The influence of carbon dioxide on protein synthesis in etiolated coleoptiles of Arena sativa
Various concentrations of carbon dioxide have been used to assess the influence of carbon dioxide on protein metabolism in etiolated coleoptiles of Avena sativa. Increases in carbon dioxide concentration from 0% to 0.03% to 3% result in dramatic increases in both the total level of protein and the incorporation of radioactive leucine into protein. In addition an electrophoretic analysis indicates that as the carbon dioxide concentration is raised from 0% to 0.03% there is both an increased synthesis of most proteins plus a pronounced synthesis of one particular protein fraction. These results indicate that the normal atmospheric concentration of carbon dioxide has a profound influence on protein metabolism in Avena sativa coleoptiles and are discussed in connection with the well-documented phenomenon of carbon dioxide stimulated growth in etiolated plant tissues.