr (VI) at higher doses (4.0 mg Cr (VI) kg-1 soil) adversly affects the quality parameters of Forage sorghum Sorghum bicolor (L.) however, quality parameters are responsible for nutritive value of sorghum Sorghum bicolor (L.).

THE PHOSPHORUS FRACTIONS OF BACILLUS CEREUS AND BACILLUS MEGATERIUM: I. A COMPARISON OF SPORES AND VEGETATIVE CELLS

1955 ◽  
Vol 1 (7) ◽  
pp. 502-519 ◽  
Author(s):  
P. C. Fitz-James
Keyword(s):  
Nucleic Acid ◽  
Total Phosphorus ◽  
Cell Walls ◽  
Dry Weight ◽  
Phosphorus Fractions ◽  
Weight Basis ◽  
Insoluble Residue ◽  
Vegetative Cells ◽  
Acid Labile ◽  
Total P

The phosphorus fractions of the spores and young vegetative cells of B. cereus and B. megaterium were compared using the methods of Schneider and of Schmidt and Thannhauser. Differences were found, not only between the two types of cell, but also between the species studied. The spores of B. cereus and B. megaterium contained, on a dry weight basis, similar amounts of nucleic acid, yet the spores of B. megaterium contained twice as much total phosphorus as those of B. cereus. The excess phosphorus of B. megaterium spores was present as an acid and alkali insoluble residue and was made up of empty spore coats. While this same fraction accounted for only 4% of the total phosphorus of B. cereus spores it made up some 60% of that of B. megaterium. A similar fraction from vegetative cells was low in phosphorus. The spore coats of B. megaterium, in contrast to the cell walls were lysozyme-resistant. Cold acid-soluble and lipid phosphorus could be properly estimated only on disrupted spores. Disruption also proved essential for the ready extraction of spore nucleic acids with hot TCA, but did not greatly alter the solubility of the residue phosphorus. Ribosenucleic acid (RNA) comprised 50% of the total phosphorus in the spores of B. cereus (3–4% of the dry weight) but only 25% in B. megaterium spores. In both species the RNA of the vegetative forms accounted for a larger proportion of the total P of the cell. Ribonuclease digested the RNA of spores and vegetative cells to the same degree. The Schmidt and Thannhauser method was found more suitable than the Schneider method for the estimation of desoxyribosenucleic acid (DNA). The DNA content of B. cereus spores was about 1% of their dry weight; that of B. megaterium spores was slightly less. Some 12–20% of the phosphorus of B. cereus spores and 6% of that of the young vegetative cells was present as acid-labile (non-nucleic acid) phosphorus which exhibited some of the characteristics of polymetaphosphate.

Composition of Methanospirillum hungatii GP1 during growth on different media

1980 ◽  
Vol 26 (5) ◽  
pp. 577-582 ◽  
Author(s):  
C. Breuil ◽  
G. B. Patel
Keyword(s):  
Protein Content ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Synthetic Medium ◽  
Density Measurement ◽  
Dry Weight ◽  
Weight Basis ◽  
Total Carbohydrate ◽  
Mineral Salts

Growth of Methanospirillum hungatii GP1 as determined by optical density measurement was comparable to growth assessed by cell dry weight, ribonucleic acid content, and deoxyribonucleic acid content. Cultivation of M. hungatii on synthetic medium containing mineral salts, vitamins, and acetic acid indicated that, on a dry weight basis, cell constituents such as protein (71%), ribonucleic acid (15.8%), deoxyribonucleic acid (1.6%), and total carbohydrate (3.2%) did not vary significantly with the growth phase. Cells grown in the synthetic medium supplemented with yeast extract and tryptone had slightly higher protein content (76%), but the concentrations of the other cell constituents were similar and did not fluctuate much during growth. Nitrogen limiting growth resulted in somewhat lower ribonucleic acid content as well as slightly higher protein content than that in cells grown in nonlimiting medium. Methanospirillum hungatii did not accumulate any of the commonly known reserve materials under nitrogen or carbon and hydrogen limiting growth.

scholarly journals Effect of Wire Supports on Pod Yield of Winged Beans (Psophocarpus tetragonolobus (L.) DC.

1969 ◽  
Vol 64 (2) ◽  
pp. 211-218
Author(s):  
R. Del Valle, Jr. ◽  
M. A. Lugo-López ◽  
T. W. Scott
Keyword(s):  
Protein Content ◽  
Growth And Development ◽  
Dry Weight ◽  
Weight Basis ◽  
Psophocarpus Tetragonolobus ◽  
Soil Humidity ◽  
Pod Yield ◽  
Significant Difference ◽  
Tuber Production ◽  
Pod Growth

An experiment with winged beans (Psophocarpus tetragonolobus (L.) (DC.) was conducted in a Torres clay (Plinthic Palehumults) to evaluate the performance of WB 21-8 and WB 10-3, and the effect of using wire supports on growth and development, fresh pod yield and tuber production. Without wire supports, there were no differences between cultivars either in growth and development or in fresh pod yields. There was, however, a highly significant difference between cultivars when both were grown with wire supports. A significant difference between yields of plants with and without wire supports within cultivar WB 21-8 was evident, while no differences were recorded for cultivar WB 1 10-3. The interaction of cultivars and use of wire supports was significant. Fresh pod yields were consistently low for both cultivars when supports were omitted, probably due to pod rot as result of the high soil humidity during pod growth. A field-wide average of 5.7 t/ha was obtained from only three harvests. There were no significant differences in protein content between treatments. A mean protein content (dry weight basis) of 20% was recorded. Tuber samples (irrespective of cultivar) had a mean protein content of 10%.

NUCLEIC ACID AND PROTEIN CONTENT IN THE PARS DISTALIS OF THE PITUITARY AND HYPOTHALAMUS OF THE FROG, RANA ESCULENTA: EFFECTS OF GONADECTOMY AND CAPTIVITY

1972 ◽  
Vol 55 (3) ◽  
pp. 471-477 ◽  
Author(s):  
R. K. RASTOGI ◽  
G. CHIEFFI
Keyword(s):  
Cerebral Cortex ◽  
Nucleic Acid ◽  
Protein Content ◽  
Dna Content ◽  
Rana Esculenta ◽  
Pars Distalis ◽  
Weight Changes ◽  
Short Term ◽  
Rna And Dna

SUMMARY The protein, RNA and DNA content was measured in the pars distalis, hypothalamus and cerebral cortex of gonadectomized frogs (Rana esculenta) and compared with the levels in captive and in wild controls. Short-term (35 days) gonadectomy increased the weight of the pars distalis and also its RNA and protein content, whilst gonadectomy for a longer period (135 days, females only) depressed these parameters below control levels. In the hypothalamus, short-term gonadectomy increased the protein concentration, and long-term gonadectomy the RNA content. Captivity alone for 135 days caused a diminution in the weight and protein content of the pars distalis, although the various parameters showed an increase which correlated with the annual reproductive cycle. The DNA content (μg/mg tissue) of the pars distalis was similar in all groups suggesting that any weight changes were not due to changes in cell numbers. Neither gonadectomy nor captivity altered the nucleic acid and protein content of the cerebral cortex. The pars distalis weighed more in female frogs and contained more RNA and protein per mg.

Aspects of metabolic development in an illuminated synchronous culture of Scenedesmus obtusiusculus

1973 ◽  
Vol 51 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Gaurangakumar Das
Keyword(s):  
Growth Phase ◽  
Ribonucleic Acid ◽  
Culture Medium ◽  
Deoxyribonucleic Acid ◽  
Dry Weight ◽  
Light Period ◽  
Weight Basis ◽  
Lipid Phosphorus ◽  
Acid Phosphate ◽  
Cellular Development

Developing synchronous cells of Scenedesmus obtusiusculus Chod. were investigated during the 15-h light period of the 24-h life cycle. Measurements were made of photosynthesis and of the chlorophyll, carotene, nucleic acid, and protein content, including intracellular phosphorus.Photosynthetic oxygen evolution (μmol/mg chlorophyll) increased during the early growing stage and declined gradually in the older cells. Chlorophyll, carotene, protein, ribonucleic acid, and ribonucleic acid phosphate of these cells increased during the growth phase (dry weight basis) and declined in the sporulating phase, whereas the deoxyribonucleic acid and deoxyribonucleic phosphate decreased during the growth phase. Deoxyribonucleic acid increased slightly in the sporulating phase, but deoxyribonucleic acid phosphate remained relatively constant during this period. When calculated on a per cell basis, ribonucleic acid and ribonucleic acid phosphate increased steadily throughout the period of observation, whereas deoxyribonucleic acid and deoxyribonucleic acid phosphate remained constant during the first 5 h and then increased gradually. Analysis of the intracellular distribution of phosphorus(dry weight basis) revealed that methanol-soluble (lipid) phosphorus decreased steadily with cell growth, whereas the content of acid-soluble phosphorus and ethanol–ether-soluble (lipid) phosphorus fluctuated markedly. The pH of the culture medium increased concomitantly with cellular development during the light period.

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